Genetic and Phenotypic Characterization of Rice Backcrossed Inbred Sister Lines of Saltol in Temperate Saline Reclaimed Area

( Jae-hyuk Han ),( Na-hyun Shin ),( Je-hoon Moon ),( Changhwan Yi ),( Soo-cheul Yoo ),( Joong Hyoun Chin ) 한국육종학회 2020 Plant Breeding and Biotechnology Vol.8 No.1

Saltol is one of the most well-known quantitative loci (QTLs) for salinity tolerance in rice. It has been used to develop highly tolerant rice varieties in saline and coastal areas in Southeast Asia, South Asia, and Africa. However, the functional activity of Saltol is not well known, and the molecular marker application of readily developed linked markers in Saltol has not always been successful in the rice breeding programs for salinity tolerance improvement. Interestingly, two BC₂F₉ sister backcrossed inbred lines (BILs), which have been developed by marker-assisted backcrossing utilized the linked markers of Saltol to improve the salinity tolerance of MS11 (a temperate japonica growing in tropical condition). The BILs showed very different phenotypic and stress tolerance, although both contained the Saltol QTL. The genomic similarity of the two BILs was 73%, and we have identified the genomic sites of different genic constitutions between the lines utilizing background genotyping. The stress response of the two BILs showed difference in survival rate, grain yield under highly saline field condition, and SPAD, SES in hydroponic conditions. MS11-SaltolA showed salinity tolerance through Na⁺/K⁺ homeostasis with relatively high K⁺ ion uptake and low Na⁺ ion uptake in the seedling stage. Further genomic analyses with whole genome resequencing is ongoing to study on gene interactions. The developed highly tolerant MS11-SaltolA can be used as an improved donor in rice molecular breeding for high salinity tolerance.

Canine susceptibility to human influenza viruses (A/pdm 09H1N1, A/H3N2 and B)

Song, Daesub,Kim, Hyekwon,Na, Woonsung,Hong, Minki,Park, Seong-Jun,Moon, Hyoungjoon,Kang, Bokyu,Lyoo, Kwang-Soo,Yeom, Minjoo,Jeong, Dae Gwin,An, Dong-Jun,Kim, Jeong-Ki Society for General Microbiology 2015 The Journal of general virology Vol.96 No.2

<P>We investigated the infectivity and transmissibility of the human seasonal H3N2, pandemic (pdm) H1N1 (2009) and B influenza viruses in dogs. Dogs inoculated with human seasonal H3N2 and pdm H1N1 influenza viruses exhibited nasal shedding and were seroconverted against the viruses; this did not occur in the influenza B virus-inoculated dogs. Transmission of human H3N2 virus between dogs was demonstrated by observing nasal shedding and seroconversion in naïve dogs after contact with inoculated dogs. The seroprevalence study offered evidence of human H3N2 infection occurring in dogs since 2008. Furthermore, serological evidence of pdm H1N1 influenza virus infection alone and in combination with canine H3N2 virus was found in the serum samples collected from field dogs during 2010 and 2011. Our results suggest that dogs may be hosts for human seasonal H3N2 and pdm H1N1 influenza viruses.</P>

양식넙치, Paralichthys olivaceus 치어의 스쿠티카충 감염경로

진창남,이창훈,오상필,나오수,허문수 한국어병학회 2003 한국어병학회지 Vol.16 No.1

넙치양식시 피해가 많은 스쿠티카충의 치어기 감염경로를 규명하기 위하여 2001년도에 제주도지역 넙치양식장과 종묘배양장을 대상으로 조사를 실시하였다. 종묘생산 과정에서의 감염경로 조사를 위해 먹이생물인 클로렐라, 알테미아, 로티퍼 배양수조를 조사한 결과 로티퍼 배양수조에서 밑바닥 찌꺼기와 로티퍼 사체 및 생체에서 스쿠티카충 감염이 확인되었다. 스쿠티카충이 감염된 로티퍼를 주어에 투여한 후 자어 사육수조 바닥에는 스쿠티카충이 다량 번식하였다. 넙치 치어에 최초 감염은 부화자어가 착저 완료후 10일경 (부화후 40일경)에 사육수조 바닥에 서식하고 있던 스쿠티카충이 치어에 감염되었다. 스쿠티카충에 감염된 넙치 치어를 조직학적 방법으로 조사하여 감염경로를 확인한 결과, 안구나 뇌에 감염되는 경로는 주둥치 부분이나 등지느러미 앞부분의 표피에 감염된 후 상피조직과 근조직과 신경조직에 따라 뇌로 이행되었다. 그리고 내부장기에 감염되는 경로는 배지느러미나 뒷지느러미 연조막에 침투하여 상피조직과 근조직를 따라 항문과 직장으로 이행되었으며 비뇨생식공을 통해서 요도와 방광, 직장상피세포, 복강, 췌장, 신장외막, 신장 등으로 침투하였다. 스쿠티카충이 감염되지 않은 로티퍼를 이용하는 종묘배양장에서는 먹이생물 배양수조, 자어사육수조, 자어 및 치어 (전장 7㎝ 전후)에서 스쿠티카충이 감염되지 않았다. The infection characteristics with scuticociliates at on-land rearing farms and hatcheries of flounder, Paralithys olivaceus was investigated during they year of 2001 by juvenile infection routes. When culture tanks for living food organisms such as chlorella, rotifer, and Artemia were searched, scuticocilates were detected both in live and dead rotifer, and at the dregs of culture tank bottoms at almost hatcheries. When rotifer infected with scuticocilates fed on fish larvae, lots of scuticocilate were inhabited at the bottom of fry rearing tanks. After feeding on scuticocilates-infected rotifer on fish larvae, first infection was detected at 10 days after bottom dwelling or 40 days old after hatching. By histopathological examination we confirmed the infection route of eyeball of brain contamination was that the ciliate worms digged through mouth and front part of the dosal fin cuticle, transferred into eyeball along the epithelium and muscle tissue, and reached finally into brain by the muscle and nerve tissue. The infection of internal organs was clarified into two routes. The first route was started form the infection at ventral and anal fin rays by the worms, and reached at the anus and rectum through the epithelium and muscle tissue. The second route was initiated from the infection at urinary organ and reached into the rectum epithelium cells, inner wall of intestine, abdominal cavity, pancreas, kidney, and pancreas. At seed production farms where fish larvae fed on scuticocilate-free rotifer, the worms were not detected not only at the food organisms culture tanks and juvenile rearing tanks but also larval flounder less than 7㎝ in total length.

NIHSS 활용을 통한 뇌졸중 환자 간호의 질향상 전략

박경현,송경애,공혜성,나화주,유수경,박소영,김상희,최혜림,배희준,한문구,양미화 한국의료QA학회 2009 한국의료질향상학회지 Vol.15 No.2

문제: 뇌졸중 환자를 돌보는 간호현장에서는 급성기 뇌졸중 환자의 증상악화에 대한 조기사정과 적절한 대처가 매우 중요한 문제이다. 그러나 임상현장에서는 이를 객관화하고 수치화하여 모든 의료진이 의사소통할 수 있는 유용한 도구를 사용하고 있지는 못하는 실정이다. 이러한 문제를 해결하기위해 NIHSS(National Institute of Health Stroke Scale)를 신경과 진료의사를 중심으로활용하고 있지만 아직 간호현장에서는보편적으로사용하지 않고있다. 이에 뇌졸중환자간호에 NIHSS를 활용하여 뇌졸중 증상악화의 조기사정과 빠른 대처로 궁극적으로는 뇌졸중환자 간호의 질을보다 향상시키기 위하여 본활동이 시도되었다. 목적: 첫째, 간호사의 NIHSS 측정 신뢰도의 현수준을 점검하고 이를향상시키기 위한 프로그램을 개발·적용한다. 둘째, 간호사가급성기 뇌졸중환자에게 NIHSS 측정을 통하여 뇌졸중악화를 얼마나 예측할 수 있는지 분석하고 이를 향상시킬수있는 방법을 모색한다. 셋째, 궁극적으로 급성기 뇌졸중 환자의 증상악화의 조기 발견과치료를위한의료진의 올바른 대처지침을 마련하여 이를 적용한다. 의료기관: 경기도에 소재한 대학병원의 뇌졸중 집중치료실 질 향상 활동: 첫째, 간호사의 NIHSS 측정 신뢰도 향상을 위해 「NIHSS 간호사 교육 프로그램」을 마련하였다.

톡소포자충 검출에 있어서 DNA 분리방법 및 반응 조건에 따른 중합효소 연쇄반응의 민감도 비교

이두용,이영하,강문수,나영언,신대환 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.1

To optimize the PCR for the diagnosis of toxoplasmosis, different DNA extraction method, PCR condition to conform the target DNA and Southern blot hybridization were carried out. The results were as follows. 1. On DNA extraction, G NOME DNA isolation kit method from the tachyzoites of Toxoplasma RH strain and split second DNA isolation kit method from the blood of Toxoplasma injected mouse were most effective on yield and purity of DNA products than any other methods. 2. PCR products were relatively dependent upon the variation of MgCl_2 concentration and pH in PCR buffer. 3. As the PCR results after serial dilution, G NOME DNA isolation method from tachyzoites showed the sensitivity to 0.05 fg and the split second DNA isolation kit method showed to 5 pg. In the result, the sensitivity of PCR was increased to 10-1000 times after adding CTAB in these two methods. 4. In two steps PCR, denaturation and annealing, after adding CTAB, G NOME DNA isolation kit method showed sensitivity to 5 pg and split second DNA isolation kit method to 5 ng at the annealing of 72℃ and the former showed the sensitivity to 50 fg and the latter showed the same sensitivity with it at 72℃, at the annealing of 65℃. 5. As the result of comparison to the sensitivity of target DNA with sequntial amplification method and Southern blot hybridization, the one was 100-1000 times more sensitive than that of the other.

[PA-0014] Detection of SNPs variations in the seed storage protein gene involved in β-conglycinin

Soo-Kwon Park(Soo-Kwon Park),Jung-Kyung Moon(Jung-Kyung Moon),Sang-Beom Lee(Sang-Beom Lee),Dool-Yi Kim(Dool-Yi Kim),Mi-Suk Seo(Mi-Suk Seo),Yu-Na Kim(Yu-Na Kim),Gyu-Tae Park(Gyu-Tae Park),June-Yeol Cho 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

Leptin-Evoked Catecholamine Release Through Activation of Protein Kinase and Its Receptors in the Rat Adrenal Medulla

Ki-Soo Kim · Yu-Seok Seo · Gwang-Moon Na · Duck-Mi Na · Hye-Gyeong Shin · Dong-Yoon Lim 조선대학교 의학연구원 2006 The Medical Journal of Chosun University Vol.31 No.1

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수입산 및 국내산 담수어류 3종의 일반성분과 지방산 조성

문수경 ( Soo Kyung Moon ),이두석 ( Doo Seog Lee ),윤호동 ( Ho Dong Yoon ),김연계 ( Yeon Kye Kim ),윤나영 ( Na Young Yoon ),김인수 ( In Soo Kim ),정보영 ( Bo Young Jeong ) 한국수산과학회 2012 한국수산과학회지 Vol.45 No.6

The proximate and fatty acid compositions of the edible portion of three species of cultured freshwater fish (common eel Anguilla japonica, rainbow trout Onchorhynchus mykiss, and Israeli carp Cyprinus carpio) were compared between imports from China and domestically produced specimens. The lipid contents of cultured common eel and rainbow trout were rich in imported f ishes (20.4 and 12.2%, respectively) compared with those in domestic ones (16.0 and 8.01%, respectively), while those of Israeli carp were rich only in the domestically produced specimens (8.06 and 3.07%, respectively). There was a negative correlation between the lipid and moisture contents in all f ish samples (r =-0.86). The protein contents ranged from 16.6 to 21.3% in domestic f ishes and 15.3 to 19.1% in imported ones. The most prominent fatty acids in the f ishes were: saturated fatty acids, 16:0, 18:0 and 14:0; monounsaturated fatty acids, 18:1n-9, 16:1n-7 and 18:1n-7; and polyunsaturated fatty acids (PUFA), 18:2n-6, 22:6n-3 (docosahexaenoic acid, DHA), and 20:5n-3 (eicosapentaenoic acid, EPA). The percentage of n-3 PUFA (e.g., DHA, 22:5n-3, EPA, and 18:3n-3) was higher in domestic common eel and Israeli carp than in imported ones, but similar in domestic and imported rainbow trout, and higher in domestic wild rainbow trout than in cultured ones. On the other hand, all of the cultured freshwater f ishes contained a relatively large amount of 18:2n-6, which is a characteristic fatty acid in cultured f ish lipids.

이온교환체로서 금속-페난드롤린-메페남산 3원 착물을 이용한 메페남산의 정량

허문회(Moon Hye Hur),김대병(Dae Byung Kim),남수자(Soo Ja Nam),문현숙(Hyun Sook Moon),이미나(Mi Na Lee),정문모(Moon Mo Jung),안문규(Moon Kyu Ahn) 대한약학회 2001 약학회지 Vol.45 No.1

A method for the determination of anionic drug, mefenamate with ion-selective electrode using Fe(II)-1,10-phenanthroline chelate as a counter ion was developed. Benzyl nitrophenyl ether (BNPE) plasticized membrane was more selective and sensitive than the other tested membranes. This membrane electrode exhibits a linear response for 10-2 M~5 ×10-5 M of mefenamic acid with a slope of - 61.4mV/dec. in borate buffer sloutions (Ph 9.0). Potentiometric selectivity measurements revealed negligible interferences from various organic and ionorganic anions. Direct potentiometry and potentiometric titration method of mefenamic acid in capsule preparations are presented and compared.

시판 국내산 및 수입산 다슬기(Semisulscospira sp,)의 일반성분 및 지방산 조성

문수경 ( Soo Kyung Moon ),김인수 ( In Soo Kim ),임치원 ( Chi Won Lim ),윤나영 ( Na Young Yoon ),정보영 ( Bo Young Jeong ) 한국수산과학회 2015 한국수산과학회지 Vol.48 No.6

The proximate, fatty acid and dimethyl acetal (DMA) compositions of domestic and imported melania snails Semisulcospira sp. being sold commercially were studied. The protein content ranged from 8.09% (China) to 11.3% (North Korea). The lipid content ranged from 0.84 (China) to 2.36% (North Korea). Domestic melania snail had protein (10.5%) and lipid (1.02%) contents intermediate between those of the two imported melania snails. The prominent fatty acids were 16:0 and 18:0 saturated fatty acids, 16:1n-7, 20:1n-11, 18:1n-9, 18:1n-7 and 16:1n-9 monounsaturated fatty acids, and 20:4n-6, 20:5n-3 (eicosapentaenoic acid, EPA), 22:2 non-methylene-interrupted diene (NMID), 18:3n-3, 18:2n-6 and 22:5n-3 (docosapentaenoic acid, DPA) polyunsaturated fatty acids (PUFA). The percentage of n-3 PUFA was higher in domestic (19.6%) and North Korean (imported, 19.5%) snails than in that from China (imported, 16.8%), while the n-6 PUFA level was higher in snails from China (21.2%) and domestic snails (14.7%) than in that from North Korea (9.87%). The total DMA, i.e., 18:0 DMA, 16:0 DMA and 18:1 DMA was 7.30, 7.27, and 4.86% domestic snail and those from China and North Korea, respectively. These results suggest that melania snail is a source of n-3 PUFA, and DMA derived from plasmalogen.