Label-free, microfluidic separation and enrichment of human breast cancer cells by adhesion difference

Kwon, Keon Woo,Choi, Sung Sik,Lee, Sang Ho,Kim, Byungkyu,Lee, Se Na,Park, Min Cheol,Kim, Pilnam,Hwang, Se Yon,Suh, Kahp Y. GENERAL AND APPLIED CHEMISTRY JOURNALS 2007 LAB ON A CHIP Vol.7 No.11

<P>A label-free microfluidic method for separation and enrichment of human breast cancer cells is presented using cell adhesion as a physical marker. To maximize the adhesion difference between normal epithelial and cancer cells, flat or nanostructured polymer surfaces (400 nm pillars, 400 nm perpendicular, or 400 nm parallel lines) were constructed on the bottom of polydimethylsiloxane (PDMS) microfluidic channels in a parallel fashion using a UV-assisted capillary moulding technique. The adhesion of human breast epithelial cells (MCF10A) and cancer cells (MCF7) on each channel was independently measured based on detachment assays where the adherent cells were counted with increasing flow rate after a pre-culture for a period of time (<I>e.g.</I>, one, two, and four hours). It was found that MCF10A cells showed higher adhesion than MCF7 cells regardless of culture time and surface nanotopography at all flow rates, resulting in label-free separation and enrichment of cancer cells. For the cell types used in our study, an optimum separation was found for 2 hours pre-culture on the 400 nm perpendicular line pattern followed by flow-induced detachment at a flow rate of 200 µl min<SUP>−1</SUP>. The fraction of MCF7 cells was increased from 0.36 ± 0.04 to 0.83 ± 0.04 under these optimized conditions.</P> <P>Graphic Abstract</P><P>A label-free microfluidic cell separation and enrichment device is presented here by using cell adhesion as a physical marker. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b710054j'> </P>

난치성 백반증에 대한 흡입물집을 이용한 비배양 표피세포이식술 20례

배정민 ( Jung Min Bae ),정한미 ( Han Mi Jung ),이한나 ( Han Na Lee ),이로우 ( Ro Woo Lee ),은성혜 ( Sung Hye Eun ),권혁선 ( Hyuck Sun Kwon ),이지혜 ( Ji Hae Lee ),김경문 ( Gyong Moon Kim ) 대한피부과학회 2018 대한피부과학회지 Vol.56 No.7

Background: As nonsurgical interventions for vitiligo are not always successful, various surgical modalities have been used in patients with refractory vitiligo. Of these, non-cultured epidermal suspension transplantation (NCES) was recently introduced to treat large recipient sites using cells from small donor tissue. Objective: We assessed the effectiveness and safety of NCES as a surgical treatment for patients with refractory vitiligo. Methods: We retrospectively reviewed 20 cases in 17 patients (11 females; median age 25 years) who underwent NCES from July 2015 through March 2018. Suction blisters (20 mm in diameter) were collected from the patient’s inner thigh at a donor-to-recipient area ratio of 1:5. After the addition of 5 mL recombinant trypsin solution to the suction blisters, followed by incubation at 37°C for 60 min, epidermal cells were manually scraped off the blister surface, and epidermal cell suspension was obtained by centrifugation at 1,500 RPM for 5 min. The suspension was applied to the vitiligo regions after epidermal ablation of those regions. Phototherapy resumed 1 month later. Treatment success was defined as ≥75% repigmentation of the surgical site, and all adverse events were noted. Results: Overall, 85.0% of cases (17/20) exhibited treatment success. Adverse events included hyperpigmentation (20%) and surgical site infection (5%), but the treatment was tolerable in all cases. Conclusion: NCES is a reliable surgical option for patients with vitiligo refractory to nonsurgical treatment. Large areas of vitiligo can be treated by NCES, and use of this technique should be encouraged in Korea. (Korean J Dermatol 2018;56(7):426∼432)

수변공원과 체육공원의 중요 속성

홍성권 ( Sung Kwon Hong ),이상우 ( Sang Woo1 Lee ),이민우 ( Min Woo Lee ),이상민 ( Sang Min Lee ) 한국환경복원기술학회 2007 한국환경복원기술학회지 Vol.10 No.6

비만학생들의 운동후 회복시 초과 산소소비량 및 지속시간에 관한 연구

박철호,권민호,고봉민,박은경,우상헌 東亞大學校附設스포츠科學硏究所 1999 스포츠科學硏究論文集 Vol.17 No.-

The purpose of this study was to examine the effect of two different intensity of continuous exercise on magnitude of excess post-exercise oxygen consumption(EPOC), energy consumption following a 20min treadmill exercise. The subjects of this study were eight super-obesity in middle school students who were above 13 years old. subjects participated in two different exercise intensity to measure respiratory gas, blood lactate concentration and eardrum temperature. The results of this study were as follows: 1. The Energy consumption of the high intensity (80% VO₂ max) exercise group (264.68 35.60cal) was greater than that of the low intensity (50%) exercise group(172.01 20.39 Cal)(p<.001). 2. The EPOC of the high intensity exercise group (22.36+ 6.82Cal ) was greater than that of the low intensity exercise group(15.39+5.27 Cal)(p<.001). 3. The EPOC duration of th high intensity exercise group(28.36=3.56min) was longer than that of the low intensity exercise group(17.23+1.25min)(p<.001) 4. The blood lactate concentration, respiratory exchange ratio and temperature of the high intensity exercise group were greater than those of the low intensity exercise group(p<.05,p<.01). EPOC and duration were increase with 80%VO₂max, 80% VO₂max as intensity exercise got higher this also showed that it had something to do with lactate concentration in blood and increase of body temperature in the conclusion. We considered that there was a difference in using methods of energy, as there was a similar difference in RER. We though this following with oxygen consumption after exercise, we could clarify that high intensity exercise was better than lower intensity exercise in sametime for those who are obese especially adolescents to maintain their weights on the aspect of effect.

금불초 종(種) 및 개화시기에 따른 금불초 꽃 추출물의 항산화 효능

권순식 ( Soon Sik Kwon ),전소하 ( So Ha Jeon ),전지민 ( Ji Min Jeon ),천종우 ( Jong Woo Cheon ),박수남 ( Soo Nam Park ) 대한화장품학회 2013 대한화장품학회지 Vol.39 No.3

본 연구에서는 금불초(Inula britannica) 종(種) 및 개화시기에 따른 금불초 추출물의 항산화 효능을 알아보았다. 이들 추출물의 free radical 소거활성을 살펴본 결과, 만개한 금불초 꽃(I. britannica var. chinensis) 추출물의 경우 500 μg/mL의 농도에서 79.89%의 free radical 소거활성을 보였으나, 금불초 유사종인 가는 금불초(I. britannica var. linariaefolia Regel), 가지 금불초(I. britannica var. ramosa) 및 버들 금불초(I. salicina var. asiatica)의 꽃 추출물의 경우 free radical 소거활성이 거의 나타나지 않았다. 또한 꽃이 만개하였을 경우 꽃 추출물 분획에서는 93.62%의 free radical 소거활성을 보였으며, 봉우리 추출물 분획은 43.28%, 낙화추출물 분획은 14.11%를 나타냈다. 금불초의 종 및 개화시기를 선정 후, 추출용매, 온도, 시간을 조절하여 최적의 추출조건을 확립하였다. 그 결과, 65 ℃ 에탄올 추출물에서 가장 높은 DPPH free radical 소거활성이 나타났으며, 시간에 따른 유의적 차이는 없는 것으로 나타났다. 만개한 금불초 꽃 추출물에 대하여 rose-bengal로 증감된 사람 적혈구의 광용혈 실험에서 세포보호효과를 측정한 결과, 5 ~ 50 μg/mL의 범위에서 농도 의존적으로 세포 보호 효과를 나타내었다. 특히 50 μg/mL의 농도에서 τ50이 116.1 min으로 비교물질인 (+)-α-tocopherol에 비해 1.58배 더 큰 세포 보호활성이 있음을 알 수 있었다. HPLC로 금불초 꽃 추출물을 분석한 결과 flavonoid의 일종인 quercetin이 다량 함유되어 있는 것을 확인하였다. 이상의 결과들은, 65 ℃ 에탄올로 추출한 만개한 금불초 꽃 추출물의 경우 다량의 quercetin을 함유하며, 그로 인하여 free radical 소거활성 및 ROS에 대항하여 세포막을 효과적으로 보호함으로써 태양 자외선에 노출된 피부를 보호하는 항산화제로서 작용할 수 있을 것으로 사료되며, 기능성 화장품 원료로서 응용 가능성이 있음을 시사한다. In this study, antioxidative effects of the extracts of different species and flowering periods of Inula britannica were investigated. According to the free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity of the extracts, The I. britannica var. chinensis flower extract (500 μg/mL) was measured in a 79.89% free radical scavenging activity, but the flower extracts of similar species (I. britannica var. linariaefolia Regel, I. britannica var. ramosa, I. salicina var. asiatica) did not show any effect on the free radical scavenging activity. The effects of the free radical scavenging activity of I. britannica var. chinensis flower extracts were exhibited in the order of full bloom (93.68%), bud (43.28%), and fallen blossom (14.11%). Next, we established optimum condition of extract solvent, temperature, extraction time. The extract from ethanol at 60 ℃ showed the most free radical scavenging activity among other conditions and extraction time not relevant in free radical scavenging activity. The protective effects of the extract of I. britannica var. chinensis flower on the photohemolysis of human erythrocytes by using rose bengal were increased in a concentration-dependent manner (5 ∼ 50 μg/mL). In particular, the extract in 50 μg/mL concentration exhibited better protective activity (τ₅₀ = 116.1 min) than (+)-α-tocopherol (τ₅₀ = 73.44 min), which is a known lipophilic antioxidant. Principle component of I. britannica var. chinensis flower was identified as quercetin of flavonoids by high-performance liquid chromatography (HPLC). These results indicate that the extract of I. britannica var. chinensis flower can function as antioxidants in biological systems, particularly skin exposed to UV radiation by scavenging free radical and ¹O₂, and protect cellular membranes against ROS. It is concluded that the antioxidative effects of the extract of I. britannica var. chinensis flower could be applicable to functional cosmetics.

향류마치스 효과를 갖는 새로운 히스톤 Hl 단백질 (p961)의 횐쥐와 토끼에 대한 약물동태

우수경(Su Kyung Woo),윤민혁(Min Hyuk Yun),이재홍(Jae Heung Lee),권광일(Kwang Il Kwon) 대한약학회 2001 약학회지 Vol.45 No.4

A purified histone Hl protein, p961, which plays a role in mediating the condensation of DNA into chromatin, was recently proved as an arthritis-suppressing agent in the mouse CIA model. The pearmacokinetics of p961 was carried out in rats and rabbits. The rat's blood, bile and urine samples were semially collected from the femoral vein, common bile duct, and bladder respective1y; after bolus I.v. injection at low (10 mghg) and high (30 mg/mg) doses. The rabbit's blood samples were also collected from the marginal ear vein after bolus I.Y. injection at a dose 10 mg/kg. P961 and its major metabolite in the physiological samples were analyzed by reverse-phase HPLC using a Vydac C4 protein column and a multistep water-acetonitrile gradient containing 0.24% trifluoroacetic acid. The major pharmacokinetic parameters (AUC, Cmax, MRT t1/2, Vss and Cl) were estimated from the time course of plasma p961 and metabolite concentrations using WinNonlin. A two- compartment model was chosen for p961 as the most appropriate pharmacokinetic model. After I.v, injection of p961 at doses of 10 mghg and 30 mg/kg, more than 80% of p961 was removed rapidly from the plasma within 15 min. The plasma half-life of p961 in rats and rabbits was found not to exceed 12 min. p961 (22448.9 mot wt) was rapidly cleaved to 21612 mol wt fragment and the breakdown product appeared rapidly in the circulation with no lag phase. P961 and metabolite were not detected in rat urine and bile.

도심 수목원에서의 경관감상과 산책이 인체의 생리적 안정에 미치는 영향

박범진 ( Bum Jin Park ),가재남 ( Jae Nam Ka ),이민선 ( Min Sun Lee ),김선아 ( Seon A Kim ),박민우 ( Min Woo Park ),최윤호 ( Yoon Ho Choi ),정다워 ( Da Wou Joung ),권치원 ( Chi Weon Kwon ),염동걸 ( Dong Geol Yeom ),박순주 ( Soon Jo 한국산림과학회 2014 한국산림과학회지 Vol.103 No.4

This research was conducted to investigate the impact of viewing scenery and walking in the urban forest onphysiological relaxation of humans. The experiment was conducted in Hanbat Arboretum located in Daejeon, and thecontrol experiment was conducted in front of Daejeon City Hall. The subjects that participated in the experimentcomprised 24 Korean male university students in their 20s (average age, 21.1±2.5 years), participated in the experimentas the subject, and these subjects were classified into three groups divided into three locations such as the pine forest inArboretum, the pond in Arboretum, and the city. The subjects sat down and viewed scenery for 10 min, and they thenwalked for 15 min. Further, physiological changes were measured using indicators such as heart rate variability (HRV),blood pressure, and pulse rate. As a result, when subjects viewed scenery and walked in the urban forest, a statisticallysignificant increase in the high frequency (HF) power of HRV and decrease in systolic pressure was observed comparedwith when subjects viewed scenery and walked in the city. Through this research, it was found that activities like viewingscenery or walking in Arboretum of the city are effective in increasing the physiological relaxation of the city residents.

태권도 돌려차기 시 기술 수준에 따른 몸통, 골반, 지지 다리의 운동학적 변인 비교

김재웅 ( Kim Jae-woong ),한기훈 ( Han Ki-hoon ),김제민 ( Kim Je-min ),이상우 ( Lee Sang-woo ),권문석 ( Kwon Moon-seok ),터틀노엘 ( Noelle J. Tuttle ) 국기원 2018 국기원태권도연구 Vol.9 No.1

이 연구의 목적은 태권도 돌려차기 시 기술 수준에 따른 몸통, 골반, 지지 다리의 운동학적 변인을 비교 분석하는 데 있다. 본 연구를 위해 블랙 벨트 소지자로서 기술 수준에 따라 중급자10명, 상급자 9명이 실험에 참여하였다. 3차원 동작 분석 시스템을 사용하여 골반, 몸통, 지지다리의 방향각을 산출하였다. Start, Toeoff, MKF, Max, Impact, Min에서 그룹 간의 평균 방향각을 비교하기 위하여 독립표본 t-검정을 하였다. MKF, Impact, Min에서 골반의 좌측 기울어짐 각도가 그룹 간의 유의한 차이가 나타났다. Start와 Max에서 지지 다리의 엉덩관절 굴곡 각도와 MKF에서 지지 다리의 엉덩관절 외전 각도 사이에 유의한 차이가 있었다. 이상의 결과를 볼 때, 상급자들은 푸쉬단계에서 엉덩관절의 굴곡 각도가 증가 되었고, MKF에서는 외전 각도가 감소하였다. 또한, 스트라이크 단계에서 골반의 좌측 기울어짐 각도는 감소하였다. The study purpose was to investigate skill level differences on trunk, pelvis, and supporting leg kinematics in a Taekwondo roundhouse kick. 10 intermediate and nine expert male black-belt holders executed roundhouse kicks at a target. Orientation angles of the pelvis, trunk, and supporting -thigh, -shank, and -foot were obtained through a three-dimensional video motion analysis. Orientation angles at Start, Toeoff, Maximum Knee Flexion(MKF), Impact, Maximum angle(Max), and Minimum angle(Min) were compared between groups using independent t-tests(p<.05). Significant differences between groups were found in the pelvis left lateral tilted position at MKF, Impact, and Min; significant differences were also found in the supporting hip joint flexed position at Start and Max, and abducted position at MKF. It was concluded that higher performers increased the hip joint flexion angle in the Push phase, decreased abduction angle at MKF, and decreased the pelvis left tilted angle in the Strike phase.

최근 6년간 연세의료원에서 경험한 한국인 안면골 골절에 대한 임상적 연구

권준호,이충국,이의웅,민우석,윤중호,박형식 大韓顎顔面成形外科學會 1989 Maxillofacial Plastic Reconstructive Surgery Vol.11 No.1

This is a series of continuing research on facial bone fractures of Koreans worked by Dept. of Oral and Maxillofacial surgery, Dental College of Yonsei University, Seoul, Korea, since 1972. The study was based on a series of 630 patients with facial bone fractures treated as in patient at Yonsei Medical Center, Yonsei University, during the period of Jan. 1982 through Dec, 1987. The results obtained are as follows: 1. The ratio of Men to Women was 4.3 : 1, and admissions for facial bone fractures have been increased year after 1984. 2. The age frequency was highest in the third decade(38.3%). and fourth, second, fifth decade in orders. 3. The traffic accident was the most frequent cause of facial bone fractures(51.3%). 4. The most common location of facial bone fractures was the Mandible(35.3%), and Zygoma complex(29.8%), Nasal bone(15.0%), Maxilla(11.0%) were next in order of frequency. 5. In 291 patients of Mandible fractures, 226(77.7%) had fractures only in Mandible and 65(22.3%) had another facial bone fractures. The most frequent fracture site of mandible was the Symphysis(43.0%) and Angle(22.4%), and Simple fracture was the most frequent in type of fracture(66.9%). Intermaxillarty fixatin & Open reduction was major method of treatment(36.9%). 6. In 394 patients of Midface fractures 323(82.0%) had fractures only in Midface and 71(18.0%) had another facial bone fractures. The most frequent site of Midface fractures was zygoma complex & zygomatic arch(42.7%), and Simple fracture was the most common type of fractures. Observation(Maxilla :44.2%, Zygoma :51.0%) and Open Reduction(Maxilla :20.0%. Zygoma :23.5%) were the major method of treatment. 7. The frequency of Nasal bone fracture was about 1/5 of Midface fractures, and Closed Reduction(42.2%) was the major method of treatment. 8. The complication was reported in only 16 patients, and Malunion was the major complication. 9. Head(44.4%), Lower extrimities(14.5%) and Eye(12.3%) were injured commonly with facial bone fractures. 10. The elapsed time from injury to hospital was within 24 hours in 73.8% of patients, however 15.5% of patients arrived the hospital 72 hours after injury.

토양 과습 조건하에서 수수의 생육특성

윤민헌,권수정,주영환,최주영,우선희 忠北大學校 農業科學硏究所 2021 農業科學硏究 Vol.37 No.2

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