돼지생식기호흡기 증후군(PRRS) 바이러스 감염시킨 돼지 기관의 병리학적 관찰

조성환,김현수,윤원기,전무형,류시윤,박배근,손화영,김무강 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

The porcine reproductive and respiratory syndrome virus was inoculated into swine tracheal organ culture. The ballooning degeneration of the tracheal ciliated epithelium and a decrease of ciliary activity from the tracheal organs infected with PRRS virus were observed one day post-infection(PI). Tracheal epithelium showed degeneration and focal necrosis 2 days PI. Epithelial necrosis and loss of cilia and epithelial cells became more severe 3 and 4 days PI. An entire loss of cilia and epithelial cells were recognized 4 days PI, however, control tracheal organs were normal during the period of the experiment except a little decrease of ciliary activities with time..

한국 재래산양의 위장관에 분포하는 내분비세포의 면역조직화학적 연구

조성환,김무강,류시윤,허민도 대한해부학회 1992 Anatomy & Cell Biology Vol.25 No.1

젖소 足皮膚炎에 대한 오존수의 治療 效果

이수진,조성환,전무형,김덕환,박창식,한홍율,김명철 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

This study was carried out to determine therapeutic the effect of ozonated water therapy on bovine pododermatitis. In addition, bactericidal effect of ozonated water on etiological agent of bovine pododermatitis was examined. The pathohistological examination for the pododermatitis, according to application with ozonated water and ozone ointment was investigated. Thirty healthy cattle were divided two groups(each of 15) : control group (povidone group), treatment group (ozone solution group). Various parameters were evaluated in terms of the lameness score, swelling score, lesion score, WBC count, neutrophil count, pathohistological finding, and antimicrobial action. The decrease of lameness and lesion score were shown in hoof lesions on 14 days after application of ozonated water. Significant decrease of swelling was shown in hoof lesions on 14 days 1 Corresponding author after application of ozonated water(p < 0.01). In hematological findings, WBC count revealed values within normal range. The number of neutrophils was slightly higher than that of normal, however, this was improved on 14 days after application of ozonated water. In pathohistological findings, recovery was rapid macroscopically and microscopically in the treatment with ozonated water on the hoof lesions and ozonated water was effective. In antimicobial action, bactericidal effect was observed in treatment with ozonated water on the hoof lesions and ozonated water was effective.

국내분리 porcine reproductive and respiratory syndrome(PRRS) virus에 인공감염된 자돈에 관한 연구

장경수,조성환,권창희,전무형 충남대학교 수의과대학 동물의과학연구소 1995 動物醫科學硏究誌 Vol.3 No.-

The Korean isolates of porcine reproductive and respiratory syndrom(PRRS) virus, KPRRSV-1 and KPRRSV-2, were studied on the aspects of pathogenicity in the experimentally infected piglets. The piglets inoculated intranasally(in) with the virus at 10^4.5TCID_50/㎖(GroupⅠ) showed clinical signs of milder fever, respiratory disorders, anorexia, growth retardation and transient leucopenia. It was evident that the virus could be transmitted by contract. Histopathologically interstitial pneumonitis was markedly noted in the lung of all infected piglets. In studies on antibody responses of the experimental animals, seroconversions were noted at 6∼9 days pe in GroupⅠ(in inoculated) and at 12∼15 days pe in Group Ⅱ(contract). The antibody titers at 30 days pe were ranged from 256 to over 1024. By avidin-biotin immunofluorescence(ABIF) assay, the PRRS virus antigens were detected in bronchi, bronchiol and alveolar epitheliums and interalveolar septa of the experimentally infected piglets. The viruses were recovered from the tissues of lung and spleen of the infected piglets.

나이에 따른 대식세포의 IL-6 생산양상의 변화

윤원기,김무강,조성환,류시윤 충남대학교 수의과대학 동물의과학연구소 1994 動物醫科學硏究誌 Vol.2 No.-

The present study was designed to observe the age-related changes in the IL-6 production capabilities of the peritoneal macrophages. Pertioneal macrophages were collected from adult mature (13-20 weeks of age) and aged BALB/c mice (60-70 weeks of age). Macrophages were cultured in the presence of endotoxin (lipopolysaccharide lug/ml, Escherichia coli serotype 0111:B4). In aged mice, IL-6 levels in the culture supernatants were seemed to be relative high than that in adult mature mice.

인천 인현동 호프집 화재 피해자 분석

최정태,안무업,안희철,최영미,정재봉,서정열,유기철,이삼우,박석현,조준휘,김성환,김아진,송근정 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4

Purpose : This study was conducted to develop field triage, transportation, distribution, and prehospital care at a fire disaster by analyzing the victims of the fore that broke out at a bar in Incheon. Method : We analyzed the cases of the victims of a fire in Incheon in Oct. 1999. We determined the primary care hospital, the arrival time, the burn size, the outcome, and the injury type from the medical records, the concerned organ records, and interviews with concerned persons. Result : The total number of victims was 137: 56 prehospital deaths, 1 hospital death, and 80 survivals. The Pearson correlation coefficient between the burn size and the severity was -0.175. There were 121(89.6%) cases of inhalation injury, 59 (43.7%) cases of flame burns, 66 (48.9%) cases of hypoxic brain damage, and 16 (11.9%) cases involving other types of injury. Conclusion : The causes of death of the fire victims were inhalation injury and hypoxic brain damage due to CO poisoning and other toxic inhalants. We propose the use of a simple triage and rapid treatment(START) system and a reassessment the delayed category in fire disasters.

韓國人 手掌皮膚 Meissner 小體의 量的 變化에 關한 硏究

金弘善,金武剛,趙聲煥,柳時潤 충남대학교 의과대학 지역사회의학연구소 1983 충남의대잡지 Vol.10 No.2

In order to observe the quantitative changes in Meissner's tactile corpuscles caused by aging and in different areas, 3×5 ㎟ punch excision specimens in the palm of the right and left hand from the 46 cadavers (male 35, female 11) were collected, sectioned serially, stained by the methylene blue and observed with light microscope and then analyzed a numerical changes in the palm of the right and left hand and in the site 6 and 7 in the palm of the hand. The results are summerized as follows: 1. The mean concentration of Meissner's corpuscles in the palm of the right hand was 2.92 per square millimeter and in the palm of the left hand was 5.17. There was appeared the mean concentration of Meissner's corpuscles in the palm of the hand was greater in the left than in the right. 2. In the site 6, the mean concentration of Meissner's corpuscles was 3.93 per square millimeter in the age group 21 to 50 years, 2.96 in the 51 to 60 years, 2.52 in the 61 to 83 years. There was a decrease in the mean concentration of Meissner's corpuscles with increasing age. 3. In the site 7, the mean concentration of Meissner's corpuscles was 5.51 per square millimeter in the age group 21 to 50 years, 4.98 in the 51 to 60 years, 4.94 in the 61 to 78 years. Also, there was a decrease in the mean concentration of Meissner's corpuscles with increasing age. 4. In the site 6, the mean concentration of Meissner's corpuscles in the palm of the right hand was 3.57 per square millimeter in the age group 21 to 50 years, 2.74 in the 61 to 78 years and in the palm of the left hand was 3.07 in the 21 to 60 years, 2.24 in the 61 to 83 years. There was appeared the mean concentration of Meissner's corpuscles in the site 6 of the palm was greater in the right than in the left. 5. In tie site 7, the mean concentration of Meissner's corpuscles in the palm of the right hand was 5.12 per square millimeter in the age group 21 to 78 years and in the palm of the left hand was 5.13 in the 21 to 78 years. There was appeared the mean concentration of Meissner's corpuscles in the site 7 of the palm was a little greater in the left than in the right.

젖소 足皮膚炎에 대한 오존연고의 治療 效果

이수진,전무형,조성환,김덕환,박창식,김명철 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

Thirty dairy cows with pododermatitis were selected and treatment effect of ozone ointment for bovine pododermatitis was investigated. In addition, bactericidal effect of ozone ointment on etiological agent of bovine pododermatitis was evaluated. The pathohistological examination for the pododermatitis, according to application with ozone ointment was investigated. Thirty dairy cows were divided two groups: control group(vaseline group: 15 cows), treatment group(ozone ointment group: 15 cows). Various parameters were evaluated in terms of the lameness score, swelling score, lesion score, WBC, neutrophil, pathohistological finding, and antimicrobial action. As compared with vaseline group, ozone ointment group revealed significant decrease of lameness (p<O.05), swelling (p<O.O1) and lesion score (p<O.05) were shown in hoof lesions on 14 days after application. In hematological findings, WBC count revealed slightly high values within normal range before treatment, however, this was I Corresponding author improved on 14 days after application of ozone ointment. The number of neutrophils was slightly higher than that of normal, however. this was improved on 14 days after application of ozone ointment. In pathohistological findings, normal dermal tissue was found in tissues with pododermatitis on 14 days after application of ozone ointment. In antimicobial action, marked decrease rate of bacteria was observed in feet of all cases treated with ozone ointment. The decreasing rate of bacteria in anaerobic culture was higher than that in aerobic culture.

절수에 의한 mongolian gerbil의 체중 변화에 관한 연구

이경열,송치원,김무강,조성환,류시윤,김상근,박배근 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

Recently, the mongolian gerbils have been used in neurology and the study of water metabolism because of their easy onset of epileptic seizure and typical resistance to water deprivation. The sebaceous glands which are under the control of the gonadal hrmones are differently developed from the other laboratory animals. However the physiological data on the changes of body weight following water deprivation in mongolian gerbils is not available until now. In order to investigate the effect of the water deprivation on the body weight, the animals were deprived of water for 15 days. The body weights were measured daily by the torsion balance. The mean, the standard deviation, the standard error and the coefficient of variance of the body weights were calculated. The correlation was examined. The potential regression equation was y = 61.5458 - 2.0533 * x + 0.0487 * x^2. The regression coefficient was 0.993. The results obtained were as follows : 1. The body weights of the mongolian gerbils were continuously decrased from the 1st day to the 15th day after the water deprivation. 2. The mean body weight on the water deprivation was 63.0±7.00g and that at the 15th day was 41.4 ± 5.42g. 3. The coefficients of the variance showed the highest (13.09) at the 15th day and the lowest (10.10) at the 10th day. the coefficients of the variance kept decreasing until the 10th day and thereafter gradually increased until the 15th day. 4. The changing rate of the body weight kept continuously decreasing from the 1st day to the 15th day showing the daily loss of 1.0 - 3.3%. 5.The deviations (M - QBW) between the measured values (m) and the theorical values (QBW) using the regression equation showed the highest (1.494) at the 1st day and the lowest (0.053) at the 13th day. 6. The sum of the devitions was 0.179 and that of the deviation square was 1.15. The sum of the deviation rates between the mesaured body weights and the theorical ones was 0.484. 7. The daily body weight loss showed the highest at the 1st day by 2.053g and the lowest at the 15th day by 0.591g. 8. The specific body weight changing rates which were the rate of the body weight changing velocity to the changed body weight were decreased from 0.033% at the 1st day to 0.014% at the 15th day.

농장 사육되는 타조에서 나타난 다리 기형의 증례 보고

최종윤,손화영,전무형,조성환,김혜성,류시윤,박배근,이영원,윤원기 충남대학교 수의과대학 동물의과학연구소 2001 動物醫科學硏究誌 Vol.9 No.-

Two farmed young ostrich chicks were applied to pathology laboratory at Chungnam National University. Grossly, one ostrich showed rotated tibiotarsus, the other showed rolled toes. Case 1 ostrich was hypertrophy of fibualis longus and tibial cranialis tendon and gastrocnemius tendon in crus, inflammation of flexor digitorum longus. In histopathological views, myocytes of fiburalis longus were showed hypertrophy and hyperplasia. It is not found any specific feature in X-ray of the case 2 ostrich. Limb deformities were detected frequently in farmed ostrich chicks in 2- to 3 weeks old. Limb deformities were an important cause of mortality in farmed ostrich chicks during the first 10weeks after hatch. Cause of limb deformities were considered a nutrition problem.