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Evaluation of Haemagglutinin Content by RP-HPLC to Generate Pandemic Influenza Vaccine
Kang, Hyunkyung,Roh, Hang Sik,Song, Hyemin,Lee, Kwangmoon,Chung, Seung-Tae,Ban, Sang-ja,Mo, In Pil,An, Beum-Soo,Ahn, Chi-Young Korean Society of ToxicologyKorea Environmental Mu 2016 Toxicological Research Vol.32 No.4
The potency of influenza vaccine is determined based on its hemagglutinin (HA) content. In general, single radial immunodiffusion (SRID) assay has been utilized as the standard method to measure HA content. However, preparation of reagents for SRID such as antigen and antibody takes approximately 2~3 months, which causes delays in the development of influenza vaccine. Therefore, quantification of HA content by other alternative methods is required. In this study, we measured HA contents of H1N1 antigen and H1N1 influenza vaccine by reverse phase-high performance liquid chromatography (RP-HPLC) methods. The presence of HA1 and HA2 was investigated by silver staining and Western blot assay. In addition, accuracy and repeatability of HA measurement by RP-HPLC were evaluated. Comparison of HA concentration by SRID and RP-HPLC revealed a precise correlation between the two methods. Our results suggest that RP-HPLC assay can replace SRID in the event of a pandemic flu outbreak for rapid vaccine development.
( Sang Mo Kang ),( Abdul Latif Khan ),( Young Hyun You ),( Jong Guk Kim ),( Muhammad Kamran ),( In Jung Lee ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.1
Very few plant growth-promoting rhizobacteria (PGPR) are known to produce gibberellins (GAs). The current study aimed to isolate a phytohormone-producing PGP rhizobacterium from soil and assess its potential to enhance plant growth. The newly isolated bacterium was identified as Leifsonia soli sp. SE134 on the basis of partial 16S ribosomal RNA gene sequence. Application of L. soli culture filtrate significantly increased the biomass, hypocotyl, and root lengths of cucumber seeds as compared with non-inoculated sole medium and distilled water treated controls. Furthermore, the PGPR culture was applied to the GA-deficient mutant rice cultivar Waito-C. Treatment with L. soli SE134 significantly increased the growth of Waito-C rice seedlings as compared with controls. Upon chromatographic analysis of L. soli culture, we isolated, detected and quantified different GAs; namely, GA1 (0.61 ± 0.15), GA4 (1.58 ± 0.26), GA7 (0.54 ± 0.18), GA8 (0.98 ± 0.15), GA9 (0.45 ± 0.17), GA12 (0.64 ± 0.21), GA19 (0.18 ± 0.09), GA20 (0.78 ± 0.15), GA24 (0.38 ± 0.09), GA34 (0.35 ± 0.10), and GA53 (0.17 ± 0.05). Plant growth promotion in cucumber, tomato, and young radish plants further evidenced the potential of this strain as a PGP bacterium. The results suggest that GA secretion by L. soli SE134 might prove advantageous for its ameliorative role in crop growth. These findings can be extended for improving the productivity of different crops under diverse environmental conditions.
S-728 Frequency of cancers in retroperitoneal fibrosis: a single center cohort
( Sang Jin Lee ),( Jung Su Eun ),( Eon Jeong Nam ),( Young Mo Kang ) 대한내과학회 2016 대한내과학회 추계학술대회 Vol.2016 No.1
Objectives: The objective of this study was to compare standardized incidence ratios (SIRs) of cancers in patients with retroperitoneal fibrosis (RPF) and age-sex matched general population. Methods: Medical records of 38 patients diagnosed at Kyung Pook National University Hospital as having RPF by computed tomography were reviewed. Thirteen cancer cases were identified in 38 RPF with patients. SIRs were calculated for total cancers and stratified according to cancer type, age at cancer diagnosis and RPF-cancer intervals compared to general population in korea. Results: RPF was significantly associated with malignant diseases (SIR 2.37, 95% CI 1.08 - 4.5), particularly other and unspecified urinary organ and thyroid cancers. The peak SIR was observed in the seventh decade of life (3.21, 1.04 - 7.5). When stratified by RPF-cancer intervals, SIR was 4.24 (1.71 - 8.74) within 2 years of RPF diagnosis, whereas no temporal relationship was found out of 2 years. Malignancies (n=7) within 2 years of RPF diagnosis were lung cancer (n=2), rectal cancer (n=1), gallbladder cancer (n=1), pancreatic cancer (n=1), thyroid cancer (n=1) and papillary urothelial cancer (n=1). Most of these was adenocarcinoma (n=5). Conclusion. RPF was strongly associated with cancers and most cancers developed within 2 years of RPF diagnosis. Our results indicate that cancer screening in patients with RPF may be performed regularly up to 2 years after RPF diagnosis.