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An Sunho,Vo Tam Thuy Lu,Son Taekwon,Choi Hoon,Kim Jinyoung,Lee Juyeon,Kim Byung Hoon,Choe Misun,Ha Eunyoung,Surh Young-Joon,Kim Kyu-Won,Seo Ji Hae 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-
Human sterile α motif and HD domain-containing protein 1 (SAMHD1) has deoxyribonucleoside triphosphohydrolase (dNTPase) activity that allows it to defend against human immunodeficiency virus type I (HIV-1) infections and regulate the cell cycle. Although SAMHD1 mutations have been identified in various cancer types, their role in cancer is unclear. Here, we aimed to investigate the oncogenic role of SAMHD1 in human clear cell renal cell carcinoma (ccRCC), particularly as a core molecule promoting cancer cell migration. We found that SAMHD1 participated in endocytosis and lamellipodia formation. Mechanistically, SAMHD1 contributed to the formation of the endosomal complex by binding to cortactin. Thereafter, SAMHD1-stimulated endosomal focal adhesion kinase (FAK) signaling activated Rac1, which promoted lamellipodia formation on the plasma membrane and enhanced the motility of ccRCC cells. Finally, we observed a strong correlation between SAMHD1 expression and the activation of FAK and cortactin in tumor tissues obtained from patients with ccRCC. In brief, these findings reveal that SAMHD1 is an oncogene that plays a pivotal role in ccRCC cell migration through the endosomal FAK-Rac1 signaling pathway.
( Sunho Park ),( Hyun-ha Park ),( Yonghyun Gwon ),( Sujin Kim ),( Daun Kim ),( Hoon Eui Jeong ),( Jangho Kim ) 한국농업기계학회 2019 한국농업기계학회 학술발표논문집 Vol.24 No.2
Nanoengineering offers great opportunities for advanced applications in agricultural and biological fields. Here, we proposed a bio-instructive nanospike patch with the precisely controlled sizes using a sophisticated nanoengineering (e.g., capillary force lithography). To investigate the effect of a developed nanospike, the various types of cells including the mammalian cells and bacterial were cultured. Interestingly, the nanospike can both promote the behaviors including proliferation, differentiation, and cytokine excretion of the mammalian cells and prevent the bacterial biofilm formation by the designed feature sized of the nanospike. More specifically, the mammalian cells with relatively large size were received the proper structural stimulation from the nanopske, while the bacterial membrane were damaged by the structure of the nanospike. Our finding reveal the importance of the nanoscale sizes of the spikes to regulate the behaviors of cells, and the proposed nanospike could be used as new bio-instructive platforms such as food packaging and cell therapy platforms in agricultural and biological fields.
Sunho Choe,이준기,Jeeyoo Lee,Daehee Kang,Jong-Koo Lee,신애선 대한예방의학회 2019 Journal of Preventive Medicine and Public Health Vol.52 No.6
Objectives: Self-reported disease history is often used in epidemiological studies. In this study, we acquired the hospital records of subjects who self-reported stroke or myocardial infarction (MI) and evaluated the validity of the participants’ self-reported disease history. We also determined the level of agreement between specialists and non-specialists. Methods: Among the participants in the Health Examinees study, 1488 subjects self-reported stroke or MI during 2012-2017, and medical records were acquired for the 429 subjects (28.8%) who agreed to share their medical information. Each record was independently assigned to 2 medical doctors for review. The records were classified as ‘definite,’ ‘possible,’ or ‘not’ stroke or MI. If the doctors did not agree, a third doctor made the final decision. The positive predictive value (PPV) of self-reporting was calculated with the doctors’ review as the gold standard. Kappa statistics were used to compare the results between general doctors and neurologists or cardiologists. Results: Medical records from 208 patients with self-reported stroke and 221 patients with self-reported MI were reviewed. The PPV of self-reported disease history was 51.4% for stroke and 32.6% for MI. If cases classified as ‘possible’ were counted as positive diagnoses, the PPV was 59.1% for stroke and 33.5% for MI. Kappa statistics showed moderate levels of agreement between specialists and non-specialists for both stroke and MI. Conclusions: The validity of self-reported disease was lower than expected, especially in those who reported having been diagnosed with MI. Proper consideration is needed when using these self-reported data in further studies.
Engineering Eggshell Membranes for Manipulation of Nanotopographically-defined Functional Platforms
( Sunho Park ),( Daun Kim ),( Woochan Kim ),( Dohyeon Lee ),( Sungmin Park ),( Sujin Kim ),( Jangho Kim ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1
Eggshell membrane (ESM), one of the agricultural bioresources, is being recognized as emerging engineering platforms in various fields including agriculture and biological engineering. Eggshell membrane is the protein-rich membrane between the eggshell and egg white, having interesting characteristics such as structural, chemical, and physical properties. However, the raw ESM has many limitations (e.g., low electric capability and mechanical property), which raised some problems for its wide use as commercial engineering platforms. Here, we present new ESM-based engineering platforms based on nanotechnology. To develop the nanotopographically-enhanced ESM platforms, we used graphene, one of the carbon-based nanomaterials, that provides unique nanotopographically-defined cues to the raw ESM. The graphene-ESM hybrid platforms showed hierarchically micro- and nanoscale structures (i.g., microfibers from ESM and nanosheets from graphene). It is also found that the graphene-ESM hybrid platforms could enhance the electrical and mechanical property compared to the raw ESM. Our approach can enable the design and manipulation of ESM-based functional engineering platforms for various applications such as agricultural, biological, environmental, and biomedical platforms (e.g., biomaterials, electric devices, sensors, air purification systems).
Full Scale Performance Prediction of an Energy Saving Device by Using Computational Fluid Dynamics
Sunho Park,Gwangho Oh,Shin Hyung Rhee 한국전산유체공학회 2014 한국전산유체공학회 학술대회논문집 Vol.2014 No.10
Recently interest on energy saving devices (ESD’s) has increased with the enforcement of the energy efficiency design index (EEDI) verification proposed by the international maritime organization (IMO). Extension of propulsive performance results from model to full scale ships plays an important role in verification of ships with ESD’s. The present study proposed a reliable and efficient propulsive performance prediction method for full scale ships with ESD’s. The propulsive performance prediction in full scale KVLCC2 with pre-swirl stator (PSS) was conducted by the proposed method. Its results were then compared with those by the existing extension methods and by full scale CFD computations. From the results, it was confirmed that the proposed method could extend the model scale results to full scale ones with ESD’s performance improvement effects. Unlike the existing methods, it takes into account of ESD’s and consumes much less computational resources and time than full scale CFD computations.