가압가열 및 Microwave 처리가 생면의 품질에 미치는 영향

박시우,김꽃봉우리,김민지,강보경,박원민,김보람,박홍민,최정수,최호덕,안동현,Bark, Si-Woo,Kim, Koth-Bong-Woo-Ri,Kim, Min-Ji,Kang, Bo-Kyeong,Pak, Won-Min,Kim, Bo-Ram,Park, Hong-Min,Choi, Jung-Su,Choi, Ho-Duk,Ahn, Dong-Hyun 한국식품영양학회 2013 韓國食品營養學會誌 Vol.26 No.4

중력분을 이용하여 제면한 후 가압가열, microwave 및 가압가열과 microwave 병행 처리한 후, 생면 및 삶은 면의 품질에 미치는 영향에 대하여 알아보았다. 생면의 pH는 microwave 1 min 처리구에서 무처리구와 유의적인 차이를 보이지 않았으며, 수분 함량은 autoclave 50 min 처리구에서 무처리구와 유의적인 차이를 보이지 않았고, 그 외의 처리구에서는 모두 수분 함량이 감소함을 확인하였다. 면의 색도 측정 결과는 명도는 무처리구에 비해 모든 처리구에서 감소하였으며, 적색도는 microwave 1 min 처리구는 감소한 반면, 나머지 처리구는 증가하였다. 황색도는 autoclave 50 min 처리구와 autoclave 50 min/microwave 1 min 병행 처리구에서 증가한 반면, 나머지 물리적 처리구에서는 감소하였다. 삶은 면의 색도에서는 명도, 적색도, 황색도 모두에서 무처리구에 비하여 물리적 처리구에서 증가한 값을 나타내었다. 물성 측정 결과는 경도, 부착성, 응집성, 검성, 복원성에서 무처리구와 비교시 microwave 1 min 처리구는 유의적으로 감소하였으며, 그 외 처리구에서는 유의적으로 증가한 값을 나타내었다. 탄력성과 전단력은 모든 처리구에서 유의적으로 증가하였으며, 인장력은 가압가열 30 min 처리구와 무처리구가 유의적인 차이를 보이지 않았으나, 나머지 물리적 처리구에서는 유의적으로 증가하였다. 관능 평가 결과는 생면의 경우 색 항목에서 물리적 처리구들이 유의적으로 낮은 점수를 받았다. 맛, 질감, 향 항목에서는 무처리구와 물리적 처리구간 유의적 차이를 보이지 않았다. 전체적인 기호도에서는 microwave 1 min 처리구가 가장 높은 점수를 얻었으며, 가압가열(50 min)과 microwave(1 min) 병행 처리구는 무처리구와 비슷한 점수를 얻었다. 따라서 제면 후 가압가열 및 microwave 처리가 생면 및 삶은 면에 있어서 색과 질감 항목을 보완, 개선시킨다면 알러겐성이 저감화된 면을 제품화하는데 적합할 것으로 사료되어진다. This study was conducted to determine the effects of physical treatments for quality of wet noodles. Noodles were being tried with a microwave (for 1 min), an autoclave (for 30 or 50 min), and both autoclave and microwave (for 30/1 min or 50/1 min). The results showed that the pH levels were slightly decreased after treatments of autoclave and autoclave/microwave. The moisture contents were considerably decreased as compared to the control except autoclave (50 min). After all treatments, the lightness was decreased in all samples, but, redness was increased (except microwave) and the yellowness was increased after autoclave (50 min) and autoclave/microwave (50/1 min). Texture was increased as compared to the control except microwave. In the sensory evaluation, the noodles treated with microwave, autoclave (50 min), and autoclave/ microwave (50/1 min) showed a high score in overall preference. From these results, both the autoclave and microwave methods can be applied to the wet noodles without diminishing its quality to a great extent.

물리적 처리에 의한 강력분 밀가루 Gliadin의 항원성 변화

강보경(Bo-Kyeong Kang),김꽃봉우리(Koth-Bong-Woo-Ri Kim),김민지(Min-Ji Kim),박시우(Si-Woo Bark),박원민(Won-Min Pak),김보람(Bo-Ram Kim),안나경(Na-Kyung Ahn),최연욱(Yeon-Uk Choi),최정수(Jung-Su Choi),최호덕(Ho-Duk Choi),안동현(Dong-Hyun A 한국식품영양과학회 2014 한국식품영양과학회지 Vol.43 No.4

본 연구에서는 가압가열 및 microwave 처리가 gliadin의 항원성에 미치는 영향을 살펴보기 위해 강력분에 가압가열과 microwave를 단독 또는 병행으로 처리하여 Ci-ELISA, SDS-PAGE 및 immunoblotting을 실시하였다. 가압가열 처리의 경우 시간이 길어질수록 IgG와의 결합력이 감소하였으며, 특히 50분 처리구에서 약 87%로 가장 낮은 결합력을 보였다. 또한 SDS-PAGE와 immunoblotting 결과에서도 무처리구에서 강하게 보였던 gliadin band가 가압가열처리에 의해 거의 소실되고 항체와 반응하지 않았다. 가압가열 및 microwave를 병행 처리 시도 마찬가지로 gliadin의 결합력이 감소하였으며, 처리구 중 가압가열 50분, microwave 5분 처리구에서 약 93%로 가장 낮은 결합력을 보였다. 반면 microwave를 단독으로 처리한 경우에는 일부 단백질의 변화는 관찰되었으나, 항원성 감소에는 효과가 없음을 확인하여 단백질 변화가 항원성에는 큰 효과를 준 것 같지 않다. 이상의 결과를 통해 가압가열 단독 처리 및 가압가열 및 microwave의 병행처리 시 gliadin의 항원성이 감소함을 확인하였다. This study was conducted to evaluate the effects of physical treatments on the antigenicity of gliadin in strong wheat flour. Strong wheat flour was treated with an autoclave (5, 10, 30, 50 min), a microwave (1, 5, 10 min), or both (10, 30, 50 min/ 5, 10 min), followed by SDS-PAGE, immunoblotting, and Ci-ELISA using anti-gliadin IgG. The results indicated that the binding ability of IgG to gliadin in strong wheat flour slightly decreased after autoclaving or autoclaving/microwaving. In particular, the binding ability was reduced to about 87% after autoclaving for 50 min and to 89% after autoclaving/microwaving (50/5 min). In addition, gliadin bands in the 50 min autoclaved group disappeared in both SDS-PAGE and immunoblotting. On the other hand, the antigenicity of gliadin was unaffected by microwaving alone. In conclusion, the results of this study suggest that autoclaving may reduce the antigenicity of gliadin in strong wheat flour.

Variation in clinical usefulness of biomarkers of acute kidney injury in young children undergoing cardiac surgery

Baek, Hee Sun,Lee, Youngok,Jang, Hea Min,Cho, Joonyong,Hyun, Myung Chul,Kim, Yeo Hyang,Hwang, Su-Kyeong,Cho, Min Hyun The Korean Pediatric Society 2020 Clinical and Experimental Pediatrics (CEP) Vol.63 No.4

Background: Acute kidney injury (AKI) is one of the most significant postoperative complications of pediatric cardiac surgery. Because serum creatinine has limitations as a diagnostic marker of AKI, new biomarkers including neutrophil gelatinase-associated lipocalin (NGAL), kidney injury molecule-1 (KIM-1), and interleukin-18 (IL-18) are being evaluated to overcome these limitations and detect AKI at an early stage after cardiac surgery. Purpose: This study aimed to investigate the clinical usefulness of these biomarkers in young children. Methods: Thirty patients with congenital heart diseases who underwent cardiac surgery using cardiopulmonary bypass (CPB) were selected, and their urine and blood samples were collected at baseline and 6, 24, and 48 hours after surgery. Serum creatinine and blood urea nitrogen levels as well as NGAL, KIM-1, and IL-18 levels in urine samples were measured, and clinical parameters were evaluated. Results: Of the 30 patients, 12 developed AKI within 48 hours after cardiac surgery. In the AKI group, 8 of 12 (66.6%) met AKI criteria after 24 hours, and urine KIM-1/creatinine (Cr) level (with adjustment of urine creatinine) peaked at 24 hours with significant difference from baseline level. Additionally, urine KIM-1/Cr level in the AKI group was significantly higher than in the non-AKI group at 6 hours. However, urine NGAL/Cr and IL-18/Cr levels showed no specific trend with time for 48 hours after cardiac surgery. Conclusion: It is suggested that urine KIM-1/Cr concentration could be considered a good biomarker for early AKI prediction after open cardiac surgery using CPB in young children with congenital heart diseases.

생체분해성 망막압정을 이용한 망막고정에 대한 실험적 연구

김용백,민병무,김창식,박근성,김승영,길숙종,조항진,이성복,노승무,송규상,강대영,조준식,양준묵,정경수,최선웅,이진호,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

Biodegradable retinal fixation devices obtain mechnical fixation of the retina with desirable chorioretinal scarring and with the potential for local, sustained release of antimetabolites and steroids to inhibit proliferative vitreoretinopathy. We manufactured a biodegradable retinal tack with barb that was designed in order to prevent intrusion from implantation of retinal tacks. This study was carried to evaluate the efficacy for retinal fixation and the capability for sustained release of drugs with a newly designed biodegradable retinal tack Biodegradable retinal tacks were made of polymers of glycolic acids and were designed with barbs in a shape to prevent the disinsertion. Biodegradale retinal tacks are divided into 3 parts, a conical portion that is inserted into the sclera, a cylinder portion that remains in the vitreous, and a neck portion between the pin and the cylinder. The tapered conical end was manufactured to allow easy insertion through the retina and choroid into the sclera. A cylinder portion was manufactured with a tapered angle that fixes firmly into the orifice of 19 gauge spinal needle. A neck portion, 0.4 mm in diameter, was designed to prevent disinsertion from following implantation of retinal tack. The applicator was a 19 gauge spinal needle and its orifice was prepared to 15°angle to accept the tapered cylinder portion of the retinal tack. The retinal tacks, secured in the needles, were passed through the formed vitreous and inserted into the retina, choroid, and sclera and were released by pushing the internal needle, usually within 2-3mm of the medullary ray of the posterior rabbit retina A retinal tack was placed in each of 8 pigmented rabbit eyes. Slit-lamp biomicroscopy, indirect ophthalmoscopy and fundus photography were performed periodically from 1 day to 8 weeks after surgery. Eight eyes were enucleated and studied by light microscopy at 8 weeks. Biomicroscopic evaluation of the animals revealed edemas adjacent to the retinal surfaces immediately after insertion of the biodegradable retinal tacks in all the animals. These edemas disappeared after 1 week. The first noticeable change in the size of retinal tacks was shown after 2weeks. The size of the retinal tacks gradually got smaller, decreasing to about one-half at 4 weeks and about one-third at 8 weeks. All retinal tacks remained in inserted places without any movement for an 8 week period. On light microscopy, epiretinal proliferations were seen to extend into the vitreous cavity. Cellular capsules that lined the inner aspect of the scleral defect caused by tack insertion were found. However the adjacent retina had a normal cytologic appearance and architecture in all specimens. We manufactured a biodegradable retinal tack that is designed to prevent intrusion from implantation of retinal tacks. All biodegradable retinal tacks reduce in size with time, but no retinal tacks extruded from the inserted place. The newly designed biodegradable retinal tack can be used for retinal fixation and may be used as a vehicle for the introduction of pharmacologic agents to prevent the cellular events that promote proliferative vitreoretinopathy.

소아 결핵성 뇌막염에 대한 임상적 고찰

김영창,민용식,박준수,정경식,박경배 순천향의학연구소 1998 Journal of Soonchunhyang Medical Science Vol.4 No.1

A total of 43 cases of tuberculous meningitis in children who were admitted to the pediatric department of Soonchunhyang Chunan hospital and Soonchunhyang Gumi hospital from Jan. 1983 to Dec. 1997 were reviewed clinically. The results were as follows; 1) The incidence of tuberculous meningitis admitted patients has been decreased. 2) The ratio of male to female was 1.5:1. 3) The incidence by age was highest under the age of 1 year (32.6%) and 72.1% of the patient were under 6 years. 4) There were tuberculous patients in the family in 34.9% of all cases. 5) The symptoms on admission were, in order: vomiting, fever, headache, irritability, impaired consciousness, lethargy and convulsion. 6) In the neurological examination on admission, 41.8% of all cases were in a coma or a semicomatose state; Kernig or Brudzinski sign was positive in 51.2% of the cases. 7) The tuberculin skin test was positive in only 14.2% of the cases. 8) On chest X-rays, tuberculous lesion was seen in 32.6%, and miliary patterns were seen in 9.1% of all cases. 9) Brain CT was performed in 8 cases and brain MRI in 1 case, with abnormal findings in 100% of these cases. The brain CT findings were hydrocephalus in 8 cases; basal cisteral enhancement in 2 cases; and infarction and tuberculoma in one case each. 10) In cerebrospinal fluid study, the mean leukocyte count was 174.3/μL (51 to 200/μL). The mean protein level was 154.9 mg/dL. The mean sugar level was 38.7 mg/dL. The mean chloride level was 110.8 mEq/L.. 11) The overall mortality rate was 11.9% and all patients classified as the 3rd stage on admission died.

생체분해성 고분자를 이용한 사시수술용 제형개발 및 효용성에 대한 연구

민병무,김용백,김승영,김창식,박근성,길숙종,조항진,이성복,노승무,송규상,강대영,조준식,양준묵,정경수,최선웅,이진호,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

A new device, muscle clamping system was developed to facilitate exact quatifying technique A strabismus surgery, and reduce the risk of complications. The device is composed of a lower fixing body with three jaws and an upper supporting body. They are used to clip an extraocular muscle and fix it to the sclera with a single bite. Superior rectus recession on 16 rabbit eyes were performed with this new device. Conjunctival injection, muscle adhesion strength, and light microscopic findings were examined at 1, 2, 4 and 8 weeks postoperatively. The Conjunctival injection were minimal, adhesion power ranged from 420 to 600 gram gravity, which is sufficient in withstand the normal pull of human extraocular muscle. In microscopic exam, some Inflammatory cells and fibrosis were found. The new device was technically easy, fast, and accurate, so it may be useful in stabismus surgery.

12Cr 강의 화염경화 표면처리 층의 초음속 물방울 충격에 의한 손상 거동

김광호,이민구,김경호,김흥회,김길무 대한금속재료학회 2005 대한금속·재료학회지 Vol.43 No.1

Water drop impact erosion properties of the 12Cr steel, currently used as nuclear steam turbine blade material, surface-hardened by the flame hardening process have been characterized. For this, variations of both the maximum erosion depth de,,,,ax and volume loss V with the cumulative impacts n at the supersonic impact velocity (~350 m/s) have been investigated for the as-received and flame-hardened 12Cr steels with different hardnesses. Typically all the samples showed an erosion-time characteristic involving the incubation period initially followed by the steady state period. It is also found that the d_(e,max) increases stepwise-like with the number of impacts n, which results from a sudden formation of craters by crack propagation. Compared to those for the as-received 12Cr steel, the flame-hardened ones showed an excellent resistance to water drop impact erosion with 2.2~2.8 times higher incubation time n, and 115~1/8 times lower erosion rate a. In the incubation period the as-received 12Cr steel was damaged by ductile depression and ploughing, while the flame-hardened 12Cr steel by fatigue cracks and brittle platelet deformation. Erosion in the steady state period was developed by the cleavage fracture commonly. (Received July 22, 2004)

남자 대학생의 음주 형태 변화에 영향을 미치는 유전적, 심리사회적 요인

김시경,이수경,김민경,이상익 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.4

Objectives : This study aimed to determine the general predictive factors of change in drinking behavior and to provide materials for preventing drinking problems during early adulthood through examining genetic and psychosocial factors affecting the change of drinldng behavior in college students. Methods : The subjects were 101 male college students, apart of 534 students who had completed the previous study in 2000. In the present study as a 6-years follow up, we reassessed the drinking pattem and psychosocial variables and compared the results with previous data of the same subjects. To identify factors affecting the current drinking pattem, we used stepwise multiple regession and logistic regression analysis. Results : D allele (ALDH2) was found to reduce the degree of drinking and suppress problematic drinking, and C allele (TPH) had a suppression effect on problematic drinking. Drinking motive had a direct effect on the degree of drinking and Pro-blematic drinking. Negative cognitive expectancy had a direct effect on problematic drinking. Conelusion : Authors found some factors affecting the change of alcohol drinking behavior in college students and confirmed that there were hierarchies of significance among these factors. These may be applicable as variables for predicting drinking behavior in early adulthood.

p-Anisaldehyde가 조골세포 분화에 미치는 영향

김경민 ․ 이도원 ․ 김현준 ․ 김아랑 ․ 장원구 대구대학교 산업기술연구소 2018 産業技術硏究 Vol.29 No.2

p-Anisaldehyde is a natural fragrance extracted from Pimpinella anisum L., and used as a preservative. This study examined the effect of p-anisaldehyde on osteoblast differentiation. First, cytotoxicity tests were carried out after examining the concentrations that did not show toxicity to the cells. The expression of osteoblast differentiation marker gene was confirmed by RT-PCR and was not effective in the treatment with p-anisaldehyde alone. However, p-anisaldehyde decreased the expression levels of inhibitor of differentiation-1 (Id1), distalless related homeobox (Dlx5), and runt-related transcription factor 2 (Runx2), which are osteogenic differentiation marker genes, which are increased in osteogenic condition. alkaline phosphatase (ALP) activity was also confirmed by ALP staining that p-anisaldehyde reduced ALP activity. These results show that p-anisaldehyde is effective in reducing osteoblast differentiation.

B_(2)O_(3)를 첨가한 Ni-Cu-Zn Ferrite의 자성에 대한 소결온도 효과

김민경,이명호,고재귀,송승기 명지대학교 자연과학연구소 2005 자연과학논문집 Vol.24 No.-

B_(2)O_(3)가 첨가된 Ni-Cu-Zn 페라이트의 소결온도 변화에 따른 자기적 성질을 연구하였다. 실험에 사용한 시료는 0.1 wt%의 B_(2)O_(3)를 (Ni_(0.204)Cu_(0.204)Zn_(0.612))Fe_(1.198)O_(2.98)에 첨가하여 각각 1000℃, 1100℃ 그리고 1200℃으로 4시간 동안 소결시켜 제작했다. 시료의 자기적 성질은 X-ray 회절 패턴, 자기이력 곡선, 초투자율, 손실계수 그리고 비저항을 측정하여 연구하였다. 자기이력 곡선과 초투자율 측정 결과로부터 세 시료 모두 우수한 연자성체가 되었음을 확인하였고, 소결온도가 높을수록 coercive force(H_(c))의 감소와 초투자율의 증가가 관찰되었다. 특히 가장 높은 온도(1200℃)로 소결한 시료의 Hc는 0.217Oe이었고, 초투자율은 전 영역의 주파수에 걸쳐 가장 큰 값을 유지하였다. 전력손실은 1 ㎑~10 ㎒ 주파수 영역의 교류에 대해 시료 모두 안정된 값을 갖고, 1200℃로 소결된 시료의 전력손실과 비저항은 시료 중 가장 적은 값을 가졌다. We have studied the dependence of B-doped Ni-Cu-Zn ferrite on sintering temperature. The samples were prepared from 0.1 wt% B_(2)O_(3)-doped (Ni_(0.204)Cu_(0.204)Zn_(0.612))Fe_(1.198)O_(2.98) ferrites and sintered at 1000℃, 1100℃ and 1200℃ for 4 hours respectively. The crystal structures of the samples were identified by X-ray diffraction pattern, and the magnetic properties of those were investigated by measuring X-ray diffraction pattern, hysteresis curve, initial permeability and resistivity. The results showed that all of the three samples were good soft magnetic materials, and initial permeability increased with the decreasing of Hc as the sintering temperature became higher. Especially, the sample sintered at 1200℃ showed Hc of 0.217Oe and maintained the highest value of initial permeability through the whole frequency range. The loss factors of the samples showed good frequency stability from 1 ㎑ to 10 ㎒ and both the loss factor and resistivity of the ssample sintered at 1200℃ was the least.