신부전이 동반된 당뇨병 환자에서 발생된 침습성 모균증 : 수술과 Liposomal amphotericin B 및 GM-CSF 병합 요법에 의한 성공적인 치험 1예

이원영,오기원,임국희,장재혁,이동건,최정현,강무일,신완식,차봉연,이광우,손호영,강성구 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.4

저자들은 신부전이 동반된 당뇨병 환자에서 발생된 부비동형 모균증에 대하여 수술과 함께 liposomal amphotericin B 및 GM-CSF의 복합치료를 하여 성공적으로 치료한 1예를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. Mucormycosis (zygomycosis) primarily affects diabetic or immunocompromised patients and typically progresses rapidly, necessitating surgical excision and antifungal therapy with amphotericin B. Large doses of amphotericin B are needed for cure, but it has the risk of causing significant renal toxicity. The recent development of liposomal amphotericin B allows antifungal therapy to be administered with potentially improved efficacy and reduced nephrotoxicity. We have experienced a case of paranasal mucormycosis successfully treated with surgery, liposomal amphotericin B and GM-CSF. A 59-year-old male suffering from diabetes mellitus for 6 years was admitted with pain at left maxillary area. He was diagnosed as mucormycosis after cytologic exam on the necrotic nasal mucosa, which showed typical hyphae. He have had diabetic nephropathy with macroproteinuria and had rapidly rising serum creatinine levels with the amphotericin B treatment: creatinine levels reverted to basal level with the use of liposomal amphotericin B. Despite surgical excision and continued antifungal therapy, his infection was not effectively controlled. Therefore, GM-CSF was administered additionally to improve phagocytic activity of leukocytes. He was finally cured after receiving a combination of aggressive surgery, liposomal amphotericin B and GM-CSF. To our knowledge, this is the first detailed clinical description of the treatment of mucormycosis with liposomal amphotericin B in Korea.

보리등겨로 제조한 간장의 향기성분

권오준,이은정,최웅규,손동화,이석일,정연건,지원대 한국위생과학회 2002 한국위생과학회지 Vol.8 No.2

새로운 장류제품으로서 보리등겨의 이용방안을 모색하기 위하여 간장을 만들어 연구하였다. 보리로 제조한 간장의 갈색화는 점차적으로 증가 하였으며 완만한 변화를 보였다. 향기성분으로는 4-vinyl-2-methoxy-phenol, benzeneacetaldehyde, palmitic acid, 2-furancatboxaldehyde, methyl-9, 12-octadecadienoate, di-(2-ethylhexyl)phthalate, diethyl phtalate, dibytyl-1,2-benzenedicatboxylate, 5-methyl-2-furancarboxaldehyde, 3,4-dimethyl-1h-pyrazole, phenylethyl alcohol, dioctyl-hexanedioate, dimethyl-1,2-benzenedicatboxylate, benzaldehyde, methional, 2-methoxy-phenol, n-furfurylidene-3-methylbutyl amine, 1-furfuryl-2-formyl pyrrole, tetradrcanoic acid, 5-methyl-pyrimidine, 4-methyl-5-hydroxymethyl-imidazole, maltol, 5-(5-methyl-2-furanyl)methyl-2-furancarboxaldehyde 순으로 높은 함량을 차지 하였다. For investigation of new utilization as jang-products, kanjang was prepared using barely bran. This study was conducted to investigate flavor components of kanjang during fermentation time. The optical density was gradually increased. Among the flavor components identified in kanjang made with barley bran, the contents of 4-viny1-2-methoxy-phenol was the most in quantity followed by benzeneacetaldehyde, palmitic acid, 2-furancarboxaldehyde, methyl-9,12-octadecadienoate, di-(2-ethylhexyl)phthalate, diethyl phtalate, dibutyl-1,2-benzendicarboxylate, 5-methyl-2-furancarboxaldehyde, 3,4-dimethyl-1h-pyrazole, phenylethyl alcohol, dioctyl-hexanedioate, dimethyl-1,2-benzenedicarboxylate, benzaldehyde, methional, 2-methoxy-phenol, n-furfurylidene-3-methylbutyl amine, 1-furfuryl-2-formyl pyrrole, tetradecanoic acid, 5-methyl-pyrimidine, 4-methyl-5-hydroxymethyl-imidazole, maltol and 5-(5-methyl-2-furanyl)methyl-2-furancarboxaldegyde.

국내 대학병원에서 정주용 Ciprofloxacin 사용의 적정성 평가

김신우,박효정,오원섭,김연숙,장현하,정숙인,정두련,이혁,염준섭,기현균,손준성,백경란,우건조,송재훈 대한감염학회 2004 감염과 화학요법 Vol.36 No.6

목적 : 국내 병원에서의 정주 ciprofloxacin 사용의 적정성을 평가하고자 하였다. 재료 및 방법 : 2003년 9월 25일부터 8개의 대학병원에서 정주 ciprofloxacin을 받았던 290명의 연속적으로 모집한 성인환자를 대상으로 약제사용 평가(DUE)를 전향적으로 수행하였다. 이 연구에 사용된 DUE 기준은 미국병원약사회의 기준을 국내 실정에 맡게 수정하여 사용하였으며 1) 사용의 정당성에 관한 항목, 2) 사용 시 준수되거나 시행되어야 할 사항에 대한 항목, 3) 부작용에 대한 항목, 4) 치료 효과에 대한 항목으로 나누어 조사하였다. 결과 : 환자의 평균 나이는 54세였다. 33예(26.9%)에서만 약제 사용의 적정성 측면에서 기준을 만족하였고 91예(73.1%)에서는 사용이 적절하지 않았다. 정주 cipro-floxacin의 부적절한 사용의 흔한 원인은 수술 전후의 예방약 사용, 비뇨기계 처치 전의 예방적 사용, 열성 질환에 대한 경험적 사용 등의 순이었다. 사용 시 준수사항 의 측면에서는 사용 전 배양검사(73.8%)와 사용 중 배양검사(68.6%)가 낮은 수행 정도를 보였으나 그 외는 비교적 높은 적정성(82.4-100%)을 보여주었다. 정주 ciprofloxacin사용의 합병증은 드물었다. 치료 효과의 면에서 치료반응은 93.3%였다. 결론 : 약제 사용의 정당성 기준에 따른 정주 cipro-floxacin의 부적절한 사용은 연구에 포함된 대학병원에서 매우 흔하였다(73.1%). 그러므로 적절한 항생제 사용에 대한 교육과 제도적 장치가 매우 필요한 것으로 판단되었다. Background : To evaluate the appropriateness of intravenous (IV) ciprofloxacin usage in university hospitals in Korea. Materials and Methods : We prospectively evaluated the appropriateness of drug usage in 290 consecutive adult patients who received IV ciprofloxacin in eight teaching hospitals during the period from September 25, 2003 to November 15, 2003. Drug use evaluation (DUE) in this study was based on the modified standard by the American Society of Hospital Pharmacy with regard to the justification of drug use, critical and process indications, complications, and outcome measures. Results : Average age of the patients was 54 years. The use of IV ciprofloxacin was appropriate only in 33 cases (26.9%). Common reasons for inappropriate use of IV ciprofloxacin include perioperative uses or prophylactic injection before urologic procedures, and empirical therapy for febrile episodes. Most of the "critical indications" and "process indications" showed high rate of appropriateness (82.4-100%). However, the rate of taking culture and sensitivity test before (73.8%) and during ciprofloxacin use (68.6%) were low. Complications with ciprofloxacin use were rare. In 93.3% of cases, ciprofloxacin treatment was clinically effective. Conclusion : Use of intravenous ciprofloxacin is frequently inappropriate in tertiary care hospitals in Korea. Implementation of education program and antibiotic control system is critical in improving the appropriateness of antimicrobial use.

SO3 로 술폰화된 Polyphenylene Sulfide 의 이온교환능

손원근(Won Gun Son),문선모(Sun Mo Moon),백중현(Joong Hyun Baek),김동철(Dong Chul Kim),박정기(Jung Ki Park),송해영(Hae Young Song) 한국고분자학회 1995 폴리머 Vol.19 No.4

전기방사를 이용한 Pullulan Electrospun Fiber Webs의 제조 및 특성

손태원(Tae Won Son),이건민(Gun Min Lee),이동원(Dong Won Lee),이주현(Ju Hyun Lee),임학상(Hak Sang Lim) 한국고분자학회 2012 폴리머 Vol.36 No.2

전기방사는 휘발성 용매에 녹아있는 다양한 물질들을 마이크로 크기의 섬유로 제조하는데 사용되고 있다. 이 번 연구에서는 물을 용매로 사용하여 pullulan을 전기 방사하였다. 부드러운 섬유를 얻기 위하여 pullulan 농도와 전 압을 최적의 조건으로 설정하였다. Pullulan 농도는 pullulan 용액의 점도와 표면 장력에 큰 영향을 받는다. Bead 형태 의 pullulan 전기방사 섬유는 5 wt%이하의 농도에서 얻어진다. Pullulan 용액의 농도가 10 wt%로 유지되고, 전압이 15 kV에 고정되었을 때, pullulan 섬유의 평균 직경은 200 nm로 감소하였다. Pullulan electrospun 섬유는 높은 용해도, 유연성, 부드러움과 강한 접착성을 보여준다. Electrospinning is a versatile process used to prepare micro or nano sized fibers from various materials dissolved in volatile solvents. This study reports electrospun pullulan fibrous webs fabricated through electrospinning using water as a solvent. The electrospinning conditions such as pullulan (PUL) concentration and applied voltage were optimized in order to obtain smooth electrospun fibers. The concentration of PUL greatly influenced the viscosity and surface tension of PUL solution. PUL beaded electrospun fibers were obtained from PUL solutions with concentrations lower than 5 wt%, while homogenous electrospun fibers were prepared from solutions with high concentration and high viscosity. The average diameters of PUL fibers were decreased to 200 nm when the polymer concentration was kept at 10 wt% and the applied voltage was fixed at 15 kV during electrospinning. PUL electrospun fiber exhibited higher solubility, flexibility, softness and adhesive strength.

Dendrimer porphyrin-terminated polyelectrolyte multilayer micropatterns for a protein microarray with enhanced sensitivity

Son, Kyung Jin,Kim, Suhyun,Kim, Joo-Ho,Jang, Woo-Dong,Lee, Yeol,Koh, Won-Gun Royal Society of Chemistry 2010 Journal of materials chemistry Vol.20 No.31

<P>Through a combination of layer-by-layer (LbL) self-assembly (SA) and lift-off methods, a dendrimer-coated polyelectrolyte multilayer micropattern was prepared for protein microarrays. A silicon substrate was patterned with a photoresist thin film using conventional photolithography, and then poly(ethyleneimine) (PEI) and poly(sodium 4-styrenesulfonate) (PSS) were alternatively deposited onto the substrate surface using spin-assisted self-assembly. A well-defined multilayer microarray was produced by subsequent removal of the photoresist template by a lift-off process. Dendrimer porphyrin (DP) was successively immobilized onto the PEI-terminated micropatterns <I>via</I> electrostatic interactions between the negatively-charged DPs and positively-charged PEI segments. Because of strong fluorescence from focal porphyrins, the homogeneous covering of DPs onto the multilayer micropatterns was easily confirmed using fluorescence microscopy. Atomic force microscopy (AFM) also showed morphological change of micropatterned surfaces by DP immobilization. Based on these results, IgG was immobilized on the DP-coated protein microarrays, and immunoassays were performed to demonstrate that the DP-coated microarrays yielded a higher fluorescence signal and were more sensitive than the control microarrays that were coated with linear PAA polymer instead of DP due to the multiple functional groups present on the DP-coated arrays and their increased surface area relative to control microarrays.</P> <P>Graphic Abstract</P><P>A protein microarray prepared by combining multilayer micropatterns and dendrimer porphyrin exhibited drastically improved sensitivity for immunosensor application. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c0jm00498g'> </P>

Effect of Circuit Training on Aging-related Hormones in Obese Middle-aged Women

Won-Mok Son(손원목),Yi-Sub Kwak(곽이섭),Gun-Do Kim(김군도),Min-Sung Ha(하민성),Song-Young Park(박송영),Gi-Dong Sung(성기동) 한국생명과학회 2017 생명과학회지 Vol.27 No.9

노화 과정에 따라 중년 여성들은 폐경 이후 체력이 저하되기 시작하면서 체지방 증가와 제지방량의 감소로 비만으로 이어지기 쉽고, 이러한 현상은 체내 호르몬의 분비의 변화와 함께 발생된다. 본 연구는 서킷 트레이닝이 비만 중년 여성의 노화 관련 호르몬에 미치는 영향을 구명하기 위해 실시하였다. 대상자 체지방률 30% 이상인 비만 중년 여성 운동군 10명, 대조군 10명 총 20명을 실시하였다. 운동 시간은 준비운동 5분, 본 운동 20분, 정리운동 5분으로 총 30분 주 3회 실시하였으며, 운동 강도는 운동자각도를 이용하여 13~17(약간 힘들다-매우 힘들다)수준으로 실시하였고, 또한 POLAR 심박수 변화량을 측정하여 1~6주는 60~70%HRR (Heart rate reserve), 7~12주는 70~80%HRR로 설정하였다. IGF-1은 운동군이 유의하게 증가하였으며, GH는 운동군이 유의하게 증가하였고, DHEA-S는 운동군이 유의하게 증가하였다. 이상의 결과 서킷 트레이닝이 비만 중년 여성의 노화 관련 호르몬에 긍정적인 영향을 준 것으로 사료된다. Due to aging, the physical fitness of middle-aged women decreases after menopause. This results in increased body fat and reduced lean body mass, both of which can lead to obesity. This phenomenon is accompanied by changes in hormone secretion in the body. The purpose of this study was to analyze the effects of circuit training on aging-related hormones in obese middle-aged women. The subjects were 20 obese middle-aged women. The subjects were divided into two groups: a circuit training group (n=10) and nonexercise control group (n=10). Growth hormone (GH), insulin-like growth factor-1 (IGF-1), estrogen, and DHEA-S were measured before and after the circuit training program. The circuit training group performed circuit training for 12 weeks, three times per week. Exercise intensity was increased gradually from 60% to 80% of heart rate reserve every 6 weeks. In the circuit training group, at the end of the 12-week training program, the IGF-1 levels were significantly increased compared to pre-exercise levels, and they were higher than those of the control group. GH levels were also significantly increased in the circuit training group, but the differences were not statistically significant. DHEA-S was significantly increased in the circuit training group, but the difference was not statistically significant. The results showed that circuit training improved aging-related hormones levels in obese middle-aged women.

Graft Copolymer-Templated Mesoporous TiO₂ Films Micropatterned with Poly(ethylene glycol) Hydrogel: Novel Platform for Highly Sensitive Protein Microarrays

Son, Kyung Jin,Ahn, Sung Hoon,Kim, Jong Hak,Koh, Won-Gun American Chemical Society 2011 ACS APPLIED MATERIALS & INTERFACES Vol.3 No.2

<P>In this study, we describe the use of organized mesoporous titanium oxide (TiO<SUB>2</SUB>) films as three-dimensional templates for protein microarrays with enhanced protein loading capacity and detection sensitivity. Multilayered mesoporous TiO<SUB>2</SUB> films with high porosity and good connectivity were synthesized using a graft copolymer consisting of a poly(vinyl chloride) (PVC) backbone and poly(oxyethylene methacrylate) (POEM) side chains as a structure-directing template. The average pore size and thickness of the TiO<SUB>2</SUB> films were 50-70 nm and 1.5 μm, respectively. Proteins were covalently immobilized onto mesoporous TiO<SUB>2</SUB> film via 3-aminopropyltriethoxysilane (APTES), and protein loading onto TiO<SUB>2</SUB> films was about four times greater than on planar glass substrates, which consequently improved the protein activity. Micropatterned mesoporous TiO<SUB>2</SUB> substrates were prepared by fabricating poly(ethylene glycol) (PEG) hydrogel microstructures on TiO<SUB>2</SUB> films using photolithography. Because of non-adhesiveness of PEG hydrogel towards proteins, proteins were selectively immobilized onto surface-modified mesoporous TiO<SUB>2</SUB> region, creating protein microarray. Specific binding assay between streptavidin/biotin and between PSA/anti-PSA demonstrated that the mesoporous TiO<SUB>2</SUB>-based protein microarrays yielded higher fluorescence signals and were more sensitive with lower detection limits than microarrays based on planar glass slides.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2011/aamick.2011.3.issue-2/am101141z/production/images/medium/am-2010-01141z_0011.gif'></P>

Water Saving by Shallow Intermittent Irrigation and Growth of Rice

Gun Won, Jong,Soo Choi, Jang,Phil Lee, Seung,Ho Son, Seung,Ok Chung, Sang The Crop Science Society of Japan 2005 Plant production science Vol.8 No.4

<P>To reduce water requirement and improve water productivity (the grain yield per unit volume of water irrigated) by water-saving irrigation techniques, we examined the effects of very shallow intermittent irrigation (VSII, 2cm), shallow intermittent irrigation (SII, 4cm) and traditional deep water irrigation (DWI, 10cm) on rice growth and yield in the field for two years. The amount of water irrigation during the rice-growing period (average of two years) was 318, 391 and 469 mm in VSII, SII and DWI, respectively. Rice growth and grain yield were not significantly influenced by the treatments. As the irrigation water input decreased, the water productivity increased. The water productivity increased by 46 % in VSII and 20 % in SII on the average as compared with DWI. The shallower the irrigation depth, the lower the breaking weight and the higher the lodging resistance, and the deeper the roots in the paddy soil. In DWI, the percentage of head rice was lower and the protein content was higher, suggesting deterioration in the palatability of cooked rice due to the increase of chalky rice. The water-saving rate was 32.9 % in VSII and 17.2 % in SII as compared with typical deep water irrigation in Korea.</P>

Life Science : Research Article ; Effects of silicone-Based Gels Containing Allantoin, Dexpanthenol and Heparin on Hypertrophic Scarring in the Rabbit Ear Model

( Gun Gook Kim ),( Sae Kwang Ku ),( Dong Won Lee ),( Nirmal Marasini ),( Roshan Pradhan ),( Mi Won Son ),( Joon Ho Jun ) 영남대학교 약품개발연구소 2012 영남대학교 약품개발연구소 연구업적집 Vol.22 No.0

Silicone-based formulations are extensively used for the management of hypertrophic scars. Although the exact mechanism of action is still unknown, it has been postulated that some occlusion and hydration of the stratum corneum with subsequent cytokine-mediated signaling from keratinocvtes to dermal fibroblasts is involved in its antiscarring effects. In this study, the effectiveness of silicone-based gels containing allantoin, dexpanthenol, and heparin was evaluated for improving the healing of hypertrophic scars. It was found that silicone-based gels showed remarkable improvements in hypertrophic scar healing and low amounts of skin pigmentation the rabbit ear model compared with the nontreated control or base alone. Furthermore, the histopathololgical and histomorphometrical profiles of three different formu-lations containing 1%, 5%, and 20% silicone contents exhibited marked or significant decreases in the scar elevation index, anterior skin and epithelial thicknesses, inflammatory cells, vessels, collagen disor-ganization, and fiborblasts compared with nontreated control hypertrophic sacrs. Therefore, these results indicate that silicone-based gels containing heparin, allantoin, and dexpanthenol could be promising formulations for the healing of hypertrophic scars, Drug Dey Res 73 ; 146-153, 2012.