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고관영,명노훈,하종성,이창수,최창권,최병용,허기영,김광수,김동수,장종훈,장윤철,김세환,오정민,하민정,박향자 울산과학대학 2000 연구논문집 Vol.27 No.1
본 연구는 자체 제작한 전체 패드가 부착된 드로잉 금형을 이용하여 SUS, 연강, AI, 6:4황동 판재를 드로잉 가공하여 생산된 제품의 특성을 자유드로잉 하여 얻은 제품의 특성과 비교 분석하였다. SUS 판재의 경우 제품에 발생하는 현상은 벽 주름, 구변주름, 수직 파단, 드로잉상처, 이어링이 발생하였으며 길이변화는 펀치어깨부분에서 가장 많이 늘어남을 볼 수 있었고 벽 주름 부분에 있어서 접혀진 부분은 상대적으로 적게 늘어남을 볼 수 있었다. 연강 판재는 SUS 판재에서 보여준 현상을 나타내었으나 SUS 판재에 비해 수직 파단은 적게 일어난 반면 이어링이 더 크게 일어났다. AI 판재의 경우 수직 파단은 일어나지 않았으나 플랜지 주름과 밑면터짐이 발생하였다. 6:4황동의 경우는 전체패드의 AI 판재 드로잉에서와 같이 밑면터짐과 플랜지 주름의 발생이 일어났다.
패스틴^� 첨가가 단백질 분해율과 반추위 발효 및 영양소 소화율에 미치는 영향
최유지,최낙진,박성호,송재용,엄재상,고종렬,하종규 한국동물자원과학회 2002 한국축산학회지 Vol.44 No.5
본 시험은 패스틴^R을 첨가하였을 때, in vitro 상에서 단백질 fraction과 분해율에 미치는 영향과, in vivo 상에서 반추위 성상, 미생물 군집, 암모니아태 질소 농도 및 영양소 소화율에 미치는 영향을 규명하고자 실시하였다. In vitro 실험에서는 1㎜로 분쇄된 대두박을 기질로 하여 패스틴^R(㈜은진인터내셔날)을 첨가하여 borate-phosphate buffer와 중성세제에서의 조단백질 분해율을 측정하였으며, exogenous enzyme (Streptomyces griseus 유래 protease)를 이용하여 39℃에서 0, 2, 4, 8, 12, 48 시간동안 배양 후 조단백질 분해율을 측정하였다. 반추위 발효성상과 영양소 소화율은 반추위 fistula가 부착된 평균체중 300㎏의 홀스타인 수소 4두를 이용하여 무첨가구, 패스틴^R 첨가구의 두 개 처리구에 2마리씩 4마리를 배치하여 측정하였다. Buffer Soluble Protein fraction은 패스틴^R 첨가 수준별로 차이가 없었으나, 무첨가구에 비해 패스틴^R 첨가구에서 감소하는 경향을 보였다. 단백질 분해율은 배양 0 시간대에서 4시간대까지는 처리구간 유의성이 없었지만, 12 h과 48 h에서는 패스틴^R 첨가로 시험구에서 감소되었다. 용해 단백질 분해율 “a”는 패스틴^R 시험구에서 경미하게 높은 수치를 나타내었지만, 소화 가능한 단백질 분해율 “a+b”는 패스틴^R 시험구에서 낮은 경향을 보였다. 패스틴^R 첨가로 pH와 NH_3-N 농도는 증가하는 경향이었으며 휘발성지방산, 미생물 수 및 enzyme activity는 감소하였고 영양소 소화율은 높았으나 유의적인 차이는 없었다. This study, including two in vitro experiments and an invivo experiment were conducted to evaluate effects of Passtein^R on crude protein degradability, ruminal fermentation characteristics and nutrient digestibility. In in vitro experiment protein degradability was examined using borate-phosphate buffer and neutral detergent, and using protease from Stroptomyces griseus at 39℃ for 0, 2, 4, 8, 12 and 48 h. In addition, an in vivo experiment was conducted in a switch back design and ruminal fermentation and nutrient digestibility were determined. Four ruminal-fistulated Holstein cows weighing 300㎏ in mean body weight randomly allotted to 2 treatments (control and Passtein^R supplementation). Although there was no significant difference on protein fraction between treatments, it appears that Passtein^R supplementation decreased buffer soluble protein fraction compared to control. Protein degradability was not affected by Passtein^R from 0 h to 4 h, but decreased at 12 h and 48 h compared to control. Degradation of immediately degradable fraction was higher in Passtein^R treatment, but degradation of fermentable fraction was lower in Passtein^R treatment compared to control. The pH and NH_3-N concentration tended to increase in Passtein^R treatment, but VFA production, microbial counts and enzyme activity tended to decrease in Passtein^R treatment compared to control. In addition, nutrient digestibility in the total tract tended to increase in Passtein^R treatment compared to control.
Transplantation of Neural-Induced Mesenchymal Stem Cells for Spinal Cord Injury in Dogs
E. Y. Kim,J. H. Lee,K. J. Kim,K. S. Park,Y. B. Park,Y. N. Ha,M. Kim,K. W. Han,X. X Li,J. Yu,D. S. Lee,J. Y. Park,M. K. Kim 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1
Cell transplantation therapy using adult stem cells has recently been identified as a potential treatment for spinal cord injury (SCI). But, recovery after traumatic SCI is very limited. As dogs are physiologically much more similar to human compared with other traditional mammalian models in disease presentation and clinical responses, a number of researches demonstrated canis familiaris is a suitable model for human diseases. This study investigated the effect of transplantation of canine Mesenchymal Stem Cells (cMSC) and neural-induced cMSC (nMSC) to understand how these cells improve neurological function in canine SCI model. The differentiation of cMSC into neural precursor cells was induced in dulbecco’s modified eagle’s medium supplemented with N2-supplement, dibutyryl cyclic adenosine monophosphate, and butylated hydroxyanisole. SCI was induced between T1 and T2 by surgical hemi-section in adult dogs, and then assigned to two groups according to the applied cell types (cMSC vs nMSC). Pelleted cMSC or nMSC were transplanted directly into the injured site after SCI, respectively. Analysis of motor function after transplantation was evaluated by modified Olby score. Magnetic resonance imaging (MRI), histological and immunohistichemical analysis were also performed. Functional recovery in group of cMSC was increasing gradually after transplantation and was higher than nMSC. In MRI, we could not confirm any difference between the cMSC and nMSC experimental groups. Immunohistochemically, beta3-tubuline and nestin were observed in injury site of two experimental groups with the expression level close to non-injured groups. Transplantation of mesenchymal stem cells could promote neuronal reconstruction and repair motor function in SCI. These showed mesenchymal stem cells could be a great candidate as a therapeutic tools in degeneration disease, and dogs could be used to explore human regenerative medicine as a promising animal model. This research was supported by iPET (Grants 110056032CG000), Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea.
Tunable threshold voltage in solution-processed single-walled carbon nanotube thin-film transistors
Seong, N.,Kim, T.,Kim, H.,Ha, T.J.,Hong, Y. Elsevier 2015 CURRENT APPLIED PHYSICS Vol.15 No.suppl1
We have investigated tunable threshold voltage in solution-processed single-walled carbon nanotube thin-film transistors (SWCNT TFTs) employing a simple and reproducible method of chemical encapsulation. Compared to a pristine one, SWCNT TFTs encapsulated with ammonium hydroxide (NH<SUB>4</SUB>OH) and nitric acid (HNO<SUB>3</SUB>) exhibit a negative shift and a positive shift in threshold voltage, respectively. Such results can be explained by the modification of the energy band at the interface between the source metal electrode and the SWCNT network. By using the Y-function method, we also characterized electrical properties such as field-effect mobility, threshold voltage, and contact resistance for TFTs treated with NH<SUB>4</SUB>OH or HNO<SUB>3</SUB>. The technique to favorably tune threshold voltage in solution-processed SWCNTs is significant for constituting CNT-based nanoelectronics.