혈관평활근세포에서 산화에너지대사 억제에 의한 아밀로이드전구단백질 대사의 변화

한문구,최웅,김헌식,안희열,한설희 대한치매학회 2002 Dementia and Neurocognitive Disorders Vol.1 No.1

Background: A reduction in the activity of cytochrome c oxidase(COX) has been recently identified in mitochondria from platelets and postmortem brain tissue of AD patients Sodium azide (NaN₃). a COX inhibitor, is an effective chemical agent producing energy shortage and oxidative stress both in vitro and in vivo system Furthermore it has been suggested that vascular compromise could be either directly involved AD pathogenesis or indirectly associated with triggering pathogenetic events leading to AD This study was performed to investigate amyloid precursor protein (APP) metabolism by inhibition of mitochondrial energy metabolism in cultured vascular smooth muslce cells (VSMCs) Materials and Methods: VSMCs isolated from the aorta of seven weeks old Spraque-Dawley rat were treated with NaN₃in a low concentration (100-500μM) or in a high concentration (1-100mM) Cellular proliferation and viability were determined by MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenly)-2H-tetrazolium) assay Cellular APP was detected with N-terminal specific antibody 22C11. Celldeath was determined by observation of morphology and terminal deoxynucleotidyl transferase-mediated dUTP-fluorescein nick end labeling stain (TUNEL) We used ginkgo biloba extract(EGb761) and melatonin as anti-oxidants to investigate the mechanism of latered APP metabolism Results: The viability of VSMCs was increased after treatment with 1 mM and 10mM NaN₃(p<0.05) unitl 3 hr and then dimnished Many TUNEL positive cells were found in 10mM and 100mM treatment group. but were not apoptotic in nature 22C11 immunoreactivity was not changed at 3 hr, 6hr, 12hr Anti-oxidants reduced cellular proliferation (p<0.05). but did not block TUNEL positivities and did not influence the 22C11 immunoreactivity In a low concentration NaN₃ treatment group the viability of VSMCs was increased concentration dependently(p<0.05) Immunoblot with 22C11 showed the concentration dependent decrease at 145 kDa, 125 kDa. and high molecular weight range (>160kDa) TUNEL staining showed DNA fragmentations and condensations of nuclear chromatin suggesting apoptosis After treatment with anti-oxidants, the cellular proliferation was more decreased (p<0.05), and TUNEL positive cell deaths were blocked Immunoreactivities of 125 kDa (immature APP). 145 kDa (mature APP). and higher molecular weight bands were recovered below 400μM of NaN₃ Immunoreactivity of 145 kDa was recovered in 100 μM NaN₃ treated group Conclusions: The presumed mechanism of low concentration COX inhibitor is the overproduction of reactive oxygen species resulting from a depression of the mitochondiral electron transport chain. whereas potential consequence of high concentration COX inhibitor might be related to decreassion of ATP synthesis and bioenergetic impairment Reactive oxygen radicals in response to low concentration COX inhibitor alter the processing of APP in VSMCs This investigation demonstrated analtered APP metabolism as a peripheral marker of AD Therefore VSMCs treated with low concentration COX inhibitor could be concsidered as a novel in vitro model of AD.

열공형과 비열공형 피질하 혈관성 치매에서 위험인자의 차이에 관한 비교 연구

배희준,정지향,유경호,나덕렬,김상윤,최경규,양동원,손의주,이상도,김재우,박경원,김응규,이재홍,박미영,한일우,함동석,최문성,하충건,최성혜,이애영,이병철,한설희 대한치매학회 2003 Dementia and Neurocognitive Disorders Vol.2 No.2

Backgrounds and Objectives: Vascular dementia is a group of dementing disoders arising from various stroke syndrome. Among these. subcortical ischemic vascular dementia (SIVD) is regarded as a relatively distinct clinical entity. However, MRI patterns of SIVD are not homogenous. In some patients, lacunes are dominant, and in others, subcortical white matter changes are. This study was designed to compare risk factor profiles between SIVD with and without multiple lacunes. Methods: We divided 47 subjects (22 males, mean age. 68 years) recruited from VADAPET (Multicenter Trial For Evaluation Of The Changes In the PET Images Of Subcortical Vascular Dementia Patient) study into two groups one with more than 5 lacunes in deep gray matter (lacune group) and the other with 5 or less(non-lacune group) Clinical characteristics and laboratory findings of two groups were compared. Results: Nineteen of 47 patients (40%) belonged to the lacune group. The lacune and non-lacune groups d d not differ in the following variables: age, hypertension, diabetes mellitus, hyperlipidemia heart disease, history of stroke or TIA, history of trauma or major surgery, family history of hypertension stroke, or dementia, age at diagnosis of dementia, body mass index, white blood cell count, ESR, CRP, fibrinogen, hemoglobin A1C, total cholesterol. LDL cholesterol creatinine, proteinuria, glucosuria, and microhematuria. However, male sex, smoking alcohol. hemoglobin, and HDL cholesterol were possibly associated more with lacune group SIVD than with non-lacune group (p<0 1) Multivariate analyses revealed that smoking, hemoglobin, and HDL cholesterol were independent predictors of SIVD with multiple lacunes Conclusion: Our study suggests that SIVD with multiple lacunes may be significantly different in smoking habits hemoglobin, and HDL cholesterol from SIVD without multiple lacunes.

(AgCu)_0.43-Au_0.54Pd_0.03 합금의 시효경화 특성

배동화,설효정,이희경,김형일,김교한 대한치과기재학회 2001 대한치과재료학회지 Vol.28 No.3

팔라디움이 함유된, 시효경화성 치과주조용 고금합금의 연구를 위한 기초자료를 얻기 위하여, 4원계 □ 합금의 등온시효경화 거동과 상변태를 경도 시험, X선회절 실험으로 조사하여, 다음과 같은 결과를 얻었다. 본 합금을 250~350℃에서 등온시효처리하면, 면심정방구조를 갖는 준안정상 AuCu Ⅰ'형 규칙상이 면심정방구조를 갖는 AuCu Ⅰ형 규칙상과 면심입방구조를 갖는 Ag-rich a₁상이 생성되기 전에 생성되었다. 본 합금은 시효처리 온도에 따라 다른 시효경화 거동을 보여주었다. 350℃에서 시효처리를 하면, 경도는 시효 초기에 상승하지 않았고, 최고 경도에 도달하여 유지되고나서 시효시간을 연장함에 따라 경도가 급격히 저하하였다. 250℃에서 시효처리를 하면, 경도는 시효 초기부터 뚜렷히 상승하기 시작하였다. 그 후, 약간의 경도 하강을 보이고나서 경도는 다시 서서히 상승을 지속하였다. 350℃에서 경화는 준안정상 AuCu Ⅰ'형 규칙상의 균일핵생성에 기인하였다. 낮은 시효온도에서의 초기 경화는 다른 상변태에 의해 초래되었고, 이어서 일어나는 경화는 준안정상 AuCu Ⅰ'형 규칙상의 생성에 기인하였다. 시효시간을 연장함에 따라 생기는 과시효 연화는 평형상 AuCu Ⅰ형 규칙상의 생성에 기인하는 것으로 생각된다.

Impact of COVID-19 Infection and Its Association With Previous Vaccination in Patients With Myasthenia Gravis in Korea: A Multicenter Retrospective Study

Han Hee Jo,Kim Seung Woo,Kim Hyunjin,So Jungmin,Lee Eun-Jae,Lim Young-Min,Lee Jung Hwan,Lee Myung Ah,Kim Byung-Jo,Baek Seol-Hee,Lee Hyung-Soo,Sohn Eunhee,Kim Sooyoung,Park Jin-Sung,Kang Minsung,Park H 대한의학회 2024 Journal of Korean medical science Vol.39 No.18

Background: During the coronavirus disease 2019 (COVID-19) pandemic, patients with myasthenia gravis (MG) were more susceptible to poor outcomes owing to respiratory muscle weakness and immunotherapy. Several studies conducted in the early stages of the COVID-19 pandemic reported higher mortality in patients with MG compared to the general population. This study aimed to investigate the clinical course and prognosis of COVID-19 in patients with MG and to compare these parameters between vaccinated and unvaccinated patients in South Korea. Methods: This multicenter, retrospective study, which was conducted at 14 tertiary hospitals in South Korea, reviewed the medical records and identified MG patients who contracted COVID-19 between February 2022 and April 2022. The demographic and clinical characteristics associated with MG and vaccination status were collected. The clinical outcomes of COVID-19 infection and MG were investigated and compared between the vaccinated and unvaccinated patients. Results: Ninety-two patients with MG contracted COVID-19 during the study. Nine (9.8%) patients required hospitalization, 4 (4.3%) of whom were admitted to the intensive care unit. Seventy-five of 92 patients were vaccinated before contracting COVID-19 infection, and 17 were not. During the COVID-19 infection, 6 of 17 (35.3%) unvaccinated patients were hospitalized, whereas 3 of 75 (4.0%) vaccinated patients were hospitalized (P < 0.001). The frequencies of ICU admission and mechanical ventilation were significantly lower in the vaccinated patients than in the unvaccinated patients (P = 0.019 and P = 0.032, respectively). The rate of MG deterioration was significantly lower in the vaccinated patients than in the unvaccinated patients (P = 0.041). Logistic regression after weighting revealed that the risk of hospitalization and MG deterioration after COVID-19 infection was significantly lower in the vaccinated patients than in the unvaccinated patients. Conclusion: This study suggests that the clinical course and prognosis of patients with MG who contracted COVID-19 during the dominance of the omicron variant of COVID-19 may be milder than those at the early phase of the COVID-19 pandemic when vaccination was unavailable. Vaccination may reduce the morbidity of COVID-19 in patients with MG and effectively prevent MG deterioration induced by COVID-19 infection.

Response of osteoblast-like cells cultured on zirconia to bone morphogenetic protein-2

Han, Seung-Hee,Kim, Kyoung-Hwa,Han, Jung-Seok,Koo, Ki-Tae,Kim, Tae-Il,Seol, Yang-Jo,Lee, Yong-Moo,Ku, Young,Rhyu, In-Chul Korean Academy of Periodontology 2011 Journal of Periodontal & Implant Science Vol.41 No.5

Purpose: The aim of this study was to compare osteoblast behavior on zirconia and titanium under conditions cultured with bone morphogenetic protein-2. Methods: MC3T3-E1 cells were cultured on sandblasted zirconia and sandblasted/etched titanium discs. At 24 hours after seeding MC3T3-E1, the demineralized bone matrix (DBM) gel alone and the DBM gel with bone morphogenetic protein-2 (BMP-2) were added to the culture medium. The surface topography was examined by confocal laser scanning microscopy. Cellular proliferation was measured at 1, 4, and 7 days after gel loading. Alkaline phosphatase activity was measured at 7 days after gel loading. The mRNA expression of ALPase, bone sialoprotein, type I collagen, runt-related transcription factor 2 (Runx-2), osteocalcin, and osterix were evaluated by real-time polymerase chain reaction at 4 days and 7 days. Results: At 1, 4, and 7 days after loading the DBM gel alone and the DBM gel with BMP-2, cellular proliferation on the zirconia and titanium discs was similar and that of the groups cultured with the DBM gel alone and the DBM gel with BMP-2 was not significantly different, except for titanium with BMP-2 gel. ALPase activity was higher in the cells cultured with BMP-2 than in the other groups, but there was no difference between the zirconia and titanium. In ALPase, bone sialoprotein, osteocalcin, Runx-2 and osterix gene expression, that of cells on zirconia or titanium with BMP-2 gel was much more highly increased than titanium without gel at day 7. The gene expression level of cells cultured on zirconia with BMP-2 was higher than that on titanium with BMP-2 at day 7. Conclusions: The data in this study demonstrate that the osteoblastic cell attachment and proliferation of zirconia were comparable to those of titanium. With the stimulation of BMP-2, zirconia has a more pronounced effect on the proliferation and differentiation of the osteoblastic cells compared with titanium.

[P10-35] Effect of Ulmi Cortex Extract on the Recovery of Immune Cells in Whole Body γ- Irradiated Mice

Seol-Hee Han,Hae-Ran Park,Uhee Jung,Sung-Kee Jo 한국식품영양과학회 2005 한국식품영양과학회 학술대회발표집 Vol.2005 No.10

Acute and 28-Day Subacute Toxicity Studies of Hexane Extracts of the Roots of Lithospermum erythrorhizon in Sprague-Dawley Rats

Han, Chung-Tack,Kim, Myoung-Jun,Moon, Seol-Hee,Jeon, Yu-Rim,Hwang, Jae-Sik,Nam, Chunja,Park, Chong-Woo,Lee, Sun-Ho,Na, Jae-Bum,Park, Chan-Sung,Park, Hee-Won,Lee, Jung-Min,Jang, Ho-Song,Park, Sun-Hee,H Korean Society of ToxicologyKorea Environmental Mu 2015 Toxicological Research Vol.31 No.4

Lithospermum erythrorhizon has long been used as a traditional oriental medicine. In this study, the acute and 28-day subacute oral dose toxicity studies of hexane extracts of the roots of L. erythrorhizon (LEH) were performed in Sprague-Dawley rats. In the acute toxicity study, LEH was administered once orally to 5 male and 5 female rats at dose levels of 500, 1,000, and 2,000 mg/kg. Mortality, clinical signs, and body weight changes were monitored for 14 days. Salivation, soft stool, soiled perineal region, compound-colored stool, chromaturia and a decrease in body weight were observed in the extract-treated groups, and no deaths occurred during the study. Therefore, the approximate lethal dose (ALD) of LEH in male and female rats was higher than 2,000 mg/kg. In the subacute toxicity study, LEH was administered orally to male and female rats for 28 days at dose levels of 25, 100, and 400 mg/kg/day. There was no LEH-related toxic effect in the body weight, food consumption, ophthalmology, hematology, clinical chemistry and organ weights. Compound-colored (black) stool, chromaturia and increased protein, ketone bodies, bilirubin and occult blood in urine were observed in the male and female rats treated with the test substance. In addition, the necropsy revealed dark red discoloration of the kidneys, and the histopathological examination showed presence of red brown pigment or increased hyaline droplets in the renal tubules of the renal cortex. However, there were no test substance-related toxic effects in the hematology and clinical chemistry, and no morphological changes were observed in the histopathological examination of the kidneys. Therefore, it was determined that there was no significant toxicity because the changes observed were caused by the intrinsic color of the test substance. These results suggest that the no-observed-adverse-effect Level (NOAEL) of LEH is greater than 400 mg/kg/day in both sexes.

한국 골프 도슨트 프로그램 적용 가능성에 대한 고찰

한설희(Han, Seol-Hee),김영재(Kim, Young-Jae) 한국체육과학회 2017 한국체육과학회지 Vol.26 No.4

In this study, an in-depth interview was conducted to hear about experience in participating in the golf docent program in detail. To achieve the study purpose, this study targeted 2 golf specialists out of 3 people including the researcher, who experienced the docent program at the President’s Cup in the Jack Nicklaus Golf Club of Songdo, Incheon on October 2015 and the following results were obtained. First, in relation to the title of the program, ‘Meet A Docent’, the two golf specialists replied that the docent program provided new experience, there were differences between golf commentary and the docent program, and the gallery showed satisfactory reaction. Second, in relation to the docent program as a new golf commentary, the gold specialists replied that it added realism to a golf commentary, the docent program provided a new event of golf, and qualifications for being a golf docent should be established. This study targeted two golf specialists with experience in working as docents for an in-depth interview. But the participants were limited and there was a limit in concrete analysis. Nevertheless, there were positive responses about the docent program in golf from the specialists and there should be continued studies on the golf docent program.

방사선 조사에 의한 방부제가 함유된 하이드로겔의 항균 효과

한설희 ( Seol Hee Han ),권희정 ( Hui Jeong Gwon ),임윤묵 ( Youn Mook Lim ),안성준 ( Sung Jun An ),윤민호 ( Min Ho Youn ),김해경 ( Hae Kyoung Kim ),문은경 ( Eun Gyeng Mun ),노영창 ( Young Chang Nho ) 한국공업화학회 2008 응용화학 Vol.12 No.2

상처 치료용 하이드로겔에 적용을 위한 방부제의 세포독성평가

한설희 ( Seol Hee Han ),권희정 ( Hui Jeong Gwon ),임윤묵 ( Youn Mook Lim ),안성준 ( Sung Jun An ),윤민호 ( Min Ho Youn ),조선영 ( Sun Young Jo ),문은경 ( Eun Gyeng Mun ),노영창 ( Young Chang Nho ) 한국공업화학회 2009 응용화학 Vol.13 No.1

Phenoxyethanol과 Paraben류는 항균제로서 제약과 화장품, 식품 안에 공통적으로 사용된다. 우리는 상처 치료용 겔의 부패 방지를 목적으로 phenoxyethanol, methyl paraben, propyl paraben 그리고 이들의 혼합물 사용을 위해 유효한 농도를 결정하고자 세포 독성 실험을 수행하였다. 세포독성 실험은 상기의 방부제를 mouse fibroblast cell line(L929)에 농도별로 처리하여 세포의 생존률로써 확인하였다. 이 결과를 바탕으로 phenoxyethanol, methyl paraben, propyl paraben 그리고 이들의 혼합물을 처리시 E.coli와 S.aureus의 성장 억제 활성을 확인하여 상처 치료용 겔에 적용하고자 한다. Phenoxyethanol and Paraben derivatives are well known as preservative and used in cosmetics and pharmaceutics and foods. Therefore, an investigation was carried out to assess the effect on the cytotoxicity of those preservatives when the preservatives were subjected to a commercial sterilization procedure using gamma-irradiation. The cytotoxicity tests of these hydrogels were evaluated by cell viability of L929 (mouse fibroblast). As results, the preservatives have a good cell viability results.