Phosphate Transporter MdPHT1;7 Enhances Phosphorus Accumulation and Improves Low Phosphorus and Drought Tolerance

Sun Tingting,Zhou Beibei,Pei Tingting,Meng Hu,Zhang Junke,Ma Fengwang,Wei Qinping 한국식물학회 2021 Journal of Plant Biology Vol.64 No.5

Phosphorus is an important macronutrient for plant growth and is acquired by plants mainly as phosphate. Phosphate fertilizer is usually used to reduce inorganic phosphate (Pi) deficiency in the soil, improve the low phosphorus and drought tolerance of plants, and promote plant growth. Phosphate transporters (PHTs) play an important role in absorbing phosphorus from the soil. MdPHT1;7 was induced by Pi deficiency and drought in roots in our previous research. In this study, we cloned MdPHT1;7 and showed its heterologous expression can complement a high-affinity Pi transporter gene in the Pi uptake-defective yeast mutant BY4743. MdPHT1;7 is a phosphorus transporter located on the cell membrane. Overexpression of MdPHT1;7 in ‘Orin’ apple and ‘Micro-Tom’ tomato enhanced Pi accumulation, low Pi tolerance and drought tolerance. We hypothesized that MdPHT1;7 can enhance Pi absorption and play an important role in improving plant resistance to low phosphorus and drought stresses.

Anaerobic Ammonium-Oxidizing Bacteria in Cow Manure Composting

( Tingting Wang ),( Lijun Cheng ),( Wenhao Zhang ),( Xiuhong Xu ),( Qingxin Meng ),( Xuewei Sun ),( Huajing Liu ),( Hongtao Li ),( Yu Sun ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 Journal of microbiology and biotechnology Vol.27 No.7

Composting is widely used to transform waste into valuable agricultural organic fertilizer. Anaerobic ammonium-oxidizing (anammox) bacteria play an important role in the global nitrogen cycle, but their role in composting remains poorly understood. In the present study, the community structure, diversity, and abundance of anammox bacteria were analyzed using cloning and sequencing methods by targeting the 16S rRNA gene and the hydrazine oxidase gene (hzo) in samples isolated from compost produced from cow manure and rice straw. A total of 25 operational taxonomic units were classified based on 16S rRNA gene clone libraries, and 14 operational taxonomic units were classified based on hzo gene clone libraries. The phylogenetic tree analysis of the 16S rRNA gene and deduced HZO protein sequences from the corresponding encoding genes indicated that the majority of the obtained clones were related to the known anammox bacteria Candidatus “Brocadia,” Candidatus “Kuenenia,” and Candidatus “Scalindua.” The abundances of anammox bacteria were determined by quantitative PCR, and between 2.13 × 10⁵ and 1.15 × 10⁶ 16S rRNA gene copies per gram of compost were found. This study provides the first demonstration of the existence of anammox bacteria with limited diversity in cow manure composting.

The effect of crash pulse on occupant injury in frontal coach collisions

Sun Tingting,Xiong Xin,Zheng Zhuan,Shi Xiaolong,Fan Jianwei 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.11

The purpose of this study is to reveal the effect of different acceleration curves on occupant injury risk in the frontal collision of coaches. Based on characteristics of frontal crash acceleration curves, the influence of characteristic parameters on occupant injury was studied using a two-factor variance analysis method. The finite element-rigid body coupling method was used to establish a coupled simulation for the dummy and the sled and evaluate differences in the effects on occupant injury between the acceleration curves of four different coaches and the acceleration envelope of the ECE R80 standard. Results indicate that the upper limit of the simplified curve has the greatest influence on occupant injury and it displays a positive correlation. The acceleration curve stipulated by ECE R80 is too idealized, which may lead to inadequacies in safety protection capability in vehicle and seat design.

Susac Syndrome With Retinal Vasculitis: A Rare Case Report and Brief Review

Tingting Sun,Yiwen Qian,Zhiliang Wang,Yiqin Xiao 대한신경과학회 2023 Journal of Clinical Neurology Vol.19 No.5

MiR-182-5p Mediated by Exosomes Derived From Bone Marrow Mesenchymal Stem Cell Attenuates Inflammatory Responses by Targeting TLR4 in a Mouse Model of Myocardial Infraction

Sun Chuang,Li Wei,Li Yanhong,Chen Jian,An Huixian,Zeng Guangwei,Wang Tingting,Guo Yazhou,Wang Changying 대한면역학회 2022 Immune Network Vol.22 No.6

Exosomes derived from mesenchymal stem cells (MSCs) could protect against myocardial infarction (MI). TLR4 is reported to play an important role in MI, while microRNA-182-5p (miR-182-5p) negatively regulates TLR4 expression. Therefore, we hypothesize that MSCs-derived exosomes overexpressing miR-182-5p may have beneficial effects on MI. We generated bone marrow mesenchymal stem cells (BM-MSCs) and overexpressed miR-182-5p in these cells for exosome isolation. H2O2-stimulated neonatal mouse ventricle myocytes (NMVMs) and MI mouse model were employed, which were subjected to exosome treatment. The expression of inflammatory factors, heart function, and TLR4 signaling pathway activation were monitored. It was found that miR-182-5p decreased TLR4 expression in BM-MSCs and NMVMs. Administration of exosomes overexpressing miR-182-5p to H2O2-stimulated NMVMs enhanced cell viability and suppressed the expression of inflammatory cytokines. In addition, they promoted heart function, suppressed inflammatory responses, and de-activated TLR4/NF-κB signaling pathway in MI mice. In conclusion, miR-182-5p transferred by the exosomes derived from BM-MSCs protected against MI-induced impairments by targeting TLR4.

Electrochemical Degradation of Tetracycline Hydrochloride in Aqueous Medium by (B4C/C)-β-PbO2 Electrode

Tingting Xie,Hongtao Hu,Donghui Chen,Pengzhe Sun 대한화학회 2017 Bulletin of the Korean Chemical Society Vol.38 No.7

(B4C/C)-β-PbO2 electrodes were prepared by high-pressure molding technique and characterized by X-ray diffraction, scanning electron microscopy, polarization curves, cyclic voltammetry curves, and corrosion resistance test. In this paper, the effects of initial concentration, current density, electrolyte concentration, pH, and electrode spacing on tetracycline hydrochloride (TCH) degradation were investigated. Results indicated that modified electrode had high electrochemical activities, high oxygen evolution potential, and excellent corrosion resistance. The maximum removal rate of TCH could reach 90.87% at initial concentration of 300 mg/L after 120 min electrolysis with Na2SO4 concentration of 0.2 mol/L, electrode spacing of 1 cm, current density of 30 mA/cm2, and pH 3. Pseudo-first-order kinetics equation was followed during electrocatalytic degradation of TCH with 20%(B4C/C)-β-PbO2 electrode.

Fault Diagnosis for Conventional Circuit Breaker Based on One-Dimensional Convolution Neural Network

Sun Shuguang,Zhang Tingting,Wang Jingqin,Yang Feilong 대한전기학회 2023 Journal of Electrical Engineering & Technology Vol.18 No.3

The vibration signal generated by the operating mechanism of conventional circuit breaker contains abundant mechanical state information. Aiming at traditional fault diagnosis methods that need to realize signal feature extraction based on feature selection, a fault diagnosis model based on one-dimensional convolutional neural network is proposed. In the diagnosis model, multiple convolutional neural networks are designed according to the type and degree of faults, and the network is set as a large convolutional kernel to enlarge the receptive field region; the raw vibration signal is used as the model input for training, and the corresponding fault type and degree are output after hierarchical diagnosis. The experimental results show that the model can automatically extract the fault signal features, effectively complete the fault diagnosis of the contact system for the conventional circuit breaker, and has good generalization ability. The model in this paper has a higher comprehensive diagnosis recognition rate compared with other methods, reaching 98.84%.

Electrospinning Inorganic/Organic Nanohybridization Membranes with Hydrophobic and Oleophobic Performance

Tingting Zhang,Zihui Li,Ya Liu,Kangjia Ding,Yangfeng Guo,Yiyang Xu,Mengfan Sun,Dongfang Wang,Qian Li 한국섬유공학회 2023 Fibers and polymers Vol.24 No.12

Polytetrafluoroethylene (PTFE) nano-porous membrane has been widely used in various fields due to its excellent thermal stability and chemical stability. However, PTFE nanofiber membranes with simultaneous hydrophobic and oleophobic properties are essential to promote the application of PTFE. Here, based on inorganic/organic nanohybridization, we report a strategy for constructing nanostructures on fibers by adding nanoparticles, such as MOF and ZrO2. The results of FTIR and XRD confirmed MOF was synthesized successfully. At the same time, the SEM results showed UiO-66-(COOH)2 is spherical with an average diameter of 152 nm, and there is no agglomeration, which is suitable for electrospinning. Further, MOF and ZrO2 were payload into PTFE nanofibers. The results of SEM and AFM confirmed nanostructures will be more uniform and pronounced with the increase of UiO-66-(COOH)2 content, and nanostructures are most obvious when the content of UiO-66-(COOH)2 is 15%. The introduced nanostructures can increase the oil contact angle of the PTFE nano-porous membrane to 110° without introducing other groups, and further improve the water contact angle from 133° to 145°. Meanwhile, the introduction of a certain amount of hydrophilia groups can increase the oil contact angle to more than 120°. The simple strategy is of great significance to expand the application of PTFE fiber membrane in dealing with waste water treatment fields.

Lactic acid bacterial inoculant effects on the vitamin content of alfalfa and Chinese leymus silage

Tingting Jia,Zhiqiang Sun,Run Gao,Zhu Yu 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.12

Objective: Information regarding the vitamin content of silage is limited. This study investigated the changes in the vitamin content of alfalfa and Chinese leymus silages with or without a lactic acid bacterial inoculant. Methods: Alfalfa at the early flowering stage and Chinese leymus at the full-bloom stage were harvested. The treatments for each forage type were control (deionized water only) and 1×106 colony-forming units Lactobacillus plantarum (LP)/g fresh matter. After 45 days of ensiling, all silages were sampled for evaluating the vitamin content, fermentation quality and chemical composition. Results: The LP inoculant decreased the pH value and ammonia nitrogen content of the alfalfa and Chinese leymus silages and significantly (p<0.05) increased the lactic acid, acetic acid concentrations and Flieg’s points. Prior to ensiling, the levels of five B-group vitamins (thiamin, riboflavin, niacin, pantothenic acid, and pyridoxine) and α-tocopherol in alfalfa were significantly (p<0.01) higher than those in Chinese leymus. Ensiling decreased the levels of the five B-group vitamins in both alfalfa and Chinese leymus while increasing the α-tocopherol content of Chinese leymus. The thiamin, riboflavin, niacin and pantothenic acid levels in the LP-treated silage were significantly (p<0.05) lower than those in the untreated silage for the alfalfa and Chinese leymus. The α-tocopherol content in the LP-treated alfalfa silage was significantly (p<0.05) higher than that in the untreated alfalfa silage. There was no significant (p>0.05) difference in pyridoxine content between the untreated and LP-treated silages for both forages. Conclusion: With or without LP inoculation, the levels of the five B-group vitamins (thiamin, riboflavin, niacin, pantothenic acid, and pyridoxine) in alfalfa and Chinese leymus decreased after 45 days of ensiling, while the α-tocopherol content of Chinese leymus increased. The LP inoculant improved the fermentation quality of both the alfalfa and Chinese leymus silages but increased the thiamin, riboflavin, niacin, and pantothenic acid loss in the two forages after fermentation.

Primary Invasive Mucinous Adenocarcinoma of the Lung: Prognostic Value of CT Imaging Features Combined with Clinical Factors

Wang Tingting,Yang Yang,Liu Xinyue,Deng Jiajun,Wu Junqi,Hou Likun,Wu Chunyan,She Yunlang,Sun Xiwen,Xie Dong,Chen Chang 대한영상의학회 2021 Korean Journal of Radiology Vol.22 No.4

Objective: To investigate the association between CT imaging features and survival outcomes in patients with primary invasive mucinous adenocarcinoma (IMA). Materials and Methods: Preoperative CT image findings were consecutively evaluated in 317 patients with resected IMA from January 2011 to December 2015. The association between CT features and long-term survival were assessed by univariate analysis. The independent prognostic factors were identified by the multivariate Cox regression analyses. The survival comparison of IMA patients was investigated using the Kaplan-Meier method and propensity scores. Furthermore, the prognostic impact of CT features was assessed based on different imaging subtypes, and the results were adjusted using the Bonferroni method. Results: The median follow-up time was 52.8 months; the 5-year disease-free survival (DFS) and overall survival rates of resected IMAs were 68.5% and 77.6%, respectively. The univariate analyses of all IMA patients demonstrated that 15 CT imaging features, in addition to the clinicopathologic characteristics, significantly correlated with the recurrence or death of IMA patients. The multivariable analysis revealed that five of them, including imaging subtype (p = 0.002), spiculation (p < 0.001), tumor density (p = 0.008), air bronchogram (p < 0.001), emphysema (p < 0.001), and location (p = 0.029) were independent prognostic factors. The subgroup analysis demonstrated that pneumonic-type IMA had a significantly worse prognosis than solitary-type IMA. Moreover, for solitary-type IMAs, the most independent CT imaging biomarkers were air bronchogram and emphysema with an adjusted p value less than 0.05; for pneumonic-type IMA, the tumors with mixed consolidation and ground-glass opacity were associated with a longer DFS (adjusted p = 0.012). Conclusion: CT imaging features characteristic of IMA may provide prognostic information and individual risk assessment in addition to the recognized clinical predictors.