Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술발표논문집 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

Goretex를 이용한 융비술

최진영,민철기,명훈,황순정,서병무,이종호,정필순,김명진 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.4

융비술시 실리콘 고무는 다루기 쉽고 조직 반응이 적어 현대 많이 돌출의 합병증이 생길 수 있다. 저자등은 이러한 실리콘의 단점을 보완할 수 있는 goretex를 사용하여 융비술을 시행하고 비첨에 대해서 자가연골을 이식하여 만족할 만한 결과를 얻었기에 goretex가 안면부 연조직의 증강을 위한 이식재료로 적합하다고 생각되어 문헌고찰과 함께 보고하는 바이다. Many different materials have been used to provide augmentation of the nasal dorsum. this include both autogenous and homogenous materials,as well as allografts. Silicone among the artificial materials has been most widely used. The silicone has some advantages and disadvantages. Goretex(expanded polytetra-fluoroethylene) which has widely used in vascular surgery and abdominal surgery can be used for facial soft tissue augmentation. The author presemts 2 cases of nasal augmentaion using goretex with literature review.

Value-at-Risk의 사후검증에 관한 연구 : KOSPI 200 선물지수를 중심으로

명건식,임윤수,최종환 충남대학교 경상대학부설 경영경제연구소 1999 경영논집 Vol.15 No.1

최근 금융의 자유화는 금융자산의 시장변동성을 심화시키며 대형금융사고를 야기 시키고 있다. 이에 규제기관 및 시장참여자들은 주가, 금리, 환율, 상품가격 등 시장 경제 변수의 변동위험 즉, 시장위험(market risk)관리의 중요성을 재차 인식하게 되었다. 본 논문은 금융자산의 시장위험을 측정, 관리할 수 있는 내부모델로서 KOSPI 200 선물지수를 대상으로 역사적 시뮬레이션 접근법(historical simulations), 분산-공분산 접근법(variance-covariance method), 몬테카를로 접근법(Monte Carlo simulations)을 통한 VaR의 계산과 실제거래위험과의 차이를 비교, 검토함으로써 위험관리 방법으로서의 사후적 효과성을 검증하고자 하였다. 이를 위해 1996년 5월 3일부터 1998년 9월 10일까지의 KOSPI 200 선물지수의 최근월물 만기 3개월 일별 종가를 대상으로 보유기간은 1일, 신뢰수준은 95%와 99% 그리고 Position은 전일 종가를 기준으로 매수와 매도를 가정하여 손익을 계산하였다. 본 연구결과 시장위험의 관리수단인 VaR인 KOSPI 200 선물지수의 위험관리수단으로서 95%와 99%의 신뢰수준 하에서 상기의 방법으로 측정한 결과 BIS에서 제시하는 모델의 정확성 영역구분에서 황색 또는 적색영역에 해당되는 결과를 보이고 있다. 이는 위험관리 수단으로서 VaR기법의 남용은 이에 관한 통일적 방법이나 기준이 제시되고 있지 않은 국내의 상황에서 정확성이 검증되지 않은 방법으로 시장위험을 관리하는 즉, 또 다른 위험을 야기할 수 있는 가능성을 내포하고 있음을 암시하고 있다.

고관절 외전근의 원심성 운동이 보행시 균형에 미치는 효과

최원호,김명종 대한정형물리치료학회 2003 대한정형도수물리치료학회지 Vol.9 No.2

The purpose of this study was to identify the effects of eccentric exercise of affected help abductor muscles on Gait balance in patient with hemiplegia. 30 patients with hemiplegia were recruited among in and out patients of Gil Hospital(age x=50.5, SD=10.1, range 37-80. post stroke days x=383.2, SD=309.3, range 57-1165). These patients were divided into eccentric and control groups by random sampling. TUG were used for this study. Paired t- test was used to identify the effects of eccentric exercise of hip. The results were stastically significantly differences in the effect of exercise between experimental and control groups by TUG(p<0.05). The results indicate that the eccentric exercise of affected hip abductor muscles is effects on gait balance in patient with hemiplegia.

코로나 플라즈마 처리에 의한 HDPE 표면의 개질에 관한 연구

최진숙,전종탁,정봉규,이근대,문명준,이종무 釜慶大學校 2001 釜慶大學校 論文集 Vol.6 No.-

We are able to generate the stable plasma in open air and nitrogen using a corona plasma treatment system(CPTS) with a high voltage. This was applied to treat the surface of rather non-polar HDPE(High Density Polyethylene) intended for the improvement of adhesion of over-coated layers such as coatings, printing inks. The surface tension of HDPE was significantly improved by the CPTS. The characteristics of surface layer were evaluated by means of contact angle measurement, ATR-FT/IR spectroscopy, and it was found that functional groups were introduced into the surface layer of the HDPE. The use of nitrogen was increased the surface tension of the HDPE from 33.8 mJ/m^2 to 66.56 mJ/m^2, and highly effective method of HDPE surface modification could be realized.

復合材料 連結 部位를 갖는 엔진 마운트 設計에 關한 硏究

최원종,김권진,오인택,임동준,홍영길,황명신 한국항공대학교 1993 論文集 Vol.31 No.-

본 논문에서는 초경량 항공기의 엔진 마운트 구조물에 대한 유한요소 해석을 수행하였으며, 구조의 안전성 확보에 중요한 영향을 주는 부분인 복합재료 동체와 엔진 마운트 간의 연결 부위에 대한 해석을 위해 유한요소 프로그램을 작성하였다. 본 연구에 의해 작성된 유한요소 프로그램의 신뢰성을 확인하기 위해 실제로 시편 제작 실험을 실시하여 실험치와 해석치를 비교 검토하였다. The finite element analysis was performed on the ULM's engine mount to verify the structural safety Result showed that proposed engine mount structure has a large margine of safety. Strains and stresses in the vicinity of the joint between a composite fuselage and a metallic engine mount were calculated by the finite element analysis program. Numerical results from the model were compared with the test results. Good agreement was found between experiment and model results.

하악골 골절후의 골치유과정에 있어서 방사선학적 추적조사

최진영,김명호,허경희,서병무,이종호,정필훈,김명진 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.5

The results of radiographic study of healing of fractured mandible are often inconsistent with clinical evaluation of bone healing pattern of it. In addition, there is inconsistency between radiographic studies. We think if such inconsistency is resolved, clinical assessment combined with radiologic evidence can be used to guide to more accurate clinical management. The purpose of this study is to evaluate radiological healing pattern of mandible, and to apply its result to clinical use. Panoramic view was used to analyze the change of radiographic finding in 35 patients (27 male, 8 female, ranging 17 to 53 years old) treated for mandibular fracture at the department of oral & maxillofacial surgery, SNUH in the period 1991 to 1998, Patients are all treated by rigid internal fixation using monocortical miniplate. Within 9 weeks, no change is observed at fracture site. Osteogenic change is observed at various time interval (3 to 21 weeks). We have found radiographic change of healing is observed 4 to 6 weeks later to clinical healing, According to this result, we recommend radiographic follow-up at 4 week, 10 week, 10 month.

6주간의 淸暑益氣湯 經口投與가 에너지대사 및 호르몬 변화에 미치는 영향

최용어,강명신,유종만,이규성,오재근,조준용 韓國體育大學校附屬 體育科學硏究所 1994 韓國體育大學校附屬 體育科學硏究所論文集 Vol.13 No.1

The purpose of this study was to investigate the effects of chung-seo-ik-gi -tang administration on the metabolic responses and hormones in 18 healthy-male elite boxes. All subjects were divided randomly with two groups, Chung-sea-ik-gi-tang Group(N=18) and placebo group(N=18) and perfored with 70% of HR max using bycicle ergometer unfil exhaustion. During the submaximal exercise, heart rate was maesured every two min. The blood samples were collected from antecubital vein by 10ml syranges(6ml/each) before immediately after exhaustion, recover-10 min, recover-20 min and recover-30 min. The samples were to analyzed for the level of glucose, lactate, TG, TC, HDL-C, insulin and catecholamine. The results of various blood parameters were summarized as follow; l. Energy substrates (plasma glucose, TG, TC, HDL-C) changes in group C were significantly different from group P. Plasma glucose in group C was significantly lowered at recover-10 min compared to group P (P<0.05). and also TG, TC and HDL-C in group C were significantly increased after 3 weeks compared to group P (P<0.05) whereas. Lactate accumulation in group C was significantly decreased after 3 weeks compared to group P (P<0.05). 2. Plasma insulin level was not showed significant difference between two groups. But insulin depression tendency after 3, 6 weeks in group C was allevated compared to group P. Increasing rate of norepinephrine in group C at Post-exhaustion after 3weeks lowered compared to group P. Norepinephrine in group C at rest periods after 3 weeks showed significant difference compared to group C. The results indicated that Chung-seo-ik-gi-tang had the effects of ergogenic aids on fatigue resistance and recovery from exhaustive exercise at the level of 70% HR max.

社會變遷 속의 作業態度 : 販賣職女從業員에 대한 實證的硏究

崔鍾泰,吳命根,J. Platzer 대구효성가톨릭대학교 사회과학연구소 1975 女性問題硏究 Vol.4 No.-

인공배지의 기비첨가와 시용량 결정

최종명,정병룡 경상대학교 시설원예연구소 1995 施設園藝硏究 Vol.2 No.-

원예작물의 온실 재배에 있어서 안정적인 작물 생산을 위해서는 온도, 광도, 습도, 또는 토양 조건을 최적의 상태로 조절해 주어야 한다. 특히 온실내의 작물재배에 있어서는, 과거의 노지재배와 달리 다양한 규격의 플러그트레이나 포트와 같은 용기안에 인공배양토를 채워 넣고 작물을 식재하고 있기 때문에 환경 조절에 더 많은 관심을 기울여야 한다. 인공 배양토가 이용되는 중요한 원인은 일반 노지 토양은 입자의 크기가 너무 작기 때문이다. 따라서 식물 재배시 배양토안의 O^2나 CO^2의 확산이 억제되며 결과적으로 불건전한 뿌리발달이나 식물생육을 초래하게 된다. 그러므로 입자가 큰 배양토를 적정 비율로 혼합하므로써 토양공기의 확산을 증가시키게 된다. 이러한 배양토의 물리성을 개선하기 위하여 재배농가에서는 피트모쓰, 버미클라이트, 플라이트 또는 다른 배양토의 구성물질을 적정비율로 혼합한다. 이렇게 하므로써 배양토가 적절한 수분 보유력과 공기의 확산 능력을 갖도록한 후, 적당한 양의 비료성분을 공급하여 안정적인 작물생산을 시도하고 있다. 그러나 인공 배양토는 다음과 같은 측면에서 문제점을 안고 있다. 첫째, 배양토의 양이온 치환용량 (CEC)이 부족하여 완충력 (buffering capacity)이 낮기 때문에 식물체가 pH의 변화 또는 무기원소의 결핍과 과다에 쉽게 영향을 받아 안정적인 작물생산에 장해요인이 된다. 둘째 인공배양토는 노지토양에 비해 낮은 무기비료 성분을 함유하고 있다. 이상과 같이 배양토의 화학적인 특성에 기인하는 낮은 완충력때문에 노지 재배에서와는 달리 쉽게 배양토의 pH가 변하며, 무기원소의 과다나 부족에 기인하는 독성이나 결핍증상이 유발된다. 그러므로 pH 및 모든 비료성분의 양을 정확하게 조절하여야 안정적인 작물생육이 가능해지며, 배양토의 물리 또는 화학성을 바탕으로 한 적절한 시비체계의 확립이 온실산업의 성패를 결정짓는 중요한 요인이 될 수 있다. Fertilization systems include liquid fertilization known as fertigation and preplanting fertilization. The fertigation system has problems in heavy leaching of fertilizer solution, increase of labor and amout of fertilizer used, and ground contamination. Preplanting fertilization overcomes the problems in fertigation system. However, when plant develop nutrient deficiency or toxicity symptoms, correcting fertilization is very defficult in preplant fertilization systems because greenhouse substrate has low buffering capacity. Correct adjustment of substrate pH and incorporation of right amount of fertilizers can prevent those problems. In preplant fertilization system dolomite adjust substrate pH and supply Ca and Mg where superphosphate provides for P, Ca, and S. Micronutrient mixes can supply 6 micronutrients in that system. But selection of the amount of fertilizer incorporated into substrate depend on the characteristics of substrate, fertilizer concentration, and cost.