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Jin-Ung Yun,Bo-Kyong Kim,Seong-Beom Jin,Soon Young Ahn,Young Hyo Kim,Doo-Khil Moon,Kwan Jeong Song 한국원예학회 2006 Horticulture, Environment, and Biotechnology Vol.47 No.5
To develop an efficient embryogenic culture in satsuma mandarin, some factors related to physiological and environmental conditions were investigated during embryogenic culture initiation from undeveloped seeds of ‘Miyagawa Wase’ and ‘Okitsu Wase’ satsuma mandarin. Much more undeveloped seeds were obtained from larger fruits and the number of large seeds was positively correlated with fruit size. However, large seeds showed lower frequency in initial callus induction and somatic embryogenesis. Effects of culture condition, medium type, carbohydrate source, and agar concentration on initial callus induction and embryogenesis were further evaluated. There was no effect of culture condition of darkness or light. MT and l/2EME media were effective in ‘Miyagawa Wase’ and ‘Okitsu Wase’, respectively. In the experiment of carbohydrate source, 3% sucrose was more effective than 5% sucrose or 2% glycerol. In addition, agar concentration higher than 1.2% was favorable to callus induction in both cultivars.
Identification of Zygotic and Nucellar Mandarin Seedlings Using Randomly Amplified Polymorphic DNA
Jin-Ung Yun,Hee Beom Yang,Yong-Hwan Jung,Su-Hyun Yun,Kwang Sik Kim,Chan-Shick Kim,Kwan Jeong Song 한국원예학회 2007 Horticulture, Environment, and Biotechnology Vol.48 No.3
The study aimed at evaluating an efficiency of cross breeding in ‘Miyagawa Wase’ and ‘Okitsu Wase’ satsuma mandarin and ‘Shiranuhi’ mandarin through investigating the seed and seedlings formation, the frequency and position of zygotic seedlings, and the influence of pollen parents using randomly amplified polymorphic DNA (RAPD). Seedlings were in vitro cultured with seeds obtained from four crosses of ‘Miyagawa Wase’ (Citrus unshiu) × ‘Ponkan’ (C reticulata), ‘Okitsu Wase’ (C unshiu) × ‘Swingle Citrumelo’ (C. paradisi × Poncirus trifoliate), ‘Shiranuhi’ [(C unshiu × C. sinensis) × C. reticulata] × ‘Ponkan’ (C. reticulata), and ‘Shiranuhi’ [(C. unshiu × C. sinensis) × C. reticulata] × ‘Swingle Citrumelo’ (C. paradisi × P. trifoliate). RAPD analysis was conducted with five primers selected from screening 77 primers based on DNA amplification of parent plants. Seed formation including total seeds and full sized seeds per fruit between ‘Miyagawa Wase’ and ‘Okitsu Wase’ was not different statistically. However, seed formation of ‘Shiranuhi’ mandarin was significantly affected by pollen genotype. Average seedlings number per seed was not different effectively. The frequency and positioning patterns of zygotic seedling were different in each cultivar. The results suggest that seed formation, polyembryony, and frequency and positioning of zygotic seedling in some citrus having polyembryony might be affected by seed and pollen parents.
Ahn, Chang-Beohm,Noh Joon-Yong,윤현민,Kim Cheol-hong,Song Ung-Kwan,Ahn Chang-beohm 대한약침학회 2021 Journal of pharmacopuncture Vol.24 No.4
The aim of this study was to report on the improvement of shoulder pain resulting from disorders of the rotator cuff such as impingement syndrome and adhesive capsulitis, by manual acupuncture (MA) and pharmacopuncture (PA) following origin/insertion technique (OIT) of applied kinesiology (AK). Two patients were treated with MA and PA after OIT on shoulder muscles. The Numerical Rating Scale and the assessment of the Japanese Orthopedic Association scores were used to assess the pain, and ultrasound images were taken to compare treatment outcome. This study showed that MA and PA following OIT may be an effective treatment for impingement syndrome and adhesive capsulitis.