상처치유시 Antisense TGF-β1 Oligodeoxynucleotides가 Inducible Nitric Oxide Synthase발현 조절에 미치는 영향

최병민,곽현정,전창덕,임정식,박석돈,정헌택 大韓免疫學會 1996 大韓免疫學會誌 Vol.18 No.4

Wound healing in the skin may be complicated by both microbial invasion and inflammation. Following injury, platelet degranulation releases a number of chemotactic factors including transforming growth factor (TGF-fl) and platelet derived growth factor (PDGF). TGF-19 is a cytokines that modulates many cellular function and gene expression. However, it is not known whether TGF-P could regulate inducible nitric oxide synthase (iNOS) gene expression during wound healing. Here we report that down regulation of TGF-Q gene expression by antisense oligodeoxynucleotides increase iNOS during wound healing. Our reverse transcription- polymerase chain reaction (RT-PCR) result has shown that antisense TGF-fil oligodeoxynucleotides (ODN) targeting the TGF-J11 translation initiation region markedly reduced TGF-(il mRNA levels in wounded skin. Also, marked reduction in TGF-fl1 mRNA after antisense treatment invited that the increment of iNOS mRNA in wounded skin. Therefore, our irnmunohistochemical studies revealed a "pattern of iNOS product in wounded skin treated antisense TGF-fl1 ODN that was similar to the pattern of RNA synthesis detected by RT-PCR. In conclusion, our results indicated that the regulatory actions of TGF fl1 on iNOS. might be involved in the initiation and faciliates the resolution of inflammation following wounding.

웹 기반 사이버 코스웨어 저작 도구의 설계 및 구현

최길림,김현주,이재동 慶南專門大學 2001 論文集 Vol.29 No.1

소음 작업장에서 근무하는 난청 유소견 근로자들의 소음성난청에 대한 인식 및 태도

최현림,이원철 大韓産業醫學會 1996 대한직업환경의학회지 Vol.8 No.1

This study was designed to survey the knowledge and attitude towards the noise-induced hearing loss (NIHL) of the workers with hearing impairment who are working at the noisy workplaces. The subjects were 423 workers selected from noisy workplaces, where the noise level was 85dB and over, and whose hearing impairment was 30dB and over at 1,000 Hz or 40 dB and over at 4,000 Hz in the primary screening auditory test. For this study, a questionnaire was applied to the study subjects studying their knowledge and attitude towards the noise-induced hearing loss including their personal characteristics. Only 379 workers completed the questionnaires sincerely except 18 workers who did not show hearing impairment, and they were divided into three groups according to their status of hearing impairment: noise-induced hearing loss (D₁), suspected hearing loss (C), hearing loss with medical reasons (D₂), for their comparison of their knowledge and attitude towards the noise-induced hearing loss. The workers who took auditory test at employment were 47.8% and who took auditory test last year after employment were 76.8%. The workers who put on protection device after the test in 77.1%. The workers did not know the fact that they would work at the noisy workplace in 31.9%. The disturbance of daily communication is significantly different symtom among 3 groups (p<0.01). The workers answered that noise did not affect the body adversely in 4.7% and NIHL was not problem if it did not disturb daily life in 31.9%. In case they were diagnosed as NIHL, 68.6% of the subjects answered that they would put on protection devices throughly and 20.8% answered that they would ask for medical care. And 39.3% of them answered that they would want to stay at their present workplaces even though they were ordered to change their workplaces to the another less noisy workplaces. The protection of right answer in the article related NIHL was 61.2% in average. For the protection of NIHL, an effective hearing conservation program should be developed and provided to the labor working in the noisy workplace.

구개결 언어에 대한 Screening test와 설문조사의 비교분석

임재석,권종진,장현석,박영준,최철민,전상호,장수정 大韓顎顔面成形再建外科學會 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.6

Purpose : This study was carried out to evaluate the abnormalites of the speeches of the cleft patients and compare the results of the speech evaluations of a speech pathologist with those of parents. Materials and Methods: Eleven cleft patients' parents were questioned about their children's speech and then the speeches of the cleft patients were evaluated by a speech pathologist and their results were compared with those of parents. Results : Nasal emission was noted in twenty percent of patients, with thirty percent demonstrating hypernasality and all demonstrating no hyponasality and aspirate. And twenty percent of patients show hoarseness. There was no statistically significant difference in the sensitivities to nasal emission between the speech pathologist and parents (p>0.05). However the speech pathologist is more sensitive to hypernasality than parents(p<0.05).

족관절 내과골절에서 피질골 나사못을 이용한 변형된 긴장대 고정술

최호림,도현우,김병흠,김규현,박종석,송준민 대한골절학회 2004 대한골절학회지 Vol.17 No.4

목적 : 전위된 족관절 내과골절에 대해 피질골 나사못을 이용한 변형된 긴장대 고정술을 이용한 치료결과를 분석해 보고자 하였다. 대상 및 방법 : 2001년 1월부터 2003년 1월까지 전위된 내과골절에 대해 변형된 긴장대 고정술을 시행 받고 최소 1년 이상 추시관찰 가능 하였던 24례를 대상으로 하였다. 남자 13례, 여자 11례로 평균 연령은 46세였다. 골절은 Lauge-Hansen 분류법을 사용하였으며, 결과는 Meyer와 Kumler의 평가 방법을 이용하였다. 결과 : 13례 (54%)에서 우수의 결과를 얻었고, 9례 (38%)에서 양호의 결과를 얻었으며, 운동범위의 제한이 발생한 1례에서 보통, 후외상성 관절염이 발생한 1례에서 불량의 결과를 얻었다. 결론 : 전위된 족관절 내과골절에서 피질골 나사못을 이용한 변형된 긴장대 고정술은 견고한 내고정과 조기 관절 운동이 가능한 효과적인 수술 방법이 될 수 있을 것으로 사료된다. Purpose : To evaluate the clinical results of modified tension band wire technique using cortical screw for treatment of displaced medial malleolar fractures of the ankle. Materials and Methods : From January 2001 to January 2003, 24 patients were treated by modified tension band wiring using cortical screw for medial malleolar fracture. The follow-up period was 12~35 months (average 18 months). There were 13 males and 11 females, and the mean age was 46 years. Fractures were classified by Lauge-Hansen's classification. The results were analyzed by Meyer and Kumler's criteria. Results : There were 13 cases (54%) of excellent, 9 cases (38%) of good, and one case of fair because of limitation of motion of the ankle joint and one case of poor which showed post-traumatic arthritis of the ankle. Conclusion : Modified tension band wire technique using cortical screw can be an effective operative method for the treatment of displaced medial malleolar fractures of the ankle.

W^(sh)/W^(sh) 마우스 고환에서 Protein Kinase C(PKC)와 c-kit의 발현 양상

곽현정,최병민,서기석,임정식,정헌택 大韓免疫學會 1996 大韓免疫學會誌 Vol.18 No.3

Protein kinase C (PKC) is a multigene family of at least nine serine/threonine kinases that are central to many signal transduction pathways. And the expression of PKC gene is strictly con-trolled by developmental stage. Mutation of the W (c kit) gene, which encodes a transmembrane tyrosine kinase receptor, affect the development and differentiation stem cells. Most homozygous W mutant mice are sterile, due to a lack of germ cells arising during embryonic development, but one of notable exception is W */Wh mice, which are fully fertile in both sexes. In order to elucidate the biological functions of PKC S and c-kit in the testes, we have examined the expression of PKC b mRNA and c-kit mRNA in While mouse testes by means of Northern blotting. North-em blot results was demonstrated that only PKC 8, 4, 8 mRNA can be detected in normal mouse testes. PKC, 8 mRNA was weakly expressed in 7 and 8-week-old mice and highly ex-pressed by 12 weeks. The PKC 8 mRNA and the c-kit mRNA was detectable in the testes of Whl Wh mouse. These results indicated that the function of the PKC a gene is necessary for the development and the W (c-kit) gene is important for the transmission of signal in testes.

만성 이황화탄소 중독의 판정에 관련된 요인

김병성,최현림,원장원 大韓産業醫學會 1997 대한직업환경의학회지 Vol.9 No.1

The purpose of this study was to compare the characteristics between chronic CS₂poisoning group and non-poisoning group, to find out major factors for decision of chronic CS₂poisoning, to describe the characteristics of cerebral infarctions, and to find out the related factors of cerebral infarctions. The study subjects were 220 ex-employees from W company and the study period was from August, 1993 to November, 1995. The proportion rate of chronic CS₂poisoning was higher in older age groups and longer work duration groups. The rate differed by cerebral infarction, microaneurysm, other retina lesions, polyneuropathy, psychiatric lesions, sensorineural hearing loss. The cerebral infarction was found to be mainly multiple and the frequently occurred locations were periventricular white matter, frontal lobe and basal ganglia. The logistic regression for chronic CS₂poisoning showed that the major factors were microaneurysm, cerebral infarction, polyneuropathy, other retina lesions, diastolic blood pressure, and sensorineural hearing loss. Among the approved group 117 patients were followed up continuously at the outpatient clinic, 62.4% of the patients had hypertension, 61.5% had polyneuropathy, 52.1% had cerebral infarction, 35.0% had hyperlipidemia, 29.9% had liver disease, 15.4% had psychiatric diseases, 6.0% had angina, and 4.3% had anemia. For the hypertensive patients, 1.9 kinds of antihypertensive drugs were needed to control the blood pressure, and the BP decreased from 164/101 mmHg to 131/81 mmHg.

컨테이너 터미널 ERP 시스템 구축 방안

이상완,최형림,박남규,김현수,박병주,유동호 한국로지스틱스학회 2002 로지스틱스연구 Vol.10 No.1

급속한 세계화로 터미널간의 경쟁이 치열해지면서 기존 시설의 효율적인 운영 및 관리가 터미널의 경쟁력과 생산성을 향상시키는 중요한 요소로 부각되고 있다. 하지만 우리나라 컨테이너 터미널은 양적 확장에 치중한 반면 관리와 운영의 효율성에 대해서는 다소 소홀히 해 왔다. 그리고 대부분의 컨테이너 터미널은 자사 운영시스템을 저문 업체에 의뢰하여 개발함으로써 컨테이너 터미널마다 상이한 시스템이 구축되어 시스템의 유지 보수비용이 많이 발생하고 있다. 최근 터미널 개발과 운영 자체가 사업화 되고 있기에 향후 터미널 운영시스템에 대한 수요는 증가할 것이다. 그래서 터미널마다 다른 운영시스템을 표준화하고 국제화한 ERP 형태의 통합 운영 시스템의 개발이 요구된다 하겠다. 따라서 본 연구에서는 주문형 방식에 의존하던 컨테이너 터미널 운영시스템의 고비용, 비 통합화, 개발기간의 장기화 등의 단점을 극복하기 위해 컨테이너 터미널 ERP 시스템이란 새로운 개념을 제안한다. 이 컨테이너 터미널 ERP 시스템은 통합된 운영시스템 구축, 개발기간의 단출, 개발비용의 절감, 쉬운 업그레이드를 가능하게 해 컨테이너 터미널의 글로벌 사업에 전략적 도구로써 역할을 하게 될 것으로 기대된다. The major characteristics of ERP(Enterprise Resource Planning)are enterprise-wide system that covers all the business functions and information resources, integrated database, built-in best industry practice, packaged software and open architecture. ERP enables reduction of system development time, flexibility, standardization of workflow and effective business planning capability. ERP is mainly for manufacturing industry. However, the principles of ERP can also be applied to container terminal. We have clustered the workflow of container terminal, and analyzed the business process to generate the best workflow. The integrated database is designed to eliminate redundancy and keep integration. The core of ERP for container terminal is the planning facility such as berth planning and yard planning. The planning capability is very tightly coupled with data flow from client entities such as shipping companies. The ERP can handles the existing problems of container terminal operation that are mainly caused by lack of integration of whole of information resource in container terminal, ad-hoc and poor planning capability, disconnected and incorrect data from client companies. ERP approach not only van resolve the problems of container terminals but also promote adoption of information systems for container terminals in the world that have not yet implemented terminal operation systems.

일개 대학병원에 내원한 장애자의 실태

김병성,장인서,최현림,원장원 경희대학교 2001 慶熙醫學 Vol.17 No.1

레이온회사 퇴직근로자에서 이황화탄소에 의한 건강영향평가 : 직업병 인정여부에 따른 자각증상과 관련 요인 Subjective Symptoms and Related Factors According to Compensation for CS_2 Poisoning

이경재,김주자,최현림,양길승,김호,임상혁,이윤근 大韓産業醫學會 2003 대한직업환경의학회지 Vol.15 No.2

목적: 이 연구는 이황화탄소 취급 사업장에서 근무한 경력이 있는 퇴직 근로자들을 대상으로 이황화탄소에 의한 건강영향을 조사함으로써, 이황화탄소 노출 후의 건강영향 파악 및 이황화탄소 노출 근로자에 대한 건강관리 기준 마련 등에 중요한 기초자료를 제공하고자 하였다. 방법: 이 연구는 우리나라 모 레이온회사에 근무한 경력이 있는 퇴직 근로자 중에서 1998년 12월까지 직업병 여부를 확인하기 위해 건강진단을 신청한 1,702명 중에서 이미 사망한 40명을 제외한 1,662명을 대상으로 하여 이 연구 목적에 대한 설명문을 우편으로 발송하여 참여 의사를 밝힌 258명을 면접대상으로 하여 최종적으로 177명을 분석 대상으로 하였다. 결과: 연구대상자는 남자가 147명, 여자가 30명이었고, 평균 연령은 55.4세이고 입사시 연령은 29.1세였다. 근무부서별로는 고폭로부서에 근무한 군이 156명으로 88%를 차지하였으며, 인정받은 군이 103명(58.2%), 받지 못한 군이 74명(41.2%)이었다. 평균 총폭로지수는 38.96(±31.18)이었고, 인정받은 군에서 받지 못한 군 보다 유의하게 높았고 (p=0.000). 고폭로부서군에서 저폭로부서군보다 높게 나타났다(p=0.000). 퇴사전후 시간에 따른 호소 증상들을 분석한 결과, 퇴사전후 시간 흐름에 따라 호소하는 증상들이 차이는 있지만 호소하는 증상들이 퇴사전과 퇴사후 5년 이내에 비해 퇴사후 5~10년인 경우에는 인정받은 군과 받지 못한 군 간에 유의한 차이를 보이는 호소 증상들이 적어지는 경향을 보였다. 그러나 퇴사 10년 이상인 경우에는 퇴사후 5~10년 이내에 비해 오히려 호소하는 증상들은 많아진 반면, 인정받은 군보다 받지 못한 군에서 유의하게 높았다. 따라서 이는 퇴사후 10년 이상이 되면 그 이전 기간에 비해서 호소하는 증상들은 직업병 인정에 올바른 영향을 미치지 않을 수도 있다고 생각되고 이러한 결과에 영향을 미칠 수 있는 여러 가지 요인들을 생각해 볼 필요가 있다고 생각된다. 인정받은 군에 대해서 직업병 인정시점을 기준으로 인정전후에 호소 증상의 변화를 비교한 결과, 대부분 직업병 인정전에 비해 인정후에 호소하는 증상이 감소하는 경향을 보이고 있어 직업병 인정후에 의료기관으로부터 증상관리가 이루어지는 것이 증상호소를 줄이는 데 기여한 것으로 생각되며 이밖에도 직업병 인정으로 인한 심리적인 보상도 영향을 미칠 수 있을 것으로 생각된다. 직업병 인정여부에 영향을 주는 요인에 관하여 분석한 결과, 총폭로지수와 퇴사후 최종 건강진단을 받을 때까지의 시기가 유의한 요인으로 나타났으며, 퇴사후 직업병 인정을 받을 때까지의 시간에 대한 Cox의 비례위험 회귀분석을 한 결과, 총폭로지수와 입사연령, 고폭로부서의 근무여부가 의미있는 요인으로 나타났다. 결론: 이 연구를 통하여 이황화탄소 취급 사업장에서 근무한 퇴직 근로자들을 대상으로 직업병 인정에 따른 호소 증상을 분석하고 직업병 인정에 영향을 주는 요인을 분석해 봄으로써 이황화탄소 노출에 의한 건강영향을 파악하고 폭로 이후의 증상변화를 예측 관리할 수 있는 자료를 마련하고자 하였으며 이는 이황화탄소 노출 근로자들에 대한 건강관리에 기초자료로 의미를 가질 것으로 생각된다. Objectives: To investigate the chronological subjective symptoms and related factors in exworkers who were occupationally exposed to Carbon disulfide. Methods: One hundred and seventy-seven subjects(147 males and 30 females) were randomly selected among ex-workers and interviewed by well trained doctors, who filled out a structured questionnaire developed by authors on the subjective symptoms. The questionnaire was composed of symptoms in the head and neck, and those of the neuro-psychological, respiratory, endocrine, musculoskeletal, and reproductive systems. Other factors such as general and occupational characteristics including work department, work duration, duration since cessation of exposure, and compensation for occupational disease were also investigated. Results: The mean subject age was 55.4 years. The mean cumulative exposure index (CEI) was 38.96±31.18. CEI was significantly higher in the compensated group(47.61±32.51) than in the non-compensated group(26.81±24.75)(p=0.000). Symptom complaints were significantly higher in the compensated group in all organ systems, and their incidence increased gradually until 10 years after cessation of work, and then decreased. The mean duration for cessation of exposure at the time of compensation was 10.97 years. The significantly different factors between the compensated group and the non-compensated group were total cumulative exposure index and the time interval between the cessation of work and the time of compensation. Conclusion: Our findings suggest that total cumulative exposure index and the time interval between the cessation of work and the time of compensation may be important factors affecting the compensation for occupational disease. Therefore, further studies will be necessary.