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Transformation of Wheat Immature Embryos by Particle Bombardment
Wu Li-Min,Wei Yu-Ming,Zheng You-Liang The Korean Society of Plant Biotechnology 2005 Plant molecular biology and biotechnology research Vol.7 No.2
The objective of this study was to identify the major parameters controlling DNA delivery by particle bombardment to immature embryos of Chinese spring wheat (Triticum aestivum L.). Efficiency of DNA (uidA gene) delivery was assessed by transient GUS (${\beta}$-glucuronidase) expression in bombarded tissues. Of the parameters analyzed, acceleration pressure, bombardment distance, chamber vacuum pressure, bombardment times, osmotic conditioning of culture had a remarkable influence on transient gene expression. A bombardment procedure suitable for Chinese spring wheat cultivars was developed which allowed high-efficiency DNA delivery combined with reduced damage to target tissues. The high efficiency made the system practical for wheat genetic transformation research and accelerating wheat breeding programs.
( So Min Lyu ),( Ju Yeon Wu ),( Ji Yeon Byun ),( Hae Young Choi ),( Sang Hee Park ),( You Won Choi ) 대한피부과학회 2016 Annals of Dermatology Vol.28 No.5
Background: The role of the phosphatidylinositol-3 kinase signaling pathway in the development of acral melanoma has recently gained evidence. Phosphatase and tensin homologue (PTEN), one of the key molecules in the pathway, acts as a tumor suppressor through either an Akt-dependent or Akt-independent pathway. Akt accelerates degradation of p53. Objective: We assessed the expression of PTEN, phospho- Akt (p-Akt), and p53 by immunohistochemistry in benign acral nevi, acral dysplastic nevi, and acral melanomas in the radial growth phase and with a vertical growth component. Methods: Ten specimens in each group were included. Paraffin-embedded specimens were immunostained with antibodies for PTEN, p-Akt, and p53. We scored both the staining intensity and the proportion of positive cells. The final score was calculated by multiplying the intensity score by the proportion score. Results: All specimens of benign acral nevi except one showed some degree of PTEN-negative cells. The numbers of p-Akt and p53-positive cells were higher in acral dysplastic nevi and melanoma than in benign nevi. P-Akt scores were 1.7, 1.8, 2.6, and 4.4, and p53 scores were 2.0, 2.1, 3.8, and 4.1 in each group. PTEN and p-Akt scores in advanced acral melanoma were higher than in the other neoplasms. Conclusion: The expression of PTEN was decreased and the expression of p-Akt was increased in acral melanoma, especially in advanced cases. The PTEN-induced pathway appears to affect the late stage of melanomagenesis. Altered expression of p-Akt is thought to be due to secondary changes following the loss of PTEN. (Ann Dermatol 28(5) 548∼554, 2016)
Sclerodermatous chronic graft‐versus‐host disease induced by host T‐cell‐mediated autoimmunity
Lee, You Jeong,Min, Hye Sook,Kang, Eun Ha,Park, Hyo Jin,Jeon, Yoon Kyung,Kim, Ju Hyun,Wu, Hong Gyun,Lee, Eun‐,Bong,Park, Chung‐,Gyu,Yoon, Sung‐,Soo,Park, Seong Hoe,Jung, Kyeong Cheon Nature Publishing Group 2012 Immunology and cell biology Vol.90 No.3
<P>Despite a long‐standing hypothesis that chronic graft‐versus‐host disease (cGVHD) is an autoimmune disorder, most mouse models of cGVHD have been developed on the assumption that donor T cells are essential for its development. Here we show that cGVHD may be caused by autoreactive host T cells in mice that have been lethally irradiated and grafted with T‐cell‐depleted allogeneic bone marrow cells. In this chimera, host T cells derived from radioresistant intrathymic T‐cell precursors caused dermal fibrosis and periportal inflammation, without the requirement for donor T cells. The lack of host DCs within the thymus after high‐dose irradiation allowed autoreactive host T cells to escape thymic negative selection. Moreover, the homeostatic expansion of these T cells may augment their autoreactivity. These findings indicate that host T‐cell‐mediated cGVHD is an autoimmune process that occurs following the grafting of T‐cell‐depleted BM cells into hosts with functioning thymuses. We propose, based on the present data, that host T‐cell‐dependent autoimmunity is a potential mechanism by which cGVHD is induced.</P>
Trajectory planning of multi-degree-of-freedom robot with coupling effect
Kunming Zheng,You-Min Hu,Bo Wu 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.1
For a multi-degree-of-freedom (MDOF) robot with flexible components, vibration errors can easily occur during operation. Thus, the position of the end effector inevitably deviates from its desired value and causes failure of the precise trajectory tracking task. To solve this problem, an MDOF KUKA robot is introduced in the present study. We propose a new methodology to investigate the dynamic coupling effect and trajectory planning for the robot. The dynamic coupling effect index is defined for the first time, thereby providing a theoretical basis for the trajectory planning. Moreover, a new trajectory plan is adopted to reduce the vibration errors caused by the coupling effect in the Cartesian coordinate and joint coordinate systems. The advantages of the proposed methodology in improving accuracy and stability are validated by experiments. In addition, the chaos phenomenon is observed, which is the focus of our future study.
Wen-Ting Hsiao,Hui-Min Su,Kuan-Pin Su,Szu-Han Chen,Hai-Ping Wu,Yi-Ling You,Ru-Huei Fu,Pei-Min Chao 한국영양학회 2019 Nutrition Research and Practice Vol.13 No.4
BACKGROUND/OBJECTIVES: Docosahexaenoic acid (DHA), an n-3 long chain polyunsaturated fatty acid (LCPUFA), is acquired by dietary intake or the in vivo conversion of α-linolenic acid. Many enzymes participating in LCPUFA synthesis are regulated by peroxisome proliferator-activated receptor alpha (PPARα). Therefore, it was hypothesized that the tissue accretion of endogenously synthesized DHA could be modified by PPARα. MATERIALS/METHODS: The tissue DHA concentrations and mRNA levels of genes participating in DHA biosynthesis were compared among PPARα homozygous (KO), heterozygous (HZ), and wild type (WT) mice (Exp I), and between WT mice treated with clofibrate (PPARα agonist) or those not treated (Exp II). In ExpII, the expression levels of the proteins associated with DHA function in the brain cortex and retina were also measured. An n3-PUFA depleted/replenished regimen was applied to mitigate the confounding effects of maternal DHA. RESULTS: PPARα ablation reduced the hepatic Acox, Fads1, and Fads2 mRNA levels, as well as the DHA concentration in the liver, but not in the brain cortex. In contrast, PPARα activation increased hepatic Acox, Fads1, Fads2, and Elovl5 mRNA levels, but reduced the DHA concentrations in the liver, retina, and phospholipid of brain cortex, and decreased mRNA and protein levels of the brain-derived neurotrophic factor in brain cortex. CONCLUSIONS: LCPUFA enzyme expression was altered by PPARα. Either PPARα deficiency or activation-decreased tissue DHA concentration is a stimulus for further studies to determine the functional significance.