Transcriptional activation of hypoxia-inducible factor-1α by HDAC4 and HDAC5 involves differential recruitment of p300 and FIH-1

Seo, Hee-Won,Kim, Eun-Jin,Na, Hyelin,Lee, Mi-Ock Elsevier 2009 FEBS letters Vol.583 No.1

<P><B>Abstract</B></P><P>The interplay between hypoxia-inducible factor-1α (HIF-1α) and histone deacetylase (HDACs) have been well studied; however, the mechanism of cross-talk is unclear. Here, we investigated the roles of HDAC4 and HDAC5 in the regulation of HIF-1α function and its associated mechanisms. HDAC4 and HDAC5 enhanced transactivation by HIF-1α without stabilizing HIF-1α. HDAC4 and HDAC5 physically associated with HIF-1α through the inhibitory domain (ID) that is the binding site for factor inhibiting HIF-1 (FIH-1). In the presence of these HDACs, binding of HIF-1α to FIH-1 decreased, whereas binding to p300 increased. These results indicate that HDAC4 and HDAC5 increase the transactivation function of HIF-1α by promoting dissociation of HIF-1α from FIH-1 and association with p300.</P><P><B>Structured summary:</B></P><P>MINT-6802187:</P><P><I>HIF1 alpha</I> (uniprotkb:Q16665) <I>physically interacts</I> (MI:0218) with <I>FIH1</I> (uniprotkb:Q9NWT6) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-6802058:</P><P><I>HIF1 alpha</I> (uniprotkb:Q16665) <I>physically interacts</I> (MI:0218) with <I>HDAC4</I> (uniprotkb:P56524) by <I>pull down</I> (MI:0096)</P><P>MINT-6802021:</P><P><I>HIF1 alpha</I> (uniprotkb:Q61221) <I>physically interacts</I> (MI:0218) with <I>HDAC4</I> (uniprotkb:P56524) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-6802036:</P><P><I>HIF1 alpha</I> (uniprotkb:Q61221) <I>physically interacts</I> (MI:0218) with <I>HDAC5</I> (uniprotkb:Q9UQL6) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-6802102:</P><P><I>HIF1 alpha</I> (uniprotkb:Q16665) <I>physically interacts</I> (MI:0218) with <I>HDAC5</I> (uniprotkb:Q9UQL6) by <I>pull down</I> (MI:0096)</P><P>MINT-6802121, MINT-6802156:</P><P><I>P300</I> (uniprotkb:Q09472) <I>physically interacts</I> (MI:0218) with <I>HIF1 alpha</I> (uniprotkb:Q16665) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P>

혈액투석실 간호사의 비판적 사고성향과 임상의사결정능력이 환자안전역량에 미치는 영향

서미란(Mi-ran Seo),정경희(Kyung-Hee Chung) 인문사회과학기술융합학회 2018 예술인문사회융합멀티미디어논문지 Vol.8 No.8

This descriptive research is to provide preliminary data for the development of a nursing service program that ensures the safety of hemodialysis patients by identifying the effects of nurses’ critical thinking disposition and clinical decision making ability on the patient safety competence. The research participants were recruited through convenience sampling. The survey was conducted on 202 nurses who had worked for three months or longer in a hemodialysis unit. Critical thinking disposition was measured using the instrument to measure critical thinking disposition developed by Yun Jin (2004), and clinical decision making ability was measured using the Korean version of the Clinical Decision Making in Nursing Scale (Jenkins, 1985), which was translated and revised by Baek Mi-kyung (2005). The instrument to measure patient safety competence in this study is the one developed by Lee Nam-ju (2012) and then revised and supplemented by Jang Hae-na (2012) for clinical nurses. In summary, Consequently, education programs tailored for nurses according to their years of experience and position need to be developed and implemented. In addition, policies related to patient safety accidents should be prepared and continuous education should be provided for nurses working at a hemodialysis unit. 본 연구는 서술적 조사연구로 혈액투석실에 근무하는 간호사의 비판적 사고성향과 임상의사결정능력이 환자안전에 미치는 영향을 확인하여 혈액투석환자의 안전간호를 위한 서비스 프로그램 개발의 기초자료를 제공하기 위해 수행되었다. 연구대상은 혈액투석실 근무경력 3개월 이상의 간호사 202명이며, 구조화된 설문지를 이용하여 자료를 수집하였다. 연구도구는 윤진(2004)이 개발한 비판적 사고성향 측정도구, Jenkins(1985)가 개발한 The Clinical Decision Making in Nursing Scale을 백미경이(2005)이 수정한 임상의사결정능력 측정도구, 이남주(2012)가 개발한 환자안전역량 측정도구를 장해나(2013)가 수정한 도구를 사용하였다. 연구결과 혈액투석환자의 안전간호 실현을 위해 간호사의 비판적 사고성향과 임상의사결정 능력이 주요한 영향요인임을 확인하였으므로 직급별 맞춤형 교육프로그램을 개발하여 적용하고, 환자안전을 위한 정책을 마련하여 지속적으로 환자, 보호자 및 간호사에 대한 교육이 필요할 것으로 사료되었다.

수술로 호전되지 않은 췌장 농양을 내시경 치료로 완치한 1 예

서희영;전태주;김선영;강미선;허란;박지영;서동대;오태훈 인제대학교 백병원 2011 仁濟醫學 Vol.32 No.-

Necrotizing pancreatitis develops in about 10-20% of patients with acute pancreatitis, and pancreatic abscesses develop in 3% of them. The mortality of pancreatic abscess has been reported up to 40% in one study. Recently, there are several reports that show good results with n on-surgical therapy, such as percutaneous drainage and endoscopic drainage, but if the non-surgical drainage fails, surgical therapy are recommended. However, there is no definite therapeutic method which is suggested yet so far after the failure of the surgical drainage. So we report the case in which we experienced successful treatment of pancreatic abscess by endoscopic removal of necrotic tissue through the gastrocystostomy, after the failure of the surgical debridement and internal drainage through gastrocystostomy.

간접부착술식시 치면과 레진 베이스의 간격 및 접착제에 따른 결합강도

서미영,박병주,경희문,황현식 전남대학교 치의학연구소 2001 구강과학 Vol.13 No.3

The purpose of this study was to evaluate the feasibility of the combined use of paste resin with sealant as an adhesive of the indirect bonding. The resin bases made of lighcured resin were bonded to the bovine incisors at a distance of 0.0, 0.2 or 0.4mm using the sealant only or the mixture of sealant and paste resin. Bond strength of each group was measured using universal testing machine. The results were as follows: 1. The bond strength was reduced in both groups as the distance between enamel surface and resin base increased. 2. The bond strength showed no statistical difference between two groups in case the distance between enamel surface and resin base was 0.0mm. 3. In case the distance between enamel surface and resin base was 0.2 or 0.4mm, the sealant with paste resin group showed significantly higher bond strength than the sealant only group. The results of the present study indicate that the use of paste resin with sealant may be desirable as an adhesive in indirect bonding, particularly in case a gap is suspected between enamel surface and resin base.

일괄공정 압저항혈 압력센서의 특성

서희돈,이동원,손미정,최세곤 嶺南大學校 工業技術硏究所 1998 工業技術硏究所論文集 Vol.26 No.1

This paper describes structural characteristics of three kinds of piezoresistive pressure sensors, which include wheatstone-bridge type, center-boss type and single-element type, fabricated on one-chip by batch process. In order to optimize device dimensions with respect to the desired characteristics, analytical formula and finited-element modeling(FEM) are used in design process. In experiments the pressure sensitivity of wheatstone-bridge type is about 2.2㎷/V·㎪. The fabricated center-boss pressure sensor has improved in the characteristics as linearity at low pressure, and sensitivity is about 2.415㎷/V·㎪. In single-element type with a four-terminal gauge using shear stress, the maximum sensitivity about 0.4㎷/V·㎪ appeared at the center of the diaphragm with the 3:1 of the length : width ratio. this type is very appropriate method to minimize the temperature dependance of the offset voltage and sensitivity, because the fabricated sensors reduce the offset of themal stress and mismatch of piezoresistros as compared with the wheatstone-bridge type.

대법원 판례를 통해 본 의료소송 내용분석

서정희,복미정 울산대학교 2000 생활과학논문집 Vol.1 No.2

이 논문은 1953년에서 1998년 5월까지 법원공보 및 판례공보에 수록된 대법원 판례들중 의료소송에 관한 판례 78건을 내용분석 함으로써 우리 나라의 의료소송 실태를 파악하고자 한다. 연도별 의료소송 판례비율, 진료과목별 의료소송 실태 분석, 진료유형별 의료소송 실태 분석, 의료소송 유형별 실태, 의료소송의 결과 등을 구체적으로 분석함으로써 의료소송에 관한 소비자보호제도에 필요한 기초자료를 제공하고자 하였다. 1990년대에 의료소송사건이 크게 증가하였으며, 진료과목별로 분석을 하면 산부인과 사고가 가장 많았고, 진료유형별로 보면 수술에 관한 사고가 가장 많았다. 1990년대에 들어서면서 의료소비자가 승소하는 경우가 증가하기는 하였으나, 여전히 의료소비자는 의료인의 과실 및 그 과실과 손해발생 사이에 인과관계를 입증하고 증거를 제시하는 과정에서 불리한 상황이다. This paper analyzed the medical cases of the Supreme Court from 1953 to May in 1998. The purpose of this paper was to provide the basic data to enact the consumer protection system in medical malpractice claims. For this purpose the content of 78 cases were analysed in detail. The number of cases were increased in 1990. The number of medical cases were most in gynecology among clinic types and in surgical operations among treatment type. In 1990 The number of cases that the medical consumer won a suit were increased. But the medical consumers are still in disadvantageous position to prove the causal relation between the medical malpractice and the damage.

구자가 卵巢摘出로 誘發된 흰쥐의 骨多孔症에 미치는 影響 (2)

徐富一,金先熙,卞晟僖,金美麗 대한본초학회 1998 大韓本草學會誌 Vol.13 No.2

This study was performed to investigate the effects of Allii Tuberosi Semen(菲子) on osteoporosis after ovariectomy in rats. Control group received a daily po administration of normal saline for 4 weeks after oophorectomy(OVX). The other experimental group received a daily po administration of water extract of Allii Tuberosi Semen for 4 weeks after oophorectomy(OVX-ATS). Therefore, we measured levels of calcium, phosphorus, ash weights in femur. These results were as follows : 1. In the level of bone phosphorus, the result of OVX was 63.85±1.86㎎/㎗, but the result of OVX-ATS was 68.18±0.85㎎/㎗. But there was no statistical significance. 2. In the level of bone calcium, the result of OVX was 141.66±2.19㎎/㎗, but the result of OVX-ATS group was 164.82±3.74㎎/㎗. And the result of OVX-ATS showed significant increase in comparison with OVX. 3. In the level of bone ash weight, the result of OVX was 0.31±0.005(g), but the result of OVX-ATS was 0.33±0.002(g). And the result of OVX-ATS showed significant increase in comparison with OVX. The observations from these studies and the past studies(=A study on the effects of Allii Tuberosi Semen in ovariectomized osteoporosis of rats) suggest possible protective effect of Alli Tuberosi Semen water extract against bone loss in ovariectomized rats.

전신성 홍반성 루푸스에서 F-18 FDG PET상 기저핵 포도당대사 증가 소견을 보이는 무도병 1예 : Evidence for Bilateral Putaminal Hypermetabolism on F-18 FDG PET

서욱장,정선미,고수진,이창근,김재승,임주혁,유빈,문희범 대한핵의학회 2003 핵의학 분자영상 Vol.37 No.5

Purpose: We describe a 54-year-old woman with systemic lupus erythematosus (SLE) who suddenly presented with chorea and had positive antiphospholipid antibodies. F-18 FDG PET showed abnormally increased glucose metabolism in bilateral putamen and primary motor cotex. Tc-99m ECD SPECt also showed abnormally increased regional cerebral blood flow in bilateral putamen. She was treated with corticosteroid and aspirin after which the symptoms improved. Four months later, follow up F-18 FDG PET showed improvement with resolution of hypermetabolism in bilateral putamen. This case suggests that striatal hypermetabolism is associated with chorea in SLE.

소비자의 술집 평가기준 척도개발에 관한 연구

서정희,복미정,강한나 울산대학교 2005 생활과학논문집 Vol.6 No.2

이 논문은 울산광역시의 울산대학교 학생 200명을 대상으로 하여 소비자의 술집평가기준에 관한 척도를 개발하였다. 대학생 소비자가 술집을 선택할 때 중요하게 고려해야 할 평가기준으로 25개 문항을 선정하였다. 25개 문항을 요인분석 한 결과 8개의 요인이 추출되었다. 각 요인에 속하는 문항들의 기여도를 알아보기 위하여 요인에 포함된 모든 문항을 더한 점수를 종속변수로 하고 각 문항의 점수를 독립변수로 하여 단계별 회기분석을 함으로써 설명력의 증가분을 검토하였다. 마지막으로 척도의 신뢰도를 알아보기 위하여 25개 문항의 상관관계를 검토하였고, 요인별 신뢰도계수를 구하였다. 이러한 과정을 거친 결과 이 논문에서 개발한 척도는 신뢰도가 높다는 사실을 경험적으로 입증되었다. This research developed a scale to measure consumers' evaluative criteria about a drinking house. The data was collected from 200 college students in Ulsan. 25 items were selected as the criteria for the choice of drinking house. The factor analysis was used to identify the 8 sub-dimensions of consumers' evaluative criteria about the favorite drinking house. The stepwise multiple regression was used to predict the explanatory power of each items included in each factors. The Pearson's product moment correlation of inter item and reliability coefficient was calculated. This measure was found to be reliable throughout above mentioned several analyses.

읽기장애 아동의 음운처리 능력 특성 연구

김미경,서경희 한국특수교육문제연구소 2003 특수교육저널 : 이론과 실천 Vol.4 No.4

최근 읽기장애와 언어와의 관계의 중요성이 부각되면서 음운처리 결함이 읽기장애의 원인으로 널리 받아들여지고 있다. 이에 본 연구는 2학년, 4학년 일반아동 29명과 읽기장애아동 23명을 대상으로 음운인식능력 검사와 단어회상하기 검사, 문장회상하기 검사, 글자, 숫자, 사물, 색깔명명하기 검사를 통한 읽기장애아동들의 음운처리능력 특성을 연구하였다. 연구 결과 읽기장애아동들은 일반아동들에 비해 음운인식, 작업기억, 명명속도에서 유의미한 차이를 보였다. 이러한 결과를 바탕으로 일반학급과 특수학급에서 읽기장애 아동들을 지도하는 프로그램개발에 기여할 것을 제안한다. The purpose of this study was to investigate difference between normal children and children with reading disabilities, ranged from grade 2 to grade 4 in phonological processing ability. The sample was classified into two groups: (1) a group of 23 children with reading disabilities; (2) a group of 29 normal children. Students in the sample of this study do not have severe emotional problems, uncorrected vision problems, hearing loss, acquired neurological disorders. The results of this study were as follows: 1.Children with reading disabilities performed significantly poorly on test of phonological awareness than children without reading disabilities. 2.Children with reading disabilities performed significantly poorly on test of working memory than children without reading disabilities. 3.Children with reading disabilities performed significantly poorly on test of naming speed than children without reading disabilities. There is also interaction effect between the presence or absence of reading disability and grade on naming speed.