Effects of Celecoxib on Cycle Kinetics of Gastric Cancer Cells and Protein Expression of Cytochrome C and Caspase-9

Wang, Yu-Jie,Niu, Xiao-Ping,Yang, Li,Han, Zhen,Ma, Ying-Jie Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.4

Objective: This investigation aimed to determine effects of celecoxib on the cell cycle kinetics of the gastric cancer cell line MGC803 and the mechanisms involved by assessing expression of cytochrome C and caspase-9 at the protein level. Methods: Cell proliferation of MGC803 was determined by MTT assay after treatment with celecoxib. Apoptosis was assessed using fluorescence staining and cell cycle kinetics by flow cytometry. Western blotting was used to detect the expression of caspase-9 protein and of cytochrome C protein in cell cytosol and mitochondria. Results: Celecoxib was able to restrain proliferation and induce apoptosis in a dose- and time-dependent manner, inducing G0/G1 cell cycle arrest, release of cytochrome C into the cytosol, and cleavage of pro-caspase-9 into its active form. Conclusion: Celecoxib can induce apoptosis in MGC803 cells through a mechanism involving cell cycle arrest, mitochondrial cytochrome C release and caspase activation.

Fermented cottonseed meal improves production performance and reduces fat deposition in broiler chickens

Niu Jun Li,Wei Lian Qing,Luo Yuan Qing,Yang Wen Ting,Lu Qi Cheng,Zheng Xin Xia,Niu Yu Jie,Sheng Wen,Cheng Hong,Zhang Wen Ju,Nie Cun Xi 아세아·태평양축산학회 2021 Animal Bioscience Vol.34 No.4

Objective: This study was conducted to investigate the effect of fermented cottonseed meal (FCSM) on growth performance, carcass traits, and fat deposition in white-feather broiler chickens. Methods: A total of 480 male one-day-old white-feather broiler chickens were selected randomly and divided into four groups with six replicates of 20 chickens in each. The experimental chickens were fed diets including 3%, 6%, or 9% FCSM fermented by Candida tropicalis until 42 days old. In the experiment, the chickens of the control group were fed soybean meal. Results: FCSM supplementation linearly decreased the feed conversion ratio from d 15 to 21 and d 36 to 42, respectively (p<0.05). The percentage of carcass and semi-eviscerate increased in response to dietary FCSM supplementation at d 21 (p<0.05). The percentage of eviscerated and semi-eviscerate of 3FCSM was higher than that in other groups at d 35 (p<0.05). At the age of 42 d, the percentage of carcass increased in a quadratic way among increasing FCSM in diets (p<0.05). The subcutaneous fat thickness linearly decreased with the increasing levels of FCSM at d 21 (p<0.05). Gompertz and Logistic functions provided a better fit on abdominal fat and subcutaneous fat, respectively. The best fitted equation predicted that the maximum growth rate of abdominal fat weight and subcutaneous fat thickness occurred at d 28. FCSM had no significant effects on the shape of growth curve of abdominal fat weight and subcutaneous fat thickness, but reduced the height of the curve. Birds receiving the 6FCSM diet for 21 d had smaller adipocyte surface and lower serum glucose as well as triglyceride concentration. Conclusion: FCSM is beneficial for broiler chickens as it positively affects their growth and carcass in addition to altering their fat deposition. Objective: This study was conducted to investigate the effect of fermented cottonseed meal (FCSM) on growth performance, carcass traits, and fat deposition in white-feather broiler chickens.Methods: A total of 480 male one-day-old white-feather broiler chickens were selected randomly and divided into four groups with six replicates of 20 chickens in each. The experimental chickens were fed diets including 3%, 6%, or 9% FCSM fermented by <i>Candida tropicalis</i> until 42 days old. In the experiment, the chickens of the control group were fed soybean meal.Results: FCSM supplementation linearly decreased the feed conversion ratio from d 15 to 21 and d 36 to 42, respectively (p<0.05). The percentage of carcass and semi-eviscerate increased in response to dietary FCSM supplementation at d 21 (p<0.05). The percentage of eviscerated and semi-eviscerate of 3FCSM was higher than that in other groups at d 35 (p<0.05). At the age of 42 d, the percentage of carcass increased in a quadratic way among increasing FCSM in diets (p<0.05). The subcutaneous fat thickness linearly decreased with the increasing levels of FCSM at d 21 (p<0.05). Gompertz and Logistic functions provided a better fit on abdominal fat and subcutaneous fat, respectively. The best fitted equation predicted that the maximum growth rate of abdominal fat weight and subcutaneous fat thickness occurred at d 28. FCSM had no significant effects on the shape of growth curve of abdominal fat weight and subcutaneous fat thickness, but reduced the height of the curve. Birds receiving the 6FCSM diet for 21 d had smaller adipocyte surface and lower serum glucose as well as triglyceride concentration.Conclusion: FCSM is beneficial for broiler chickens as it positively affects their growth and carcass in addition to altering their fat deposition.

A New Analytic Solution to Determine Internal Load of Small Span Suspension Bridge

Wen-jie Niu,Hai-tao Yu 대한토목학회 2016 KSCE JOURNAL OF CIVIL ENGINEERING Vol.20 No.4

Until now, no available analytic solution considering suspender deformation was given to determine cable tension load at the middle of cable and suspender internal load of suspension bridge. Firstly, the classical theory without suspender deformation was taken to study the sag effect on the internal load of suspension bridge. Results indicate that the tension load at all cable points decreases when the sag increases. A procedure using Müller-Breslau principle to determine the moment and shear force influence line of the stiffening girder in the wire suspension bridge was given, and validated by the influence line given by the classical theory. Secondly, a new analytic solution considering suspender deformation was given to determine cable tension load at midpoint and the internal load of suspenders. The new analytic solution is more reasonable than the classical theory in theory. According to the new analytic solution, the cable tension load gets peak value when the unit live load Pi applies at the middle of stiffening girder, while the internal tension loads in all suspenders reach equal maximum value. Then a linear static model was built in ANSYS ignoring large displacement, initial strain and Ernst’s modulus of elasticity. Results using the new analytic solution compare well with benchmark simulations from ANSYS. So, the proposed analytic solution is a quick and easy way to approximately determine the internal load of small span suspension bridge.

An integrated strategy for production of four anthocyanin compounds from Ribes nigrum L. by deep eutectic solvents and flash chromatography

Ping Kou,Yu-Fei Kang,Li-Tao Wang,Li-Jun Niu,Ying Xiao,Na Guo,Qi Cui,Yanyan Liu,Yu-Jie Fu 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.80 No.-

This study explored an integrated strategy for extraction and preparative separation of four anthocyaninsfrom Ribes nigrum L. using DES-based microwave assisted extraction andflash chromatography. Theextraction yield of anthocyanins reached 2.03 mg/g after optimization. An efficient preparativeseparation process of four main anthocyanins by macroporous resin andflash chromatography wasestablished afterwards. The yields of D3G, D3R, C3G and C3R reached 0.32 mg/g, 0.47 mg/g, 0.18 mg/g and0.43 mg/g with the purities of more than 95%, respectively. The developed procedure represents anefficient and convenient alternative for the systematic utilization of natural products from plantmaterials.

TLF: Two-level Filter for Querying Extreme Values in Sensor Networks

( Min Meng ),( Jie Yang ),( Yu Niu ),( Young-koo Lee ),( Byeong-soo Jeong ),( Sungyoung Lee ) 한국정보처리학회 2007 한국정보처리학회 학술대회논문집 Vol.14 No.1

Sensor networks have been widely applied for data collection. Due to the energy limitation of the sensor nodes and the most energy consuming data transmission, we should allocate as much work as possible to the sensors, such as data compression and aggregation, to reduce data transmission and save energy. Querying extreme values is a general query type in wireless sensor networks. In this paper, we propose a novel querying method called Two-Level Filter (TLF) for querying extreme values in wireless sensor networks. We first divide the whole sensor network into domains using the Distributed Data Aggregation Model (DDAM). The sensor nodes report their data to the cluster heads using push method. The advantages of two-level filter lie in two aspects. When querying extreme values, the number of pull operations has the lower boundary. And the query results are less affected by the topology changes of the wireless sensor network. Through this method, the sensors preprocess the data to share the burden of the base station and it combines push and pull to be more energy efficient.

Diversity of bacterial community during ensiling and subsequent exposure to air in whole-plant maize silage

Hu, Zongfu,Chang, Jie,Yu, Jianhua,Li, Shuguo,Niu, Huaxin Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.9

Objective: To describe in-depth sequencing, the bacterial community diversity and its succession during ensiling of whole-plant maize and subsequent exposure to air. Methods: The microbial community dynamics of fermented whole-plant maize for 60 days (sampled on day 5, 10, 20, 40, 60) and subsequent aerobic exposure (sampled on day 63 after exposure to air for 3 days) were explored using Illumina Miseq sequence platform. Results: A total of 227,220 effective reads were obtained. At the genus level, there were 12 genera with relative abundance >1%, Lactobacillus, Klebsiella, Sporolactobacillus, Norank-c-cyanobacteria, Pantoea, Pediococcus, Rahnella, Sphingomonas, Serratia, Chryseobacterium, Sphingobacterium, and Lactococcus. Lactobacillus consistently dominated the bacterial communities with relative abundance from 49.56% to 64.17% during the ensiling process. Klebsiella was also an important succession bacterium with a decrease tendency from 15.20% to 6.41% during the ensiling process. The genus Sporolactobacillus appeared in late-ensiling stages with 7.70% abundance on day 40 and 5.32% on day 60. After aerobic exposure, the Lactobacillus decreased its abundance from 63.2% on day 60 to 45.03% on d 63, and Klebsiella from 5.51% to 5.64%, while Sporolactobacillus greatly increased its abundance to 28.15%. These bacterial genera belong to 5 phyla: Firmicutes (relative abundance: 56.38% to 78.43%) was dominant, others were Proteobacteria, Bacteroidetes, Cyanobacteria, and Actinobacteria. The bacterial communities clearly clustered into early-ensiling (d 5), medium-ensiling (d 10, d 20), late-ensiling (d 40, d 60), and aerobic exposure (d 63) clusters, with early- and late-ensiling communities more like each other than to the aerobic exposure communities. Conclusion: High-throughput sequencing based on 16S rRNA genes proved to be a useful method to explore bacterial communities of silage. The results indicated that the bacterial communities varied during fermentation and more dramatically during aerobic exposure. The study is valuable for understanding the mechanism of population change and the relationship between bacteria and ensilage characteristics.

Simulation Experiment on Acoustic Emission of Pipeline Leakage

Wang Xin-hua,Yang Jie,Jiao Yu-lin,Niu Yong-chao 보안공학연구지원센터 2015 International Journal of Smart Home Vol.9 No.2

With the heater pipeline leakage experiment conducted on the experiment table, inner pipeline leakage of heater was studied through the leakage failure detection system. The frequency distribution and the amplitude of acoustic emission signals of leakage versus internal pressure were analyzed. Variations of signals versus leakage aperture and dissemination distance were summarized. According to the mapping relationship between the leaking spot and acoustic emission signals, and between the leakage flow and acoustic emission signals, reasons of leakage failure were concluded. The results would be applied to heater leakage failure detection. And an on-the-spot detection in the power plant was conducted to test the heater under pressure in order to verify the results.

IL-11 promotes the treatment efficacy of hematopoietic stem cell transplant therapy in aplastic anemia model mice through a NF-κB/microRNA-204/thrombopoietin regulatory axis

Yan Wang,Zhi-yun Niu,Yu-jie Guo,Li-hua Wang,Feng-ru Lin,Jing-yu Zhang 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-

Hematopoietic stem cell (HSC) transplantation could be of therapeutic value for aplastic anemia (AA) patients, and immunosuppressants may facilitate the efficiency of the procedure. As anti-inflammatory cytokine interleukin-11 (IL-11) has a thrombopoietic effect, its use in cases of chronic bone marrow failure, such as AA, has been proposed to induce HSC function. However, the putative mechanisms that may support this process remain poorly defined. We found that decreased miR-204-5p levels were coincident with increased proliferation in mouse HSCs following exposure to IL-11 in vitro. Through inhibiting NF-кB activity, miR-204-5p repression was demonstrated to be a downstream effect of IL-11 signaling. miR-204-5p was shown to directly target thrombopoietin (TPO) via sequence-dependent 3′-UTR repression, indicating that this microRNA-dependent pathway could serve an essential role in supporting IL-11 functions in HSCs. Increased TPO expression in HSCs following IL-11 exposure could be mimicked or blocked by inhibiting or overexpressing miR-204-5p, respectively. Consistent with these in vitro findings, IL-11 promoted HSC engraftment in a mouse model of AA, an effect that was attenuated in cells overexpressing miR-204-5p. The reduction in miR-204-5p levels is an integral component of IL-11 signaling that may play an essential role in treating AA.

Online Leakage Detection Test of High-pressure Heater at the Power Plant

Wang Xin-hua,Jiao Yu-lin,Niu Yong-chao,Yang Jie 보안공학연구지원센터 2015 International Journal of Smart Home Vol.9 No.3

Variations of acoustic emission signals of leakage of the heater versus inner pressure, leakage aperture, transmission distance and liquid state in the outer pipeline were studied through experiment and analysis. By changing one of the four factors each time, leakage failure reasons were concluded. Research results would be applied into leakage detection of heater.

Single-cell RNA sequencing reveals B cell–related molecular biomarkers for Alzheimer’s disease

Xiong Liu-Lin,Xue Lu-Lu,Du Ruo-Lan,Niu Rui-Ze,Chen Li,Chen Jie,Hu Qiao,Tan Ya-Xin,Shang Hui-Fang,Liu Jia,Yu Chang-Yin,Wang Ting-Hua 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

In recent years, biomarkers have been integrated into the diagnostic process and have become increasingly indispensable for obtaining knowledge of the neurodegenerative processes in Alzheimer’s disease (AD). Peripheral blood mononuclear cells (PBMCs) in human blood have been reported to participate in a variety of neurodegenerative activities. Here, a single-cell RNA sequencing analysis of PBMCs from 4 AD patients (2 in the early stage, 2 in the late stage) and 2 normal controls was performed to explore the differential cell subpopulations in PBMCs of AD patients. A significant decrease in B cells was detected in the blood of AD patients. Furthermore, we further examined PBMCs from 43 AD patients and 41 normal subjects by fluorescence activated cell sorting (FACS), and combined with correlation analysis, we found that the reduction in B cells was closely correlated with the patients’ Clinical Dementia Rating (CDR) scores. To confirm the role of B cells in AD progression, functional experiments were performed in early-stage AD mice in which fibrous plaques were beginning to appear; the results demonstrated that B cell depletion in the early stage of AD markedly accelerated and aggravated cognitive dysfunction and augmented the Aβ burden in AD mice. Importantly, the experiments revealed 18 genes that were specifically upregulated and 7 genes that were specifically downregulated in B cells as the disease progressed, and several of these genes exhibited close correlation with AD. These findings identified possible B cell-based AD severity, which are anticipated to be conducive to the clinical identification of AD progression.