거주자 감성을 위한 주거환경 평가도구 개발 연구

강혜은,이윤희,연태경,장지혜,이연숙 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1

The purpose of this study was to develop the evaluation instrument for sensible aspects of housing environment. In terms of importance of sensibility which people persceive their housing environment, researches on the sensible environment has been done in several academic fields. However, evaluation tool for sensible aspects of housing environment has not existed until now. Therefore, there is a need to develop evaluation tool to measure the fit between dwelling environment and human sensibility. For this purpose, this study reviewed literatures and test results about ergonomics and human factors according to five senses. Based on keywords about five senses and housing environment, a checklist was developed.

복수과 진료 환자의 중복 처방에 대한 분석 및 평가

이지은,장혜경,오지영,유윤경,김현지,임숙인,연숙희,강진숙,최귀령 한국병원약사회 2003 병원약사회지 Vol.20 No.1

In an aging society, there is an increasing possibility of the duplication of the drugs given to patients because they take many kinds of drugs. Among the prescriptions given to the patients who was treated at multiple ambulatory clinics in St. Mary's hospital for one month of September, 2002, in which drugs that has identical or similar effects are prescribed, we analysed and evaluated them by patients' characteristics, kinds of medication, severity of side effects on a case by case basis. More, we assessed the potential additional costs. As a result of this study, the duplication rate turned out to be 6.69% and it was shown that the proportion of the elderly patients over 60 was high. The gastro-intestinal medications took up a large part and most of the cases showed that the danger caused by double taking of medicine was slight, but there were some examples in which the serious side effects were predicted. Expected additional costs were the average 11.349 won and it ranges from the minimum of 56 won to the maximum of 135,720 won. In conclusion, the management of drug histories of the patients who need the plural treatments is very important and the necessity of the individual and professional guidance of taking medicines for the elderly patients is emerged, too.

관해유도 항암요법을 받는 백혈병 환자에서 진균 감염증의 예방 : 무작위 배정법과 이중 눈가림법에 의한 Fluconazole과 Nystain의 효과에 관한 다기관 공동연구 A Randomized, Double-blind, Multicenter Trial to Compare Fluconazole with Nystatin

최강원,오명돈,배현주,백경란,박선양,김병국,신완식,강문원,진종률,박종원,김춘추,김동집,한지숙,민유홍,이선주,고윤웅 대한화학요법학회 1993 대한화학요법학회지 Vol.11 No.2

Fluconazole의 진균 감염증 예방 효과와 안전성에 관하여 3개 대학병원에서 관해유도화학요법을 받는 급성 백혈병환자를 대상으로 무작위 배정법과 너도나도 누가림법에 의하여 연구하였다. 모두 62명의 환자에게 fluconazole(100㎎ bid) 또는 nystatin(1,000,000IU/day)을 무작위로 투여하였다. 투약은 관해유도화학요법과 같은 날짜에 시작하여 호중구수가 1,000㎣이상으로 회복되거나 진균 감염증이 확인되거나 의심되어 Amphotericin-B를 시작하거나, 약과 관련된 부작용이 나타날 때까지 계속하였다. 진균 colonization은 fluconazole군에서 감소하였으나 nystqatin군에서는 증가하였다, 표재성 진균감염증으로 nystatin군에서 C. albicans 진균혈증 1례와 C.parasilosis 진균혈중 1례가 발생하였다. 경험적 항진균요법으로 Amphotericin-B를 투여한 경우는 fluconazolerns 34명중 7례(21%), nystatinrns 28명중 10례(36%)였다(p<0.05). Fluconazole군과 nystatin군 사이에 부작용이나 사망률에 차이는 없었다. 결론적으로, fluconazole은 관해유도화학요법을 받는 급성 백혈병환자에서 진균의 colonization을 줄이는데 효과적이고 안전한 항진균제이다. We made a randomized, double-blind, multicenter trial to compare the efficacy and safety of fluconazole with nystatin for prevention of fungal infections in patients with acute leukemia. Sixty-two adult undergoing remission induction chemotherapy for cute leukemia were enrolled. Patients were randomly assigned to receive either fluconazole (100㎎ bid) or nystatin(1,000,000IU×6/day) with corresponding placebo. The study drug was started in initiation of chemotherapy and continued until recovery of neutrophil counts(>1,000/㎣), development of proven or suspected invasive fungal infection, or the occurrence of drug-related toxicity. Fungal colonization decreased in fluconazole(F) group, however increased in nystain(n) group. Superficial fungal infection occurred in 1 of 34 F group, whereas invasive fungal infection developed in 3 of 28 N group. Empirical amphotericin-B therapy was given in 7 of 34(21%) F group and 10 of 28(36%) N group(p>0.05). The incidence of drug-related side effects and overall moratlity were similar in both study groups.

학문 분야별 감성의 적용사례 분석 연구

장지혜,이윤희,연태경,강혜은,이연숙 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1

The purpose of this study was to analyze application cases of human sensibility in various fields. Nowadays in various fields human sensibility and ergonomic have been studied using variety of methods. Studies on human sensibility have been widely carried in various field such as design and making instruments, selling products and marketing skills even if environmental design fields and human interface by computers. The results have been well applied in of industry. Therefore, understanding rationality. Through this study, ways of creating and measuring environment to improve our living quality, and living places can be developed. From such a viewpoint, this study did the following; First, it reviewed the literatures of studies and results of the tests for application of human sensibility in various field, and organized contents of studies and measurement tools which were used in the studies or tests. Second, it summarized the measurement methods of human sensibility according to three parts; physiological measurement, psychological measurement, and physical measurement. This study was expected to braden the sight on research methodologies and relevant applicable areas.

염색체 위치 특이적 삽입과 안정적인 유전자 발현을 유도하는 플라스미드 백터의 제작

문영준,강윤성,최지혜,손진숙,민나영,이광호 中央大學校 基礎科學硏究所 2002 基礎科學硏究所 論文集 Vol.16 No.-

Insertion of reporter constructs into the mammalian genome leads to variable gene expression due to position effects at the site of integration. This random integration has limited the gene therapy of human genetic disorders by its undesirable effects. We report here the newly constructed plasmid vector(pIRES-neo-YJ) based on the concepts of homologous recombination and position-independent promoter enhancing of beta-globin matrix attachment region(Glb-MAR). chromosome 7 centromere-specific alpha satellite(alphoid) DNA sequence was cloned into pIRES-neo-YJ for homologous recombination of the cloned gene with the centromeric region of chromosome 7, which is genetically silent. Beta Glb-MAR sequence that allows high levels of transcription independent of the chromosomal site of integration was also inserted into pIRES-neo-YJ to ensure the stable and higher expression of the cloned genes. We expect that pIRES-neo-YJ would provide a valuable tool to eliminate random integration of cloned genes into the undesirable chromosomal region and their short-lived expression which often encounters during the construction of transgenic animals and human gene therapy.

제한효소 생성능을 지닌 알칼리성 Bacillus sp. 8-13 균주로부터 알칼리성 제한효소의 정제

배무,이지은,박경숙,이강만 이화여자대학교 생명과학연구소 1992 생명과학연구논문집 Vol.3 No.-

토양에서 분리한 여러 알카리성 세균을 대상으로 제한효소의 유무를 조사하여 Bacillus sp. 8-13으로부터 제한효소의 활성을 발견할 수 있었다. 이 균주의 제한효소는 streptomycin sulfate 침전, ammonium sulfate 침전, DEAD-cellulose와 phosphocellulose ion exchange column chromatography 과정을 통하여 정제하였고 0.1% SDS를 포함하는 7.5% polyacrylamidegel electrophoresis로 subunit의 분자량을 조사한 결과 약 32,000 dalton이었다. 또 특히 이 균주는 형태적 배양학적 특성이 Bacilus alkalophilus subsp. halodurans와 매우 유사하며, 최적 생육 pH는 10.3이었고, 최적 증식온도는 50℃이었다. Twenty-four bacterial strains among alkalophillic bacteria isolated from soil samples were examined for the presence of type Ⅱ restriction endonuclease in aerobic culture. One strain was found to contain specificenzyme to cleave lambda DNA. The characteristics of this microorganism is the ability to grow well in alkalophilic and high temperature condition, that is at pH 10.3 and 50℃. This strain was tentatively identified to Bacilus alkalophilus subsp. halodurans when morphological, physiological and biochemical characteristics were examined. The enzyme was purified from crude extract by streptomycin sulfate, ammonium sulfate precipitation, which was followed by DEAE-cellulose and phosphocellulose ion exchange column chromatography, and the subunit molecular weight was about, 32,000 daltons by polyacrylamide gel electrophoresis containing 0.1% SDS.

A Case of Granuloma Faciale Showing Unusual Histopathological findings

Kang, Sook-Kyoung,Jee, Min-Sun,Lee, Mi-Woo,Choi, Jee-Ho,Sung, Kyung-Jeh,Moon, Kee-Chan,Koh, Jai-Kyoung The Korean Dermatological Association 2002 Annals of Dermatology Vol.14 No.3

Granuloma faciale is an uncommon localized form of small-vessel vasculitis characterized by single or multiple lesions on the face in middle-aged whites, especially males. Histopathological examination demonstrates a dense polymorphous cellular infiltrate consisting of neutrophils, eosinophils, lymphocytes and histiocytes in the upper two thirds of the dermis. A characteristic subepidermal Grenz zone separates the infiltrate from the epidermis. We report a case of granuloma faciale, showing interesting evidence of palisades granuloma-like pattern in the serial biopsy specimen at 3-year interval.

Antioxidant Effect of Salvia miltiorrhiza

Kang, Hye-Sook,Chung, Hae-Young,Jung, Jee-Hyung,Kang, Sam-Sik,Choi, Jae-Sue The Pharmaceutical Society of Korea 1997 Archives of Pharmacal Research Vol.20 No.5

A strong antioxidant activity, which was measured by the radical scavenging effect on 1, 1-diphenyl-2-picrylhydrazyl(DPPH) radical, was detected in the methanol extract of Salvia miltiorrhiza Bunge (Labiatae). By activity-directed fractionation, compounds 1 and 2 were isolated as antioxidant principles of S. miltiorrhiza. Compounds 1 and 2 were identified as dimethyl lithospermate and 3-(3, 4-dihydroxyphenyl)lactamide, respectively, on the basis of spectral data. The radical scavenging effect of compounds 1 and 2 on DPPH radical exceeded that of L-ascorbic acid which is a well known antioxidant. These two compounds also showed prominent inhibitory activity against free radical generation in dichlorofluorescein (DCF) method and cytoprotective effect against t-BHP in cultured liver cell.

Molecular characterization of low-temperature inducible genes from mungbean

Jee-Sook Kang,Eunsook Chung,Chang-Woo Cho,Hyun-A So,Kyoungmi Kim,Jai-Heon Lee 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10

We isolated two low temperature-inducible cDNAs designated as MLT5 and MLT31 from mungbean by subtractive suppression hybridization method. By rapid amplification of cDNA end technique, the full-length cDNAs designated MLT5 and MLT31 were obtained. The full-length cDNA of MLT5 contains an open reading frame of 324 nucleotides coding for 107 amino acids. The full-length cDNA of MLT31 contains an open reading frame of 444 nucleotides in length and capable of specifying a 16.5-kDa protein of 148 amino acids (aa) with an isoelectric point of 7.72. RNA expression of MLT5 was strongly induced by low temperature in the early time and also by wounding, NaCl and ABA. MLT31 mRNA was induced by NaCl and ABA but not by wounding and low temperature stress. MLT5 encodes a protein of unknown function, of which targeting site is predicted to be chloroplast membrane protein. To examine the localization of MLT5, MLT5-GFP was expressed in tobacco cells. It was shown that MLT5-GFP was localized to surface of chloroplast in tobacco cell. To examine the function of MLT31, MLT31 was expressed in Escherichia coli as His-fusion protein. Purified MLT31-His recombinant protein will be tested for ubiquitination activity in vitro. In addition, for the in vivo functional analysis of MLT31, MLT31 will be expressed in yeast ubc9 knock-out mutant. We propose that MLT5 and MLT31 play an important role in signal pathway of abiotic stress in plants.

A Comparative Study between Adolescent Acne and Post - adolescent Acne

(Sook Kyoung Kang),(Min Sun Jee),(Se Jin Ahn),(Jee Ho Choi),(Kyung Jeh Sung),(Kee Chan Moon),(Jai Kyoung Koh) 대한피부과학회 2002 Annals of Dermatology Vol.14 No.3

N/A Background : The prevalence of patients over the age of 25 years with acne has significantly increased over the past 10 years. Several etiologic factors such as genetic factor, hyperandrogenism, stress, and cosmetics have been studied. Objective : The purpose of this study is to evaluate the differences between adolescent acne and post-adolescent acne, based on the clinical features, associated diseases and aggravating factors of 327 acne patients. Methods : 327 consecutive patients, first visited to our hospital with clinical facial acne between March 2000 and February 2001, were enrolled. 220 adolescent patients(AP) were under the age of 25 and post-adolescent patients(PP) were over the age of 25. Results : 1. In the face, cheeks and forehead in adolescents, chin and cheeks in post-adolescents were predilection sites. The predominant type was a comedonal type in adolescents and a papulopustular type in post-adolescents(p<0.05). Severity of inflammatory type was not different between two groups. Familial factors were important in both groups, but more frequently found in post-adolescents with persistent acne(p<0.05). 2. Rosacea, chloasma, and hyperandrogenic features such as hirsutism and androgenetic alopecia were more frequently observed in post-adolescents. Seborrheic and atopic dermatitis were more frequent in adolescents. Four patients had all the features of chloasma, acne, rosacea, seborrheic dermatitis and hirsutism. 3. Aggravation by season especially summer, sweating, stress, and cosmetics was more frequent in adolescents. In PP, acne was more frequently aggravated by sunlight, foods and menstrual period. Conclusion : Post-adolescent acne was mainly a papulopustular type predominantly located on the chin and cheeks with mild to moderate severity, and family history and hyperandrogenism were strong etiologic factors in persistent acne. Aggravating factors were not significantly different between two groups, which suggested cosmetics and stress might not be important in postadolescent acne.