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Reduced Mitochondrial Properties in Putative Progenitor/Stem Cells of Human Keratinocytes
( Sung Eun Chang ),( Young Mi Kim Pak ),( Hae Woong Lee ),( Jee Ho Choi ),( Eun Jeong Jeong ),( Seung Ho Choi ),( Hyo Won Chang ),( Yoo Sam Chung ),( Sang Yoon Kim ) 대한피부과학회 2009 Annals of Dermatology Vol.21 No.4
Background: The characterization of progenitor/keratinocyte stem cells (KSC) remains an unachieved goal. A previous study showed that rapid adhering cells to collagen IV had the characteristics of putative progenitor/KSCs. Objective: The purpose of this study was to investigate the genetic expression of rapid adhering cells compared to non adhering cells to determine the characteristic of KSCs. Methods: We isolated rapid adhering cells representative of KSCs from non adhering cells representative of transient amplifying cells. In addition, we differentiated cells from human tonsilar keratinocytes utilizing the adhering capability of the KSCs to collagen IV. Annealing control primer based differentially displayed polymerase chain reaction (PCR) was performed as well as Western blot analysis. Results: The levels of mitochondria- related gene expression were low in the rapid adhering cells compared to the non adhering cells. Mitochondrial complex I, COX IV, peroxiredoxins (I, II and IV) and mitochondrial membrane potential were all low in the rapid adhering cells compared to the non adhering cells. Conclusion: Using an adhesion method on human collagen IV-coated plates, our results suggest that reduced mitochondrial function may be an important characteristic of KSCs. (Ann Dermatol 21(4) 364~368, 2009)
Hyo Young Jung,Woosuk Kim,Kyu Ri Hahn,Min Soo Kang,Hyun Jung Kwon,Jung Hoon Choi,Yeo Sung Yoon,Dae Won Kim,Dae Young Yoo,In Koo Hwang 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7
We investigated the effects of pyridoxine deficiency on ischemic neuronal death in the hippocampus of gerbil (n = 5 per group). Serum pyridoxal 5’-phosphate levels were significantly decreased in Pyridoxine deficient diet (PDD)-fed gerbils, while homocysteine levels were significantly increased in sham- and ischemia-operated gerbils. PDD-fed gerbil showed reduction in NeuN-immunoreactive neurons in the medial part of the CA1 region three days after. Reactive astrocytosis and microgliosis were found in PDD-fed gerbils, and transient ischemia caused the aggregation of activated microglia in the stratum pyramidale three days after ischemia. Lipid peroxidation was prominently increased in the hippocampus and was significantly higher in PDD-fed gerbils than in CD-fed gerbils after ischemia. In contrast, pyridoxine deficiency decreased the proliferating cells and neuroblasts in the dentate gyrus in sham- and ischemia-operated gerbils. Nuclear factor erythroid 2-related factor 2 (Nrf2) and brain-derived neurotrophic factor (BDNF) levels also significantly decreased in PDD-fed gerbils sham 24 h after ischemia. These results suggest that pyridoxine deficiency accelerates the neuronal death by increasing serum homocysteine levels and lipid peroxidation, and by decreasing Nrf2 levels in the hippocampus. Additionally, it reduces the regenerated potentials in hippocampus by decreasing BDNF levels. Collectively, pyridoxine is an essential element in modulating cell death and hippocampal neurogenesis after ischemia.
( Hyo Jung Cho ),( Jung-dong Lee ),( Dae Yong Kang ),( Bohyun Kim ),( Jei Hee Lee ),( Jai Keun Kim ),( Sung Jae Shin ),( Kee Myung Lee ),( Byung Moo Yoo ),( Kwang Jae Lee ),( Soon Sun Kim ),( Jae Youn 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1
Aims: This study was performed to evaluate long-term outcome of indeterminate nodules detected on cirrhotic liver and to develop risk prediction model for hepatocellular carcinoma (HCC) progression of indeterminate nodules on hepatitis B virus (HBV)-related cirrhotic liver. Methods: Indeterminate nodules up to 2 cm with uncertain malignant potential detected on cirrhotic liver during HCC surveillance were analyzed retrospectively. HCC risk prediction model of indeterminate nodules in HBV-related cirrhotic liver was deduced based on result of Cox regression analysis. Results: A total of 494 indeterminate nodules were included. Independent risk factors of HCC progression were old age, arterial enhancement, large nodule size, low serum albumin level, high serum alpha-fetoprotein (AFP) level, and prior HCC history in all included subjects. In subjects with chronic hepatitis B, old age (year; HR=1.06; P<0.001), arterial enhancement (HR=2.62; P=0.005), large nodule size (>1cm; HR=7.34; P<0.001), low serum albumin level (≤3.5g/dL; HR=3.57; P=0.001), high serum AFP level (≥100ng/mL; HR=6.04; P=0.006), prior HCC history (HR=4.24; P=0.001), and baseline HBeAg positivity (HR=2.31; P=0.007) were associated with HCC progression. We developed a simple risk prediction model using these risk factors and identified patients at low, intermediate, and high risk for HCC; 5-year cumulative incidences were 1%, 14.5%, and 63.1%, respectively. The developed risk score model showed good performance with area under the curve at 0.886 at 3 years, and 0.920 at 5 years in leave-one-out cross-validation. Conclusions: We developed useful and accurate risk score model for predicting HCC progression of indeterminate nodules detected on HBV-related cirrhotic liver.