Bacillus sp. SK31의 생물흡착제 흡착특성 및 생산

서현효,김형갑 한국지하수토양환경학회 1999 지하수토양환경 Vol.4 No.2

흡착제 생산균주를 선별하기 위하여 토양시료에서 분리한 흡착제 생산균주중 가장 뛰어난 흡착물질을 생산하는 균주, SK31을 선별하였으며 분리균주 SK31은 Bacillus 속으로 동정되었다. Bacillus sp. SK31에 의하면 생산된 흡착물질(생물흡착제 SK31)은 ethanol 침전과 cetylpyridinium(CPC) 침전을 통하여 정제하여, 아연과 납 이온에 대한 흡착특성을 조사하였다. 생물흡착제 SK31의 아연과 잡 이온의 흡착량은 각각 52 mg/g과 112 mg/g 이었다. Bacillus sp. SK31 균주가 흡착제를 생산하기 위한 flask 수준에서의 배양조건들이 조사되었다. 흡착제 생산을 위한 최적 pH와 최적온도는 각각 7.5와 30℃로 나타났다. 흡착제 생산시 주요한 탄소원과 질소원은 glucose와 ammonium nitrate이었다. 최적화된 배지에서의 흡착제 생산은 기초배지에서 보다 약 3배 증가하였다. Jar fermentor 배양에서 배양 60시간에서 가장 많은 흡착제를 생산하였으며, 흡착제의 생산량은 9.2 g/l 이었다. A strain (designated SK31) which produces an excellent adsorption substance was isolated from soil samples and identified as Bacillus species. The major adsorption substance (biosorbent SK31) produced by Bacillus sp. SK31 was purified by ethanol precipitation and cetylpyridinium (CPC) precipitation. The adsorption charactics of zinc and lead on bioadsorbent SK31 were investigated. The equilibrium isotherms showed that bioadsorbent SK31 took up zinc and lead from aqueous solutions to the extent of about 52 mg/g and 112 mg/g. respectively. The production of polysaccharide bioadsorbent, SK31. The optimum pH and temperature for sorbent production were 7.5 and 30℃, respectively. The important carbon and nitrogen sources for sorbent formation were glucose and ammonium nitrate, respectively. In the optimized medium, sorbent production was improved three folds in comparison with the basal medium. In the jar fermenter, the highest sorbent production was obtained at 60 h cultivation time and the amount of biosorbent SK31 at that time was 9.2 g/l.

Bacillus sp. SY12을 이용한 생물응집체 생산

서현효,정병윤,김형갑 진주산업대학교 환경문제연구소 1998 環境硏究論文集 Vol.2 No.-

A gram(+) bacteria that produced microbial flocculant was isolated from soil and classified as a Bacillus species and named as Bacillus sp. SY12. The culture conditions of the strain for flocculant production were studied in a shake flask. Optimum temperature and initial pH for flocculant production were 30℃ and 6.5, respectively. The optimized medium has following composition : glucose 20 g/ℓ, NH_4NO_3 0.5 g/ℓ, K_2HPO_4 1.0 g/ℓ, KH_2PO_4 0.8 g/ℓ, MgSO_4·7H_2O 0.5 g/ℓ, MnSO_4·4H_2O 0.3 g/ℓ, CaCO_3 0.5 g/ℓ, yeast extract 0.3 g/ℓ in tap water. Under the optimum culture conditions, flocculant production was improved by ten times in comparison with in basal medium. In the jar fermentor, the highest flocculating activity was obtained at 30-h cultivation time and the flocculant was increased with the increase of cells.

미생물 유래 β-glucan 분리 · 정제 및 이를 이용한 오염물질 정화용 복합 미생물제재 개발

서현효,이현돈,김형갑 진주산업대학교 산업과학기술연구소 2007 산업과학기술연구소보 Vol.- No.14

β-glucan occuring mainly in mushrooms and fungi are attractive candidates for numerous industrial application due to their pharmacological activity such as antitumor activity via immunomodulating behavior and excellent rheological properties such as emulsification, viscosity, jellation, etc. Screening of β-glucan producing microorganism was performed by congo red dye binding assay of ethanol-precipitates from each culture filtrate. The β-glucan produced form the selected strains was purified mainly by directly diluting the culture broth with the same volume of water, removal of cells by centrifugation at 4,000×g for 20 mim, and precipitation with 3 volumes of ethanol. The monosaccharide composition was analyzed by either TLC or HPLC afer trifuoroacetic acid hydrolysis. The molecular mass of the β-glucan of HH-71 strain was estimated to be about 4,500,000 dalton on a GFC-HPLC chromatogram. A Bacillus protease coated with β-glucan showed 3-times more increased thermal stability comparing to the uncoated control when stored at 30℃ for 1 week. In a similar manner coating of wastewater (containing chlorinated organic compounds) treatment bacteria with β-glucan resulted in significant increase of thermal stability. Although more detailed experimental data need to be accumulated, these preliminary data clearly suggest that β-glucan is applicable as a coating material to stabilize activity or viability of industrial enzymes and microorganisms for treatment of wastewater. Based on these results, β-glucans obtained from HH-71 and HH-87 strains are currently investigated for coating ablility of microbial consortia for the treatment of wastewater.

제지폐수 처리용 미생물 제제 개발

서현효,김형갑 진주산업대학교 농업기술연구소 2000 農業技術硏究所報 Vol.13 No.-

This study presents the effects of microbial augmentation on treatment of paper mill wastewater. A strain SJ7 which produces an extracellular cellulase was isolated from samples of sawdust and soil. Bases on the morphological and physiological characteristics, the isolate SJ7 was identified as Pseudomonas sp. and named Pseudomonas sp. SJ7. Microbial augmentation CE-1 is a special blend of bacterial cultures(Nasseria sp. S4, Bacillus sp. JB2, Corynebacterium murium BY9, Pseudomonas sp. SJ7) selected and adapted to improve their ability to treat paper mill wastewater. For the evaluation of industrial applicability of microbial augmentation CE-1, treatment efficiency was examined using paper mill wastewater. The reducing rate of chemical oxygen demand(COD) and biochemical oxygen demand(BOD) by CE-1 against paper mill wastewater was 84.5% and 91.8%, respectively.

Bacillus sp. A56을 이용한 응집제 생산

서현효,이문호,김희식,박찬선,윤병대 한국산업미생물학회 1993 한국미생물·생명공학회지 Vol.21 No.5

토양에서 분리된 응집제 생산균주 A56은 gram 양성균이고, 운동성을 가진 간균으로 Bacillus sp.에 속하는 균주로 동정되어 최종적으로 Bacillus sp. A56으로 명명하였다. Bacillus sp. A56의 응집제 생산을 위한 최적 배양온도 및 초기 pH는 각각 30℃와 pH 6.5로 나타났으며, 탄소원과 질소원으로써는 glucose와 NH_4NO_3가 첨가된 배지에서 가장 높은 응집활성을 보였다. 유기질소원인 yeast extract와 tryptone의 경우 1 : 1의 비율인 0.03%씩을 각각 첨가했을 때 응집활성과 균체증식을 향상시키는 것으로 나타났다. 한편, CaCO_3와 MnSO_4도 응집활성을 높이는데 중요한 성분으로 작용하였으며, MgSO_4·7H_2O는 0.02% 농도 이상에서는 응집제 생산을 저해하는 것으로 나타났다. 응집제 생산을 위한 최적 C/N비(glucose/NH_4NO_3)는 2%(w/v) glucose 농도에서 40이었으며, 최적 배양 조건에서의 응집활성은 기초배지에서 보다 약 10배 정도 향상되었다. 또, jar fermentor 배양에서는 flask 배양시보다 배양시간이 단축되어 배양 30시간에 최고의 응집활성을 보였으며, 응집활성은 1.5배 증가하였다. A gram(+) bacteria that produced microbial flocculant was isolated from soil and classified as a Bacillus species and named as Bacillus sp. A56. The culture conditions of the strain for flocculant production were studied in a shake flask. Optimum temperature and initial pH for flocculant production were 30℃ and 6.5, respectively. The optimized medium has following composition: glucose 20 g/ℓ, NH_4NO_3 0.5 g/ℓ, K_2HPO_4 1.0 g/ℓ, KH_2PO_4 0.8 g/ℓ, MgSO_4 ·7H_2O 0.2 g/ℓ, MnSO_4·4-6J_2O 0.3 g/ℓ, CaCO_3 0.5 g/ℓ, yeast extract 0.3 g/ℓ, tryptone 0.3 g/ℓ in tap water. Under the optimum culture conditions, flocculant production was improved by ten times in comparison with in basal medium. In the jar fermentor, the highest flocculating activity was obtained at 30-hr cultivation time and the flocculant was increased with the increase of cells.

원유 분해균주의 분리 및 특성

서현효,김형갑 진주산업대학교 환경문제연구소 1998 環境硏究論文集 Vol.2 No.-

Bacterial strains which degrade crude oil were isolated by liquid culture from oil-spilled soil, and four isolates were selected among them. The strain H17-1 was finally selected after testing emulsifying activity and oil conversion rate. The strain H17-1 was identified as a Norcardia sp. based on the test for morphological, biochemical and physiological characteristics. It appears to be highly specialized for growth on crude oil in minimal salts medium since it showed preference for oil or degradation products as substrates for growth. It was found that it could grow on at least fifteen different hydrocarbons. The optimum cultural and environmental conditions were seeked. Cell growth and emulsification activity as a function of time were also determined. Crude oil degradation and the reduction of product peak was identified by the analysis of remnant oil by gas chromatography after 3 days of cultivation. Approximately 83% of oil were converted into a form no longer extractable by organic solvents.

Screening of Alkaline lipase - producing Microorganisms

서현효,윤병대,안종석,이현선,정호권,민태익 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1992 한국미생물생명공학회 학술발표초록집 Vol.1992 No.1

Antioxidant and TNF-α Inducing Ability of Styela plicata Extracts

Hyun-Hyo Suh(서현효),Bo-Bae Lee(이보배),Seung-Cheol Lee(이승철) 한국생명과학회 2009 생명과학회지 Vol.19 No.4

메탄올, 에탄올, 아세톤 그리고 물을 이용하여 신선한 오만둥이의 추출물을 제조한 후, 추출물의 항산화능 및 TNF-α 유도능을 조사하였다. 아세톤 추출물을 제외한 대부분의 추출물은 malonedialdehyde 생성을 50% 이상 저해하는 항산화능을 보였으며, 메탄올 추출물은 가장 높은 값인 59.3%의 malonedialdehyde 생성 저해능을 나타내었다. 물과 아세톤 추출물은 각각 18.5와 12.3 μM의 glutathione 함량을 보였지만 그 외의 추출물에서는 검출되지 않았다. 추출물들의 TNF-α 유도능을 RAW 264.7 세포주로 조사하였다. 오만둥이 물 추출물은 첨가량이 증가할수록 TNF-α 생성을 증가시켰다. 그러나 그 이외의 추출물들은 RAW 264.7 세포주에서의 증가할수록 TNF-α 생성을 유도하지 못하였다. After preparing extracts from fresh Styela plicata using methanol, ethanol, acetone, and water, the antioxidant and tumor necrosis factor (TNF)-α inducing ability of the extracts were evaluated. Most of the extracts showed more than 50% inhibitory activity against malonedialdehyde formation, and methanol extract exhibited the highest activity with values of 59.3%. The water and acetone extracts contained glutathione contents of 18.5 and 12.3 μM, respectively, however, these were not detected in the other extracts. TNF-α inducing abilities of the extracts were determined with RAW 264.7 cells. The water extract increased TNF-α production with correlation to increase of the added amount. However, the other extracts could not induce TNF- α production in RAW 264.7 cells. The results indicated that the antioxidant activities of S. plicata extracts were different depending on extracting solvents, and the water extract showed significant TNF-α inducing activity.

비화음 및 침치료로 호전된 3세대 퀴놀론계 항생제 유발 소화장애 치험례

서현식,정진용,손창규,이남헌,조정효,Seo, Hyun-Sik,Joung, Jin-Yong,Son, Chang-Gue,Lee, Nam-Hun,Cho, Jung-Hyo 대전대학교 한의학연구소 2017 한의학연구소 논문집 Vol.26 No.1

Objectives: The purpose of this case study is to report the clinical effects of acupuncture and Korean medicine on antibiotics-induced functional dyspepsia. Methods: The patient, 79-year-old, who complained about nausea and vomiting after taking antibiotics was treated by acupuncture and Korean medicine. We measured the amount of meals eaten by patient and quantified the degree of nausea she felt. Results: After the treatment, the symptoms were decreased. Conclusion: Acupuncture and herb medicine may be effective therapy on antibiotic's side effects such as nausea and vomit.

Bowel Perforation Treated with Acupuncture and Gami-Gamchogungang-tang : A Case Report

서현식,김준열,주한음,조영민,배혜리,조정효 대한한방내과학회 2023 大韓韓方內科學會誌 Vol.44 No.4

Objective: This case report details the successful management of bowel perforation through traditional Korean medicine. Often, emergency surgery is required due to potential complications, such as peritonitis. In this case, the patient had previously undergone a total colectomy, making surgical treatment complicated. Methods: The patient revealed persistent abdominal pain and over 20 instances of diarrhea per day. During the course of treatment, which included two hospitalizations and one outpatient visit, acupuncture treatment and herbal medicine were administered. Throughout the treatment period, the intensity of abdominal pain and the frequency of diarrhea gradually decreased. Results: At the end of treatment, a follow-up abdominal computed tomography (CT) scan showed no evidence of perforation. Additionally, blood tests revealed no abnormalities in liver or kidney function, confirming the safety of the treatments.