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( So Min Lyu ),( Ju Yeon Wu ),( Ji Yeon Byun ),( Hae Young Choi ),( Sang Hee Park ),( You Won Choi ) 대한피부과학회 2016 Annals of Dermatology Vol.28 No.5
Background: The role of the phosphatidylinositol-3 kinase signaling pathway in the development of acral melanoma has recently gained evidence. Phosphatase and tensin homologue (PTEN), one of the key molecules in the pathway, acts as a tumor suppressor through either an Akt-dependent or Akt-independent pathway. Akt accelerates degradation of p53. Objective: We assessed the expression of PTEN, phospho- Akt (p-Akt), and p53 by immunohistochemistry in benign acral nevi, acral dysplastic nevi, and acral melanomas in the radial growth phase and with a vertical growth component. Methods: Ten specimens in each group were included. Paraffin-embedded specimens were immunostained with antibodies for PTEN, p-Akt, and p53. We scored both the staining intensity and the proportion of positive cells. The final score was calculated by multiplying the intensity score by the proportion score. Results: All specimens of benign acral nevi except one showed some degree of PTEN-negative cells. The numbers of p-Akt and p53-positive cells were higher in acral dysplastic nevi and melanoma than in benign nevi. P-Akt scores were 1.7, 1.8, 2.6, and 4.4, and p53 scores were 2.0, 2.1, 3.8, and 4.1 in each group. PTEN and p-Akt scores in advanced acral melanoma were higher than in the other neoplasms. Conclusion: The expression of PTEN was decreased and the expression of p-Akt was increased in acral melanoma, especially in advanced cases. The PTEN-induced pathway appears to affect the late stage of melanomagenesis. Altered expression of p-Akt is thought to be due to secondary changes following the loss of PTEN. (Ann Dermatol 28(5) 548∼554, 2016)
P066 : Analysis of nuclear PTEN expression in acral melanocytic lesions
( So Min Lyu ),( Ji Yeon Byun ),( Hae Young Choi ),( You Won Choi ) 대한피부과학회 2013 대한피부과학회 학술발표대회집 Vol.65 No.2
Background: The PTEN tumor suppressor gene is a negative regulator of the PI3K-AKT pathway through encoding a lipid and protein phosphatase. Allelic loss or ectopic expression of PTEN has been described in a variety of cell lines including melanoma. Objectives: We assessed the proportion and the pattern of nuclear PTEN loss by immunostaining in acral junctional nevi, acral dysplastic nevi, acral melanomas and advanced acral melanomas. Methods: 10 Patients in each group who visited our clinic from 2005 Jan to 2013 Jun were included. All paraffinembedded biopsy specimens were immunostained with anti- PTEN antibody and examined by two dermatologists. Results: The proportion of nuclear PTEN-loss melanocytes were 8.9% in acral junctional nevi, 22% in acral dysplastic nevi, 21% in acral melanomas and 53% in advanced acral melanomas. Melanocytes in acral junctional nevi showed uniformly strong nuclear PTEN staining. In acral dysplastic nevi and acral melanomas, randomly scattered PTEN-lost melanocytes were observed. In contrast, there were patchy areas of PTEN loss in advanced acral melanoma. The result is on the same way with previous studies of melanomas of other body sites, but the proportion of PTEN-positive cells looks slightly higher in our study. Conclusion: Nuclear PTEN expression were uniformly positive in acral junctional nevi, randomly lost in ~20% of cells in acral dysplastic nevi and thin acral melanomas, and showing patchy loss in half of melanoma cells in advanced acral melanomas.