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Kho, Kang Hee,Kim, Jung Woo,Kim, Soo Cheol,Choi, Myeong Rak,Han, Kyeong Ho,Lee, Won Kyo,Choi, Kap Seong The Korean Society for Applied Biological Chemistr 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.5
Carbonic anhydrases (CAs) play a key role in physiological functions such as pH homeostasis, calcification, photosynthesis, and ionic regulations. Among CA isozymes, CA I is a specific marker for the cytoplasm or apical cell membranes of colonic epithelial cells, and it is related to enterocyte proliferation in mammals. The major aim of the present study was to investigate the regional, cellular, and subcellular localization of CA I in a fish, rainbow trout, Oncorhynchus mykiss. The CA I was identified in the gills, heart, and intestine of the rainbow trout. CA I protein detected in the tissues of the rainbow trout was similar to a CA III with a molecular weight of 29 kDa. Immunohistochemical results demonstrated that CA I was localized in the various cells of gills, heart, muscle, and intestine of the rainbow trout.
반연속배양의 혼합균주에 의한 Benzene, Toluene 및 Phenol 혼합물 분해
오희목,김서인,이창호,서현효,이문호,고영희,윤병대 한국산업미생물학회 1994 한국미생물·생명공학회지 Vol.22 No.4
Benzene, toluene 및 phenol을 각기 250 ㎎/ℓ 포함한 합성폐수에 혼합균주인 GE1을 접종하고 반연속배양에 의하여 군집의 안정을 이룬 후 방향족화합물의 생분해에 대하여 조사하였다. 미생물 무처리구인 대조구에서 75 ㎖/ℓ/min의 폭기에 의해 배양 24시간에 phenol은 37%, benzene은 83%가 휘발되고, toluene은 배양 12시간에 모두 휘발되었다. Benzene과 toluene의 생분해는 섬모상 고정화 담체를 설치한 SB(strains+biofilm) 처리구에서 효과적이며, phenol은 glucose를 첨가한 SG(strains+glucose) 처리구에서 보다 신속히 분해되었다. Benzene, toluene 및 phenol을 250 ㎎/ℓ 또는 1000 ㎎/ℓ 첨가하였을 때 SG 처리구는 16시간이나 32시간에 이들 화합물을 완전히 제거하였다. COD 제거율은 SG 처리구에서 초기에 80 ㎎/ℓ/h로 가장 높았으나, 증식된 균체량에 의하여 배양 12시간 이후에는 116∼140 ㎎/ℓ의 일정한 값을 보였다. 결론적으로 혼합균주 GE1은 benzene, toluene 그리고 phenol 등의 방향족화합물이 혼합된 산업폐수의 처리에 폭 넓게 사용될 수 있는 가능성을 보였다. The biodegradation of aromatic compounds by a mixed culture GE1 was investigated in an artificial wastewater containing 250 ㎎/ℓ of benzene, toluene, and phenol in semicontinuous culture. In the control group (no strains) with an aeration rate of 75 ㎖/ℓ/min, 37% of phenol and 83% of benzene were volatilized during early 24 hrs and toluene was disappeared form the medium within 12 hrs. The biodegradation of benzene and toluene was effective in SB (strains+biofilm) treatment, while phenol was degrade more quickly in SG (strains+glucose) treatment including glucose as an additional carbon source. Aromatic compounds added at a concentration of 250 ㎎/ℓ or 1000 ㎎/ℓ were completely removed by SG treatment after 16 hrs or 32 hrs, respectively. The removal rate of COD was high as much as 80 ㎎/ℓ/h in SG treatment during early period, but COD revealed a stable value of 116∼140 ㎎/ℓ after 12 hrs caused by increased biomass. Therefore, it is concluded that the mixed culture GE1 could be used for the wastewater treatment including aromatic compounds such as benzene, toluene, and phenol.