Association of Extracellular Cleavage of E-Cadherin Mediated by MMP-7 with HGF-Induced in vitro Invasion in Human Stomach Cancer Cells

Lee, K.H.,Choi, E.Y.,Hyun, M.S.,Jang, B.I.,Kim, T.N.,Kim, S.W.,Song, S.K.,Kim, J.H.,Kim, J.-R. S. Karger AG 2007 European surgical research Vol.39 No.4

<P><I>Background:</I> Proteolytic shedding of the ectodomain of a variety of transmembrane proteins, including cell-to-cell adhesion molecules, has been observed in solid cancers. We have investigated whether extracellular cleavage of E-cadherin mediated by matrix metalloproteinase-7 (MMP-7) is involved in hepatocyte growth factor (HGF) induced in vitro invasion in stomach cancer cells. <I>Methods:</I> The effects of HGF on the expression of E-cadherin/β-catenin and MMP-7 at both the protein and mRNA levels were assessed in stomach cancer cells, NUGC-3 and MKN-28, and in cells in which the expression of MMP-7 was downregulated by transfection with a MMP-7 short hairpin RNA plasmid. <I>Results:</I> Treatment with HGF increased the extracellular cleavage of E-cadherin and the release of MMP-7 and reduced the level of E-cadherin in a dose- and time-dependent manner. HGF treatment repressed the phosphorylation of β-catenin in a Triton-soluble fraction, but enhanced this phosphorylation in a Triton-insoluble fraction. The association of E-cadherin with β-catenin was decreased by HGF treatment in the Triton-soluble fraction. In addition, treatment of MMP-7 short hairpin RNA transfected NUGC-3 cells with HGF resulted in no extracellular cleavage of E-cadherin and also decreased the in vitro cell invasion. <I>Conclusions:</I> These results suggest that incubation with HGF mediated the release of MMP-7, resulting in extracellular cleavage of E-cadherin from stomach cancer cells. This might be a key mechanism in HGF-induced in vitro invasion and metastasis.</P><P>Copyright © 2007 S. Karger AG, Basel</P>

Direct analysis of site-specific N-glycopeptides of serological proteins in dried blood spot samples

Choi, N. Y.,Hwang, H.,Ji, E. S.,Park, G. W.,Lee, J. Y.,Lee, H. K.,Kim, J. Y.,Yoo, J. S. Springer Science + Business Media 2017 Analytical and Bioanalytical Chemistry Vol.409 No.21

<P>Dried blood spot (DBS) samples have a number of advantages, especially with respect to ease of collection, transportation, and storage and to reduce biohazard risk. N-glycosylation is a major post-translational modification of proteins in human blood that is related to a variety of biological functions, including metastasis, cell-cell interactions, inflammation, and immunization. Here, we directly analyzed tryptic N-glycopeptides from glycoproteins in DBS samples using liquid chromatography-tandem mass spectrometry (LC-MS/MS) without centrifugation of blood samples, depletion of major proteins, desalting of tryptic peptides, and enrichment of N-glycopeptides. Using this simple method, we identified a total of 41 site-specific N-glycopeptides from 16 glycoproteins in the DBS samples, from immunoglobulin gamma 1 (IgG-1, 10 mg/mL) down to complement component C7 (50 mu g/mL). Of these, 32 N-glycopeptides from 14 glycoproteins were consistently quantified over 180 days stored at room temperature. The major abundant glycoproteins in the DBS samples were IgG-1 and IgG-2, which contain nine asialo-fucosylated complex types of 16 different N-glycopeptide isoforms. Sialo-non-fucosylated complex types were primarily detected in the other glycoproteins such as alpha-1-acid glycoprotein 1, 2, alpha-1-antitypsin, alpha-2-macroglobulin, haptoglobin, hemopexin, Ig alpha 1, 2 chain C region, kininogen-1, prothrombin, and serotransferrin. We first report the characterization of site-specific N-glycoproteins in DBS samples by LC-MS/MS with minimal sample preparation.</P>

Analysis of fucosylation in liver-secreted N-glycoproteins from human hepatocellular carcinoma plasma using liquid chromatography with tandem mass spectrometry

Ji, E. S.,Hwang, H.,Park, G. W.,Lee, J. Y.,Lee, H. K.,Choi, N. Y.,Jeong, H. K.,Kim, K. H.,Kim, J. Y.,Lee, S. Springer Science + Business Media 2016 Analytical and Bioanalytical Chemistry Vol.408 No.27

<P>Fucosylation of N-glycoproteins has been implicated in various diseases, such as hepatocellular carcinoma (HCC). However, few studies have performed site-specific analysis of fucosylation in liver-secreted proteins. In this study, we characterized the fucosylation patterns of liver-secreted proteins in HCC plasma using a workflow to identify site-specific N-glycoproteins, where characteristic B- and/or Y-ion series with and without fucose in collision-induced dissociation were used in tandem mass spectrometry. In total, 71 fucosylated N-glycopeptides from 13 major liver-secreted proteins in human plasma were globally identified by LC-MS/MS. Additionally, 37 fucosylated N-glycopeptides were newly identified from nine liver-secreted proteins, including alpha-1-antichymotrypsin, alpha-1-antitrypsin, alpha-2-HS-glycoprotein, ceruloplasmin, alpha-1-acid glycoprotein 1/2, alpha-2-macroglobulin, serotransferrin, and beta-2-glycoprotein 1. Of the fucosylated N-glycopeptides, bi- and tri-antennary glycoforms were the most common ones identified in liver-secreted proteins from HCC plasma. Therefore, we suggest that this analytical method is effective for characterizing fucosylation in liver-secreted proteins.</P>

간호대학생의 전공만족도와 간호전문직관의 관계 : 일대학 간호대학생을 대상으로

고정은,김미희,김은진,김혜지,엄지은,이행아,장누리,장동은 이화여자대학교 간호학회 2011 이화간호학회지 Vol.- No.45

PURPOSE : This study purposed to examine the relation between nursing college students' major satisfaction and their nursing professionalism. METHOD : We collected data from 197 nursing students at a college in Seoul using a structured questionnaire from the 6th to 13th. The collected data was analyzed by SPSS 17.0. RESULT : 1. The mean score of major satisfaction was 3.54±49 and that of nursing professionalism was 3.52±46. Among the sub-areas of major satisfaction, satisfaction with career investigation was highest. In addition, among the sub-areas of nursing professionalism, the specialty of nursing was highest. 2. Major satisfaction was higher in those from the Department of Natural Science, with a nurse in their family members and it was also significantly different according to the motive for choosing nursing. Nursing professionalism was higher in those with a nurse in their family members and it was also significantly different according to the motive for choosing. 3. A statistically significant positive correlation was observed between the subjects' major satisfaction and their nursing professionalism. The sub-area of major satisfaction in the highest correlation with nursing professionalism was 'satisfaction with perception', and the sub-area of nursing professionalism in the highest correlation with major satisfaction was 'professional self-concept' CONCLUSION : A significant positive correlation was observed between major satisfaction and nursing professionalism, and this raises the necessity for maintaining high major satisfaction so that nursing students may establish positive nursing professionalism through their college study.

만성신부전 환자에서 심근 troponin I와 T 및 CK-MB의 진단적 의의

윤정이(Jung E Yun),이형철(Hyung Cheol Lee),이준상(June Sang Lee),박규용(Kyu Yong Park),박대균(Dae Kyun Park),구자룡(Ja Ryong Goo),김형직(Hyung Jik Kim),김근호(Gheun Ho Kim),전노원(Rho Won Chun),채동완(Dong Wan Chae),노정우(Jung Woo N 대한내과학회 2000 대한내과학회지 Vol.58 No.2

N/A Background : Nonspecific elevations of CK-MB, cTnT have been well known in patients with chronic renal failure(CRF) on maintenance hemodialysis. It has been suggested that recently developed cTnI seldom shows nonspecific elevations in these patients. Status of CRF patients can be divided into three groups: predialysis group, hemodialysis group and peritoneal dialysis group. Until now, most researchers have studied CK-MB, cTnT and cTnI only in CRF patients receiving maintenance hemodialysis. No previous studies have ever compared the differences of the nonspecific positivity of CK-MB, cTnT and cTnI according to the different status of CRF patients. Methods : Nonspecific positive ratios of cTnI, cTnT, & CK-MB in were evaluated 20 predialysis patients, 13 CAPD patients and 20 hemodialysis patients. No one had had any evidence of myocardial ischemia during the previous 3 months before the study entry. The predialysis group was again divided into two groups according to the cut off level of serum creatinine of 3.0 mg/dl. Authors also compared the nonspecific positive ratios of cTnI, cTnT, CK-MB between diabetic CRF group and non diabetic CRF group. The sensitivity, specificity and false positive ratios of each enzymes were examined on and 6 hours after arrival in 21 CRF patients who visited the emergency room with the complaint of chest pain. Results : 1) There were no nonspecific significant elevations of cTnI in CRF patients regardless of the status of CRF. But there were significant nonspecific elevations of CK-MB, cTnT in them. It was more marked in cTnT especially with the cut-off value of 0.1 ng/ml. 2) Nonspecific positive ratios of cTnT was significantly increased in diabetic CRF patients. 3) The sensitivity and specificity of cTnI were 100% and 93.3% each, which were significantly higher than those of CK-MB(83.3%, 66.7%) & cTnT(66.7%, 53.3%). Conclusion : In CRF patients, the nonspecific positive ratios of CK-MB, cTnT were higher than that of cTnI, and only cTnI did show significant specific elevations in all the CRF patients with acute myocardial infarction. It is likely that the status of CRF patients, dialysis mode, the sampling time point would not give significant changes in the nonspecific positive ratios of CK-MB, cTnT and cTnI.(Korean J Med 58:204-212, 2000)

Reduced-Intensity Conditioning with Busulfan, Fludarabine, and Antithymocyte Globulin for Hematopoietic Cell Transplantation from Unrelated or Haploidentical Family Donors in Patients with Acute Myeloid Leukemia in Remission

Lee, K.H.,Lee, J.H.,Lee, J.H.,Kim, D.Y.,Park, H.S.,Choi, E.J.,Ko, S.H.,Seol, M.,Lee, Y.S.,Kang, Y.A.,Jeon, M.,Baek, S.,Kang, Y.L.,Kim, S.H.,Yun, S.C.,Kim, H.,Jo, J.C.,Choi, Y.,Joo, Y.D.,Lim, S.N. Kluge Carden Jennings Pub. Co 2017 BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION Vol.23 No.9

To investigate the role of antithymocyte globulin (ATG)-containing reduced-intensity conditioning (RIC) in hematopoietic cell transplantation (HCT) from unrelated (UD) or haploidentical family donors (HFD), we conducted a phase 2 trial of 237 patients (age range, 16 to 69 years) with acute myeloid leukemia (AML) in remission. Patients undergoing UD-HCT (n@?=@?93) or HFD-HCT (n@?=@?59) received RIC comprising busulfan, fludarabine, and ATG, 9@?mg/kg, whereas those undergoing HCT from matched sibling donors (MSD, n@?=@?85) received myeloablative busulfan and cyclophosphamide conditioning or aforementioned RIC with ATG, 4.5@?mg/kg. For graft-versus-host disease (GVHD) prophylaxis, cyclosporine and methotrexate were administered. The median follow-up period was 44.7 months after HCT for 161 survivors. For UD-HCT versus HFD-HCT, there were no significant differences in leukemia recurrence, nonrelapse mortality, relapse-free survival, grades 2 to 4 acute GVHD, and moderate-to-severe chronic GVHD. Furthermore, when the outcomes of UD-HCT and HFD-HCT were combined and compared with those of MSD-HCT, there were no significant differences in leukemia recurrence (3-year cumulative incidence, 30% versus 29%), nonrelapse mortality (3-year cumulative incidence, 7% versus 8%), relapse-free survival (3-year estimate, 63% versus 63%), and grades 2 to 4 acute GVHD (120-day cumulative incidence, 16% versus 13%). Moderate-to-severe chronic GVHD, however, occurred less frequently in UD/HFD-HCT (2-year cumulative incidence, 22% versus 40%; P@?=@?.006). The addition of ATG to conditioning regimen was a significant predictor for less chronic GVHD (subdistribution hazard ratio, .59). In AML in remission, UD/HFD-HCT after ATG-containing RIC achieved leukemia control equivalent to that of MSD-HCT. Despite HLA disparity in UD/HFD-HCT, chronic GVHD occurred less frequently after ATG-containing RIC, suggesting a strong GVHD-modulating effect of ATG.

Transplantation of Neural-Induced Mesenchymal Stem Cells for Spinal Cord Injury in Dogs

E. Y. Kim,J. H. Lee,K. J. Kim,K. S. Park,Y. B. Park,Y. N. Ha,M. Kim,K. W. Han,X. X Li,J. Yu,D. S. Lee,J. Y. Park,M. K. Kim 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1

Cell transplantation therapy using adult stem cells has recently been identified as a potential treatment for spinal cord injury (SCI). But, recovery after traumatic SCI is very limited. As dogs are physiologically much more similar to human compared with other traditional mammalian models in disease presentation and clinical responses, a number of researches demonstrated canis familiaris is a suitable model for human diseases. This study investigated the effect of transplantation of canine Mesenchymal Stem Cells (cMSC) and neural-induced cMSC (nMSC) to understand how these cells improve neurological function in canine SCI model. The differentiation of cMSC into neural precursor cells was induced in dulbecco’s modified eagle’s medium supplemented with N2-supplement, dibutyryl cyclic adenosine monophosphate, and butylated hydroxyanisole. SCI was induced between T1 and T2 by surgical hemi-section in adult dogs, and then assigned to two groups according to the applied cell types (cMSC vs nMSC). Pelleted cMSC or nMSC were transplanted directly into the injured site after SCI, respectively. Analysis of motor function after transplantation was evaluated by modified Olby score. Magnetic resonance imaging (MRI), histological and immunohistichemical analysis were also performed. Functional recovery in group of cMSC was increasing gradually after transplantation and was higher than nMSC. In MRI, we could not confirm any difference between the cMSC and nMSC experimental groups. Immunohistochemically, beta3-tubuline and nestin were observed in injury site of two experimental groups with the expression level close to non-injured groups. Transplantation of mesenchymal stem cells could promote neuronal reconstruction and repair motor function in SCI. These showed mesenchymal stem cells could be a great candidate as a therapeutic tools in degeneration disease, and dogs could be used to explore human regenerative medicine as a promising animal model. This research was supported by iPET (Grants 110056032CG000), Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea.

A numerical study on the mechanical characteristics of zinc oxide-based transparent thin film transistors.

Lee, D-K,Park, K,Ahn, J-H,Lee, N-E,Kim, Y-J American Scientific Publishers 2011 Journal of Nanoscience and Nanotechnology Vol.11 No.7

<P>Zinc Oxide (ZnO) based Thin Film Transistors (TFTs) have been fabricated and analyzed to investigate mechanical characteristics regarding the stress, strain and deformation of electro circuits using the Finite Element Method (FEM). As the best compromise between the stretching and bending abilities, the coating thickness of SU-8 can be as important for bendability as a neutral mechanical plane. The neutral mechanical plane in electro circuits was designed for obtaining flexibility, e.g., bendability, in a previous numerical study. After that, through experimental validation, we observed what degree of SU-8 thickness was attributable for improved mechanical stability. The results suggest that not only numerical but also experimental measurements of the deformation and SU-8 coating thickness in electro circuits are useful for enhancing structural stability.</P>

A prebiotic effect of Ecklonia cava on the growth and mortality of olive flounder infected with pathogenic bacteria

Lee, W.,Ahn, G.,Oh, J.Y.,Kim, S.M.,Kang, N.,Kim, E.A.,Kim, K.N.,Jeong, J.B.,Jeon, Y.J. Academic Press 2016 FISH AND SHELLFISH IMMUNOLOGY Vol.51 No.-

<P>Olive flounder (Paralichthys olivaceus), also known as the Japanese flounder in Japan, is one of the most important commercial marine finfish species cultured in Korea and Japan. The purpose of this study was to evaluate how a species of brown algae (Ecklonia cava, E. cava) affects the growth rate of olive flounder and its immune response to pathogenic bacteria. First, the experimental fish were divided into four groups: the control group was fed the diet containing only 1.0% Lactobacillus plantarum (L. plantarum), group I was fed 1.0% L. plantarum and 1.0% E. cava (EC), group II was fed 1.0% L plantarum and 0.1% ethanol extract of EC (EE), and group III was fed 1.0% L plantarum and 0.5% EE. The diets fed to the fish twice a day for 16 weeks. The results indicated that supplementation with 1.0% EC and 0.1% EE improved the growth and body weight of olive flounder, and decreased its mortality. This diet, however, did not significantly affect the biochemical profiles of the experimental flounder. The supplementation of 1.0% EC also enhanced the innate immune response of the fish, as evidenced by the high respiratory burst, and increased serum lysozyme and myeloperoxidase activity. The addition of 1.0% EC and either 0.1% or 0.5% EE also decreased the accumulative mortality of olive flounder infected by pathogenic bacteria (Edwardsiella tarda, Streptococcus iniae, and Vibrio harveyi). Overall, these results suggest that E. cava can act as a prebiotic by improving the innate immune response in fish infected with pathogenic bacteria as increased the growth of the probiotic. (C) 2016 Elsevier Ltd. All rights reserved.</P>

How do extreme wet events affect rice quality in a changing climate?

Lee, M.S.,Kang, B.M.,Lee, J.E.,Choi, W.J.,Ko, J.,Choi, J.E.,An, K.N.,Kwon, O.D.,Park, H.G.,Shin, H.R.,Lee, I.,Kim, J.K.,Kim, H.Y. Elsevier 2013 Agriculture, ecosystems & environment Vol.171 No.-

Despite the growing body of evidence that ongoing warming modifies the cereal quality of several crop species, little is known about how it interacts with other climate change drivers. Here, we examined how elevated [CO<SUB>2</SUB>], warming, and extreme wet events (EWEs) would affect the grain quality of a temperate rice (Oryza sativa L.). In 2009 and 2010, plants were grown at two levels of [CO<SUB>2</SUB>] (c. 390 and 650μll<SUP>-1</SUP>) and three levels of warming (ambient, +1.2<SUP>o</SUP>C and +2.2/2.4<SUP>o</SUP>C) over the seasons in six independent temperature gradient field chambers (three each for ambient and elevated [CO<SUB>2</SUB>]). From 20 days after heading, plants were exposed to EWE with a simulated rainfall of 30mm a day and cloud cover by shading (c. 18% of full sunlight) for 10 consecutive days. There were also control plots, which were non-treated (NT) in terms of EWE. We found that warming and EWE, both independently and in their interaction, lead to a significant reduction in the percentages of sound grains, whereas elevated [CO<SUB>2</SUB>] does not seem to do so. Not only warming and EWE, but also elevated [CO<SUB>2</SUB>] resulted in a significant increase of immature chalky grains (ICGs), which mostly consisted of milky-white chalky grains, degrading the grain quality. In addition, warming interacted positively with elevated [CO<SUB>2</SUB>] on ICGs, whereas the warming sensitivity of IGCs was moderated in EWEs: e.g., on average, the % of ICGs in ambient vs. elevated [CO<SUB>2</SUB>] were 8.5% vs. 9.4%, 10.7% vs. 18.1% and 17.8% vs. 29.8% at ambient (24.7<SUP>o</SUP>C), +1.2<SUP>o</SUP>C and +2.4<SUP>o</SUP>C, respectively, whereas they in NT vs. EWE were 1.2% vs. 16.6%, 7.7% vs. 21.1% and 19.4% vs. 28.2%. Our results tightly confirmed that elevated [CO<SUB>2</SUB>] leads to a significant reduction in rice grain protein content (PC). While warming had little effect on the PC, EWE had a significant positive effect, and the EWE-induced increase in PC was smaller in elevated (+3.3%) than in ambient [CO<SUB>2</SUB>] (+10.9%). Given the rice quality predictor of ICGs and PC, our findings imply that expected climate change and weather extremes have a great potential to degrade the quality of rice, in terms of its milling, cooking, eating, and nutritional quality, as well as market value.