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工業系 敎師의 再敎育을 위한 硏修 프로그램의 開發에 관한 硏究
李來然,劉承坤,羅聲雄,禹鎬吉 충남대학교 공업교육연구소 1983 論文集 Vol.6 No.2(A)
This study is intended to provide a more effective curriculum for the In- Service Training Program of industrial technical teachers. The curricula which have been used during last five years were carefully analyzed and the main conclusions are suggested as follows; 1) It must be given to the teachers to take optional subjects. 2) Experiments should be reinforced make up for the understanding of theory. 3) Practical studies and factory investigations have to be provided to the teachers during the In-Service Training Program. In case of needs, the expert engineer of industries become a instructor to the teachers for explaining the up-to-date industrial techniques and theories. 4) The teachers who attending the In- Service Training Program should take an active interest in the program for getting a remarkable results.
김철원,임상용,김승곤,오만원,김성찬,이창준,남사웅,이세형,류재청 濟州大學敎 體育科學硏究所 1996 체육과학연구 Vol.2 No.-
The study was undertaken to analysis, evaluate, and prescribe the annual effect of training of periodically anaerobic and erobic motor ability for Che-Ju representive team of elementary, middle and high school athletes, first the performance can be obtained when it was prescribed proper training after analysis of physical fitness by each events, second individually fitness scientific analysis. prescription. proper management of athlete will be necessary to apply.
Helicobacter pylori와 대장균의 Shuttle Vector 개발
조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-
In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.
제주 우수 육상경기 선수들의 년중 훈련효과 분석 및 처방
김철원,임상용,김승곤,오만원,김성찬,이창준,남사웅,이세형,류재청 濟州大學敎 體育科學硏究所 1996 체육과학연구 Vol.2 No.-
The study was undertaken to analysis, evaluate, and prescribe t h e annual effect of training of periodically anaerobic and aerobic motor ability for Che-Ju representive team of elementary, middle and high school athletes. first. the performance can be obtained when it was prescribed proper training after analysis of physical fitness by each events. second, individually fitness. scientific analysis. prescription. proper management of athlete will be necessary to apply. In addition to physiology aspect, it is essential to consider sport biomechanics. sport psychology when we prescribe specific events and necessary approach in demension coach-athlete-researcher for individual.
Biological Effects of Different Thin Layer Hydroxyapatite Coatings on Anodized Titanium
Sohn, Sung-Hwa,Jun, Hye-Kyoung,Kim, Chang-Su,Kim, Ki-Nam,Ryu, Yeon-Mi,Lee, Seung-Ho,Kim, Yu-Ri,Seo, Sang-Hui,Kim, Hye-Won,Shin, Sang-Wan,Ryu, Jae-Jun,Kim, Meyoung-Kon The Korean Society of Toxicogenomics and Toxicopro 2005 Molecular & cellular toxicology Vol.1 No.4
Several features of the implant surface, such as roughness, topography, and composition play a relevant role in implant integration with bone. This study was conducted in order to determine the effects of various thin layer hydroxyapatite (HA) coatings on anodized Ti surfaces on the biological responses of a human osteoblast-like cell line (MG63). MG63 cells were cultured on A (100 nm HA coating on anodized surface), B (500-700 nm HA coating on anodized surface), C ($1{\mu}m$ HA coating on anodized surface), and control (non HA coating on anodized surface) Ti. The morphology of these cells was assessed by SEM. The cDNAs prepared from the total RNAs of the MG63 were hybridized into a human cDNA microarray (1,152 elements). The appearances of the surfaces observed by SEM were different on each of the four dental substrate types. MG63 cells cultured on A, C and control exhibited cell-matrix interactions. It was B surface showing cell-cell interaction. In the expression of several genes were up-, and down-regulated on the different surfaces. The attachment and expression of key osteogenic regulatory genes were enhanced by the surface morphology of the dental materials used.
수용액으로부터 활성탄소섬유에 의한 Cu(2), Co(2), Ni(2)흡착 및 피콜린산의 영향
유승곤,오원진,정종헌,이상문,문제권 한국화학공학회 1999 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.37 No.1
활성탄소섬유에 의한 제염 폐액 중 Cu(II), Co(II), Ni(II)의 흡착거동과 퍼콜린산 착화제의 첨가 영향을 조사하였다. 금속 이온의 흡착량은 활성탄소섬유의 표면 산도와는 일정한 관계를 보이지 않았지만 비표면적과는 비례관계를 보였다. 각 금속 이온농도에 대한 퍼콜린산의 몰농도비(Pic/M)가 1.0일 때 지배적인 화학종은 중성 전하를 띤 M(Pic)₂^0 또는 음 전하를 띤 M(Pic)₃^- 이므로 정전기적 반발력 감소로 인한 높은 흡착 용량을 얻을 수 있었다. A-20을 사용했을 때, 보다 많은 흡착 활성점 제공으로 높은 흡착 용량을 얻을 수 있었다. The adsorption behavior for Cu(II), Co(II) and Ni(II) in decontamination liquid waste on activated carbon fibers(ACFs) and the effect of picolinic acid on this adsorption have been studied. The adsorption capacity didn't have certain relation with surface acidity, but that had proportional relations with surface area. At Pic/M=1.0 dominative species are M(Pic)₂^0 with neutral charge or M(Pic)₃^- with negative charge, thus large adsorption capacity can be obtained from reducing repulsive force. At A-20 sample large adsorption capacity can be obtained from serving more active sites than the others.
유승곤,엄상용 한국화학공학회 2001 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.39 No.1
Ag, Cu와 Co를 각각 1 wt% 함유시킨 석유 핏치계 활성탄소섬유를 제조하고, 이들의 수중 페놀에 대한 흡착능력을 평가하였다. Ag, Cu함유 활성탄소섬유는 20Å 미만의 미세공이 발달하는데 비하여 Co함유 활성탄소섬유는 미세공과 함께 30-40Å, 200-300Å의 두 중기공 영역이 발달하였다. 요오드 흡착력은 Ag함유 활성탄소섬유가 우수하였으며, 메틸렌블루와 같은 고분자의 흡착은 Co함유 활성탄소섬유가 효과적이었다. 금속함유 활성탄소섬유의 페놀흡착속도는 활성탄에 비하여 18-180배 빠르며, 제거율도 우수하였다. 파과점에서 페놀의 흡착량은 금속을 함유하지 않은 활성탄소섬유가 86.4㎎/g인데 비하여 Cu함유 활성탄소섬유는 152.4 ㎎/g으로 약 1.8배의 흡착능력을 보였다. 1 wt% Ag, Cu and Co-containing Activated Carbon Fibers(ACFs) were prepared and the adsorption abilities of each ACFs for phenol from aqueous solution were investigated. 1'he average pore diameter of Ag and Co-containing were 20Å, while Co-containing ACF have shown two mesopore regions of 30-40Å and 200-300Å with micropores. Iodine adsorption capacity of Ag-containing ACF is much better then that of other ACFs. The Co-containing ACF with mesopores is more efficient than other adsorbents for the adsorption of polymer such as methyleneblue. The adsorption rate of metal-containing ACFs for phenol is 18-180 times faster than that of Activated Carbon(AC). The adsorption amount of phenol on Cu-ACF at breakpoint was 1.8 times larger than that of non metal-containing ACF.
Phosphoproteomic Analysis of AML14.3D10 Cell Line as a Model System of Eosinophilia
Ryu, Su-In,Kim, Won-Kon,Cho, Hyun-Ju,Lee, Phil-Young,Jung, Hye-Yun,Yoon, Tae-Sung,Moon, Jeong-Hee,Kang, Sung-Hyun,Poo, Ha-Ryoung,Bae, Kwang-Hee,Lee, Sang-Chul Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.5
Eosinophils act as effectors in the inflammatory reactions of allergic diseases including atopic dermatitis. Atopic dermatitis patients and others with allergic disorders suffer from eosinophilia, an accumulation of eosinophils due to increased survival or decreased apoptosis of eosinophils. In this study, a differential phosphoproteome analysis of AML14.3D10 eosinophil cell line after treatment with IL-5 or dexamethasone was conducted in an effort to identify the phosphoproteins involved in the proliferation or apoptosis of eosinophils. Proteins were separated by 2-DE and alterations in phosphoproteins were then detected by Pro-Q Diamond staining. The significant quantitative changes were shown in nineteen phosphoproteins including retinoblastoma binding protein 7, MTHSP75, and lymphocyte cytosolic protein 1. In addition, seven phosphoproteins including galactokinase I, and proapolipoprotein, were appeared after treatment with IL-5 or dexamethasone. Especially, the phospho-APOE protein was down-regulated in IL-5 treated AML14.3D10, while the more heavily phosphorylated APOE form was induced after dexamethasone treatment. These phosphoproteome data for the AML14.3D10 cell line may provide clues to understand the mechanism of eosinophilia as well as allergic disorders including atopic dermatitis.