AtCPL5, a novel Ser‐2‐specific RNA polymerase II C‐terminal domain phosphatase, positively regulates ABA and drought responses in Arabidopsis

Jin, Yong‐,Mei,Jung, Jinwook,Jeon, Hyesung,Won, So Youn,Feng, Yue,Kang, Jae‐,Sook,Lee, Sang Yeol,Cheong, Jong‐,Joo,Koiwa, Hisashi,Kim, Minkyun Blackwell Publishing Ltd 2011 The New phytologist Vol.190 No.1

<P><B>Summary</B></P><P><P>Arabidopsis RNA polymerase II (RNAPII) C‐terminal domain (CTD) phosphatases regulate stress‐responsive gene expression and plant development via the dephosphorylation of serine (Ser) residues of the CTD. Some of these phosphatases (CTD phosphatase‐like 1 (CPL1) to CPL3) negatively regulate ABA and stress responses. Here, we isolated <I>AtCPL5</I>, a cDNA encoding a protein containing two CTD phosphatase domains (CPDs).</P><P>To characterize AtCPL5, we analyzed the gene expression patterns and subcellular protein localization, investigated various phenotypes of <I>AtCPL5</I>‐overexpressors and knockout mutants involved in ABA and drought responses, performed microarray and RNA hybridization analyses using <I>AtCPL5</I>‐overexpressors, and assessed the CTD phosphatase activities of the purified AtCPL5 and each CPD of the protein.</P><P>Transcripts of the nucleus‐localized AtCPL5 were induced by ABA and drought. <I>AtCPL5</I>‐overexpressors exhibited ABA‐hypersensitive phenotypes (increased inhibition of seed germination, seedling growth, and stomatal aperture), lower transpiration rates upon dehydration, and enhanced drought tolerance, while the knockout mutants showed weak ABA hyposensitivity. <I>AtCPL5</I> overexpression changed the expression of numerous genes, including those involved in ABA‐mediated responses. In contrast to Ser‐5‐specific phosphatase activity of the negative stress response regulators, purified AtCPL5 and each CPD of the protein specifically dephosphorylated Ser‐2 in RNAPII CTD.</P><P>We conclude that AtCPL5 is a unique CPL family protein that positively regulates ABA‐mediated development and drought responses in Arabidopsis.</P></P>

Development of Competitive Direct Enzyme-linked Immunosorbent Assay for the Detection of Gentamicin Residues in the Plasma of Live Animals

Jin, Yong,Jang, Jin-Wook,Lee, Mun-Han,Han, Chang-Hoon Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.10

Competitive direct ELISA was developed to detect gentamicin residues. Mice immunized with gentamicin-keyhole limpet hemocyanin (KLH) conjugate developed good antiserum titers, which gradually increased with booster injections, indicating immunization was successfully processed. Monoclonal antibody against gentamicin was prepared using hybridoma cells cloned by limit dilution of fused cells. IgG was purified from ascites fluid of hybridoma cell-injected mice through ammonium sulfate precipitation and Sephadex G-25 gel filtration. After the gel filtration, fractions of high antibody titer were further purified through affinity chromatography on protein A/G column. Monoclonal antibody against gentamicin was confirmed as IgG1, which has kappa light chain. Cross-reactivities ($CR_{50}$) of gentamicin monoclonal antibody to other aminoglycosides (kanamycin, neomycin, and streptomycin) were less than 0.005%, indicating the monoclonal antibody was highly specific for gentamicin. Standard curve constructed through competitive direct ELISA showed measurement range (from 80 to 20% of B/$B_0$ ratio) of gentamicin was between 1 and 40 ng/ml, and 50% of B/$B_0$ ratio was about 4 ng/ml. The gentamicin concentration rapidly increased to 1,300 ng/ml after the intramuscular administration up to 2 h, then sharply decreased to less than 300 ng/ml after 4 h of withdrawal, during which the elimination half-life ($t_{1/2}$) of gentamicin in the rabbit plasma was estimated to be 1.8 h. Competitive direct ELISA method developed in this study using the prepared monoclonal antibody is highly sensitive for gentamicin, and could be useful for detecting gentamicin residues in plasma of live animals.

Design and Implementation of ETSI-Standard Reconfigurable Mobile Device for Heterogeneous Network

JIN, Yong,AHN, Chiyoung,CHOI, Seungwon,MUECK, Markus,IVANOV, Vladimir,SARKAR, Tapan K. 'Institute of Electronics, Information and Communi 2016 IEICE TRANSACTIONS ON COMMUNICATIONS - Vol.eb99 No.8

The characteristics of CO2 front dynamic migration in low permeability sandstone oil reservoirs under different miscibility degrees

Jin Yong,Wang Zhilin,Zhang Zhenxin,Lin Bo,Ge Zhengjun,You Qidong,Chen Hao,Liu Xiliang,Xu Chenghao,Gao Shuaiqiang,Wu Yi,Abro Zafar 한국자원공학회 2023 Geosystem engineering Vol.26 No.4

CO2 flooding is a significant technology for oil recovery, to reveal the microcosmic mechanism of CO2 flooding, CO2 slim tube experiments, long core CO2 flooding experiments, gas chromatography analysis experiments, and numerical simulations were carried out. The results show that the miscibility increase can improve the effect of dissolution and diffusion in the CO2-crude oil system, easing the CO2 finger entry phenomena, and delaying gas breakthrough time. Besides, the CO2 -crude oil interaction is enhanced by increasing the pressure difference between the injection well and the production well, which improves the oil recovery of CO2 flooding. Besides, CO2 carries the light and medium components (C4 ~ 25) forward to cross the first miscible zone, which makes the CO2-crude oil system form double miscible zones. This study reveals the rule of CO2 front and light components front under different miscibility degrees of CO2 flooding from the microscopic perspective and deepens the theory of CO2 flooding in low-permeability sandstone reservoirs.

사회적 불안이 해석수준과 중심부(vs. 주변부) 속성 강조 제품 만족도에 미치는 영향

고진용 ( Ko¸ Jin Yong ),여준상 ( Yeo¸ Jun Sang ) 한국소비자학회 2020 소비자학연구 Vol.31 No.6

일반적으로 불안은 주관적 감정으로 다가오는 어떤 사건에 대한 부정적 결과가 예측될 때 느끼는 구체적인 정서반응을 의미한다. 그동안 불안에 대한 연구는 주로 개인의 정서 이상 증상을 치료하기 위한 정신병리학적 접근이 주를 이뤘고 ‘불안’이 사회 속에서 소비자의 행동 변화를 예측하는 연구는 드물었다. 이 연구는 사회 속에서 개인이 느끼는 불안에 대한 개념을 우선 탐색하여, 일상적인 불안이 해석수준과 제품 속성 강조에 따라 만족도에 어떤 영향을 미칠 수 있는지를 탐구하는 것이 목표다. 실험1에서는 개인의 사회적 불안과 해석수준의 관계를 알아보았고 실험2에서는 지각된 사회적 불안이 제품 속성(중심부 vs. 주변부) 강조에 따른 만족도에 미치는 영향과 해석수준의 매개효과를 검증하였다. 실험1 결과, 측정된 개인의 사회적 불안이 높을수록 해석수준이 낮아지는 부적 관계가 확인되었다. 실험2에서는 중심부 속성을 강조한 제품 만족도에는 지각된 사회적 불안이 해석수준을 매개하는 간접효과가 유의미하였다. 주변부 속성을 강조한 경우에는 불안이 제품만족도에 직접효과를 미치는 것으로 나타났다. 본 연구의 시사점은 현대사회가 만들어 낸 불안이 해석수준과 특정 소비행동에 영향을 준다는 점을 밝힌 것이다. 따라서 이 연구는 최근의 일부 소비 트렌드가 불안, 또는 불안이 만들어 낸 낮은 해석수준의 영향이라는 설명을 제공할 수 있다. In general, anxiety refers to the specific emotional response by which a person feels when a negative outcome is predicted for an event to take place. However, researches on anxiety have mainly focused on psychopathological approaches to treat individual’s emotional disorders and there has been less interest in ‘anxiety’ as an indicator to predict consumers’ behavioral changes in the society. This research aims to figure out the effect of individual’s social anxiety on construal level and consumer preferences with the concept of anxiety that an individual feels in the society. In study 1, the relationship between individual's social anxiety and construal level was examined. In study 2, the effect of perceived social anxiety on the satisfaction for product attributes(emphasis on central attribute vs. peripheral attribute) and the mediating effect of construal level were examined. The result of study 1 shows the negative relationship between individual's social anxiety and constual level since construal level becomes lower when individual’s social anxiety is higher. Study 2 unveils that the indirect effect of peceived social anxiety on product satisfaction mediated by construal level is statistically significant for the product emphasizing on central attribute. In the case of emphasizing the peripheral attribute, anxiety shows only a direct effect on product satisfaction. Implication of this study is that anxiety in the modern society drives the low level construal which triggers the specific consumer behavior(e.g., preference for product emphasizing on peripheral attributes). So this research could provide the argument that some recent consumption trends might be caused by anxiety or lower construal level driven by precedented anxiety.

Design and Optimization of linear motor using Halbach Magnet Array with T shape magnets

Jinyong Kim(김진용),Hyunuk Kim(김현욱),Soo-Hun Lee(이수훈),Moon G. Lee(이문구) 대한기계학회 2009 대한기계학회 춘추학술대회 Vol.2009 No.11

HMA (Halbach magnet array) is the sum of vertical magnets and horizontal magnets while the conventional magnet array only has vertical magnets. Conventional studies on the linear motor with HMA have been tried to increase driving force and to reduce force ripple. In the study of Lee [1], the author suggests HMA with trapezoidal magnet array. The magnet array has high mean force and low ripple. But there are problems. First, it is very difficult to make the trapezoidal shape of magnet. And the assembly of the array is difficult. In this paper, we suggest HMA linear motor with “T” shape magnets. There are advantages with the magnet array. Firstly, compared to trapezoidal magnet, it is easy to manufacture. Second, it is easy to assemble the magnet array. Based on the SQP (Sequential Quadratic Programming) method, we propose a optimized linear motor with HMA using “T” shape magnets. The linear motor has large force and small ripple.

Mechanistic Understanding of Phototoxicity of PAHs and Metals, and Screening of Pharmaceuticals in Ecotoxicology

Jinyong Jung,Kyungho Choi 서울대학교 보건환경연구소 2006 보건학논집 Vol.43 No.2

Long-term stock price manipulation: Evidence from the Korean stock market

Jinyong Kim,Yongsik Kim 한국자료분석학회 2023 한국자료분석학회 학술대회자료집 Vol.2023 No.1

Does Design Pattern Support Easy Change Impact Analysis? - Focusing on the Command Pattern

Jinyong Park,Hyemin Noh,Cheoljung Yoo,Jihyun Lee 보안공학연구지원센터 2016 International Journal of Software Engineering and Vol.10 No.10

Design patterns, a solution for the specific context and the problem, give benefits to us what a good design creates if we adequately use them in accordance with their purposes and principles. However, if we do not use them with inadequate purposes, using design patterns can be causes of increasing costs and decreasing maintainability and system performance. An industrial robot completes its given task through a series of hardware device’s primitive movements, controlled by an application using the APIs provided by specific hardware vendors. However, required movements of a robot change frequently because the given task changes often. This requires changes on hardware devices. As a consequence of this, APIs, controlling the hardware device change, and we also should modify the corresponding application. Therefore, an industrial robot software application should be designed such that it can easily trace hardware device’s change impacts on the application as well as it defines ordered composite primitive movements for completing the required task. This paper implements parts of an application that use hardware dependent APIs by using the command pattern and validates whether it support each change impact analysis.

Why Do the Chinese Popular Protests Not Progressively Lead to Regional Uproar? : The Cause of Non-Proliferating Chinese Resistances and Concession Strategy

( Jinyong Kim ) 국방대학교 안보문제연구소 2021 The Korean Journal of Security Affairs Vol.26 No.2

The present study examines why local protests in China have not proliferated into nationwide protests, despite the rapid increase in local protests in terms of frequency, scope, and intensity. To research this phenomenon, observed only in China, this study investigates and analyzes public protest-related news articles, from the South China Morning Post from 2000 to 2016. The research reveals a three-point strategy adopted by the Chinese government to suppress protests: 1) the coercion strategy, 2) the central government’s interference that strongly urges the employment of concession, and 3) the local government’s implementation of concession prior to any interference from the central authority. This study demonstrates that proliferation of mass protests is greatly hindered by the use of armed forces along with the concession strategy, derived from economic and political interests of both central and local governments.