김치유래 젖산균의 균체지방산 분석을 이용한 분류학적 연구

이정숙,정민철,김우식,이근철,김홍중,박찬선,이헌주,주윤정,이근종,안종석,박완,박용하,민태익 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

표준균주를 포함한 230여개의 김치유래 젖산균에 대한 균체지방산(FAMEs)을 분석하였다. FAMEs profiles는 Euclidian Distance 17.5에 의해 7개의 Major Cluster와 1개의 Single Cluster로 나뉘어졌다. 이중 A, B, C 및 Cluster는 Leuconostoc속으로 분석되어졌고, F는 Lactobacillus속으로 분석되어졌다. 그리고 E와 G cluster는 두개의 Genus가 혼재되어 나타났으며 보충적인 연구가 필요하다. 앞으로 김치유래 젖산균의 균체지방산 분석결과를 기반으로 한 데이타베이스에 95가지 탄소원을 이용하는 수치분류학적 접근방법 및 Pyrolysis Mass Spectrometry 등의 화학적 분석 방법과 분자친화적 연구를 통한 종합적 분류정보 체계가 갖추어지면 젖산균의 신속, 정확한 동정 및 연구에 활발히 이용되어질 것이다. Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were sued for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined a Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacterial from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.

비만 개선 효과를 지닌 탄수화물 및 지방 흡수 억제 기능성 식이조성물 개발

윤유식,최선미,홍순복,홍정미,김정원,이홍석,홍성길 한국조리과학회 2002 한국식품조리과학회지 Vol.18 No.3

In a previous study, we developed a new food additive as an egg yolk antibody (IgY) against carbohydrate digestion enzymes for the regulation of blood glucose level and weight control. The IgY delayed and decreased the increment of blood glucose level after administration of sucrose in human being by 30% in 20∼30 min. We also developed a lipase inhibitor as a water extract of two kinds of herb, Platycodon grandiflorum and Solanum melongena. Twenty three volunteers were subjected to the intake of the egg yolk IgY plus the herbal extracts for 50 days. In average, the treated subjects appeared to lose 1.96 ㎏ of body weight and 3.4 ㎏ of body fat mass during the treated period. Furthermore, Panniculus adiposus and breech size were significantly decreased during the experimental period. Above results suggested that the administration of the dietary additives composed of egg yolk IgY and natural herbal extract improve the obesity by the decrement of body weight and body fat mass.

야뇨증 환아들의 심리사회적 특성에 대한 다기관 연구 : 행동 및 정서 문제를 중심으로

조수철,김재원,신민섭,황준원,한상원,박관현,이상돈,김경도,김건석,서홍진,이유식,정재용,김영균,문두건,남궁미경,한창희,조원열,김영식,배기수,이종국,정우영,신의진 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : The aim of this study was to examine the behavioral and emotional problems associated with nocturnal enuresis in Korean children. Methods : Three hundred eighteen children with nocturnal enuresis, together with their parents, completed the Child Behavior Checklist (CBCL), Disruptive Behavior Disorder Scale according to DSM-IV (DBDS), Children's Depression Inventory (CDI), State-Trait Anxiety Inventory for Children (STAIC), and Piers-Harris Children's Self-Concept Scale (PHCSC). Ninety-three normal students were selected as the control group. Results : Compared to the normal control group, the mean scores with regard to the withdrawn, social problems, attention problems, delinquent behavior, aggressive behavior, externalizing problems and total problems profiles were significantly higher in the nocturnal enuresis group according to the CBCL results. The nocturnal enuresis group also scored significantly higher in the ADHD and ODD profiles of the DBDS. The nocturnal enuresis group was more depressed and anxious than the control group according to the results of the CDI and STAI. The mean score of the PHCSC was significantly lower in the nocturnal enuresis group when compared to the normal control group. Conclusion : The results of this study suggest that children with nocturnal enuresis in Korea have clinically relevant behavioral and emotional problems. The findings support the link between nocturnal enuresis and psychopathology in Korean children.

폭풍시 사구의 침식각에 따른 해안단면의 침식에 관한 연구

이보형,표순보,이홍식,조원철 中央大學校 建設環境硏究所 1999 環境科學硏究 Vol.9 No.2

This EDUNE model developed by Kriebel was used for predicting storm-induced beach and dune erosion due to elevated water levels and waves. This model was based on the convolution integral involving a erosion-force function and an exponential erosion-response function. This numerical model adopted the equation of sediment continuity and a dynamic equation governing the cross-shore sediment transport due to a disequilibrium of wave energy dissipation levels. These equations are solved numerically by an implicit, double-sweep method to obtain the changes in position of elevation contours in the profile. In this study, the changes of a beach profile and dune erosion have been simulated for varying the dune erosion angle due to storm. The range of angle was 0.1~0.4 radian. It is shown that erosion rate is increasing according to the decrease of erosion angle.

불규칙파를 이용한 파랑에 의한 회절에 관한 연구

이홍식,최창수 中央大學校 建設環境硏究所 1994 環境科學硏究 Vol.5 No.-

This research was performed the diffraction of irregular waves due to the semi-infinite breakwater. As for the simulation of irregular waves, the directional spreading function was divided by 10, 20, and 36 respectively under the condition of a constant division M=10 of frequency spectrum. Also two kinds of parameter Smax=10 and 75 were adopted for investigating the dissipation effect in the sheltered region. As the results of comparing with division number of the directional function, some differences were found between N=10 and N=36, but no any remarkable variance were found in the comparioson between N=20 and N=36. As for Comparing with the values of Smax, the diffraction coefficient distribution for Smax=75 was lower than that of Smax=10, and also irregular diffracted wave heights were almost lower than regular waves. As the verification step, the results of diffraction wave height and period's ratio obtained by this study were compared with Goda's results, a good agreement was found between them. Therefore the method of the this study may be useful for estimating diffraction wave height and layout of breakwater.

응급의료센터 과밀화 해소를 위한 환자 전원

이경룡,구홍두,박인철,김승환,장석준,심호식 대한응급의학회 1995 대한응급의학회지 Vol.6 No.2

To reduce the degree of overcrowding in level Ⅲ emergency care centers, authors have conducted a prospective study of patient who were transferred to level Ⅰ or Ⅲemergency center based on the non-emergent patient guideline currently used at the severance Hospital, level Ⅲ emergency center, during the period of 65 days from Jan. 1st 1995 to Mar. 6th 1995. Followings are guideline for non-emergent patient described by the Korean government low: 1) systolic blood pressure greater than 80/100 of normal. 2) respiration rate between 10 to 24 per minute. 3) pulse rate between 60 to 100 per minute. 4) body temperature between 36 to 37.5℃ by rectal. 5) relatively alert mental status. 6) patient not requiring emergency operation at the time of examination. The results were as follows: 1. Among the total 5,301 patients, 464 patients ranged from age of 1 to 82 years old were transferred to level Ⅰor Ⅱ emergency center after simple treatment. 2. Among the 464 patients, 276 patients were treated in expected hospital with medical problems in 101 (36.6%) patients, and 175 (63.4%) patients had surgical problems. 3. Among the 276 patients, 122 (44.2%) patients were admitted, 145 (52.9%) patients were discharged from the emergency room after adequate treatment. 4. Among the transferred patients, simple laceration (33.0%) were most common, acute gastroenteritis (12.0%), and simple contusion (10.5%) were followed. These results suggest that about 10% of patients visiting level Ⅲ emergency care center, can be safely transferred to nearest level Ⅰor Ⅱ emergency centers on the bases of non-emergent patient guideline described by Emergency Medical Service law. These guideline, if put into proper use, can reduce the degree of overcrowding problems in level Ⅲ emergency care center through out the country.

沆瀣 洪吉周의初期 古文論 硏究 : 『峴首甲藁』를 중심으로

李弘湜 한양대학교 한국학연구소 2005 韓國學論集 Vol.39 No.-

항해 홍길주의 고문론의 실체를 총체적으로 조명하기 위한 첫 단계로, 본고에서는 고문과 관련된 핵심 논의들이 많이 수록되어 있는 『현수갑고』를 주된 텍스트로 삼아 연구를 진행하였다. 『峴首甲藁』에 수록된 고문 관련 논의들은 '도문일치에 입각한 글쓰기와 문장의 효용적 가치 추구, 다양한 전범 설정과 육경회귀, 古令의 회통과 법고창신, 의기법의 통합과 주체적 글쓰기'로 정리가 가능하다. 이상 종합된 내용들은 이후 여러 문집과 작품 창작에 있어서 중요한 규구로 작용하기 때문에, 항해의 문학을 이해하는 데 있어서 중요한 실마리를 제공할 수 있을 것이다. 가학의 전통과 연천의 영향, 풍부한 독서와 습작을 통해 확립된 항해의 고문론은 이후 연암을 접하면서 변화·확장하게 되는데, 특히 활물적 세계 인식에 기초한 활문장·활독서·생의론 등의 개념들은 항해의 문학을 더욱 생동감 있게 만들고 있다. 따라서 본 연구는 항해 고문론의 변화 양상을 밝히는 데 있어서 그 단초를 제공할 수 있을 것이다. In this research, main text is 「Hyeonsugap-go」 which has key theories for ancient documents to take a first step for illuminating the theory of Hanghae Hong, gil-ju paleography. The four major themes are : 1. writing is based on the notion that reason has to coincide with literature and pursues practical value of sentence, 2. creating various role model and returning to the Six Classics of Confucianism, 3. having conversations between ancient and modern times and compromising between old and new styles, 4. uniting the willings for writing and writing independently. These key themes can be a major clue to understanding his works since Hanghae capitalized on them to make collections of his works and create literary works. The writer established his own theory through the tradition of family study, the influence of Yeoncheon and abundant reading and constant practicing. Especially, Yonam's works allowed Hanghae's theory to be evolved and changed on the basis of the idea that All is living. For instant, notions, such as letting sentence lively, getting reading alive and having the implication clear, make his literatures vivid. So this research can be expected to lead to shed light on the phases of development of Hanghae theory.

국내 종합병원 병동부 복도공간의 환자 이용행태에 관한 비교 연구 : 중정형과 중복도형을 중심으로 Focused on the Courtyard and Double-loaded Corridor type

이홍식,김상복,양내원 한국의료복지시설학회 2005 의료·복지 건축 Vol.11 No.3

The conventional corridor space was a functional space simply linking the rooms and facilitating the circulations in a hospital. Today, however, they are no longer the spaces for simple circulation. Now, they are changing into healing spaces. Healing space must have some spatial characteristics helpful to curing the patients, and therefore, such a space should be designed to encourage patients to have diverse experiences and behaviors in terms of psychological stability, rehabilitation and personal communication. With such basic conceptions in mind, this study was aimed at surveying and analyzing patients' behaviors of using the corridor spaces for general hospital wards. To this end, middle corridors and patio-type side corridors for general hospital wards in Korea were sampled. It was found through this study that various healing behaviors shown in the conventional middle corridors were witnessed in the patio-type side corridors. On the other hand, behavioral characteristics of each area were analyzed to determine the factors encouraging patients to be cured, and thereupon, some design points for the corridor spaces of general hospital wards were suggested to help their users quick recovering.

경남지역의 토양 및 농작물중 게르마늄 함량

이성태,이영한,이홍재,조주식,허종수 한국환경농학회 2005 한국환경농학회지 Vol.24 No.1

양질의 기능성 농산물 생산을 위하여 게르마늄의 농업적 이용에 대한 기초 자료를 제공하고자 경남지역의 토양을 중심으로 지역, 지형, 토양유형 및 토성별 게르마늄 함량과 자연상태에서 재배한 농작물별 게르마늄 함량을 조사하여 그 분포특성을 검토한 결과는 다음과 같다. 토양중 게르마늄 함량을 분석할 결과 성남지역 논 토양 평균 함량은 0.24 mg/kg 이었고, 마산시, 진주시 및 하동군 지역이 0.30 mg/kg 이상으로 높았다. 지형별로는 홍적대지가 0.26 mg/kg으로 약간 높았으나 지형에 따른 큰 차이는 없었다. 토양유형별 게르마늄 함량은 보통답이 0.27 mg/kg으로 사질답과 습답에 비하여 높았으며, 토성별로는 미사질양토가 0.27 mg/kg으로 비교적 높았다. 일반농작물중 게르마늄 함량은 곡류>채소류>과일류 순으로 높았고, 쌀과 보리는 각각 약 68 및 48 μg/kg의 게르마늄을 함유하고 있었으며, 단감과 배는 각각 약 11 및 23 μg/kg으로 전반직으로 낮을 수준이었다. 채소류중 게르마늄 함량은 엽채류>근채류>과채류 순으로 높았으며 특히 상추와 열무중 게르마늄 함량이 각각 약 62 및 65 μg/kg으로서 높았다. 약용작물중 게르마늄 함량을 신선초(Angelica keiskei), 토천궁(Ligusticum chuanxiong), 인삼(Panax ginseng), 삽주(Atractylodes macrocephala), 백지(Angelica dahurica), 맥문동(Liriope platyphylla) 및 도라지(Platycodon grandiflorum)에서 100 μg/kg 이상으로 게르마늄 함량이 높았으며, 선학초(Agrimonia pilose) 및 일당귀(angelica acutiloba)의 함량은 각각 37 및 30 μg/kg으로 낮았다. To investigate the germanium content in paddy soil in Gyeongnam province, 310 paddy soil samples were collected at 19 areas in Gyeongnam, Ulsan and Busan. Contents of germanium in paddy soils were analyzed in different topographies, soil types and soil textures. Average content of germanium in Gyeongnam was 0.24 mg/kg, those of Masan, Jinju and Hadong were above 0.30 mg/kg. Germanium content with different topographies were no difference. Germanium contents in different soil types were 0.27 mg/kg in well adapted soil and 0.23 mg/kg in poorly drained soil. Germanium contents in different soil textures were 0.27 mg/kg in silt loam and 0.23 mg/kg in sandy loam. To determine germanium content on agricultural product in the field, content of germanium in cereals, vegetables and fruits were analyzed. Germanium content of agricultural product was high in the order of cereals>vegetables>fruits. In case of vegetables, germanium contents were high in the order of leaf vegetables>root vegetables>fruit vegetables. Germanium contents were high with 62 and 65 μg/kg in lettuce and young radish, respectively. To analyze the germanium content in medicinal plant, samples were collected from 19 medicinal plants at Hamyang areas. Germanium contents in Angelica keiskei, Ligusticum chuan wng, Panay ginseng and Atractylodes nacrocephala were relatively high with 100 μg/kg above.

젖소 足皮膚炎에 대한 오존수의 治療 效果

이수진,조성환,전무형,김덕환,박창식,한홍율,김명철 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

This study was carried out to determine therapeutic the effect of ozonated water therapy on bovine pododermatitis. In addition, bactericidal effect of ozonated water on etiological agent of bovine pododermatitis was examined. The pathohistological examination for the pododermatitis, according to application with ozonated water and ozone ointment was investigated. Thirty healthy cattle were divided two groups(each of 15) : control group (povidone group), treatment group (ozone solution group). Various parameters were evaluated in terms of the lameness score, swelling score, lesion score, WBC count, neutrophil count, pathohistological finding, and antimicrobial action. The decrease of lameness and lesion score were shown in hoof lesions on 14 days after application of ozonated water. Significant decrease of swelling was shown in hoof lesions on 14 days 1 Corresponding author after application of ozonated water(p < 0.01). In hematological findings, WBC count revealed values within normal range. The number of neutrophils was slightly higher than that of normal, however, this was improved on 14 days after application of ozonated water. In pathohistological findings, recovery was rapid macroscopically and microscopically in the treatment with ozonated water on the hoof lesions and ozonated water was effective. In antimicobial action, bactericidal effect was observed in treatment with ozonated water on the hoof lesions and ozonated water was effective.