동종 골수 이식한 백혈병에서 단반복 유전자로 살펴본 혼합 키메라 현상의 의의

장대영,이정신,서철원,이규형,이제환,지현숙,박찬정,한면수,최동원,김정균,최성준,김성배,김상위,김우건,김상희 대한조혈모세포이식학회 1998 대한조혈모세포이식학회지 Vol.3 No.1

연구배경 : 동종 골수 이식한 백혈병에서 키메라 현상의 분석은 착상, 질병 재발과 이식 거부를 이해하는데 도움을 줄 수 있다. 최근 STR을 PCR로 증폭한 검사법에 의해 매우 민감하고 유용하게 혼합 키메라 현상을 검출할 수 있다고 보고되고 있으나, 이 방법의 민감도와 임상적 의의는 아직 완전히 정립되지는 않았다. 따라서 PCR-STR검사법이 혼합 키메라 현상을 민감하게 검출하는지 보고, 동종 골수 이식 후 혼합 키메라 현상의 변화 양상을 관찰하고, 키메라 현상의 임상적 의의를 보고자 하였다. 방법 : 형제로부터 동종 골수 이식한 급성 백혈병 6명과 만성 백혈병 2명의 모두 8명을 대상으로 하였다. 44개 골수 검체에서 phenol/chloroform방법에 의하여 단핵세포 DNA를 추출하였다. DNA는 CTT 삼중체, vWA와 amelogenin시발체를 이용하여 증폭하였다. 증폭 산물은 5% 폴리아크랄아미드 젤에서 전기영동 뒤 은 염색으로 확인하였다. 2명의 DNA를 여러 비율로 혼합하여 상기 검사를 하여 검출 가능 최소 농도를 결정하였다. 결과 : 상기 검사법의 검출 가능 최소농도는 0.25%였다. 혼합 키메라 현상은 8명중 4명에서 관찰되었다. 혼합 키메라 현상은 보인 1명에서 급성 및 만성 이식편대 숙주 질환이 나타났고, 완전 공여자 키메라 현상을 보인 1명에서 만성 이식편 대 숙주 질환이 나타났다. 점진적으로 증가하는 혼합 키메라 현상을 보인 2명은 이식 후 12개월에 질병 재발을 보였으나, 완전 공여자 키메라 현상을 보인 경우엔 재발이 관찰되지 않았다. 결론 : PCR-STR검사법은 동종 골수 이식 후 남아 있는 환자 세포를 검출하고 키메라 현상을 관찰하는데 유용한 방법이다. 또한 이 검사법은 동종 골수 이식 후 재발의 조기 진단, 이식편 대 숙주 질환의 평가와 면역 관용에 임상적으로 응용할 수 있으리라 생각한다. Background : Chimerism analysis after allogeneic bone marrow transplantation (BMT) for leukemia could be helpful understanding of the early marrow engraftment, disease relapse, and graft rejection. Recently, a PCR technique which amplifies short tandem repeats(STR) has been reported to be highly sensitive and reliable in detecting mixed chimerism. But its sensitivity and clinical significance has not been established. The purpose of this study was firstly, to confirm whether a PCR-STR in highly sensitive enough ti assess mixed chimerism, secondly to monitor the changing patterns of mixed chimerism after allogeneic BMT, and lastly to determine correlation between the chimeric status and the clinical outcome. Methods : A study was made eight patients (six with acute leukemia and two with chronic leukemia) who underwent unmanipulated allogeneic BMT along with matched donors. DNA was extracted from mononuclear cells of 44 bone marrow samples using a phenol/chloroform method. Amplification of DNA was done using CSFIPO-TPOX-TH01(CTT) triplex with or without vWA or amelogenin primer. The amplified product was separated on 5% polyacrylamide gel and was confirmed by silver staining. The sensitivity of CTT triplex method was determined by mixing DNA from two person in serial proportion using the same method. Results : The sensitivity for CTT triplex method was 0.25% Mixed chimerism was documented in 4 of 8 patients. ONe of four patients with mixed chimerism developed acute and chronic graft versus host disease (GVHD) and one of four patients with complete donor chimerism also developed chronic GVHD. Two patient with progressive mixed chimerism relapsed at 12 months post-BMT, whereas no patient with complete donor chimerism has relapsed. Conclusion : The study has found that PCR-STR was an effective method for the detecting residual host cell and monitoring the chimeric status after allogenic BMT. PCR-STR can also be clinically applicable in early prediction of relapse, appropriate assessment of GVHD, as well as tolerance after allogeneic BMT.

재무역량 분석을 통한 국내 통신산업 수익원 변화 연구

김남면,이정기,배기수 한국세무회계학회 2003 세무회계연구 Vol.13 No.-

본 논문의 목적은 정보통신기술의 눈부신 발전에 따른 통신사업에서의 수익원의 변화를 재무제표 분석을 통해 살펴봄으로서 객관성과 신뢰성을 확보하여 정보이용자에게 유용한 정보를 제공하는데 있다. 연구 결과 KT를 제외한 타 유선사업자의 건전성은 이통사업자의 건전성보다 저조한 것으로 분석되었으며, KT, SK텔레콤, LG텔레콤의 유동성은 점차 좋아진 반면, 하나로통신의 유동성은 악화도고 있는 것으로 분석되었다. 또한 두루넷의 경우는 호전은 되고 있었지만 타 사업자보다 월등히 저조하게 분석되었다. 수익성 역시 SK텔레콤이 가장 양호한 것으로 분석되었으며, 두루넷·하나로통신이 가장 낮은 것으로 분석되었다. 성장성은 이통사업자와 두루넷이 빠른 속도로 성장하고 있는 것으로 분석되었으며, 활동성은 유선사업자에서는 두루넷, 이통사업자에서는 SK텔레콤의 활동성이 활발한 것으로 분석되었다. 이상과 같은 분석결과를 토대로 유선통신서비스에서 무선통신서비스로의 수익원이 변하고 있음을 알 수가 있다. 급변하는 통신시장에서 수익원의 변화를 신뢰성있는 재무자료를 이용하여 분석한 본 연구결과는 통신사업자 및 정보 이용자들의 경제적 의사결정시 객관적인 자료로 유용하게 이용될 수 있다.

만성환자의 재활간호계획을 위한 조사연구

최은옥,정면숙 최신의학사 1989 最新醫學 Vol.32 No.1

男子大學生 內衣의 購買 行動에 關한 硏究

朴敏予,鄭炫珠 慶熙大學校 1993 論文集 Vol.22 No.-

For the present study we developed questionaire which contains 35 questions. Starting from the previously tested ones we examined them, consulted with releated industries on the contents and tested before finalizing them. Twice all toghther, once in July and the other in September of 1993, the questionaire was performed to the male students of the eight universities located in Seoul and Daeduk. The number collected was 350 but 290 among them were usable. For the statistical analysis of the results the SPSS computer package was used and the main characteristic results are in terms of frequency, percentile, t-test and χ²- analysis. The followings are the main results. 1. Purchasing was done without planning, whenever needed, at the first shop of visit and within a half an hour. 2. No pattern or geometric one were the most favorite patterns on the underwear and white color was overall favorite but for science and engineering major students preferred color was the red color. 3. Likely to purchase repeatedly at the same shop and the reason is convenient location. Trade marks were not the criterion for the purchase and the trade marks of the domestic three major companies were well known while ones from other domestic companies were mistaken as the foreign ones. 4. The relationship between the advertisement and actual purchasing tend to have some time lags and it may depend on, among other reasons, how long the merchandise has been on the market. 5. As to the favorite locations of the purchase, the manufacture´s exclusive store is most favored one and reasons given for shop in the market is the cheap price, and the department store the convenience in transportation together with the variety of items and choices. And those who do not care about the trade mark tend to wear longer the underwear longer. In fact they even do not recall how long they actually wear those. 6. For foreign products, most do not have any experiences but for those who actually purchased them they believed the higher priced merchandise were better quality commodities and also the comfortableness and curiosity were reasons for the frequent pruchasing behavior.

랫드에서 미생탕의 4주 반복투여독성

김태명, 류재면, 서임권, 박동선, 조정희, 장용훈, 김윤배, 황석연 충북대학교 동물의학연구소 2009 Journal of Biomedical and Translational Research Vol.10 No.1

본문참고

한국에서의 간호중재분류체계(NIC)구조 타당성 검증

박성애,박정호,정면숙,박성희,염영희 서울대학교 간호대학 간호과학연구소 1999 간호학 논문집 Vol.13 No.2

The purpose of this study was to validate the NIC taxonomy in Korea. A sample of 10 nurse experts who were head nurses at a university affiliated hospital and had some knowledge and experience with research method and NIC rated, the NIC taxonomy twice using five criteria: clarity; homogeneity; inclusiveness; mutual exclusiveness; theory neutral. The NIC Taxonomy Evaluation questionnaire developed by the Iowa Intervention project team was used for data collection. The instrument was translation and back-translation. The results were as follows: 1. About fifty-eight percent of the respondents rated the domains as either quite characteristic or very characteristic on all criteria and about seventy -one percent of the respondents rated the classes as either quite characteristic or very characteristic on all criteria. 2. The criterion of theory neutral received the highest ratings and the criteria of clarity and inclusiveness received the lowest ratings on domains. 3. The criterion of theory neutral received the highest ratings and mutual exclusiveness received the lowest ratings on classes. 4. The Physiological: Complex domain received the highest ratings and the Family domain received the lowest ratings. These findings suggest that Nursing Interventions Classification System sensitive to Korean culture need to be developed.

동일한 시료에 대한 국내 기관간의 STR 분석결과 비교 : STR 유전자좌 분석법의 표준화 설정을 위하여

박종태,신경진,양윤석,우광만,이숭덕,이승환,이정빈,정연보,조승희,한길로,한면수,홍승범 大韓法醫學會 2001 대한법의학회지 Vol.25 No.1

This paper described a collaborative exercise intended to see what kinds of short tandem repeat (STR) loci are used in different DNA typing laboratories in Korea and to compare their results for the demonstration whether uniformity of DNA profiling results from different laboratory could be achieved in Korea Laboratories were asked to test five tissue DNAs using methods routinely used in each laboratory and to report the results to the coordinating laboratory. The exercise demonstrated that each laboratory was using different STR loci for the typing with different STR numbers,2 VNTRS,36 STRs and amelogenin in total, and the direct comparison of the results from all the laboratory for the 18 loci could not be done as only one laboratory submitted typing results. Among 21 loci for which several laboratories submitted typing results, results for 14 loci were the same and results for the other 7 loci were different depending on the participating laboratory. D1S80, F13A01, D16S539, D21S11, D18S51, D3S1744 were the loci with different typing results. Even in the cases where commercial kits were used, the results were not the same depending on the machines used, that is the capillary electrophoresis or the gel based electrophoresis. The reason for the different results, points about the standardization of the methods arid the profiling data were described.

Noninvasive diagnosis and evaluation of curative surgery for gastric cancer by using NMR-based metabolomic profiling.

Jung, Jeeyoun,Jung, Youngae,Bang, Eun Jung,Cho, Sung-il,Jang, You-Jin,Kwak, Jung-Myun,Ryu, Do Hyun,Park, Sungsoo,Hwang, Geum-Sook Raven Press 2014 Annals of Surgical Oncology Vol.21 No.suppl4

<P>Mass screening for gastric cancer (GC), particularly using endoscopy, may not be the most practical approach as a result of its high cost, lack of acceptance, and poor availability. Thus, novel markers that can be used in cost-effective diagnosis and noninvasive screening for GC are needed.</P>

Colorectal cancer

Jung Myun Kwak 대한외과학회 2015 대한외과학회 학술대회 초록집 Vol.2015 No.11

Regional Difference in Colonic Motility Response to Electrical Field Stimulation in Guinea Pig

( Jung Myun Kwak ),( Reji Babygirija ),( Irena Gribovskaja Rupp ),( Toku Takahashi ),( Shigeru Yamato ),( Kirk Ludwig ) 대한소화기기능성질환·운동학회 2013 Journal of Neurogastroenterology and Motility (JNM Vol.19 No.2

Background/Aims In isolated guinea-pig colon, we investigated regional differences in peristalsis evoked by intrinsic electrical nerve stimulation. Methods Four colonic segments from mid and distal colon of Hartley guinea pigs, were mounted horizontally in an organ bath. Measurement of pellet propulsion time, intraluminal pressure, electrical field stimulation (EFS; 0.5 ms, 60 V, 10 Hz), and response of pharmacological antagonists, were performed to isolated segments of colon to determine the mechanisms underlying peristaltic reflexes evoked by focal electrical nerve stimuli. Results In fecal pellet propulsion study, the velocity of pellet propulsion was significantly faster in the distal colon and decreased gradually to the proximal part of the mid colon. Intraluminal pressure recording studies showed that luminal infusion initiated normal peristaltic contractions (PCs) in 82% trials of the distal colon, compared to that of mid colon. In response to EFS, the incidence of PCs was significantly increased in the distal colon in contrast, the incidence of non-peristaltic contractions (NPCs) was significantly higher in the middle-mid colon, distal-mid colon and distal colon, compared to that of proximal-mid colon. Addition of L-NAME into the bath increased the frequency of NPCs. EFS failed to cause any PCs or NPCs contractions in the presence of hexamethonium, atropine or tetrodotoxin. Conclusions This study has revealed that electrical nerve stimulation of distal colon is the most likely region to elicit a peristaltic wave, com - pared with the mid or proximal colon. Our findings suggest that EFS-evoked PCs can be modulated by endogenous nitric oxide.