한국인 본태성 고혈압환자에서 Angiotensinogen M235T 유전자 다양성

양동호(Dong Ho Yang),홍세용(Sea Yong Hong),김홍수(Hong Soo Kim),방차옥(Cha Ok Bang),김철현(Chul Hyun Kim),최종순(Jong Soon Choi),하권수(Kwun Soo Ha) 대한신장학회 2000 Kidney Research and Clinical Practice Vol.19 No.3

N/A Background: Potential involvement of the angio-tensinogen gene(M235T) in the pathogenesis of essen-tial hypertension has been suggested by some inves- tigators. However, an association between M235T gene polymorphism and essential hypertension has been reported by some, but not by others. Since genetic diversity exists among different ethnic population, we addressed the question of whether there is an association between M235T gene poly-morphism and essential hypertension in the Korean. Methods: 100 patients with essential hypertension and 100 control subjects were recruited from out- patients at the Department of Internal Medicine, Soon-chunhyang Hospital, Chunan City. The criteria for hypertension was defined as systolic blood pressure higher than 160mmHg and/or diastolic blood pressure higher than 95mmHg. For detection of the M235T polymorphism at the angiotensinogen locus, the primer sequences were: sense primer : 5'-TGAAGGAG- AAGGTGTCTGCGGGA-3' and antisense primer : 5'- AGGACGGTGCGGTGAGAGTG-3'. The PCR product mixture was exposed to restriction enzyme Tthlll I and then submitted to electrophoresis in polyacryl-amide gel. Differences between the molecular variants of the gene in hypertensives and normotensives were com- pared by using the X²test. p<0.05 was considered statistically significant. The odds ratio and 95% confidence interval were calculated using Woolf's method. Results: Compared with the control subjects, hypertensives had higher values of three established risk factors for hypertension : age, BMI cholesterol (total and LDL). X analysis showed no difference in the distribution of genotype or allele frequency between the hypertensives and normotensives(X²=1.14, p=0.29). The crude odds ratio was 0.73 for CC over CT(95% confidence interval 0.41-1.30). The adjusted odds ratio with age, sex, lipid profilcs and BMI was 0.68 for CC over CT(9596 confidence interval 037-1,23). Conclusion : The molecular varient M235T of the angiotensinogen gene is not associated with essential hypertension in Korean population.

Antioxidant and cytoprotective effects of morin against hydrogen peroxide-induced oxidative stress are associated with the induction of Nrf-2-mediated HO-1 expression in V79-4 Chinese hamster lung fibroblasts

Lee, Moon Hee,Cha, Hee-Jae,Choi, Eun Ok,Han, Min Ho,Kim, Sung Ok,Kim, Gi-Young,Hong, Su Hyun,Park, Cheol,Moon, Sung-Kwon,Jeong, Soon-Jeong,Jeong, Moon-Jin,Kim, Wun-Jae,Choi, Yung Hyun Spandidos Publications 2017 International journal of molecular medicine Vol.39 No.3

<P>Natural phytochemicals of plant origin, including flavonoids, have been found to be potent antioxidants providing beneficial effects against oxidative stress-related diseases. The present study was carried out to investigate the antioxidant properties of morin, a flavonoid originally isolated from the flowering plants of the Moraceae family. Superoxide dismutase (SOD)-like activity and 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS(center dot+)) radical scavenging activity were determined. We also investigated the cytoprotective effects of morin against hydrogen peroxide (H2O2)-induced DNA damage and apoptosis in V79-4 Chinese hamster lung fibroblasts. Our results demonstrated that morin had strong scavenging effects against ABTS' radicals with enhanced SOD activity, which varied in a dose-dependent manner. Morin was found to reduce H2O2-induced intracellular reactive oxygen species generation and nuclear DNA damage, and it recovered cell viability damaged by H2O2 via inhibition of mitochondrial dysfunction-mediated apoptosis. Notably, the treatment of V79-4 cells with morin markedly enhanced the expression of heme oxygenase-1 (HO-1) but not quinone oxidoreductase-1, which was associated with the increased expression and phosphorylation of nuclear factor-erythroid 2-related factor 2 (Nrf2) and the downregulation of Kelch-like ECH-associated protein 1 expression. Based on our findings, we conclude that morin effectively ameliorated oxidative stress-induced DNA damage through intrinsic free radical scavenging activity and activation of the Nrf2/HO-1 pathway.</P>

Caco-2 세포 단층막 투과 실험시 교반이 약물의 투과계수에 미치는 영향

홍순선,유호정,이홍,정석재,김대덕,심창구 한국약제학회 2005 Journal of Pharmaceutical Investigation Vol.35 No.2

The unstirred water layer (UWL), which has been known to exist in the boundary of the intestinal lumen and intestinal wall, often behaves as an absorption barrier especially for lipophilic drugs. The intestinal absorption of drugs is often characterized using Caco-2 cell monolayers grown on Transwell polycarbonate membranes. The permeability (Pare) of drugs across the cell monolayer might be influenced by the agitation of the donor compartment, since the width of UWL on the surface of the cell monolayer would be reduced by the agitation. In this study, the effect of agitation of the donor compartment with 60 rpm on the permeability was measured for 12 drugs with a wide range of lipophilicity and permeability. The of mannitol, tributylmethyl ammonium, cimetidine, ranitidine, hydrocortisone, benzylpenicillin and loxoprofen was not influenced by the agitation, while the P_(app) of theophylline, propranolol, YH439, phenylpropanolarnine and testosterone was increased by the agitation. There was a significant correlation between the increase of P_(app) by agitation and the lipophilicity for the compounds having P_(app) > 2 x 10^(-5) cm/sec. No correlation was observed for the difference in P_(app) by agitation and the molecular weight, or lipophilicity of the drugs. Therefore, the agitation rate of the donor compartment in the Caco-2 cell monolayer study should be carefully controlled in order to estimate Pap, reproducibly especially for iipophilic drugs.

침전법에 의한 알루미나 분말제조 IV.ZrO₂의 분산에 의한 Al₂O₃/ZrO₂ 복합체의 제조 및 기계적 특성

홍기곤,이홍림,이형직,이호순 연세대학교 산업기술연구소 1992 논문집 Vol.24 No.2

In this study, a precipitation method, one of the most effective liquid phase reaction, was adopted in order to prepare high-tech Al₂O₃/ZrO₂ composite ceramics. Al₂(SO₄)₃·18H₂O, ZrOCl₂·8H₂O and YCl₃·6H₂O were used as starting materials and NH₄OH as a precipitation agent. Fine powders were prepared at optimum calcination condition. Sinterability of each fine powder and the effects of ZrO₂ on the grain size and mechanical properties of Al₂O₃ were investigated. The composition of Al₂O₃/ZrO₂ composites was fixed as Al₂O₃-15v/o ZrO₂(+3m/o Y₂O₃). The effect of MgO on the grain size of Al₂O₃ZrO₂ ceramics was also investigated.

당뇨병 환자의 혈청 Lipoprotein에 관한 연구

홍영숙,성낙응,경난호,이순남 梨花女子大學校 醫科大學 醫科學硏究所 1984 EMJ (Ewha medical journal) Vol.7 No.3

Lipoprotein cholesterol and triglyceride levels have been determined innormal and diabetes. The abnormalities of lipoprotein were investigated by SDS-polyacrylamide gel electrophoresis in the serum and the 3 major lipoprotein classes for diabetes. HDL cholesterol levels were lower in the diabetes compared to the normals. VLDL triglyceride levels also elevated in the diabetes. There were significant shifts in the distribution of lipoprotein cholesterol, with an increase in LDL cholesterol and decrease in HDL cholesterol. The results of this study suggest that diabetes may be associated with changes in both lipoprotein triglyceride and cholesterol levels. The electrohoretic patterns of VLDL and HDL in diabetes showed the abnorm-al pattern in comparison to normal.

綜合科學으로서의 秘書學 硏究方法論 : 理論體係 確立을 위한 問題點을 中心으로 a preliminary sketch

洪淳鎬 이화여자대학교 법정대학 비서학과 1981 비서학연구 Vol.- No.1

The purpose of this study is to exiamne the possibility of a systematic theory and methodology for developing an interisciplinary apporach to secretarial science. As a branch of the applied social sciences, secretarial science has had problems which have resulted from disagreements on the concept of "secretary". As Professor Maurice Duverger of the University of Paris has observed, there is a striking contrast between the effectivemess of the applied social sciences and the anarchic state of their theory. As in the case of all other social sciences, secretarial science suffers from a confusion on its hiatory and philosophy. This has produced two results: firstly, a mixture of the analysis of objective facts with the affirmation of normative principles; secondly, the predominance of a priori "practically". Therefore, secretarial science, as a discipline, is seen both as a science(theory) and an art(practicality). Historically , in response to practical needs, social sciences have been developing logic, methodology and standard of relevance for scientific inquiry. In order further to refine the concept of secretary, it seems useful to begin by defining the concept of administration in generic terms, as a common social process involving certain common secretarial activities. These activities, which usually include decision-making, programming, staffing wothput supervision, may be seen in most forms of business or public adminintration as well. Indeed, to some extent, the differences betweem administration and secretarial service come to rest largly in the respective environment of each. The office of the secretary may be defined as that aspect of administration occuring under the formal aegis of administrative organization at every level. While reconizing the validity of secretarial science as a subfield in the study of public and business administration, we do not believe that it involves the analysis of administration(management) in all its forms and in all human groups. We must define a "Secretarial service" differently than the way it is commonly defined. For some, secretarial service mat be characterized by its ultimate practicality. For others, it may be seen as a administrative or managerial science. However, secretarial service should be seen as neithe of these. It should be defined as something falling between public and business administration, in other wprds, as staff service within the framework of organization. The study of secretarial science should start from this point. Because secretarial science is, as mentioned above, a generalized human activity concered with ordering the men and materials required to achieve organizational goals, it has drown widly from the various social sciences. However, it is impossible to gain any adequate perspective of the field without tracing its background in the areas of public and business administration. Traditionally, the analysis of the field has reflected certain dominant contemporary trends in public and business administrative sciences which are usually recongnized as the parent discioline of the field. Each of these to be superseded as analytical tools and fashions change. An interdisciplinary apptoach represents an amalgam of all such styles: as role structural approach, behavioral approach and social philosophical approach. A new secretarial science as one of the applied social sciences should be pursued at the university level by adopting this interdisciplinary approach.

탄소성 변형을 받는 냉간 압연강의 X선 잔류응력 특성에 관한 연구

홍순혁,이동우,양광호,조석수,주원식 동아대학교 생산기술연구소 2000 生産技術硏究所硏究論文集 Vol.5 No.1

In textured material, diffraction angle 2θ usually shows a nonlinear relation against sin^2Ψ due to elastic anisotropy of crystals. SPHD and SPCD steel is cold-rolled carbon steel for automobile. The characteristics X-ray for stress measurement is Cr K_α and Mo K_α1characteristic X-ray. The sin^2Ψ-2θ diagram under elastic strain seems to have a linear behavior using regression line of data but has a nonlinear behavior in distribution of data by Cr K_αcharacteristic X-ray. As the plastic strain of specimen increases, the nonlinearity of 2θ with respect to sin^2Ψ increases remarkably. On the other hand, the diffraction angle 2 θ by Mo K_α1 characteristic X-ray shows a good linearity on sin^2Ψ-2θ diagram under plastic strain as well as elastic strain. Therefore, This paper presents the measurement of residual stress in cold-rolled carbon steel for automobile using penetration depth of Mo K_α1 charateristic X-ray and multiplicity factor of crystal diffraction plane.

대학 전산 정책의 방향설정 및 추진계획에 관한 연구

홍상은,이병수,조충호,이상정,주경수,안종근,황순기 순천향대학교 1994 논문집 Vol.17 No.1

It has been rapidly given to the importance of the embodiment of the efficient computer systems in university education. Since the recent trend of the adoption of the openess policy in university education systems significantly affects on the realization of the embodiment of the efficient computer systems which is very essential to promote the international competition in many areas of university education. So it is greatly needed that our Soonchunhyang university must have the following principles for the embodiment of the efficient computer systems. (1) It is required that the appropriate computer education and the good quality of research environment must be given. (2) The establishment of the synthetic computer network system for efficient the university administrations is needed. (3) It is needed to establish the computer system for the consideration of the future performance. (4) It is also needed to improve the organization and the administration systems of the university computer center. (5) It is required on the establishment of the local area network(LAN) systems in the Soonchunhyang university, and the LAN must be connected to other outer national and international computer systems. And we propose the following requirement for the establishment of the convienent computer systems in our university and we have been greatly though about the significant change of the environment in university computer system. [1] The subject of the basic computer must become as the required subject for the all students in the curriulum. [2] The new computer systems both in hardware and software must be introduced. [3] It is needed to the change of the present name of the university computer center into the university information and computer center. And its organizations must be furthur developed [4] The training center for the university emploee is also needed. [5] The establishment of the database systems for the automtion of the university administation is needed. [6] It is strongly required on the establishment of the local area network system in the Soonchunhang university to connect the Bitnet and the Internet through the Hankook communication. [7] The computerization for the central library is needed. The indepent local area network system for the library is also needed to establish the own database system. Finally we hope that the LAN will be connected to the national wide library computer system.

보행시 하지체질의 내적일 분석

홍성표,이순호,김희수 忠南大學校體育科學硏究所 1995 體育科學硏究誌 Vol.13 No.1

This study is to represent basic data of biomechanical work of human body which has been data measured precisely and is to see on pathology and abnormal gaits patterns. And 5, 6th grade boys in Taejon city were attended in this experiment. Each student wore 8 landmarks on their lower leg joints and walked for 4 Km/h, 5 Km/h, and 6 Km/h on the treadmill Mechanical energy of lower extremity segment by lower joint (thigh and shank) movement were measured in stance phase and swing phase which were assessed internal work and energy of segment The conclusions were as following; 1. Increasing gait velocity cause toe off in early stage and swing of lower extremity happen early and quickly with angle of shank increasing in swing phase. 2. Children gait velocity grow rapidly above 5km/h, internal work of thigh mechanical energy showed value of work clearly and large. and internal work change represent greater in shank than in thigh 3. The stance period of gait is the period off set by two operated cocontracting muscles, and the later the speed of gait is, the lower the effect of the net torque.

자전거의 페달반력 측정 시스템 개발

홍성표,이창섭,이순호 忠南大學校體育科學硏究所 1997 體育科學硏究誌 Vol.15 No.1

The purpose of this study was to develop the measurement system of pedal reaction-force for the bicycle that measures two-dimension force with loadcell on the pedal. The system consisted of sensor part, data collection part and software part for control. The sensor part consisted of preamplier and signal condition circuit. It was consisted of high intensity circuit to decrease the noise and to improve response trait. The data collection part with 12 bit was collected for the data by timing mode on trigger signal. The signal of the sensor was controlled for the sampling speed by way of the software in computer. Measuring system on pedal reaction force testified the utilization through calibration and the experimental test.