Ipriflavone의 흰쥐 뼈 결손부 재생 효과

김현진(HJ Kim),김옥준(OJ Kim),김대윤(DY Kim),정지연(JY Jung),김민석(MS Kim),김원재(WJ Kim),최홍란(HR Choi),이은주(EJ Lee),김선헌(SH Kim) 대한해부학회 2003 Anatomy & Cell Biology Vol.36 No.2

Ipriflavone (isopropoxyisoflavone)은 soy isoflavone daidzein으로부터 합성된 isoflavone 일종으로 osteoporosis나 osteopenia 등에 이용되고 있다. 그러나 ipriflavone이 일차적으로 뼈 흡수를 억제함으로써 뼈 질환 치료에 임상적 효과를 갖는지 또는 뼈 형성을 촉진하는지에 대하여는 확실하지 않다. 또한 임상적 효능에 대한 효과가 대부분 골다공증의 치료 등에 국한되어 있는 반면, 단백 수준의 뼈 형성 인자의 유도 효과나 정상 뼈의 재생 효과를 밝히는 연구는 드물다. 본 연구는 in vitro에서 ipriflavone에 의한 뼈형성인자 발현에 대한 영향을 구명하고, 이를 생체에 국소적으로 직접 적용함으로써 ipriflavone의 뼈 재생 효과를 구명하고자 시도되었다. 시험관내 실험 : Ipriflavone 처리 3일 후 MC3T3-E1 뼈모세포주의 세포증식 효과는 10-8 M에서 10-4 M까지 대조군과 유 의한 차이가 없었다. 뼈 형성 지표로 알려진 collagen Iα1(I) 유전자 발현은 10-8 M에서 10-5 M까지 모든 농도에서 증가하였고 10-6 M에서 가장 많이 발현되었다. 그러나 10-4 M과 같이 높은 농도에서는 다시 크게 감소하여 대조군과 큰 차이를 보이지 않았다. alkaline phosphatase 발현은 대조군과 vehicle군과 같이 낮은 농도에서 큰 증가를 보이지 않았으나 10-5 M 농도에서 크게 증가하였다. 생체 실험 : Ipriflavone 투여 3일군에서 뼈 결손부는 대부분 혈병으로 채워져 있었고 혈병내에는 많은 혈액세포가 산재 하였으며, 실험군에서 대조군에 비하여 섬유원이 풍부하게 존재하였다. 약제 투여 7일 후 대조군과 실험군에서 모두 뼈 결손부 중심은 부분적으로 신생뼈에 의하여 재생되는 소견을 보였으며 대조군의 신생뼈는 풋뼈조직으로 구성되어 있었으 나, 실험군의 신생뼈는 일차해면뼈 조직으로 구성되어 있었다. 약제 투여 14일군에서 뼈 결손부가 뼈잔기둥 형태의 신생 뼈에 의하여 대치되어 있었으며 대조군에 비하여 실험군에서 더 많은 뼈조직으로 재생되어 있었다. 상기 연구 결과는 ipriflavone이 적정 농도에서 뼈 형성에 기여하며 뼈 결손부 재생 치유에 유용하게 사용될 수 있음을 시사한다. Ipriflavone (isopropoxyisoflavone) is a synthetic derivative from soy isoflavone daidzein. There are compelling evidences that it has an inhibitory effect on bone resorption in vitro and in vivo. It is not clarified however, whether its anti-osteopenic effect derives from inhibition of bone resorption or stimulation of bone formation. The present study aims to elucidate effects of ipriflavone on osteoblasts gene expression and possible clinical application on bone regeneration. Three days’ continuous treatment of ipriflavone had no effects on MC3T3-E1 osteoblastic cell proliferation at 10-8M to 10-4 M concentration from MTS assay. Gene expression of collagen α1(I), which is a bone formation marker, was markedly increased by ipriflavone treatment at 10-9 M to 10-5 M, but decreased to control level at 10-6 M concentration. Alkaline phosphatase, another bone formation marker, was also increased at its gene expression at 10-5 M concentration. Artificial bone defects on the rat calvaria were healed with hematoma and much more profuse and delicate fibrin at 3 days treatment of ipriflavone. Primary spongy bone was noted in bony defect center after 7 days treatment of ipriflavone, in contrast to woven bone in control group. Newly formed trabecular bone could be attained by the treatment after 14 days. These results suggested that ipriflavone can affect on osteoblasts at molecular level, and be clinically applied for local bone regeneration.

임신부 빈혈치료에 있어서 Iron Protein Succinylate (Hemo-Q) 의 임상적 효과

김장흡,김수평,김진홍,김휘준,김찬주,박미숙 대한산부인과학회 1994 Obstetrics & Gynecology Science Vol.37 No.5

Iron protein succinylate (Hemo-Q) is a new iron preparation for oral administration. Clinical efficacy and safety of iron protein succinylate were evaluated in 40 pregnancy and postpartum women under serum hemoglobulin level 10 gm/dl. The aim of this study was to assess the efficacy and tolerability of iron protein succinylate in iron deficiency anemia of pregnant and postpartum women. Most women received 2 bottles (30ml) every day for 60 days. Improvement was measured by hematologic variables and clinical symptom or sign after 30 days and 60 days respectively. In Conclusion 1. Efficacy of iron protein succinylate showed significant difference among pretreatment, 30 and 60 days treatment group in serum hemoglobulin and iron level. 2. Improvement of clinical symptom (polypnea, asthenia, anorexia) and sign (cutaneous, mucous pallor) of iron deficiency anemia were noted in both 30 and 60 days treatment. 3. Side effects (vomiting, constipation, diarrhea, gastric discomfort) were reported with 1(2.5%) to 2(5%) of 40 women.

[P2-72] Determination of Diacetyl used in Processed Corn Products for Popcorn by GC-MS

Kim MS,Hong KH,Moon GI,Bahn KN,Kwon YK,Lim HS,Chang SY,Choi JD,Park HK,Kim MC 한국식품영양과학회 2008 한국식품영양과학회 학술대회발표집 Vol.2008 No.10

ESTIMATION OF HETEROSIS BY ISOZYME AND RAPD VARIATION IN MELON (Cucumis melo L.)

Kim MS(김문수),Kim YK(김용권),Chung HD(정희돈),Chong UH(정웅희),Kim JH(김정희),Lim YP(임용표) 한국원예학회 1997 한국원예학회 학술발표요지 Vol.15 No.2

전치 태반에 관한 임상적 고찰

김문신(MS Kim),강준호(JH Kang),김학성(hS Kim),김두상(DS Kim) 대한산부인과학회 1977 Obstetrics & Gynecology Science Vol.20 No.5

1972년 1월부터 1975년 6월까지 42개월간에 중앙의대부속 한강성심병원 산부인과에 입원분만된 3,310예중예 76예의 전치태반(발생빈도 44:1)을 경험하여 다음과 같이 결론한다. 1. 전치태반의 발생빈도는 기타 발표처럼 초산부보다 경산부에 많았고 특히 연령이 증가될수록 고율화되어 갔다. 2. 전치태반의 발생 바로전의 임신의 처리를 조사한바 소파 및 기타 자궁내손상력이 61. 9% 있었는바 이러한 조작이 자궁강내에 해부조직학적 변화를 초래하여 저자의 전치태반 고율발생의 원인이 된 것으로 추측된다. 3. 전치태반의 종류를 전 전치태반 27예 부분전치태반 36예, 하방부착 13예였다. 이중 12예는 유착태반이 합병되어 있었다. 4. 전치태반때의 출혈은 대량출혈 57예, 중등량 4예, 소량 14예, 무출혈 1예 였으며, 출혈의 시작은 임신 35주 이하 51예, 36주 이상 25예였다. 5. 출혈시 동통예가 49예(61.5%), 규칙적 및 불규칙적 동통을 호소한 예가 24예(31.6%) 였다. 6. 태위이상은 임신 39주 이상 28예중 10예(35.7%) 38주 미만 48예중 40예(83.4%)였다. 7. 임상병리검사의 결과는 분만전 헤모글로빈치가 10gm/dl미만 37예, 그 이상 39예였 으며, 체온은 36°9`이하 54예, 37°- 37°9` 가 22예였다. 8. 전치태반 진단은 임상증상과 태반촉지에의한 것 58예, 임상증상과 태반촉지 및 태반 조영술에 의한 것 7예, 임상증상과 태반조영술에 의한 것 5예, 기타질환의 진단하에 제왕절개술중 발견된 것 3예, 질식분만후 용수태반박리시에 진단된 것 3예였다. 9. 분만방식은 하횡절개 27예(35.5%), 종절개 22예(29%), 제왕자궁전전술 11예(14.5%), 질식분만 16예(21%)였다. 10. 전치태반 치료에 있어서 파막의 효과를 분석한바 이를 실시한 13예중 출혈억제효과는 전예에서 발휘되었으며 이중 10예는 질식분만을, 3예는 제왕절개술에 의했다. 질식분만 10예중 2예에 있어서는 초임부 였다. 11. 주산기 사망율은 태아체중 2500gm 이상일 때에 11.4%, 임신 36주 이상일 때에 11. 9%로서 각각 그 이하군보다 월등한 저율이었고, 전전치태반일 때는 기타 종류보다 3배 이상의 고율이었다. 12. 전치태반에 대한 대기요법은 단 1예에 대하여 성공, 실시되었으나 저자의 전술한 결과로 보아 가능하면 임신 36주까지 입원, 대기함이 현명함을 알았다. 76 cases of placenta previa out of 3,310 deliveries were experienced during the period of 42 months from January, 1972 to june, 1975 in the Dept. of Obst. and Gynec. Han Gang Sung Sim Hospital, Chung-Ang University with the following results; 1. The incidence of placenta previa was more frequent in multiparas than in primiparas as in the other reports increasing highly as the age advances. 2. Evaluation of the management for the preceding pregnancy revealed that the history of curettage and the other injurious processes to the endometrial surface, was available in 61.9%. These processes were supposed to incur anatomical changes to the endometral surface, accounting for the high incidence of placenta previa in the author`s and cases. 3. The varieties of placenta previa incluede 27 cases of totales, 36 cases of the partialis and 13 cases of low-lying placenta. In 12 cases of these, planta previa in the author`s and cases. 4. The amount of hemorrhage in placenta previa was massive in 57 cases, moderate in 4 cases, scanty in 14 cases, and none in 1 case respectively. 51 cases among these took the onset of hemorrhage before 35 weeks of gestation, 25 cases after 36 weeks. 5. The hemorrhage accompanied no pain in 49 cases(64.5%), but regular or irregular pain in 24 cases(31.6%). 6. Abnormal presentation were noted in 10 of 28 cases over 39 weeks of gestation and in 40 of 48 cases below 38 weeks of gestation. 7. The value of hemoglobin checked antepartum, was less than 10 gm/dl in 39 cases. Body temperature was lower than 36°9` in 54 cases, 37 to 37°9` in 22 cases. 8. Placenta previa was diagnosed by clinical symptoms and palpation of placenta in 58 cases, 7 cases by clinical symptom, placental palpation and placentography. 5 cases by clinical symptom and placentography, 3 cases during operation for abruptio placenta, premature rupture of membrane and C.P.D., and 3 cases in the trial of manual seperation of placenta after vaginal delivery. 9. Method of delivery included low transverse section in 27 cases (35.5%), classical seciton in 22 cases(29%), cesarean hystrectomy in 11 cases(14.5%) and vaginal delivery in 16 cases(21%). 10. Amniotomy was applied in an effort to control hemorrhage in 13 cases with successes, 10 cases within which 2 primigravidas were included, were delivered through vaginal rout, 3 by cesarean section. 11. The perinatal mortality rate was much lower in the fetuses over 2,500gm (1 1.4%) and 36 weeks of gestation(11.9%) than subgroup. The perinatal mortality rate was over 3 times higher in the total placenta previa as in the other varieties. 12. Expectant management for placenta previa was succesful only in 1 case. It was found wise to lengthem the gestational period, if possible, till 36 weeks with the patients hospitalized.

난소암 환자에 대한 복합 화학요법 ( PAC Regimen ) 의 독성에 관한 연구

김두상(DS Kim),김문신(MS Kim),김용철(YC Kim),박형무(HM Park),유명숙(MS Yoo),신호문(HM Shin),김동명(DM Kim),조수현(SH Cho),조태승(TS Cho),환윤영(YY Hwang),심재철(JC Sim) 대한산부인과학회 1984 Obstetrics & Gynecology Science Vol.27 No.2

한양의대 산부인과학교실에서 1978년 10월부터 1982년 9월까지 PAC regimen(제1일에 adriamycin 50mg/㎡, 제2일에 cis-platinum 50mg/㎡, 제3~7일에 cyclophosphamide 150mg/ ㎡)으 로 화학치료를 받은 상피성난소암환자 16예를 대상으로 그 독성을 조사 분석하여 타 보고 자들의 성적과 비교 관찰한 바 다음과 같은 결론을 얻었다. 1. 혈액학적 독성은 총 16예중 10예(62.5%)에서 발생하였으며 혈소판 감소증은 3예 (18.8%), 백 혈구감소증 6예(37.5%), 빈혈 6예(37.5%)로 나타났으며 수혈은 1예(6.3%)에 실시하였다. 2. 신장에 대한 독성은 총 16예중 7예(43.8%)에서 각각 BUN증가 4예(25%) 혈청 creatinine 증 가 3예(18.8%)로 나타났으나, 전예가 경증이었다. 3. 가장 빈발하고 고통스러운 독성은 오심 구토로 전예에서 발생하였으며 perphenazine과 diazepam으로 어느정도 극복할 수 있었다. 4. 기타 말초신경독성은 5예(31.3%), 청력장애 1예(25%), 주관적인 시력장애 3예(18/8%), 간장 독성 1예(6.3%)였고 탈모증은 전예(100%)에서 발생하였다. The toxicity of PAC regimen was evaluated in 17 patients of ovarian epithelial carcinoma at the Edpartmane of Obstet. Nad Gynecol., Hanyang University Hospiatal from October, 1978 through september 1982. The PAC regimen consists of adriamycin 50mg/㎡ on the first day, cis-platinum 50mg/㎡ on the second day, and cyclophos-phanide 150mg/㎡ on the third to seventh day. It was repeated every 4 weeks. The results were as follow: 1. The hematologic toxicity occurred in 62.5%(10/16) of the patients, including thrombocytenia 18.8%(3/16), leukipenia 37.5%(6/16), and anemia 37.5%(6/16). For only one case, transfusion was require. 2. Nephrotoxicity occurred in 43.8%(7/160), including an elevation of BUN 25.0%(4/16) and an elevation of serum creatinine 18.8%(3/16). But all were in mild degree. 3. For the symptoms of gastrointestinal tract were nausea and vomiting which occurred in all cases and most distressing problems. All patients, however, were well tolerated with perphenazine and diazepam. 4. Peripheral neurotoxicity occurred in 31.3%(5/16), ototoxicity in 25.0%(1/4), blurred vision in 18.8%(3/16), hepatotoxicity in 6.3%(1/16) and alopecia in 100%.

단순균형전좌 46,XY, t(13p;17p) 태아의 산전진단 1 예

김세광(SK Kim),김인규(IK Kim),김미순(MS Kim),이명선(MS Lee) 대한산부인과학회 1989 Obstetrics & Gynecology Science Vol.32 No.11

분만력이 매우 불량하였던 산모에서 단순균형전좌 46,XY, t(13p;17p) 보인자임을 확인한 후 적절한 유전상담을 시행하였다. 양수천자에 의해 태아에서도 동일한 단순균형전좌가 잇음을 산전진단하엿으며 임신 40주에 정상 표현형의 영아를 분만시켰기에 간단히 문헌고찰과 함께 보고하는 바이다. A 27-year-old woman wa referred for genetic counseling because she had experinced three miscarriages and one live-born multiple anomalous baby. We karyotyped both partners. The mother was found to be a carrier of a simple balanced translocation . the formal karyotpye wa 46,XY, t(13p;17p) . Amniocentesis was performed at the 17th week of gestation . The fetus also was found to be a carrier of the identical translocation . A phenotypically normal male infant was delivery at the 40th week of gestation.

Brain abnormalities in Sjogren syndrome with recurrent CNS manifestations: association with neuromyelitis optica

Min, JH,Kim, HJ,Kim, BJ,Lee, KW,Sunwoo, IN,Kim, SM,Kim, BJ,Kim, SH,Park, MS,Waters, P,Vincent, A,Sung, JJ,Lee, KH SAGE Publications 2009 Multiple sclerosis journal: clinical and laborator Vol.15 No.9

<P><B>Background and objectives</B></P><P>Optic neuritis or longitudinally extensive myelitis in Sjogren syndrome (SS) suggests a neuromyelitis optica spectrum disorder (NMOSD). However, brain abnormalities of SS remain to be elucidated for the association with neuromyelitis optica (NMO).</P><P><B>Methods</B></P><P>Twelve primary SS patients (all women, 42?±?13.2?years) who had recurrent central nervous system (CNS) manifestations with brain involvement were retrospectively identified. Brain MRI, and neurologic and serologic findings were analyzed with the measurement of anti-aquaporin-4 antibody (AQP4-Ab).</P><P><B>Results</B></P><P>All patients showed brain lesions characteristic of NMO as follows: 1) the involved sites adjacent to the third and fourth ventricles and in the posterior limb of the internal capsule, 2) unique configurations, such as the longitudinal course from the internal capsule to the midbrain, large cerebral or cerebellar lesions over 3?cm, and cavity-like formations. AQP4-Ab was positive in six of eight patients tested, and all the seropositive patients showed lesions with increased diffusion, suggestive of vasogenic edema. Four patients met the revised criteria of NMO, and nine had features of NMOSDs. Of the remaining three patients showing only brain involvement, one had AQP4-Ab.</P><P><B>Conclusions</B></P><P>This study demonstrates that SS patients with recurrent CNS involvement have brain abnormalities characteristic of NMO and AQP4-Ab in Korea. The presence of AQP4-Ab in one SS patient with only brain involvement may suggest that the coexistence of NMO should be explored in SS patients with recurrent CNS manifestations, even without optic neuritis or myelitis.</P>

導入 機器의 效率的 活用을 위한 敎授-學習 敎材의 開發 : 8 個 學科 共同 硏究

李昌甲,孟琦錫,李載元,朴萬植,廷吉煥,金鍾悟,朴景采,李英浩,李東周,邕章祐,柳盛淵,趙澤東,姜理錫,李殷雄,李興浩,金健中,韓相玉,金良模,張錫明,朴俊灝,洪鳳植,金太均,金再韓,宋海永,盧載星 충남대학교 공업교육연구소 1982 論文集 Vol.5 No.1

In this study, through the combined effort and collaboration of 8 engineering education departments, teaching-learning processes and models were reviewed, and printed and bound student manuals developed on 120 different tasks and subjects. Audio-visual supportive material, already in use in laboratories, was added to form a complete teaching-learning package. In addition to promoting the maximum use of the newly acquired equipment, this study will also contribute to the development of laboratory practice, the in-service training of technical high schools teachers and help in the development of their own instructional program.

우리나라 대장용종의 임상 특성 -대한장연구학회 다기관 공동연구-

한동수 ( Han DS ),양석균 ( Yang S-K ),김원호 ( Kim WH ),김진오 ( Kim JO ),김진호 ( Kim J-H ),김태일 ( Kim TI ),김효종 ( Kim HJ ),명승재 ( Myung S-J ),박영숙 ( Park YS ),은창수 ( Eun CS ),이문성 ( Lee MS ),이상혁 ( Lee SH ),정문관 ( Jung MK 대한소화기학회 2002 대한소화기학회 추계학술대회 Vol.2002 No.-