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<i>In Vitro</i> Biosynthesis of Metal Nanoparticles in Microdroplets
Lee, Kyoung G.,Hong, Jongin,Wang, Kye Won,Heo, Nam Su,Kim, Do Hyun,Lee, Sang Yup,Lee, Seok Jae,Park, Tae Jung American Chemical Society 2012 ACS NANO Vol.6 No.8
<P>We report the use of a hydrogel polymer, recombinant <I>Escherichia coli</I> cell extracts, and a microdroplet-based microfluidic device to fabricate artificial cellular bioreactors which act as reactors to synthesize diverse metal nanoparticles (NPs). The combination of cell extracts, microdroplet-based microfluidic device, and hydrogel was able to produce a mass amount of artificial cellular bioreactors with uniform size and shape. For the first time, we report the alternating generation of microdroplets through one orifice for the fabrication of the artificial cellular reactors using the cell extract as inner cellular components and hydrogel as an artificial cellular membrane. Notably, the hydrogels were able to protect the encapsulated cell extracts from the surrounding environment and maintain the functionality of cellular component for the further cellular bioreactor applications. Furthermore, the successful applications of the fabricated artificial cellular bioreactors to synthesize various NPs including quantum dots, iron, and gold was demonstrated. By employing this microfluidic technique, the artificial cellular bioreactors could be applicable for the synthesis of diverse metal NPs through simple dipping of the reactors to the metal precursor solutions. Thus, the different size of NPs can be synthesized through controlling the concentration of metal precursors. This artificial cellular bioreactors offer promising abilities to biofriendly ways to synthesis diverse NPs and can be applicable in chemical, biomedical, and bioengineering applications.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancac3/2012/ancac3.2012.6.issue-8/nn302043q/production/images/medium/nn-2012-02043q_0001.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nn302043q'>ACS Electronic Supporting Info</A></P>
Wang, Cai Yi,Kim, Donghwa,Zhu, Yan Kui,Oh, Dong-Chan,Huang, Ri Zhen,Wang, Heng-Shan,Liang, Dong,Lee, Sang Kook American Society of Pharmacognosy 2019 Journal of natural products Vol.82 No.11
<P>Three new germacrane sesquiterpenoid-type alkaloids with an unusual Δ<SUP>8</SUP>-7,12-lactam moiety, glechomanamides A-C (<B>1</B>-<B>3</B>), and two pairs of 7,12-hemiketal sesquiterpenoid epimers (<B>4a</B>/<B>b</B>, <B>5a</B>/<B>b</B>) were isolated from <I>Salvia scapiformis</I>. Their structures were elucidated by spectroscopic methods including HRESIMS, IR, UV, and 1D and 2D NMR and also confirmed by single-crystal X-ray diffraction analysis. The chemical transformation of compounds <B>1</B>-<B>5</B> in a solution environment was analyzed by 2D NMR spectroscopy. The aza acetallactams (<B>1</B>-<B>3</B>) were stable in organic solvent, while single crystals of the hemiacetal esters (<B>4a</B>/<B>b</B>, <B>5a</B>/<B>b</B>) underwent a tautomeric equilibrium after being dissolved. Single crystals of <B>4a</B>, <B>4b</B>, and <B>5a</B> were obtained for the first time as their naturally occurring forms. Glechomanamide B (<B>2</B>) exhibited antiangiogenic activity by suppression of vascular endothelial growth factor (VEGF)-induced tube formation through modulation of VEGF receptor 2 (VEGFR2)-mediated signaling pathways in human umbilical vascular endothelial cells (HUVECs). In addition, compound <B>2</B> also showed the significant suppression of mRNA expression associated with glycolysis and angiogenesis biomarkers in high glucose (30 mM)-induced HUVECs. These findings suggest that compound <B>2</B> might be a potential lead compound candidate for the management of diabetic retinopathy.</P> [FIG OMISSION]</BR>
Lee, Sang-Bum,Lee, Kyung-Won,Wang, Tao,Lee, Jae-Sung,Jung, U-Suk,Nejad, Jalil Ghassemi,Oh, Young-Kyoon,Baek, Youl-Chang,Kim, Kyoung Hoon,Lee, Hong-Gu Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.1
Objective: This study investigated the effects of oral administration of rumen-protected L-tryptophan (RPL-T) on duodenal starch digestion and gastrointestinal hormones (GIH) secretions using Hanwoo beef steers as the animal models. Methods: Four steers (423±24 kg) fitted with ruminal and duodenal cannulas were employed in a crossover design replicated twice. Treatments were control (basal diet) and RPL-T (basal diet+191.1 mg/kg body weight [BW]) group. Blood and duodenal samples were collected to measure serum GIH levels and pancreatic α-amylase activity at day 0, 1, 3, and 5 (-30, 30, 90, 150, and 210 min) of the study. Samples from each segment of the gastrointestinal tract were collected via ruminal and duodenal cannulas and were used to determine soluble protein and the starch digestion rate at days 6 (-30, 180, 360, and 540 min) and 8 (-30, 90, 270, and 450 min) of the experiment. Results: No significant difference in ruminal pH, NH<sub>3</sub>-N, and total volatile fatty acid including the levels of acetate, propionate, butyrate, isobutyrate, valerate, isovalerate, and the acetate-to-propionate ratio was observed between groups (p>0.05). Crude protein uptake was higher and feces starch content was lower in RPL-T group than the control group (p<0.05). The D-glucose contents of feces in RPL-T group decreased at day 5 compared to those in the control group (p<0.05), however, no change was found at day 0, 1, or 3 compared to the control group (p>0.05). Serum cholecystokinin (CCK), melatonin, duodenal pancreatic α-amylase activity, and starch digestion were significantly higher in RPL-T group than the control group (p<0.05). Conclusion: Taken together, oral administration of RPL-T at the rate of 191.1 mg/kg BW consistently increased CCK concentration, pancreatic α-amylase activity in duodenal fluids, and starch digestion rate in the small intestine and thus found to be beneficial.
Bioactive Sesterterpenoids from a Korean Sponge <i>Monanchora</i> sp.
Wang, Weihong,Mun, Bora,Lee, Yehee,Venkat Reddy, Mallepally,Park, Youngmin,Lee, Jihye,Kim, Hiyoung,Hahn, Dongyup,Chin, Jungwook,Ekins, Merrick,Nam, Sang-Jip,Kang, Heonjoong American Chemical Society and American Society of 2013 Journal of natural products Vol.76 No.2
<P>Chemical investigation of a Korean marine sponge, <I>Monanchora</I> sp., yielded nine new sesterterpenoids (<B>1</B>–<B>9</B>) along with phorbaketals A–C (<B>10</B>–<B>12</B>). The planar structures were established on the basis of NMR and MS analysis, and the absolute configurations of <B>1</B>–<B>9</B> were defined using the modified Mosher’s method and CD spectroscopic data analysis. Compounds <B>1</B>–<B>8</B>, designated as phorbaketals D–K, possess a spiroketal-modified benzopyran moiety such as phorbaketal A, and their structural variations are due to oxidation and/or reduction of the tricyclic core or the side chain. Compound <B>9</B>, designated as phorbin A, has a monocyclic structure and is proposed to be a possible biogenetic precursor of the phorbaketals. Compounds <B>1</B>–<B>9</B> were evaluated for cytotoxicity against four human cancer cell lines (A498, ACHN, MIA-paca, and PANC-1), and a few of them were found to exhibit cytotoxic activity.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jnprdf/2013/jnprdf.2013.76.issue-2/np300573m/production/images/medium/np-2012-00573m_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/np300573m'>ACS Electronic Supporting Info</A></P>
Lee, Jae-Wang,An, Jun Hyeong,Park, Sang-Hoon,Chong, Jong-Hyon,Kim, Gwang-Seok,Han, JeongJoon,Jung, Seunggon,Kook, Min-suk,Oh, Hee-Kyun,Ryu, Sun-Youl,Park, Hong-Ju Korean Association of Maxillofacial Plastic and Re 2016 Maxillofacial Plastic Reconstructive Surgery Vol.38 No.-
Background: The purpose of this retrospective study was to evaluate the clinical utility of an implant with a sandblasted, large-grit, acid-etched (SLA) surface and internal connection. Methods: Six patients who received dental implants in the Department of Oral and Maxillofacial Surgery, Chonnam National University Dental Hospital, were analyzed by factors influencing the success rate and marginal bone loss. Factors included patient's age, sex, implant installation site, whether bone graft was done, type of bone graft materials, approaching method if sinus lift was done, and the size of the fixture. In addition, the marginal bone loss was analyzed by using a radiograph. Results: All implants were successful, and the cumulative survival rate was 100 %. Average marginal bone loss of 6 months after the installation was 0.52 mm and 20 months after the functional loading was 1.06 mm. Total marginal bone resorption was 1.58 mm on average. There was no statistically significant difference in mesial and distal marginal bone loss. Conclusions: The short-term clinical success rate of the implant with an SLA surface and internal connection was satisfactory. Moreover, the marginal bone loss was also consistent with the implant success criteria.
Sang Min Lee,Soon Hee Kim,Ji Sung Kim,Joong San Wang,Sung Won Kim,Nyeon Jun Kim,Sook Hee Lee,Kyoung Ok Min 국제물리치료학회 2011 Journal of International Academy of Physical Ther Vol.2 No.2
The purpose of this study is to examine the changes in bone mineral density in long-stay patients of a geriatric hospital in relation to physical therapy, thereby providing basic data for preventing the onset or deterioration of osteoporosis in inpatients of geriatric hospitals or relevant facilities. The subjects of this study were 133 elderly patients who had been in H geriatric hospital in Yongin-si for more than four years. Their bone mineral density T-scores at admission and those after four years were measured to compare and analyze the changes in bone mineral density in relation to physical therapy. According to the results, the decrease in T-score for the group without physical therapy was greater by .40 than that for the group with physical therapy, indicating a statistically significant difference between the two groups(p<.01). Accordingly, physical therapy proved to be effective in preventing bone mineral density loss and maintaining the current density.
Embodiment of Spatially Arterial Pulse Diagnostic Apparatus using Array Multiple Hall Devices
Sang-Suk Lee,Gi-Wang Kim,Myung-Cheon Ahn,Young-Seok Park,Jong-Gu Choi,Sang-Dae Choi,Dal-Ho Park,Do-Guwn Hwang,Hyung-Rho Yoon 대한의용생체공학회 2007 의공학회지 Vol.28 No.6
The study relates to achievement and analysis of 3-dimensional spatial pulse wave archived by a spatially arterial pulse diagnostic apparatus (SAPDA), wherein a pulse sensing part array consists of multiple hall devices and is located over a skin contacting part which consists of a magnetic material. When a radially arterial pulse is transferred to the magnetic material, which is contacted skin that results in changes in a magnetic field of the lower part of the pulse sensing part array, the changes in a magnetic field can be detected by the commercial Hall semiconductor device of the pulse sensing part array. Finally, according to development of SAPDA, the 3-dimensionally arterial pulse waveform can be measured noninvasively by detecting the changes of the magnetic field.
Sang Min Lee,Joong San Wang,Sung Kyu Park,Hong Rae Kim,Jin Hee Ko,Yu Jung Oh,Hae Ran Yoon,Ji Sung Kim 국제물리치료학회 2012 Journal of International Academy of Physical Ther Vol.3 No.1
The aim of this study was to investigate the correlation among bone mineral density(BMD), body composition and body circumference on 20's college women in Hwaseong. A total of 86 subjects were measured with BMD and body composition and body circumference. To evaluate the correlation between BMD and body composition, bone density and body weight, body mass index(BMI), lean body mass, muscle mass, fat mass and body fat mass were compared. The results of this study, weight was considered the strong correlation with BMD than the height and BMI seems to be greater significance rather than the lumbar spine and femur BMD. In addition, the relationship between body composition and BMD, lean body mass, muscle mass, body fat mass were the most relevant factors and BMD. The relationship between BMD and body circumference that have been difficult because of not enough previous studies but somewhat the study showed that association.