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      • KCI등재
      • «Activités interactives en classe d'expression orale»

        ( Liu Shun-i ) 한국프랑스어문교육학회 2008 한국프랑스어문교육학회 학술대회자료집 Vol.2008 No.-

        En raison du contexte d'apprentissage du français dans lequel l'accent étant mis sur l'aspect écrit de la langue, ils ont peu d'occasions de pratiquer cette langue, les apprenants taïwanais éprouvent des difficultés pour s'exprimer dans cette langue dans le cours d'expression orale. D'autant qu'à ce contexte s'ajoutent des obstacles d'ordre psychologique. L'application d'une pédagogie interactive centrée sur l'apprenant, et passant par la mise en oeuvre d'activités interactives, serait à méme d'y remédier.

      • KCI등재

        FSCB phosphorylation in mouse spermatozoa capacitation

        ( Shun Li Liu ),( Bing Ni ),( Xiang Wei Wang ),( Wen Qian Huo ),( Jun Zhang ),( Zhi Qiang Tian ),( Ze Min Huang ),( Yi Tian ),( Jun Tang ),( Yan Hua Zheng ),( Feng Shuo Jin ),( Yan Feng Li ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.8

        It is generally accepted that spermatozoa capacitation is associated with protein kinase A-mediated tyrosine phosphorylation. In our previous study, we identified the fibrous sheath CABYR binding protein (FSCB), which was phosphorylated by PKA. However, the phosphorylation status of FSCB protein during spermatozoa capacitation should be further investigated. To this aim, in this study, we found that phosphorylation of this 270-kDa protein occurred as early as 1 min after mouse spermatozoa capacitation, which increased over time and remained stable after 60 min. Immunoprecipitation assays demonstrated that the tyrosine and Ser/Thr phosphorylation of FSCB occurred during spermatozoa capacitation. The extent of phosphorylation and was closely associated with the PKA activity and spermatozoa motility characteristics. FSCB phosphorylation could be induced by PKA agonist DB-cAMP, but was blocked by PKA antagonist H-89.Therefore, FSCB contributes to spermatozoa capacitation in a tyrosine-phosphorylated format, which may help in further elucidating the molecular mechanism of spermatozoa capacitation. [BMB reports 2011; 44(8): 541-546]

      • KCI등재

        唐李贤太子墓《东客使图》中的三位朝鲜半岛使节

        ??利(Shun-Li Liu) 중국어문논역학회 2013 中國語文論譯叢刊 Vol.0 No.33

        The three envoys on Prince Li Xian tomb’s mural were korean, they were the envoy of Silla, Baekje and Goguryeo. The image of the envoy of Silla liked as the image of one of the famous Silla monk, the monk’name was Yi Xiang. Baekje’s envoy had same clothes style in recording of ancient time in Korean Peninsula. Goguryeo’s envoy stood far away from them, it seems a bit hesitant. Li Xian had been the king of Hebei, who was directly responsible for the foreign affairs of these three countries, so there were three envoys images of Korean Peninsula on his tomb’s mural.

      • KCI등재

        Experimental Investigations on VIV of Bridge Deck Sections: A Case Study

        Zeng-shun Chen,Si-meng Liu,Xianfeng Yu,Cun-ming Ma,Lei Liu 대한토목학회 2017 KSCE Journal of Civil Engineering Vol.21 No.7

        In this paper, the Vortex Induced Vibrations (VIVs) of bridge deck sections of the Hong Kong-Zhuhai-Macao Bridge were investigated experimentally. Aeroelastic tests for the bridge deck of two scale ratios (1:20 model and 1:50 model) under different wind attack angles, wind speeds, and damping ratios were performed. Accessory and windbreak effects on the VIVs of the bridge deck were also carried out. The experimental results show that the accessory and windbreak tend to enlarge the VIVs of the bridge deck. Furthermore, the most unfavorable wind attack angle is 5o. At this attack angle and low damping ratios, the VIVs of the bridge deck are significant and much larger than the allowable value. In addition, the VIVs of the bridge deck decrease with increasing the damping ratios (Particularly, at large damping ratios, the VIVs were well suppressed). This study provides a guideline for the designs of long span flexible bridges on suppressing the VIVs of the bridges.

      • KCI등재

        Cloning and Molecular Characterization of a Novel Rolling-Circle Replicating Plasmid, pK1S-1, from Bacillus thuringiensis subsp. kurstaki K1

        Ming Shun Li,노종열,Xueying Tao,Zi Niu Yu,Zi Duo Liu,Qin Liu,Hong Guang Xu,심희진,김양수,왕용,최재영,제연호 한국미생물학회 2009 The journal of microbiology Vol.47 No.4

        Bacillus thuringiensis, an entomopathogenic bacterium belonging to the B. cereus group, harbors numerous extra-chromosomal DNA molecules whose sizes range from 2 to 250 kb. In this study, we used a plasmid capture system (PCS) to clone three small plasmids from B. thuringiensis subsp. kurstaki K1 which were not found in B. thuringiensis subsp. kurstaki HD-1, and determined the complete nucleotide sequence of plasmid pK1S-1 (5.5 kb). Of the six putative open reading frames (ORF2-ORF7) in pK1S-1, ORF2 (MobK1) showed approximately 90% aa identity with the Mob-proteins of pGI2 and pTX14-2, which are rolling circle replicating group VII (RCR group VII) plasmids from B. thuringiensis. In addition, a putative origin of transfer (oriT) showed 95.8% identity with those of pGI2 and pTX14-2. ORF3 (RepK1) showed relatively low aa identity (17.8~25.2%) with the Rep protein coded by RCR plasmids, however. The putative double- strand origin of replication (dso) and single-strand origin of replication (sso) of pK1S-1 exhibited approximately 70% and 64% identities with those of pGI2 and pTX14-2. ORF6 and 7 showed greater than 50% similarities with alkaline serine protease, which belongs to the subtilase family. The other 2 ORFs were identified as hypothetical proteins. To determine the replicon of pK1S-1, seven subclones were contructed in the B. thuringiensis ori-negative pHT1K vector and were electroporated into a plasmid cured B. thuringiensis strain. The 1.6 kb region that included the putative ORF3 (Rep1K), dso and ORF4, exhibited replication ability. These findings identified pK1S-1 as a new RCR group VII plasmid, and determined its replication region.

      • KCI등재

        중첩세대간 경제의 유일성과 안정성 연구

        류순희 ( Shun J Liu ),박현 ( Hyun Park ) 경희대학교 사회과학연구원 2013 社會科學硏究 Vol.39 No.2

        본 연구는 가계의 시간에 대한 선호도가 내생적으로 결정되는 세대간 중첩모형에서의 동태적 일반균형의 특징을 살펴본다. 특히, 각 가계의 효용함수의 시간선호율은 부모의 소비및 저축에 대해 음(-)의 관계가 있고, 동세대의 다른 가계들의 평균소비 및 자산에 대해서는 양(+)의 관계가 있다는 가정 하에서 동태적 균형의 존재성, 유일성, 안정성을 연구한다. 첫째, 개인의 효용에 미치는 외부성으로 인하여 다중균형이 존재할 수 있다. 이는 동태적완전경쟁 경제에서 가계 간의 소득불균형의 원인을 내포하고 있다. 둘째, 본 논문은 장기적균형성장 궤적과 과도적 궤적의 유일성과 안정성 조건을 제시한다. 셋째, 시간선호율이 내생적인 모형에서 장기균형의 지엽적 안정성 및 안정적 소용돌이의 발생할 수 있음을 보여주며, 이는 내생적인 경기변동이 장/단기 시장균형에서 모두 발생할 수 있는 현상이라는 함의가 있다. This study considers a dynamic competitive general equilibrium when time preferences in life-cycle utility are endogenous. In an economy with overlapping generations, we postulate that each household`s time preferences are not only influenced by the parent`s consumption and savings behavior in a dynastic intergenerational environment, but also affected by external consumption habits in young cohort households in an intragenerational environment. This paper shows the existence, uniqueness, and stability of the dynastic competitive economy with endogenous time preferences. First, the effect of these consumption externalities on the individual`s time preferences generates the existence of multiple equilibrium paths. This suggests the possibility of income equalities among households in a dynamic competitive economy. Second, we also identify the conditions needed for a unique balanced growth path and a stable transitional path. Third, we study the dynamics of a transitional path based around a steady state and demonstrate the possibility of saddle stabilities and stable spirals, thus suggesting the existence of endogenous business cycles.

      • Cloning and Molecular Characterization of a Novel Rolling-Circle Replicating Plasmid, pK1S-1, from Bacillus thuringiensis subsp. kurstaki K1

        Ming Shun Li,Jong Yul Roh,Xueying Tao,Zi Niu Yu,Zi Duo Liu,Qin Liu,Hong Guang Xu,Hee Jin Shim,Yang-Su Kim,Yong Wang,Jae Young Choi,Yeon Ho Je 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05

        Bacillus thuringiensis, an entomopathogenic bacterium belonging to the B. cereus group, harbors numerous extra-chromosomal DNA molecules whose sizes range from 2 to 250 kb. In this study, we used a plasmid capture system (PCS) to clone three small plasmids from B. thuringiensis subsp. kurstaki K1 using PCS which were not found in B. thuringiensis subsp. kurstaki HD-1, and determined the complete nucleotide sequence of plasmid pK1S-1 (5.5 kb). Of the six putative open reading frames (ORF2-ORF7) in pK1S-1, ORF2 (MobK1) showed approximately 90% aa identity with the Mob-proteins of pGI2 and pTX14-2, which are rolling circle replicating group VII (RCR group VII) plasmids from B. thuringiensis. In addition, a putative origin of transfer (oriT) showed 95.8% identity with those of pGI2 and pTX14-2. ORF3 (RepK1) showed relatively low aa identity (17.8-25.2%) with the Rep protein coded by RCR plasmids, however. The putative double-strand origin of replication (dso) and single-strand origin of replication (sso) of pK1S-1 exhibited approximately 70% and 64% identities with those of pGI2 and pTX14-2. ORF6 and 7 showed greater than 50% similarities with alkaline serine protease, which belongs to the subtilase family. The other 2 ORFs were identified as hypothetical proteins. To determine the replicon of pK1S-1, seven subclones were contructed in the B. t huringiensis ori-negative pHT1K vector and were electroporated into a plasmid cured B. thuringiensis strain. The 1.6 kb region that included the putative ORF3 (Rep1K), dso and ORF4, exhibited replication ability. These findings identified pK1S-1 as a new RCR group VII plasmid, and determined its replication region.

      • miR-200a Inhibits Tumor Proliferation by Targeting AP-2γ in Neuroblastoma Cells

        Gao, Shun-Li,Wang, Li-Zhong,Liu, Hai-Ying,Liu, Dan-Li,Xie, Li-Ming,Zhang, Zhi-Wei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.11

        Background: MicroRNA-200a (miR-200a) has been reported to regulate tumour progression in several tumours but little is known about its role in neuroblastoma. Our aim was to investigate the potential role and mechanism of miR-200a in neuroblastomas. Materials and Methods: Expression levels of miR-200a in tissues were determined using RT-PCR. The effect of miR-200a and shAP-$2{\gamma}$ on cell viability was evaluated using MTS assays, and target protein expression was determined using Western blotting and RT-PCR. Luciferase reporter plasmids were constructed to confirm direct targeting. Results were reported as mean${\pm}$S.E.M and differences were tested for significance using the 2-tailed Students t-test. Results: We determined that miR-200a expression was significantly lower in neuroblastoma tumors than the adjacent non-cancer tissue. Over-expression of miR-200 are reduced cell viability in neuroblastoma cells and inhibited tumor growth in mouse xenografts. We identified AP-$2{\gamma}$ as a novel target for miR-200a in neuroblastoma cells. Thus miR-200a targets the 3'UTR of AP-$2{\gamma}$ and inhibits its mRNA and protein expression. Furthermore, our result showed that shRNA knockdown of AP-$2{\gamma}$ in neuroblastoma cells results in significant inhibit of cell proliferation and tumor growth in vitro, supporting an oncogenic role of AP-$2{\gamma}$ in neuroblastoma. Conclusions: Our study revealed that miR-200a is a candidate tumor suppressor in neuroblastoma, through direct targeting of AP-$2{\gamma}$. These findings re-enforce the proposal of AP-$2{\gamma}$ as a therapeutic target in neuroblastoma.

      • KCI등재

        Thermometry for Dirac Fermions in Graphene

        Fan-Hung Liu,Chang-Shun Hsu,Shun-Tsung Lo,Chiashain Chuang,Lung-I Huang,Tak-Pong Woo,Chi-Te Liang,Y. Fukuyama,Y. Yang,R. E. Elmquist,Pengjie Wang,Xi Lin 한국물리학회 2015 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.66 No.1

        We use both the zero-magnetic-field resistivity and the phase coherence time determined by weaklocalization as independent thermometers for Dirac fermions (DF) in multilayer graphene. In thehigh current (I) region, there exists a simple power law TDF / I0.5, where TDF is the effective Diracfermion temperature for epitaxial graphene on SiC. In contrast, TDF / I1 in exfoliated multilayergraphene. We discuss possible reasons for the different power laws observed in these multilayergraphene systems. Our experimental results on DF-phonon scattering may find applications ingraphene-based nanoelectronics.

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