Regulation of Gut Microbiota Disrupts the Glucocorticoid Receptor Pathway and Inflammation-related Pathways in the Mouse Hippocampus

Xuechen Rao,Lanxiang Liu,Haiyang Wang,Ying Yu,Wenxia Li,Tingjia Chai,Wei Zhou,Ping Ji,Jin-Lin Song,Hong Wei,Peng Xie 한국뇌신경과학회 2021 Experimental Neurobiology Vol.30 No.1

An increasing number of studies have recently indicated the important effects of gut microbes on various functions of the central nervous system. However, the underlying mechanisms by which gut microbiota regulate brain functions and behavioral phenotypes remain largely unknown. We therefore used isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomic analysis to obtain proteomic profiles of the hippocampus in germ-free (GF), colonized GF, and specific pathogen-free (SPF) mice. We then integrated the resulting proteomic data with previously reported mRNA microarray data, to further explore the effects of gut microbes on host brain functions. We identified that 61 proteins were upregulated and 242 proteins were downregulated in GF mice compared with SPF mice. Of these, 124 proteins were significantly restored following gut microbiota colonization. Bioinformatic analysis of these significant proteins indicated that the glucocorticoid receptor signaling pathway and inflammation-related pathways were the most enriched disrupted pathways. This study provides new insights into the pathological mechanisms of gut microbiota-regulated diseases.

GKAP, a Novel Synaptic Protein That Interacts with the Guanylate Kinase-like Domain of the PSD-95/SAP90 Family of Channel Clustering Molecules

Kim, Eunjoon,Naisbitt, Scott,Hsueh, Yi-Ping,Rao, Anuradha,Rothschild, Adam,Graig, Ann Marie,Sheng, Morgan 부산대학교 유전공학연구소 1997 분자생물학 연구보 Vol.13 No.-

The molecular mechanisms underlying the organization of ion channels and signsling molecules at the synaptic junction are largely unknown. Recently, members of the PSD-95/SAP90 family of synaptic MAGUK(menbrane-acssociated guanylate kinase) proteins have been shown to interact. via their NH_2-terminal PDZ domains, with certain ion channels(NMDA receptors and K^+channels). thereby promoting the clustering of these proteins. Although the function of the NH_2-terminal PDZ domains in relatively well characterized, the function of the Src homology 3(SH3) domain and the guanylate kinase-like(GK)domain in the COOH-terminal half of PSD-95 has remained obscure. WE now repoet the isolation of a novel synaptic protein. termed GKAP for guanylate kinase-associated protein. that binds directly to the GK domain of the known members of the mammalian PSD-95 family. GKAP shows a unique domain structure and appear to be a major constituent of the postsynaptic density. GKAP colocalizes and coimmunoprecipitates with PSD-95 in vivo,and colusters with PSD-95 and K^+ channels/parent lack of guanylate kinase enzymatic activity, the fact that the GK domain can as a site for protein-protein interaction has implications for the function of diverse GK-containing proteins(such as p55,ZO-1,and LIN-2/CASK).

Accuracy Analysis for 3D Model Measurement Based on Digital Close-range Photogrammetry Technique for the Deep Foundation Pit Deformation Monitoring

Lei-lei Guan,Yong-gui Chen,Rao-ping Liao 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.2

The deformation of deep foundation pits (DFP) may pose a huge potential threat to the safety of the bracing system and the surrounding environment. A large amount of deformation data facilitates the deformation prediction of DFP and the dynamic adjustment of the bracing scheme. This study proposes and validates a 3D modeling technique based on digital close-range photogrammetry, which can obtain sufficient deformation data and enhance the understanding of deformation patterns. Two typical sets of deformation experiments, namely the vertical deformation test and the horizontal deformation test, were conducted to examine the viability and accuracy of 3D model measurement. Furthermore, the factors affecting the accuracy of the measurement were discussed in detail. The results indicate that the measurement accuracy of 3D models can reach millimeter-level or even sub-millimeter level. The measurement accuracy is affected by the coupling of the pixel resolution of CCD, focal length, photographic distance, and photographic baseline. The increase in the pixel resolution of CCD and focal length improve the measurement accuracy of 3D model measurement, but increasing photographic distance shows the opposite trend. The ratio of photographic baseline to photographic distance (BDR) has a much greater effect on accuracy than other factors. The photographic distance with a resolution of less than 1.0mm and the BDR value of 0.6 − 1.8 are recommended. Based on the results, it is found that this technique can be adopted as an effective monitoring method in DFP deformation monitoring due to the high precision and labor-saving.

Convenient hydrogel adhesion with crystalline zones

Wen-Tao Chen,Liangpeng Zeng,Ping Li,Yi Liu,Jing-Liang Huang,Hui Guo,Ping Rao,Wei-Hua Li 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.117 No.-

Adhering soft hydrogels to hard substrates is challenging. Existing adhesion approaches mainly rely oninterfacial chemical bonds or physical interactions, which typically require complex fabrication processesor specific surface properties. Herein, we report a convenient approach to achieving effective hydrogeladhesion onto various hard substrates with in-situ formed crystalline zones by heating. Semicrystallizablepoly(vinyl alcohol) hydrogels were directly cast on various subtracts by a dryingswellingprocess, which demonstrates strong adhesion with adhesion energy as high as 890 J/m2. Theadhesion performance demonstrates an obvious positive correlation with the crystalline property. Therefore, the factors determining the crystalline degree, including the polymer type and preparationtemperature, demonstrate remarkable influences on the adhesion performance. Moreover, this adhesionperformance manifests high durability in harsh environments. Benefitting from the strong and durableadhesion performance, the crystalline gels can serve as a tie coating layer to joint various hydrogels,which demonstrate satisfactory antifouling ability. Therefore, this strategy may endow the materials withgreat potential for various practical applications in the future.

Two distinct types of dust polarization in the disk and its vicinity around the protostar TMC-1A

Yusuke Aso,Woojin Kwon,Tao-Chung Ching,Shih-Ping Lai,Zhi-Yun Li,Naomi Hirano,Ramprasad Rao 한국천문학회 2020 天文學會報 Vol.45 No.1

Pentacene as a hole transport material for high performance planar perovskite solar cells

Xiude Yang,Gang Wang,Debei Liu,Yanqing Yao,Guangdong Zhou,Ping Li,Bo Wu,Xi Rao,Qunliang Song 한국물리학회 2018 Current Applied Physics Vol.18 No.10

A cost-effective and efficient organic semiconductor pentacene was developed as a hole transport layer (HTL) material to replace classical PEDOT:PSS for planar perovskite solar cells (PSCs). As expected, the pentacene based device exhibits power conversion efficiency (PCE) of 15.90% (Jsc of 19.44 mA/cm2, Voc of 1.07 V, and FF of 77%), comparable to the PEDOT:PSS based device (PCE of 15.65%, Jsc of 18.78 mA/cm2, Voc of 1.07 V, and FF of 77%) under the same experimental conditions. The excellent performance of vacuum deposited pentacene is mainly attributed to the high efficient charge extraction and transfer in device due to the high-quality perovskite film grown on the top of pentacene substrate and a favorable energy-level alignment together with a desired downward band bending formed at the perovskite/pentacene interface. Our research has confirmed that pentacene could be served as a promising HTL material to achieve effective and potentially economical planar type PSCs.

NEDD4L suppresses PD-L1 expression and enhances anti-tumor immune response in A549 cells

Zhong Bin,Zheng Jie,Wen Huilan,Liao Xinhui,Chen Xingxiang,Rao Yunwei,Yuan Ping 한국유전학회 2022 Genes & Genomics Vol.44 No.9

Background: Non-small cell lung cancer (NSCLC) poses a salient threat to public health. E3 ubiquitin ligase commonly functions as an anti-tumor role. Objective: This study probed the effect of E3 ligase NEDD4L on A549 cells. Methods: NEDD4L expression in NSCLC and its correlation with NSCLC patient's prognosis were predicted and verified. PD-L1 protein level was measured, and the correlation between NEDD4L and PD-L1 was analyzed. The effects of NEDD4L overexpression on the binding of NEDD4L to PD-L1 and ubiquitination level of PD-L1 were examined. Xenograft tumor model was established in mice. The volume and weight of xenograft tumors were recorded. The proportion of CD8+ T cells and contents of IL-2 and INF-γ were detected. Results: NEDD4L expression was downregulated in NSCLC tissues and A549 cells, and correlated with poor prognosis of NSCLC patients. PD-L1 was upregulated in NSCLC and negatively correlated with NEDD4L. Overexpression of NEDD4L upregulated ubiquitination level of PD-L1 and reduced protein level of PD-L1. Overexpression of NEDD4L decreased tumor volume and weight and enhanced proportion of CD8+ T cells and contents of IL-2 and INF-γ. Conclusions: Collectively, overexpression of NEDD4L suppressed PD-L1 protein level through ubiquitination, thereby enhancing anti-tumor immune response and retarding NSCLC progression.