Bacillus sp. FF-7에 의한 항산화물질 생산조건과 항산화 활성

차재영,김효정,전방실,박진철,옥민,조영수 한국응용생명화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.3

발효식품 시료로부터 분리된 세균을 DPPH(α,α¹-diphenyl-β-picrylhydrazyl) 전자공여능으로 항산화 활성을 측정하여 가장 활성이 강한 균주를 선별하여 형태학적, 생화학적, 생리학적 특성 및 16S rRNA 염기서열을 조사한 결과 Bacillus sp.으로 판명되어 FF-7로 명명하였다. DPPH 전자공여능법에 의한 Bacillus sp. FF-7이 생산하는 항산화물질의 최적 생산 배지조건은 탄소원 2% galactose와 질소원 1% tryptone 첨가였다. Bacillus sp. FF-7에 의해 생성된 항산화 물질의 활성을 DPPH 전자공여능, 흰쥐 각 조직 microsomal 실험계 및 linoleic acid 과 산화지질 실험계에서 malondialdehyde를 thiobarbituric acid(TBA)방법으로 측정하였다. 흰쥐 각 조직 microsomal 실험계에서 지질과산화에 대한 항산화 효과는 뇌(97.50%)>심장(79.95%)>신장(77.84%)>비장(77.47%)>고환(69.96%)>간장(62.45%) 순이였다. Linoleic acid의 과산화지질률 TBA법으로 측정한 결과 반응 4일째까지 억제 효과가 강하게 나타났으며, 동시에 대조구로 사용한 0.05% BHT 첨가구에서도 실험종료시까지 항산화 활성이 강하게 나타났다. The antioxidative activity of antioxidative substances produced from several bacterial strains isolated from fermented foods were tested by DPPH (α,α'-diphenyl-β-picrylhydrazyl) free radical scavenging activity. One of the strains showing the highest antioxidative activity was identified as Bacillus sp. based on the morphological, biochemical, physiological characteristics, and 165 rRNA sequence, and named FF-7. The molt optimal medium condition for the production of antioxidative substance from Bacillus sp. FF-7 was 2% galactose as carbon source and 1% tryptone as nitrogen source. The antioxidative substance produced from FF-7 in these cultural medium was also tested by in vitro experimental models, the peroxidation of linoleic acid and the peroxidation of rat tissues microsomes by using thiobarbituric acid (TBA) for assay of free rnalondialdehyde production. The antioxidative avtivity against lipid peroxidation of rat tissues microsomes was shown in the following order; brain 97.50% > heart 79.95% > kidney 77.84% > spleen 77.47% > testis 69.96% > liver 62.45%. The antioxidative substance produced from FF-7 on linoleic acid peroxidation by TBA method was effectively inhibited during four days, and 0.05% BHT(butylated hydroxytoluene) used comparative control was also effectively inhibited. Results showed that the highest antioxidative activity by DPPH method of antioxidative substance produced from Bacillus sp. FF-7 was obtained by supplementing 2% galactose as carbon source and 1% tryptone as nitrogen source in cultured medium, this substance effectively inhibited the formation of TBARS in brain microsome in vitro system and in linoleic acid peroxidation.

CXTFIT기법을 이용한 오염토양의 중금속 이동특성에 관한 연구

오광중,김효진,박창웅,오방일 부산대학교 환경문제연구소 2002 環境硏究報 Vol.20 No.-

The sorption and transport characteristics of Cd and Pb is founded by changing of soil concentration and velocity of injected Cd and Pb solution. The results of batch-type experiment indicated that sorption capacity of Pb was stronger than sorption capacity of Cd. Batch-type experiment indicated that the results of Cd and Pb adsorption experiment fitted in the Freundlich isotherm equation well and were highly nonlinear. Results from column experiment showed the retention of the transport of heavy metals at low concentration, low velocity and high pH in soil of different characteristics. The BTCs of the heavy metals were analysed by using a two-site model, and transport parameters were derived by using the CXTFIT curve-fitting program. The model results indicated that the partitoning coefficient(β), forward rate coefficient(Ks) and backward rate coefficient(kd) were in proportion to the concentration, velocity and pH.

미세혈관 협심증 환자의 임상상 및 지표에 관한 연구

박상호,온영근,김현정,이태훈,길효욱,김현건,박수진,송해정,한대희,방덕원,신원용,이내희,김성구,권영주 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.1

Background : Microvascular angina or syndrom X is heterogenous and encompasses different pathogenetic entities. Several studies suggest that microvascular angina have good prognosis and occurs predominantly in postmenopausal women. Lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, homocystein, CRP and fibrinogen are the independent risk factor of acute coronary syndrome and are the chief underlying cause of atherosclerosis. We investigated clinical characteristics and markers in patients with microvascular angina. Methods : We studies 23 patients diagnosed with microvascular angina and 16 control group with negative treadmill exercise test and no significant coronary angiogram. We investigated clinical characteristics in patients group and evaluated symptom during a follow-up period of 4.2± 2.5 month. Exercise time in treadmill exercise test was compared between patients and control group. We assessed the level of markers, such as lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, homocystein, CRP and ESR in two groups. Results : There is no difference in age between two groups. Patients group had a tendency to increase in female, hypertension, DM, and smoking history, but there was no statistical significance difference. Exercise duration time was significantly decreased in patients with microvascular angina (p<0,05). Serum lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholestrol, Triglyceride, ESR, Fibrinogen and Homocystein value were no significant difference between microvascular angina and control group. Only CRP was significantly high in patients with microvascular angina (p<0.05). Conclusion : There was no statistical significance difference in age, sex, hypertension, DM and smoking history between two groups but patients group had a tendency to increase the prevalence in hypertension, DM and smoking history, female. Also, Symptom improvement and disappearance in follow-up was presented in 11 patients of 19(57.9%). Exercise tolerance was worse in patients group than control group. CRP value was significantly higher in patients group than control group. There was no statistical significance difference in lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, homocystein, ESR and Fibrinogen between two groups.

Hepatoprotective effects of Allyl isothiocyanate in CCl4-induced hepatic damage in rats

Hyo Jin Bang,Mee Jung Ahn,Ji Hwan Moon,Giok KIim,Tae Kyun Shin 한국실험동물학회 2015 한국실험동물학회 학술발표대회 논문집 Vol.2015 No.8

Regeneration inverter for DC electric traction system with the capability of reduction of harmonic

Hyo-Jin Bang,Chang-Youl Choi,Su-Jin Jang,Chang-Han Bae,Kyu-Bok Ahn,Chung-Yuen Won 전력전자학회 2004 ICPE(ISPE)논문집 Vol.- No.-

This paper proposes a dc power system, which can generate the excessive dc power from dc bus line to ac source in substations for traction system. The proposed regeneration inverter system for dc traction can be used as both an inverter and an active power filter (APF). As a regeneration inverter mode, it can recycle regenerative energy caused by decelerating tractions and as an active power filter mode, it can compensate for harmonic distortion produced by the rectifier substation. From the viewpoint of both power capacity and switching losses, the system is designed on the basis of three phase PWM inverters output transformers and an LCL filter.

Hepatoprotective effects of allyl isothiocyanate in CCl4-induced chronic hepatic damage in rats

Hyo-jin Bang,Yun-a Choi,Mee-jung Ahn,Jeong-tae Kim,Gi Ok Kim,Tae-kyun Shin 대한수의학회 2016 대한수의학회 학술대회발표집 Vol.2016 No.-

Group

Bang, Hyo Jin 한국콘텐츠학회 2019 ICCC International Digital Design Invitation Exhib Vol.2019 No.8

뷰티메이크업과 아트메이크업 이미지에 따른 인지·감성 평가 비교

방효진 ( Hyo Jin Bang ),김정희 ( Jeong Hee Kim ) 한국미용학회 2013 한국미용학회지 Vol.19 No.2

This study aimed to identify the influence of recognition and emotion response to the images of beauty makeup and art makeup in a variety of advertisements. This study has investigated how cognitive and emotional responses are expressed depending on beauty makeup as well as art makeup images. This study also compared the differences between the two responses by type of makeup against a group of college students. The following results were obtained: In cognitive responses, ``Practicality`` are higher evaluation factor in beauty makeup and ``Artistry`` are higher evaluation factor in art makeup. In emotional response, ``Familiarity`` are higher evaluation factor in beauty makeup and ``Ornamentation`` are higher evaluation factor in art makeup. Therefore, there are contrary to each makeup subdivision in the sensitivity. In accordance with the analysis on the difference of recognition and emotion response depending on makeup styles, the important elements in the art makeup were artistic value in recognition response, decorative value in emotion response. For the beauty makeup, the important elements were practicality in recognition response, familiarity in emotion response, dignity. It indicates the difference in evaluation on those two kinds of makeup styles.

직류전철용 전력공급 장치의 에너지회생에 관한연구

방효진(Hyo-Jin Bang),장수진(Su-jin Jang),송상훈(Sang-Hun Song),원충연(Chung-Yuen Won),김용기(Yong-Ki Kim),안규복(yu-Bok Ahn) 전력전자학회 2004 전력전자학술대회 논문집 Vol.- No.-

This paper described a dc power system, which can generate the excessive dc power form dc bus line to ac source in substation for traction system. The proposed regeneration inverter system for dc traction can be used as both an inverter and an active power filter(APF). As a regeneration inverter mode, it can recycle regenerative energy caused by decelerating tractions and as an active power filter mode, it can compensate for harmonic distortion produced by the rectifier substation.

연구논문 : 20-30대 미혼남성에 대한 여성의 메이크업 이미지 선호도 -결혼 및 연애 상대 선택을 중심으로-

방효진 ( Hyo Jin Bang ) 한국미용학회 2013 한국미용학회지 Vol.19 No.5

This study tried to survey which makeup image of females single males in their 20s and 30s living in Seoul and Daejon prefer in thedecision making to choose a female for love and marriage when they see the four different makeup images of females. Single malespreferred ‘Smoky makeup’ in the choice of a date of love and ‘Natural makeup’ in the choice of a mate in marriage most. Again malesin their 20s preferred ‘Smoky makeup’ most for their date of love, but those in their 30s preferred ‘Sexy makeup,’ whereas for a mate inmarriage males in their 20s preferred ‘Natural makeup’ and ‘Cutie makeup’ most while those in their 30s preferred ‘Natural makeup’ among the rest. Furthermore extroverts preferred ‘Sexy makeup’ wearers as a date of love and ‘Natural makeup’ wearers as a mate inmarriage. But introverts preferred ‘Natural makeup’ most in their selection of a date of love and a mate in marriage identically. There forethe analysis of the results from all the above experiments shows that in general when a single male picks a female date for love, heprefers a luxurious and urban image of ‘Smoky makeup’ and ‘Sexy makeup’ most whereas he prefers a pure and natural image of ‘Natural makeup’ among the rest in time of considering a mate in marriage.