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Sun Tingting,Zhou Beibei,Pei Tingting,Meng Hu,Zhang Junke,Ma Fengwang,Wei Qinping 한국식물학회 2021 Journal of Plant Biology Vol.64 No.5
Phosphorus is an important macronutrient for plant growth and is acquired by plants mainly as phosphate. Phosphate fertilizer is usually used to reduce inorganic phosphate (Pi) deficiency in the soil, improve the low phosphorus and drought tolerance of plants, and promote plant growth. Phosphate transporters (PHTs) play an important role in absorbing phosphorus from the soil. MdPHT1;7 was induced by Pi deficiency and drought in roots in our previous research. In this study, we cloned MdPHT1;7 and showed its heterologous expression can complement a high-affinity Pi transporter gene in the Pi uptake-defective yeast mutant BY4743. MdPHT1;7 is a phosphorus transporter located on the cell membrane. Overexpression of MdPHT1;7 in ‘Orin’ apple and ‘Micro-Tom’ tomato enhanced Pi accumulation, low Pi tolerance and drought tolerance. We hypothesized that MdPHT1;7 can enhance Pi absorption and play an important role in improving plant resistance to low phosphorus and drought stresses.
Yang, Yankang,Qiu, Beibei,Chen, Shanshan,Zhou, Qiuju,Peng, Ying,Zhang, Zhi-Guo,Yao, Jia,Luo, Zhenghui,Chen, Xiaofeng,Xue, Lingwei,Feng, Liuliu,Yang, Changduk,Li, Yongfang The Royal Society of Chemistry 2018 Journal of materials chemistry. A, Materials for e Vol.6 No.20
<P>Solution-processed organic solar cells (OSCs) have been attracting more and more attention for a series of well-known advantages, and power conversion efficiencies (PCEs) of over 11% have been reported. However, the highest PCE of the OSCs based on small molecule donor/polymer acceptor blends is only 4.82%, which was much lower than those of other types of OSCs due to weak absorption of the polymer acceptor and the unbalanced charge carrier mobility of the small molecule donor and the polymer acceptor. Here, we fabricated small molecule donor/polymer acceptor-based OSCs using the wide bandgap SM1 and DR3TBDTT as the small molecular donor and the low-bandgap n-type conjugated polymer PZ1 as the polymer acceptor. With the treatment of a solvent additive, which can promote the absorption intensity, enhance the carrier mobility and suppress the charge carrier recombination, the SM1-based devices and the DR3TBDTT-based devices show PCEs of 3.97% and 5.86%, respectively. It is worth mentioning that the PCE of 5.86% is the state-of-the-art efficiency for OSCs based on the small molecular donor/polymer acceptor system.</P>
Li, Hui,Wang, Beibei,Yang, Acong,Lu, Rui,Wang, Weicheng,Zhou, Yang,Shi, Guilai,Kwon, Sung Won,Zhao, Yingming,Jin, Ying Wiley (John WileySons) 2009 Stem Cells Vol.27 No.6
<P>Embryonic stem cells (ESCs) possess the capacity to self-renew and differentiate into all cell types of an organism. It is essential to understand how these properties are controlled for the potential usage of their derivatives in clinical settings and reprogramming of differentiated somatic cells. Although transcriptional factors, such as Oct4, Sox2, and Nanog, have been considered as a part of the core regulatory circuitry, a growing body of evidence suggests that additional factors exist and contribute to the control of ESC self-renewal and differentiation. Here, we report that Ly-1 antibody reactive clone (LYAR), a zinc finger nucleolar protein highly expressed in undifferentiated ESCs, plays a critical role in maintaining ESC identity. Its downregulation significantly reduces the rate of ESC growth and increases their apoptosis. Moreover, reduced expression of LYAR in ESCs impairs their differentiation capacity, failing to rapidly silence pluripotency markers and to activate differentiation genes upon differentiation. Mechanistically, LYAR forms a complex with another nucleolar protein, nucleolin, and prevents its self-cleavage, maintaining a normal steady-state level of nucleolin protein in undifferentiated ESCs. Interestingly, the downregulation of nucleolin is detrimental to the growth of ESCs and increases the rate of apoptosis, similarly to the knockdown of LYAR. Thus, our data emphasize the fact that other genes besides Oct4 and Nanog are uniquely required for ESC self-renewal and differentiation and demonstrate that LYAR functions to control the stability of nucleolin protein, which in turn is essential for maintaining the self-renewal of ESCs.</P>
Knock-down of OsLOX by RNA interference leads to improved seed viability in rice
Suyang Bai,Niqing He,Lu Zhou,Beibei Shen,Wei Wu,Xi Liu,Ling Jiang,Jianmin Wan 한국식물학회 2015 Journal of Plant Biology Vol.58 No.5
Previous work found that lipoxygenases were key enzymes in lipid peroxidation, which causes grain deterioration during storage. In order to obtain better seed viability in rice, 10 marker-free knock-down lines were obtained in the progeny of endogenous OsLOX knock-down mutations caused by the RNAi technology. After artificial accelerated aging, there were four types of knock-down lines with higher seed viability than wild type (receptor parent). OsLOX3 knock-down line NPF1 was of special interest. In a series of experiments, including Southern blots, analysis of OsLOX3 expression, and enzymatic activity, NPF1 had better seed viability than wild-type. We also investigated the main agronomic characters of both knock-down lines and non-transgenetic wild type families. Knock-down lines were identified with generally excellent agronomic characteristics similar to the wild-type.
Development of Toxoplasma gondii Chinese I genotype Wh6 Strain in Cat Intestinal Epithelial Cells
Gui-Hua Zhao,Lixin Zhang,Lisha Dai,Haozhi Xu,Chao Xu,Ting Xiao,Jin Li,Hui Sun,Beibei Zhou,Kun Yin 대한기생충학ㆍ열대의학회 2022 The Korean Journal of Parasitology Vol.60 No.4
Felids are the unique definitive host of Toxoplasma gondii. The intestine of felid is the only site for initiating Toxoplasma gondii sexual reproduction. T. gondii excretes millions of infectious oocysts from the intestine, which are the primary source of infection. There are many difficulties in developing vaccines and drugs to control oocyst excretion due to the lack of an appropriate experimental model. Here, we established an in vitro feline intestinal epithelial cell (IEC) infection system and an efficient animal model of T. gondii Chinese 1 genotype, Wh6 strain (TgCtwh6). The Kunming mice brain tissues containing TgCtwh6 cysts were harvested 42-day post-infection. The bradyzoites were co-cultured with cat IECs in vitro at a ratio of 1:10. Five 3-month-old domestic cats were orally inoculated with 600 cysts each. The oocysts were detected by daily observation of cat feces by microscopy and polymerase chain reaction. We found that the parasite adhered and invaded cat IECs in vitro, transformed into tachyzoites, and then divided to form rose-like structures. These parasites eventually destroyed host cells, escaped, and finished the asexual reproduction process. Schizonts associated with sexual reproduction have not been observed during development in vitro cultured cells. However, schizonts were detected in all infected cat intestinal epithelial cells, and oocysts were presented in all cat feces. Our study provides a feasible cell model and an efficient infection system for the following studies of T. gondii sexual reproduction, and also lays a foundation to develop drugs and vaccines for blocking excretion and transmission of oocysts.