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Yi‑Chia Kuan,Venkatesan Thiruvengadam,Jia‑Shin Lin,Jia‑Hsin Liu,Tsan‑Jan Chen,Hsin‑Mao Wu,Wen‑Ching Wang,Liang‑Jwu Chen 한국식물생명공학회 2018 Plant biotechnology reports Vol.12 No.1
The broad-specificity amino acid racemase (Bsar) from Pseudomonas putida catalyzes the racemization of various amino acids, offering a flexible and feasible platform to develop a new non-antibiotic selectable marker system for plant transformation. In the present study, we demonstrated that a Bsar variant, Bsar-R174K, that is useful as a selectable marker gene in Arabidopsis and rice that were susceptible to l-lysine and D-alanine. The introduction of wild-type Bsar, Bsar-R174K or Bsar-R174A into E. coli lysine or asparagine auxotrophs was able to rescue the growth of these microorganisms in minimal media supplemented with selectable amino acid enantiomers. The transformation of Arabidopsis with Bsar or Bsar variants based on d-alanine selection revealed that Bsar-R174K had the greatest efficiency (2.40%), superior to kanamycin selectionbased transformation (1.10%). Whereas, l-lysine-based selection exhibited lower efficiency for Bsar-R174K (0.17%). The progenies of selected Bsar-R174K transgenic Arabidopsis revealed normal growth properties. In addition, Bsar-R174K transgenic rice was obtained on l-lysine medium with an efficiency of 0.9%, and the progenies of the transgenic rice revealed morphologically normal phenotypes comparable with their wild-type counterparts. This study presents the first report of broad range amino acid racemase Bsar-R174K as a non-antibiotic selectable marker system applied in transgenic plants.
Synthesis of mesoporous SiO2 xerogel/chitosan mixed-matrix membranes for butanol dehydration
Yi-Feng Lin,Chang-Yu Wu,Ting-Yu Liu,Kun-Yi Andrew Lin,Kuo-Lun Tung,Tsair-Wang Chung 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.57 No.-
Novel mesoporous SiO2 xerogel/chitosan (CS) mixed-matrix membranes (MMMs) were successfully prepared by incorporating sol–gel synthesized SiO2 xerogels into glutaraldehyde (GA) cross-linked CS membranes. The effects of the SiO2 doping amount and the feed temperature on the performance of the separation of butanol and water was also investigated in this study. The 0.25 wt% SiO2 xerogel/CS MMMs possessed the best pervaporation performance and the largest PSI value of 1102 kg/m2h with a permeate flux of 736 g/m2h (Permeability of 3.25 × 10−5 g m m−2 h −1 Pa−1) and a separation factor of 1498 at a feed temperature of 50 °C. This PSI value is not only comparable but also exceeds that of other membranes reported in the literature. The separation factor and the flux of the SiO2 xerogel/CS MMMs in this study clearly exceed the upper limit of the previously reported MMMs. As a result, the as-prepared novel SiO2 xerogel/CS MMMs showed an improved performance during the butanol and water separation process, which raises the possibility of future pervaporation applications using this novel SiO2 xerogel/CS MMMs.
Cross-Layer Analysis of Wireless TCP/ARQ Systems over Correlated Channels
Wu Yi,Niu Zhisheng,Zheng Junli The Korea Institute of Information and Commucation 2005 Journal of communications and networks Vol.7 No.1
In this paper, we present a cross-layer analysis of wireless TCP systems over correlated channels. The effects of error correlation on the behavior of link retransmission strategy and the end-to-end throughput of TCP layer are investigated. Based on the cross-layer analysis, an efficient refinement of link layer protocol is proposed by consciously utilizing the information of channel correlations, which leads to the performance improvement of wireless TCP systems.
Yi Wu,Xuejun Wang,성수학 한국통계학회 2019 Journal of the Korean Statistical Society Vol.48 No.3
In this paper, we mainly study the asymptotic properties of weighted estimator for the nonparametric regression model based on linearly negative quadrant dependent (LNQD, for short) errors. We obtain the rate of uniformly asymptotic normality of the weighted estimator which is nearly O(n−1/4) when the moment condition is appropriate. The results generalize the corresponding ones of Yang (2003) from NA samples to LNQD samples and improve or extend the corresponding one of Li et al. (2012) for LNQD samples. Moreover, we obtain some results on mean consistency, uniformly mean consistency, and the rate of mean consistency for the weighted estimator. Finally we carry out some simulations to verify the validity of our results.
( Yi Guang Wu ),( Zhe Jin ),( Zhong Cheng Xin ),( Wei Dong Song ),( Jing Peng ),( Zhi Chao Zhang ),( Bing Gao ),( Ze Long Kim ) 한국조직공학·재생의학회 2007 조직공학과 재생의학 Vol.4 No.4
The purpose of this experiment is to construct an in vitro model of rabbit penis enlargement using tissue engineering method, and to investigate the efficacy and safety of autologous fibroblast cell seeded to biodegradable scaffold and also to provide a new perspective of clinical application. We observed the growth of GFP transgenic fibroblast cell from SD mouse after transplantation with the biodegradable scaffold by fluorescence microscope. At the second hand, 160 male New Zealand rabbits were divided into four groups; Group A for control; Group B for acellular matrix implanted; Group C for scaffold implanted only; Group D for autologous cultured fibroblast seeded scaffold. Rabbit scrotal skin (4×4 mm) was detached under general anesthesia, and fibroblast (FB) was cultured and proliferated for 2weeks. Cultured cells were seeded into the scaffold and inserted on the between buck`s and dartos fascia of rabbit penis. To assess the efficacy and safety of the cells, we observed for 4months. The FB of rabbit were successfully cultured and confirmed by immunohistochemistry method. In the results of group D, dermal cell seeding group, the penile girth was significantly increased to 22.3% compared to other groups (Group A 0.3%, Group B 12.7%, Group C 14.6% and Group D 22.3%). We checked the results in 2~4 months later. We observed that moderate penile girth increase was gained using the biodegradable scaffold without other side-effects. Therefore, we carefully suggest a new guidance for penile augmentation by the tissue engineering method.
Structure and Properties of Konjac Glucomannan/Galactoglucomannan Nanofiber Membrane
Yi Yuan,Xin Hong,Ruojun Mu,Jingni Gong,Lin Wang,Rongxun Huang,Jiayu Wu,Yongsheng Ni,Xianhui Wu,Jie Pang 한국고분자학회 2017 Macromolecular Research Vol.25 No.10
Konjac glucomannan (KGM)/galactoglucomannan (GGM) nanofiber membranes were obtained through electrospinning technology. Rheological properties of KGM/GGM solutions were observed by using a rotary rheometer. The apparent morphological, characteristic group and thermal stability of nanofiber membranes were studied through scanning electron microscopy (SEM), Fourier transform infrared spectoscopy (FTIR) and differential scanning calorimeter (DSC) respectively. The physical and mechanical properties were also evaluated. Results revealed that the addition of GGM did not significantly affect the rheological properties of electrospinning solution. Increase in the amount of GGM in the nanofiber membrane resulted in gradual smoothening, uniformity and decrease in the number of nodes. KGM interacts with GGM through hydrogen-bond. Addition of GGM markedly enhanced the thermal stability, physical and mechanical properties of the nanofiber membrane. The study showed that the KGM/GGM nanofiber membrane have good potential for use in developing membrane based materials.
Yi, Jeong Wu,Park, Jaesung,Kim, Kwang S.,Kim, Byeang Hyean Royal Society of Chemistry 2011 Organic & biomolecular chemistry Vol.9 No.21
<P>In this paper, we demonstrated a highly discriminated and reliable molecular switch based on the interaction between the self-duplex of <SUP>Py</SUP>A-substituted oligodeoxyadenylate and graphene oxide in aqueous solution. This system showed a clear on/off state through the association and dissociation of <SUP>Py</SUP>A-modified oligodeoxynucleotide with graphene oxide in manipulated pH conditions, high amplitude efficiency for at least 50 cycles, and rapid response within seconds. Our molecular switch system has high reproducibility and simple operation by using pH stimulus.</P> <P>Graphic Abstract</P><P>We demonstrated a highly discriminated and reliable molecular switch based on the interaction between the self-duplex of <SUP>Py</SUP>A-substituted oligodeoxyadenylate and graphene oxide. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c1ob06037f'> </P>
( Yi-xiao Ma ),( Xiao-han Wu ),( Hui-shi Wu ),( Zhan-bo Dong ),( Jian-hui Ye ),( Xin-qiang Zheng ),( Yue-rong Liang ),( Jian-liang Lu ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.7
The degradation efficiency and catabolism pathways of the different methylxanthines (MXs) in isolated caffeine-tolerant strain Pseudomonas putida CT25 were comprehensively studied. The results showed that the degradation efficiency of various MXs varied with the number and position of the methyl groups on the molecule (i.e., xanthine > 7-methylxanthine ≈ theobromine > caffeine > theophylline > 1-methylxanthine). Multiple MX catabolism pathways coexisted in strain CT25, and a different pathway would be triggered by various MXs. Demethylation dominated in the degradation of N-7-methylated MXs (such as 7- methylxanthine, theobromine, and caffeine), where C-8 oxidation was the major pathway in the catabolism of 1-methylxanthine, whereas demethylation and C-8 oxidation are likely both involved in the degradation of theophylline. Enzymes responsible for MX degradation were located inside the cell. Both cell culture and cell-free enzyme assays revealed that N-1 demethylation might be a rate-limiting step for the catabolism of the MXs. Surprisingly, accumulation of uric acid was observed in a cell-free reaction system, which might be attributed to the lack of activity of uricase, a cytochrome c-coupled membrane integral enzyme.