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Shen, Chaohui,Gao, Xiyang,Li, Tao,Zhang, Jun,Gao, Yuqian,Qiu, Liyou,Zhang, Guang The Korean Society of Mycology 2019 Mycobiology Vol.47 No.3
The $11{\alpha}$-hydroxylation of $16{\alpha}$, 17-epoxyprogesterone (EP) catalyzed by Rhizopus nigricans is crucial for the steroid industry. However, lower conversion rate of the biohydroxylation restricts its potential industrial application. The $11{\alpha}$-steroid hydroxylase CYP509C12 from R. oryzae were reported to play a crucial role in the $11{\alpha}$-hydroxylation in recombinant fission yeast. In the present study, the CYP509C12 of R. oryzae (RoCYP) was introduced into R. nigricans using the liposome-mediated mycelial transformation. Heterologous expression of RoCYP resulted in increased fungal growth and improved intracellular reactive oxygen species content in R. nigricans. The $H_2O_2$ levels in RoCYP transformants were approximately 2-folder that of the R. nigricans wild type (RnWT) strain, with the superoxide dismutase activities increased approximately 45% and catalase activities decreased approximately 68%. Furthermore, the $11{\alpha}$-hydroxylation rates of EP in RoCYP transformants (C4, C6 and C9) were 39.7%, 38.3% and 38.7%, which were 12.1%, 8.2% and 9.4% higher than the rate of the RnWT strain, respectively. This paper investigated the effect of heterologous expression of RoCYP in R. nigricans, providing an effective genetic method to construct the engineered strains for steroid industry.
( Chaohui Shen ),( Xiyang Gao ),( Tao Li ),( Jun Zhang ),( Yuqian Gao ),( Liyou Qiu ),( Guang Zhang ) 한국균학회 2019 Mycobiology Vol.47 No.3
The 11a-hydroxylation of 16a, 17-epoxyprogesterone (EP) catalyzed by Rhizopus nigricans is crucial for the steroid industry. However, lower conversion rate of the biohydroxylation restricts its potential industrial application. The 11a-steroid hydroxylase CYP509C12 from R. oryzae were reported to play a crucial role in the 11a-hydroxylation in recombinant fission yeast. In the present study, the CYP509C12 of R. oryzae (RoCYP) was introduced into R. nigricans using the liposome-mediated mycelial transformation. Heterologous expression of RoCYP resulted in increased fungal growth and improved intracellular reactive oxygen species content in R. nigricans. The H<sub>2</sub>O<sub>2</sub> levels in RoCYP transformants were approximately 2-folder that of the R. nigricans wild type (RnWT) strain, with the superoxide dismutase activities increased approximately 45% and catalase activities decreased approximately 68%. Furthermore, the 11a-hydroxylation rates of EP in RoCYP transformants (C4, C6 and C9) were 39.7%, 38.3% and 38.7%, which were 12.1%, 8.2% and 9.4% higher than the rate of the RnWT strain, respectively. This paper investigated the effect of heterologous expression of RoCYP in R. nigricans, providing an effective genetic method to construct the engineered strains for steroid industry.
高申(Shen Gao),蒲川(Chuan Pu),王宝秦(Baoqin Wang),蒋祎(Yi Jiang) 아시아사회과학학회 2023 International Science Research Vol.3 No.3
元认知是对认知的认知,其与案例教学结合的优势体现在学生通过重新认知案例教学过程和学习行为,可以强化案例教学的效果。本研究是在研究生《医疗卫生法律法规》课程中开展基于元认知的案例教学以期对课程思政的教学方式进行创新。基于元认知的案例教学实践分为四个部分:制定教学目标、形成课程思政案例微课库、开展案例教学和教学评价。学生评价结果表明,基于元认知理论进行课程思政案例教学能显著提升学习效果,计划、监控和调节是提升元认知能力的三大策略。 Metacognition is the cognition of cognition, and the advantage of its combination with case teaching is reflected in that students can strengthen the effect of case teaching by re-recognizing the process and learning behavior of case teaching. This study is to carry out metacognition-based case teaching in the course of Medical Health Laws and Regulations for postgraduate students in order to innovate the teaching methods of ideological and political education. The practice of case teaching based on metacognition is divided into four parts: formulating teaching objectives, forming the micro-class library of curriculum ideological and political cases, carrying out case teaching and teaching evaluation. The evaluation results of students show that the course case teaching based on metacognitive theory can significantly improve the learning effect, and planning, monitoring and adjustment are the three strategies to improve the metacognitive ability.
Aldehyde Volatiles Emitted in Succession from Mechanically Damaged Leaves of Poplar Cuttings
( Zeng Hui Hu ),( Ying Bai Shen ),( You Qing Luo ),( Fan Yi Shen ),( Hai Bo Gao ),( Rong Fu Gao ) 한국식물학회 2008 Journal of Plant Biology Vol.51 No.4
Plant aldehydes are volatiles necessary to defenses against environmental stress. To explore their emissions in response to wounding, we performed gas chromatography-mass spectrometry (GC-MS) on cuttings from poplar (Populus simonii×P. pyramidalis ``Opera 8277``) that were mechanically damaged to mimic herbivore attack. We detected 16 aldehydes, including 11 linear saturated aldehydes, 3 linear unsaturated aldehydes, and 2 non-linear aldehydes. Emissions of these aldehydes were clearly enhanced by such treatment, and exhibited a similar pattern of change, i.e., increasing in the first 2 h, then sharply decreasing before rising again at about 12 h. Two release peaks for these aldehydes were observed. Therefore, we propose two pathways for the mediation of aldehyde emissions following damage. The first peak may represent emissions from plant storage pools, whereas the second release peak might result from greater formation de novo through an activated synthesis pathway.
Gao Ma,Xiao-Quan Xu,Hao Hu,Guo-Yi Su,Jie Shen,Hai-Bin Shi,Fei-Yun Wu 대한영상의학회 2018 Korean Journal of Radiology Vol.19 No.3
Objective: To compare the diagnostic performance of readout-segmented echo-planar imaging (RS-EPI)-based diffusion kurtosis imaging (DKI) and that of diffusion-weighted imaging (DWI) for differentiating malignant from benign masses in head and neck region. Materials and Methods: Between December 2014 and April 2016, we retrospectively enrolled 72 consecutive patients with head and neck masses who had undergone RS-EPI-based DKI scan (b value of 0, 500, 1000, and 1500 s/mm2) for pretreatment evaluation. Imaging data were post-processed by using monoexponential and diffusion kurtosis (DK) model for quantitation of apparent diffusion coefficient (ADC), apparent diffusion for Gaussian distribution (Dapp), and apparent kurtosis coefficient (Kapp). Unpaired t test and Mann-Whitney U test were used to compare differences of quantitative parameters between malignant and benign groups. Receiver operating characteristic curve analyses were performed to determine and compare the diagnostic ability of quantitative parameters in predicting malignancy. Results: Malignant group demonstrated significantly lower ADC (0.754 ± 0.167 vs. 1.222 ± 0.420, p < 0.001) and Dapp (1.029 ± 0.226 vs. 1.640 ± 0.445, p < 0.001) while higher Kapp (1.344 ± 0.309 vs. 0.715 ± 0.249, p < 0.001) than benign group. Using a combination of Dapp and Kapp as diagnostic index, significantly better differentiating performance was achieved than using ADC alone (area under curve: 0.956 vs. 0.876, p = 0.042). Conclusion: Compared to DWI, DKI could provide additional data related to tumor heterogeneity with significantly better differentiating performance. Its derived quantitative metrics could serve as a promising imaging biomarker for differentiating malignant from benign masses in head and neck region.
Academia or enterprises: gender, research outputs, and employment among PhD graduates in China
Shen Wenqin,Gao Yao,Zhang Bin,Jiang Jin 서울대학교 교육연구소 2018 Asia Pacific Education Review Vol.19 No.2
PhD graduates play an important role in the national innovation system. However, only a few studies have explored Chinese PhD graduates’ employment and development prospects because of limited data. To fill in this gap, this study conducted a survey of doctoral recipients in 13 Chinese universities. The authors drew on the national survey data and in-depth interviews to critically analyze the PhD graduates’ job preferences, employment outcomes, and factors that affect their employment sectors. Results indicate a notable difference between the graduates’ job preferences and their employment outcomes. The proportion of employment in academia is substantially below expected, particularly among female graduates. This study specifically focuses on the impact of gender and scientific output (including articles and patents) on the employment outcomes of PhD graduates. Compared with graduates employed by the non-academic sectors, male graduates have a lower probability of employment in universities and research institutions. Compared with those working in enterprises, PhD graduates employed in universities and research institutions published more during their doctoral study. For graduates who majored in science, engineering, and agricultural sciences, having patents increases their probability of working in the enterprises.
Environmental fatigue correction factor model for domestic nuclear-grade low-alloy steel
Gao, Jun,Liu, Chang,Tan, Jibo,Zhang, Ziyu,Wu, Xinqiang,Han, En-Hou,Shen, Rui,Wang, Bingxi,Ke, Wei Korean Nuclear Society 2021 Nuclear Engineering and Technology Vol.53 No.8
Low cycle fatigue behaviors of SA508-3 low-alloy steel were investigated in room-temperature air, high-temperature air and in light water reactor (LWR) water environments. The fatigue mean curve and design curve for the low-alloy steel are developed based on the fatigue data in room-temperature and high-temperature air. The environmental fatigue model for low-alloy steel is developed by the environmental fatigue correction factor (F<sub>en</sub>) methodology based on the fatigue data in LWR water environments with the consideration of effects of strain rate, temperature, and dissolved oxygen concentration on the fatigue life.
Shen Guanghai,Kiem Phan Van,Cai Xing-Fu,Li Gao,Dat Nguyen Tien,Choi Yeon A,Lee Young Mi,Park Yong Ki,Kim Young Ho The Pharmaceutical Society of Korea 2005 Archives of Pharmacal Research Vol.28 No.6
A new biflavonol glycoside named as solanoflavone (1) was isolated from aerial part of Solanum melongena. The chemical structure was elucidated as isorhamnetin-3-O-$\beta$-D-glucopyranoside-(4'$\to$O$\to$4"')-galangin-3"-O-$\to$-D-glucopyranoside on the basis of physicochemical and spectroscopic methods, including 2D NMR spectral techniques.