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- 저자 : ( Huajing Liu ) (검색결과 2 건)
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Matrine Suppresses Pancreatic Fibrosis by Regulating TGF-β/Smad Signaling in Rats
Pi Liu,Luhong Zhu,Guohui Zou,Huajing Ke 연세대학교의과대학 2019 Yonsei medical journal Vol.60 No.1
Purpose: This study aimed to elucidate the molecular mechanisms of the anti-pancreatic fibrosis effects of matrine in rats. Materials and Methods: Trinitrobenzene sulfonic acid was administrated to rats to establish a pancreatic fibrosis model. Rats weredivided into four groups: Control, Sham, Model, and Matrine (n=8). Hematoxylin-eosin staining, Masson staining, and Azan stainingwere performed to evaluate pancreatic fibrosis. Expression of transforming growth factor-β1 (TGF-β1), α-smooth muscle actin(α-SMA), and collagen I in pancreatic tissues was evaluated by immunohistochemical staining. mRNA and protein levels of TGF-βreceptor 1 (TβR1), TβR2, and Smad2 in pancreatic tissues were determined by RT-PCR and Western blot, respectively. Results: In the model group, hyperplasia of glandules around the glandular ducts, mitochondrial swelling of acinous cells, andsevere fibrosis were found. Interestingly, in the Matrine group, mitochondrial swelling was only found in a small number of acinouscells, and the fundamental structures of pancreatic tissues were intact. Moreover, pancreatic fibrosis was markedly alleviated. Comparing to the Sham group, expression of α-SMA, TGF-β1, and collagen I was sharply elevated in the Model group (p<0.05);however, their expressions were much lower in the Matrine group, compared to the Model group (p<0.05). Compared with theSham group, mRNA and protein levels of Smad2, TβR1, and TβR2 in the Model group were notably raised (p<0.05). However,their high expression was significantly downregulated in the Matrine group (p<0.05). Conclusion: Matrine suppressed pancreatic fibrosis by regulating TGF-β/Smad signaling in rats.
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Anaerobic Ammonium-Oxidizing Bacteria in Cow Manure Composting
( Tingting Wang ),( Lijun Cheng ),( Wenhao Zhang ),( Xiuhong Xu ),( Qingxin Meng ),( Xuewei Sun ),( Huajing Liu ),( Hongtao Li ),( Yu Sun ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 Journal of microbiology and biotechnology Vol.27 No.7
Composting is widely used to transform waste into valuable agricultural organic fertilizer. Anaerobic ammonium-oxidizing (anammox) bacteria play an important role in the global nitrogen cycle, but their role in composting remains poorly understood. In the present study, the community structure, diversity, and abundance of anammox bacteria were analyzed using cloning and sequencing methods by targeting the 16S rRNA gene and the hydrazine oxidase gene (hzo) in samples isolated from compost produced from cow manure and rice straw. A total of 25 operational taxonomic units were classified based on 16S rRNA gene clone libraries, and 14 operational taxonomic units were classified based on hzo gene clone libraries. The phylogenetic tree analysis of the 16S rRNA gene and deduced HZO protein sequences from the corresponding encoding genes indicated that the majority of the obtained clones were related to the known anammox bacteria Candidatus “Brocadia,” Candidatus “Kuenenia,” and Candidatus “Scalindua.” The abundances of anammox bacteria were determined by quantitative PCR, and between 2.13 × 10<sup>5</sup> and 1.15 × 10<sup>6</sup> 16S rRNA gene copies per gram of compost were found. This study provides the first demonstration of the existence of anammox bacteria with limited diversity in cow manure composting.
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