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강원,경북지역 낙엽송 지위지수 적합성 평가에 관한 연구
이대성 ( Dae Sung Lee ),이의섭 ( Eui Sub Lee ),최종현 ( Jong Hyun Choi ),서영완 ( Yeong Wan Seo ),최정기 ( Jung Kee Choi1 ) 한국임학회 2014 산림과학 공동학술대회 논문집 Vol.2014 No.-
본 연구는 강원 및 경북지역의 낙엽송 지위지수의 적합도를 평가하기 위해 실시되었다. 연구자료는 강원 및 경북지역의 45개 낙엽송 인공림 표준지 조사로부터 우세목 1본을 선발하여 수간석 해를 통해 분석되었다. 산림청에서 개발된 지위지수표에 의거하여 본 연구대상지의 지위지수를 산정한 결과, 지위지수가 18 이하로 낮은 지역은 8곳(18%)이었으며, 지위지수가 20 이상으로 우수한 지역이 37곳(82%)으로서 대부분 지역에서 지위지수가 우세하였다. 지위지수곡선의 적합도를 평가하기위해 수간석해목의 과거에서 현재까지의 수고생장에 따른 지위지수의 변화를 도시한 결과, 초기 임령 때 지위지수와 차이가 없는 곳은 단지 8지역(18%)이었으며, 지위지수가 2∼4 m 차이가 발생한 지역이 19곳(42%), 6 m 이상 발생한 지역이 18곳(40%)으로 나타났다. This study was performed to evaluate the compatibility of site index for Larix kaempferi in Gangwon and Kyeongbuk regions. The data were collected and analyzed from stem alalysis of one dominant tree at each of 45 permanent plots installed in the regions. The result of site index estimation following the site index table developed by Korean Forest Service (KFS) showed that 8 plots (18%) were less than site index 18 and 37 plot (82%) greater than site index 20. Height growth changes of stemmed trees until present were illustrated in a graphic form to evaluate the compatibility of site index curve. The result showed that only 8 plots (18%) did not show any change of site index as trees age, 19 plots (42%) fluctuated in 2∼4m and 18 plots (40%) over 6m.
단상 유도형 동기 전동기(LSPM)의 특성해석에 관한 연구
정대성,김승주,이진헌,최재학,이주,Jung, Dae-Sung,Kim, Seung-Ju,Lee, Jin-Hun,Choi, Jae-Hak,Lee, Ju 한국조명전기설비학회 2007 조명·전기설비학회논문지 Vol.21 No.3
가전분야에서의 전동기 효율향상을 통한 에너지 소비절감 효과는 상당히 크지만 이를 위한 전동기의 설계/해석 기술 확보가 아직은 미흡한 상태이다. 이에 따라 유도형 동기전동기가 제안되었지만, 유도형 동기 전동기는 유도전동기와 통기전동기의 특성을 동시에 가지고 있어, 해석 및 설계가 쉽지 않다. 따라서 본 논문에서는 기존의 냉장고 압축기용 전동기인 단상유도전동기를 대처할 수 있는 단상 유도형 동기전동기를 이용해 파라미터에 따라 특성이 어떻게 바뀌는가에 대해 서술하였다. 그에 따라 단상유도전동기의 정상상태 특성을 해석하고 제안모델의 성능을 기존모델과 비교하고 실험하여 해석결과의 신뢰성을 검증한다. 또한 본 논문에서는 제안모델의 기존 고정자 형상이나 권선 스팩을 유지하고 회전자의 형상이나 구조만을 변경시켜 성능향상을 도모하였다. 해석방법으로는 전자장 수치 해석적인 방법으로 널리 이용되고 있는 유한요소법(Finite Element Method : FEM)을 이용하였다. Electric motor efficient improvement from home appliance field is important to the effect reducing the energy consumption. But the electric motor design/analysis technology is still insufficient. Specially the electric motor design/analysis technology of satisfying characteristic of both the induction motor and the synchronous motor such as Line start permanent motor. Therefore the electric motor design/analysis technology is urgently demanded reliability. This paper proposes the sing1e-phase line-start permanent magnet motor to develop the motor it with be able to alternate the sing-phase induction motor it is a refrigerator compressor motor. The sing-phase induction motor is analyzed in the steady state. And we have a certification test to compare our single-phase line-start permanent magnet motor with the sing-phase induction motor. In order to improve the performance, the stator of the single-phase line-start permanent magnet motor is same as the stator of the sing-phase induction motor and changes the rotor form and has the permanent magnet. It used the Finite Element Method(FEM) which is widely used with electronic-magnetic field numerical analysis method.
Cho, Eun Sook,Jin, Byung Rae,Sohn, Hung Dae,Choi, Kwang Ho,Kim, Soung Ryul,Kang, Seok Woo,Yun, Eun Young,Kim, Sang Hyun,Kim, Keun Young,Je, Yeon Ho,Kang, Seok Kwon 한국잠사학회 1998 한국잠사곤충학회지 Vol.40 No.2
To construct transfurmed Bm5 cells, Autographa californica nuclear polyhedrosis virus(AcNPV) IE1 gene, an immediate early viral gene was firstly used in this study. AcNPV IE1 gene, which shares on 95.3% nucleotide sequence homology with Bombyx mori nuclear polyhedrosis virus (BmNPV) IE1 gene, was isolated and cloned into pBluescript. Neomycin gene from pKO-neo was inserted under the control of the IE1 promoter to yield pAcIE1-neo. The plasmid pAcIE1-neo was transfected into Bm5 or Sf9 cells, and neomycin-resistant cells were selected in TC100 medium containing 10% fetal bovine serum (FBS) and 1 mg/ml G4l8 for two weeks. Individual clones were picked and each was amplified for further characterization. The genomic DNA from neomycin-resistant cells was isolated and characterized by PCR using AcNPV IEI gene-specific primers and by Southern blot analysis using neomycin gene probe. We concluded that AcNPV IE1 gene was functional in B. mori-derived Bm5 cells as well as Spodoptera fugjprrda-derived Sf9 cells to produce stably-transformed insect cells
Transduced Tat-α-Synuclein Protects against Oxidative Stress In vitro and In vivo
Choi, Hee-Soon,Lee, Sun-Hwa,Kim, So-Young,An, Jae-Jin,Hwang, Seok-Il,Kim, Dae-Won,Yoo, Ki-Yeon,Won, Moo-Ho,Kang, Tae-Cheon,Kwon, Hyung-Joo,Kang, Jung-Hoon,Cho, Sung-Woo,Kwon, Oh-Shin,Choi, Jin-Hi,Park Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.3
Parkinson's disease (PD) is a common neurodegenerative disorder and is characterized by the progressive loss of dopaminergic neurons in the substantia nigra. Although many studies showed that the aggregation of $\alpha$-synuclein might be involved in the pathogenesis of PD, its protective properties against oxidative stress remain to be elucidated. In this study, human wild type and mutant $\alpha$-synuclein genes were fused with a gene fragment encoding the nine amino acid trans activator of transcription (Tat) protein transduction domain of HIV-l in a bacterial expression vector to produce a genetic in-frame WT Tat-$\alpha$-synuclein (wild type) and mutant Tat-a-synucleins (mutants; A30P and A53T), respectively, and we investigated the protective effects of wild type and mutant Tat-$\alpha$-synucleins in vitro and in vivo. WT Tat-$\alpha$-synuclein rapidly transduced into an astrocyte cells and protected the cells against paraquat induced cell death. However, mutant Tat-$\alpha$-synucleins did not protect at all. In the mice models exposed to the herbicide paraquat, the WT Tat-$\alpha$-synuclein completely protected against dopaminergic neuronal cell death, whereas mutants failed in protecting against oxidative stress. We found that these protective effects were characterized by increasing the expression level of heat shock protein 70 (HSP70) in the neuronal cells and this expression level was dependent on the concentration of transduced WT Tat-$\alpha$-synuclein. These results suggest that transduced Tat-$\alpha$-synuclein might protect cell death from oxidative stress by increasing the expression level of HSP70 in vitro and in vivo and this may be of potential therapeutic benefit in the pathogenesis of PD.
Cloning and Expression of Partial Japanese Flounder (Paralichthys olivaceus) IgD
Choi, Dae-Han,Jang, Han-Na,Ha, Dae-Mang,Kim, Jae-Wha,Oh, Chan-Ho,Choi, Sang-Hoon Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.4
The cDNA sequence of the Japanese flounder (Paralychthys olivaceus) IgD has been previously reported (GenBank accession no. AB052658) and this was followed by the detection of IgD mRNA expression in some flounder organ tissues. However, it has not been determined whether the flounder IgD gene is virtually expressed into IgD protein. To characterize the flounder immunoglobulins utilized in elucidating the mechanism, evolution and diversity of the flounder immune system, antibodies specific to IgD and IgM were necessary. In the present study, partial flounder recombinant IgD (rIgD), IgM (rIgM) and the conserved regions of IgD and IgM (rCIg) were produced by cloning the cDNA sequence using isotype specific primers which were designed to produce unique fragments of IgD and IgM specific amino acid sequences. The production of recombinant Igs was ascertained by SDS-gel electrophoresis and immunoblot analysis using anti-T7$\cdot}$Taq antibody. The produced recombinant Igs were purified using affinity columns, and used as immunogens. Antibodies specific to the isotype of flounder Igs were generated by immunizing rabbits with rfIgs and the antibodies produced were identified by enzyme-linked immunosorbent assay (ELISA) and immunoblotting. Specificities of the generated antibodies were evaluated by testing cross-reactivity between recombinant IgM and IgD. By ELISA, rabbit antibodies against the rfIgD fragment (anti-rfIgD) failed to recognize any kind of flounder serum Igs, whereas respective antibodies against rfCIg (anti-rfCIg) and rfIgM fragments (anti-rfIgM) reacted with serum Igs. Likewise, in immunoblot assays, though anti-rfIgD did not, both anti-rfCIg and anti-rfIgM bound with the ~85 kd flounder IgM heavy chain. By flow cytometry analysis, anti-rfCIg, anti-rfIgD and anti-rfIgM reacted with 6%, 3% and 6.5% of cells, respectively, suggesting that flounder IgD is not secreted in serum but expressed on flounder B-like cell surfaces as in mammals. Antibodies produced against recombinant flounder Igs could be used to develop sandwich assay systems for detecting flounder Igs and for further investigating the flounder immune system.
Non-isolated Boost Charger for the Li-Ion Batteries Suitable for Fuel Cell Powered Laptop Computers
Sang, Nguyen Van,Choi, Woojin,Kim, Dae-Wook The Korean Institute of Power Electronics 2013 JOURNAL OF POWER ELECTRONICS Vol.13 No.1
The conventional non-isolated boost converter has some drawbacks such as poor dynamic performance and a discontinuous output current, which make it unsuitable for battery charging applications. In spite of its compactness and lightness, it is not preferred as a charger of portable electronic devices. In this paper, a non-isolated boost converter topology for Li-ion batteries suitable for fuel cell powered laptop computers is proposed and analyzed. The proposed converter has an additional inductor at the output to make a continuous output current. This feature makes it suitable for charger applications by eliminating the disadvantages of the conventional non-isolated boost converter mentioned above. A prototype of the proposed converter is built for the Li-ion battery charger of a laptop computer to prove the validity and advantages of the proposed topology.
Choi, Hee-Don,Han, Jeong-Jun,Yang, Ji-Hee,Lee, Sang-Hoon,Kim, Yun-Sook,Chung, Guk-Hoon,Hahm, Dae-Hyun The Korean Society for Applied Biological Chemistr 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.2
Effect of supplementation of phosphatidylserine (PS), enzymatically synthesized from soy lecithin, on skin moisture and wrinkle in animal and human was investigated. Skin moisture content of hairless mice was significantly decreased and skin thickness and wrinkle were significantly increased by UV irradiation, whereas PS-supplemented groups showed reduced wrinkle depth and much less wrinkle area unlike UV control (UV/Con) group. The collagen content in PS-supplemented groups increased compared with that in UV/Con group. A placebo-controlled double-blind intake study of soft capsules containing PS (300 mg/day) was performed on 63 subjects who had normal healthy skin for 12 weeks. Dermatologist's visual assessment and image analysis of replicas showed that wrinkle near eye was improved by PS-supplementation. Upon measurement of moisture content in the skin, PS supplementation increased the moisture content in the skin. These findings showed that PS supplementation was effective in moisturizing and improving wrinkle of the skin in both animal and human; thus, PS could be used as an effective skin food ingredient.