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Characterization of Echinostoma cinetorchis endoribonuclease, RNase H
Sung-Bin Lim,Seok Ho Cha,Seung Jegal,Hojong Jun,Seo Hye Park,Bo-Young Jeon,Jhang Ho Pak,Young Yil Bakh,Tong-Soo Kim,Hyeong-Woo Lee 대한기생충학열대의학회 2017 The Korean Journal of Parasitology Vol.55 No.4
Echinostoma cinetorchis is an oriental intestinal fluke causing significant pathological damage to the small intestine. The aim of this study was to determine a full-length cDNA sequence of E. cinetorchis endoribonuclease (RNase H; EcRNH) and to elucidate its molecular biological characters. EcRNH consisted of 308 amino acids and showed low similarity to endoribonucleases of other parasites (<40%). EcRNH had an active site centered on a putative DDEED motif instead of DEDD conserved in other species. A recombinant EcRNH produced as a soluble form in Escherichia coli showed enzymatic activity to cleave the 3-O-P bond of RNA in a DNA-RNA duplex, producing 3-hydroxyl and 5-phosphate. These findings may contribute to develop antisense oligonucleotides which could damage echinostomes and other flukes.
( Sung Ha Lim ),( Solam Lee ),( Young Bin Lee ),( Chung Hyeok Lee ),( Jong Won Lee ),( Sang Hoon Lee ),( Ju Yeong Lee ),( Joung Soo Kim ),( Mi Youn Park ),( Sang Baek Koh ),( Eung Ho Choi ) 대한피부과학회 2020 대한피부과학회 학술발표대회집 Vol.72 No.1
Background: Whether having a tattoo increases the risk of transfusion transmitted diseases (TTDs) is controversial. Objectives: To identify prevalence and risk of TTDs in people with tattoos as compared with the general population. Methods: A systematic review of the studies published before September 2019 was performed by using the Medline, Embase, and Web of Science databases. Observational studies on hepatitis C virus (HCV), hepatitis B virus (HBV), human immunodeficiency virus (HIV), and syphilis infections in people with and without tattoos were included. Studies that reported disease status without serological confirmation were excluded. Results: A total of 115 studies were quantitatively analyzed. HCV (odd ratio [OR], 2.94; 95% confidence interval [CI], 2.52-3.44), HBV (OR, 1.53; 95% CI, 1.34-1.76), and HIV infections (OR, 3.42; 95% CI, 2.16-5.41) were more prevalent in the tattooed population. In subgroup analyses, after the adjustment of possible publication bias, the prevalence of HCV infection was significantly elevated in the community-dwelling, hospital patient, intravenous (IV) drug user, and prisoner groups. IV drug users and prisoners showed high prevalence rates of HBV infection. The prevalence of HIV infection was significantly increased in the prisoner group. Conclusion: Having a tattoo is associated with an increased prevalence of TTDs. Our approach clarifies in depth and supports a guideline for TTD screening in the tattooed population.
Prognostic factors associated with the success rates of posterior orthodontic miniscrew implants
Sung-Bin Hong,Budi Kusnoto,Eun-Jeong Kim,Ellen A BeGole,Hyeon-Shik Hwang,Hoi-Jeong Lim 대한치과교정학회 2016 대한치과교정학회지 Vol.46 No.2
Objective: To systematically review previous studies and to assess, via a subgroup meta-analysis, the combined odds ratio (OR) of prognostic factors affecting the success of miniscrew implants (MIs) inserted into the buccal posterior region. Methods: Three electronic searches that were limited to articles on clinical human studies using MIs that were published in English prior to March 2015 were conducted. The outcome measure was the success of MIs. Patient factors included age, sex, and jaw of insertion (maxilla vs. mandible), while the MI factors included length and diameter. A meta-analysis was performed on 17 individual studies. The quality of each study was assessed for non-randomized studies and quantified using the Newcastle-Ottawa Scale. The meta-analysis outcome was a combined OR. Subgroup and sensitivity analyses based on the study design, study quality, and sample size of miniscrews implanted were performed. Results: Significantly higher success rates were revealed for MIs inserted in the maxilla, for patients ≥ 20 years of age, and for long MIs (≥ 8 mm) and MIs with a large diameter (> 1.4 mm). All subgroups acquired homogeneity, and the combined OR of the prospective studies (OR, 3.67; 95% confidence interval [CI], 2.10−6.44) was significantly higher in the maxilla than that in the retrospective studies (OR, 2.10; 95% CI, 1.60−2.74). Conclusions: When a treatment plan is made, these risk factors, i.e. jaw of insertion, age, MI length, and MI diameter, should be taken into account, while sex is not critical to the success of MIs.
Sang-a Park,Gihwan Byeon,Jin Hyeong Jhoo,Hyung-Chun Kim,Myoung-Nam Lim,Jae-Won Jang,Jong Bin Bae,Ji Won Han,Tae Hui Kim,Kyung Phil Kwak,Bong Jo Kim,Shin Gyeom Kim,Jeong Lan Kim,Seok Woo Moon,Joon Hyuk 대한정신약물학회 2023 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.21 No.4
Objective: To investigate the relationship between reduced glutathione (GSH), a key molecule of the antioxidant defense system in the blood, and glutathione reductase (GR), which reduces oxidized glutathione (glutathione disulfide [GSSG]) to GSH and maintains the redox balance, with the prevalence of Alzheimer’s dementia and cognitive decline. Methods: In all, 20 participants with Alzheimer’s dementia who completed the third follow-up clinical evaluation over 6 years were selected, and 20 participants with normal cognition were selected after age and sex matching. The GSH and GR concentrations were the independent variables. Clinical diagnosis and neurocognitive test scores were the dependent variables indicating cognitive status. Results: The higher the level of GR, the greater the possibility of having normal cognition than of developing Alzheimer’s dementia. Additionally, the higher the level of GR, the higher the neurocognitive test scores. However, this association was not significant for GSH. After 6 years, the conversion rate from normal cognition to cognitive impairment was significantly higher in the lower 50th percentile of the GR group than in the upper 50th percentile. Conclusion: The higher the GR, the lower the prevalence of Alzheimer’s dementia and incidence of cognitive impairment and the higher the cognitive test scores. Therefore, GR is a potential protective biomarker against Alzheimer’s dementia and cognitive decline.
Lim, Ji-Young,Ryu, Da-Bin,Lee, Sung-Eun,Park, Gyeongsin,Choi, Eun Young,Min, Chang-Ki Korean Society for Molecular and Cellular Biology 2015 Molecules and cells Vol.38 No.11
Despite the presence of toll like receptor (TLR) expression in conventional $TCR{\alpha}{\beta}$ T cells, the direct role of TLR signaling via myeloid differentiation factor 88 (MyD88) within T lymphocytes on graft-versus-host disease (GVHD) and graft-versus-leukemia (GVL) effect after allogeneic stem cell transplantation (allo-SCT) remains unknown. In the allo-SCT model of C57BL/6 ($H-2^b$) ${\rightarrow}$ B6D2F1 ($H-2^{b/d}$), recipients received transplants of wild type (WT) T-cell-depleted (TCD) bone marrow (BM) and splenic T cells from either WT or MyD88 deficient (MyD88KO) donors. Host-type ($H-2^d$) P815 mastocytoma or L1210 leukemia cells were injected either subcutaneously or intravenously to generate a GVHD/GVL model. Allogeneic recipients of MyD88KO T cells demonstrated a greater tumor growth without attenuation of GVHD severity. Moreover, GVHD-induced GVL effect, caused by increasing the conditioning intensity was also not observed in the recipients of MyD88KO T cells. In vitro, the absence of MyD88 in T cells resulted in defective cytolytic activity to tumor targets with reduced ability to produce IFN-${\gamma}$ or granzyme B, which are known to critical for the GVL effect. However, donor T cell expansion with effector and memory T-cell differentiation were more enhanced in GVHD hosts of MyD88KO T cells. Recipients of MyD88KO T cells experienced greater expansion of Foxp3- and IL4-expressing T cells with reduced INF-${\gamma}$ producing T cells in the spleen and tumor-draining lymph nodes early after transplantation. Taken together, these results highlight a differential role for MyD88 deficiency on donor T-cells, with decreased GVL effect without attenuation of the GVHD severity after experimental allo-SCT.
Protective Effect of Morin on the Imipenem-induced Nephrotoxicity in Rabbits
Lim, Sung-Chul,Im, Young-Bin,Bae, Chun-Sik,Han, Song-Iy,Kim, Se-Eun,Han, Hyo-Kyung 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.8
The present study investigated the protective effect of morin, a natural flavonoid, on the imipenem-induced nephrotoxicity in rabbits. Nephrotoxicity of imipenem was examined after the intravenous administrations of imipenem (200 mg/kg) to rabbits in the presence and the absence of morin (12, 25, 50 mg/kg, p.o.). Cytotoxicity of imipenem was also examined in the presence and the absence of morin ($100\;{\mu}M$) by using MDCK cells overexpressing human organic anion transporter 1 and 3 (MDCK/hOAT1 or MDCK/hOAT3). Intravenous dosing of imipenem alone induced severe proximal tubular necrosis in rabbits, however, the concurrent use of morin (25 or 50 mg/kg, p.o.) significantly suppressed the histopathological damage in the kidney induced by imipenem. While imipenem was not cytotoxic in MDCK/hOAT1 cells over the tested concentrations up to 10 mM, it showed significant cellular toxicity with $CC_{50}$ of 0.77 mM in MDCK/hOAT3 cells, implying that OAT3 may involve more actively in the imipeneminduced nephrotoxicity. In addition, the cellular toxicity of imipenem decreased by approximately 20 folds in the presence of morin in MDCK/hOAT3 cells. In conclusion, the present study suggests that morin might be beneficial to reduce the nephrotoxicity of imipenem, at least in part, via the inhibition of OAT3-mediated renal excretion of imipenem.
Adsorption of Cholesterol from Milk Fat in Supercritical Carbon Dioxide
Lim,Sang-Bin,Rizvi,Syed S.H. 제주대학교 1993 논문집 Vol.36 No.-
초임계 이산화탄소에 의한 추출장치에 흡착장치를 부착하여 유지방으로부터 콜레스테롤을 제거하는 연구가 수행되었다. 실험한 여섯종의 흡착제중 실리실산과 마그네시움 실리케이트가 가장 좋은 콜레스테롤 흡착성을 보였다. 마그네시움 실리케이트는 실리실산보다 흡착제 g당 더 많은 콜레스테롤을, 그리고 더 적은 유지방을 흡착하였다. 30/60 메쉬와 60/100 메쉬의 마그네시움 실리케이트는 100/200 메쉬의 마그네시움 실리케이트보다 높은 콜레스테롤 흡착성을 보였다. 마그네시움 실리케이트의 유효용량은 90% 콜레스테롤 제거에 대해서 흡착제 g당 1.07g의 지방이었다. Reduction of cholesterol from milk fat by supercritical CO₂extraction process in conjunction with an in-line adsorption system was systematically studied. Silicic acid and magnesium silicate were found to be the most stuitable adsorbents of six adsorbents tested. Magnesium silicate adsorbed more cholesterol and less milk fat/g of adsorbent than silicic acid. Magnesium silicates of 30/60 mesh and 60/100 mesh show higher adsorption capacities than that of 100/200 mesh. The effective capacity of magnesium silicate was 1.07g of fat/g of adsorbent for 90% cholesterol reduction.