Melatonin Plays a Role as a Mediator of Nocturnal Pain in Patients with Shoulder Disorders

Ha, Eunyoung,Lho, Yun-Mee,Seo, Hyuk-Jun,Cho, Chul-Hyun Journal of BoneJoint Surgery, Inc. 2014 Journal of bone and joint surgery Vol.96 No.13

<P><B>Background:</B></P><P>Nocturnal pain is commonly observed in patients with shoulder disorders such as a rotator cuff tear or frozen shoulder. This study was conducted to explore the possibility that melatonin plays a role as a mediator of nocturnal pain in patients with a rotator cuff tear or frozen shoulder.</P><P><B>Methods:</B></P><P>Subacromial bursa and joint capsule samples were collected from sixty-three patients: twenty-one patients with a rotator cuff tear, twenty-two with frozen shoulder, and twenty with shoulder instability (control group). The expression of melatonin receptor 1A (MTNR1A) and 1B (MTNR1B) and of acid-sensing ion channel 3 (ASIC3) in the subacromial bursa and the joint capsule were determined by real-time reverse transcription-polymerase chain reaction (RT-PCR) analysis. The protein level of ASIC3 was measured by immunoblot analysis. To determine the effect of melatonin as a pain mediator, an in vitro study with use of primary cultured fibroblast-like synoviocytes was performed by semiquantitative RT-PCR analysis, immunoblot analysis, and enzyme-linked immunosorbent assay (ELISA).</P><P><B>Results:</B></P><P>MTNR1A, MTNR1B, and ASIC3 expression was significantly increased in both the rotator cuff tear and frozen shoulder groups compared with the control group of patients with shoulder instability. Interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) significantly stimulated the expression of MTNR1A and MTNR1B in primary cultured fibroblast-like synoviocytes treated with proinflammatory cytokines. Melatonin treatment at a physiological concentration (10 nM) induced ASIC3 expression and IL-6 production. Treatment with luzindole, a melatonin-receptor antagonist, reversed melatonin-stimulated ASIC3 expression and IL-6 production.</P><P><B>Conclusions:</B></P><P>Our study suggests that melatonin may play a role as a mediator of nocturnal pain with a rotator cuff tear or frozen shoulder, and this effect may be mediated via melatonin receptors.</P><P><B>Clinical Relevance:</B></P><P>Melatonin may be a therapeutic target of chronotherapy.</P>

Microarray analysis of transcription factor gene expression in melatonin-treated human peripheral blood mononuclear cells

Ha, Eunyoung,Han, Earl,Park, Hae Jeong,Kim, Hak-Jae,Hong, Mee Suk,Hong, Seung-Jae,Yoon, Kyung Sik,Kang, Insug,Cho, Yong Hoo,Chung, Joo-Ho,Yim, Sung-Vin,Baik, Hyung Hwan Blackwell Publishing Ltd 2006 Journal of pineal research Vol.40 No.4

<P>Abstract: </P><P>The existence of specific melatonin-binding sites in lymphoid cells led to the discovery of signal transduction pathway for melatonin in human lymphocytes and immunomodulatory role of melatonin in immune cells. In recent years, transcriptional regulation of melatonin on various transcription factors has been demonstrated. Therefore, this study was designed to assess by cDNA microarray analysis the regulatory effects of melatonin on transcription factors in human peripheral blood mononuclear cells (PBMCs). Forty-six genes were upregulated and 23 were downregulated more than twofold in melatonin-treated PBMCs. Of the more than twofold upregulated transcription factor genes, homeo box A4 (<I>HOXA4</I>), forkhead box O1A (<I>FOXO1A</I>), transcription elongation factor B (SIII), polypeptide 3 (<I>TCEB3</I>), and peroxisome proliferative activated receptor delta (<I>PPARD</I>) were identified. Of the more than twofold downregulated genes, PHD finger protein 15 (<I>PHF15</I>) and zinc finger protein 33a (<I>ZNF33A</I>) were identified. In summary, identification of these genes by cDNA microarray analysis in response to melatonin administration may provide a foundation for further studies on the function of melatonin in human PBMCs.</P>

Key Performance Indicators (KPIs) for FM services

Eunyoung Shin,Yujin Kim,Jun Ha Kim 한국주거학회 2015 한국주거학회 국제학술대회논문집 Vol.2015 No.4

Functional Insights of Ceramides in Epidermis

( Eunyoung Lee ),( Yujia Han ),( Jeayoung Shin ),( Yeon Kyung Kim ),( Jeongjoo Pyo ),( Sungjin Ahn ),( Jaehyoun Ha ),( Minsoo Noh ) 한국피부장벽학회 2018 한국피부장벽학회지 Vol.20 No.1

Intercellular lipids of stratum corneum mainly consist of ceramides, free fatty acids, and cholesterol. Ceramides are synthesized in endoplasmic reticulum and transported to the lamellar bodies which provide intercellular lipids in stratum corneum during epidermal differentiation. The lipid mass of ceramides is approximately 50% of the intercellular lipid contents in stratum corneum. The decrease in ceramide levels significantly impairs the integrity of skin permeability barrier and results in the increase in transepidermal water loss. The content and quantity of ceramides can directly affect the lamellar layer rigidity of stratum corneum lipid matrix and genetic mutations or polymorphisms of ceramide metabolic enzymes are associated with the disrupted skin barrier functions. In addition to the structural role in skin permeability barrier, ceramides are important in the regulation of cell biology functions such as ultraviolet B irradiation-induced cellular apoptosis, inflammation-related autophagy, and the proliferation and differentiation of epidermal keratinocytes. Besides skin, ceramides and their metabolites are also interested in other tissues because ceramides are associated with various human diseases like diabetes and cancers. Notably, skin microbiota can affect the ceramide metabolism and change the content of ceramides and their metabolites in stratum corneum lipid matrix. Sphingosine choline phosphotransferase and sphingomyelin deacylase may increase sphingosylphosphorylcholine (SPC), a ceramide metabolite increased in the stratum corneum of some atopic patient population. The genes of these metabolic enzymes responsible for the increase in SPC have not been discovered in human cell studies. It is possible that the abnormal SPC production in atopic skin may be associated with microorganism-derived ceramide metabolic enzymes. Therefore, research on skin microbiota should be directed toward the elucidation of microbiota-associated ceramide metabolic enzymes to understand the ceramide homeostasis in human epidermis.

Cyanate attenuates insulin secretion in cultured pancreatic β cells.

D. A. Spandidos 2012 MOLECULAR MEDICINE REPORTS Vol.5 No.6

<P>The vast majority of long-term complications in transplanted patients are associated with cardiovascular disease. Previously, an alternative and dominant mechanism for cyanate formation in atherosclerotic lesions has been discovered. This study was designed to determine the effect of cyanate on insulin secretion in cultured pancreatic β cells (INS-1 cells). The cytotoxicity of cyanate was determined by 3-(4,5-Desethyithiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Insulin secretion was measured by ELISA in cyanate-treated INS-1 cells. Reactive oxygen species (ROS) generation was also determined by measuring the fluorescent oxidized product of 2,7-dichlorefluorescein in cyanate-treated INS-1 cells. FACS analysis was carried out to determine the effect of cyanate on the apoptosis of INS-1 cells. Firstly, we found that cyanate, within concentration ranges in which no cytotoxic effect was observed (0.01, 0.1 and 1.0 mM), decreased insulin secretion dose-dependently in both non-glucose-stimulated and glucose-stimulated INS-1 cells. Cyanate at a 1.0 mM concentration inhibited insulin secretion by more than 50% in non-glucose-stimulated cells and glucose (5 and 10 mM)-stimulated cells. Cyanate, however, did not affect ROS generation. Furthermore, no pro- or anti-apoptotic effect was observed in cyanate-treated INS-1 cells. The results in this study suggest the possible inhibitory effect of cyanate on insulin secretion in INS-1 pancreatic β cells. The inhibitory effect was not mediated either by ROS generation or by apoptosis. Further studies to determine the underlying mechanisms will be of benefit.</P>

Effect of Monounsaturated Fatty Acid Enriched Peanut Consumption on Plasma Lipid Profile in a High Fat Diet-Induced Mouse Model

Eunyoung Oh,Ji Myung Choi,Bo Gyeong Seol,Suk-Bok P,Myong Hee,Jung In Kim,Sungup Kim,Doyeon Kwak,Tae Joung Ha,Eun Ju Cho 한국식품영양과학회 2018 한국식품영양과학회 학술대회발표집 Vol.2018 No.10

Generation of 3-D Spheroids by Hanging Drop Method

Yuna Ha,KeunJung Kim,EunYoung Kim,Li Xiaoxia,Jihye Lee,KangSun Park,YounBae Park,Maria Kim,Kilwoo Han,Jeong Yu,DongSoo Lee,MinKyu Kim 한국동물생명공학회(구 한국동물번식학회) 2012 발생공학 국제심포지엄 및 학술대회 Vol.2012 No.1

Penalized variable selection for accelerated failure time models

Park, Eunyoung,Ha, Il Do The Korean Statistical Society 2018 Communications for statistical applications and me Vol.25 No.6

The accelerated failure time (AFT) model is a linear model under the log-transformation of survival time that has been introduced as a useful alternative to the proportional hazards (PH) model. In this paper we propose variable-selection procedures of fixed effects in a parametric AFT model using penalized likelihood approaches. We use three popular penalty functions, least absolute shrinkage and selection operator (LASSO), adaptive LASSO and smoothly clipped absolute deviation (SCAD). With these procedures we can select important variables and estimate the fixed effects at the same time. The performance of the proposed method is evaluated using simulation studies, including the investigation of impact of misspecifying the assumed distribution. The proposed method is illustrated with a primary biliary cirrhosis (PBC) data set.

High Prevalence of Listeria monocytogenes in Smoked Duck: Antibiotic and Heat Resistance, Virulence, and Genetics of the Isolates

Park, Eunyoung,Ha, Jimyeong,Oh, Hyemin,Kim, Sejeong,Choi, Yukyung,Lee, Yewon,Kim, Yujin,Seo, Yeongeun,Kang, Joohyun,Yoon, Yohan Korean Society for Food Science of Animal Resource 2021 한국축산식품학회지 Vol.41 No.2

This study aimed at determining the genetic and virulence characteristics of the Listeria monocytogenes from smoked ducks. L. monocytogenes was isolated by plating, and the isolated colonies were identified by PCR. All the obtained seven L. monocytogenes isolates possessed the virulence genes (inlA, inlB, plcB, and hlyA) and a 385 bp actA amplicon. The L. monocytogenes isolates (SMFM2018 SD 1-1, SMFM 2018 SD 4-1, SMFM 2018 SD 4-2, SMFM 2018 SD 5-2, SMFM 2018 SD 5-3, SMFM 2018 SD 6-2, and SMFM 2018 SD 7-1) were inoculated in tryptic soy broth (TSB) containing 0.6% yeast extract at 60℃, followed by cell counting on tryptic soy agar (TSA) containing 0.6% yeast extract at 0, 2, 5, 8, and 10 min. We identified five heat resistant isolates compared to the standard strain (L. monocytogenes ATCC13932), among which three exhibited the serotype 1/2b and D-values of 5.41, 6.48, and 6.71, respectively at 60℃. The optical densities of the cultures were regulated to a 0.5 McFarland standard to assess resistance against nine antibiotics after an incubation at 30℃ for 24 h. All isolates were penicillin G resistant, possessing the virulence genes (inlA, inlB, plcB, and hlyA) and the 385-bp actA amplicon, moreover, three isolates showed clindamycin resistance. In conclusion, this study allowed us to characterize L. monocytogenes isolates from smoked ducks, exhibiting clindamycin and penicillin G resistance, along with the 385-bp actA amplicon, representing higher invasion efficiency than the 268-bp actA, and the higher heat resistance serotype 1/2b.

Estimation of Oil Yield of Perilla by Seed Characteristics and Crude Fat Content

Oh, Eunyoung,Lee, Myoung Hee,Kim, Jung In,Kim, Sungup,Pae, Suk-Bok,Ha, Tae Joung The Korean Society of Crop Science 2018 한국작물학회지 Vol.63 No.2

Perilla (Perilla frutescens var.frutescens) is an annual plant of the Lamiaceae family, mainly grown for obtaining oil by press extraction after roasting the seeds. Oil yield is one of its important traits, but evaluating this yield is time-consuming, requires many seeds, and is hard to adjust to pedigrees in a breeding field. The objective of this study was to develop a method for selecting high-oil-yield lines in a breeding population without oil extraction. Twenty-three perilla cultivars were used for evaluating the oil yield and seed traits such as seed hardness, seed coat thickness, seed coat proportion and crude fat. After evaluation of the seed traits of 23 perilla cultivars, the ranges of oil yields, seed hardness, seed coat thickness, seed coat proportion, 100-seed weight, and crude fat were 24.68-38.75%, 157-1166 gf, $24-399{\mu}m$, 15.4-41.5%, 2.79-6.69 g, and 33.0-47.8%, respectively. In an analysis of correlation coefficients, the oil yield negatively correlated with seed length, seed width, the proportion of seed coat, seed hardness, and 1000-seed weight, but positively correlated with crude fat content. It was observed that as the seed coat proportion increased, the seed coat thickness, hardness, and 1000-seed weight also increased. Multiple linear regression (MLR) was employed to find major variables affecting the oil yield. Among the variables, traits crude fat content and seed coat proportion were assumed to be indirect parameters for estimating the potential oil yield, with respect to a significant positive correlation with the observed oil yield ($R^2=0.791$). Using these two parameters, an equation was derived to predict the oil yield. The results of this study show that various seed traits in 23 perilla cultivars positively or negatively correlated with the oil yield. In particular, crude fat and the seed coat proportion can be used for predicting the oil yield with the newly developed equation, and this approach will improve the efficiency of selecting prominent lines for the oil yield.