Recombinant canstatin inhibits angiopoietin‐1‐induced angiogenesis and lymphangiogenesis

Hwang‐,Bo, Jeon,Yoo, Ki Hyun,Park, Jong‐,Hwa,Jeong, Han‐,Sin,Chung, In Sik Wiley Subscription Services, Inc., A Wiley Company 2012 International journal of cancer: Journal internati Vol.131 No.2

<P><B>Abstract</B></P><P>We describe the effect of recombinant canstatin, the NC1 domain of the α2 chain of Type IV collagen, on suppression of angiogenesis and lymphangiogenesis both <I>in vitro</I> and <I>in vivo</I>. Recombinant canstatin produced from stably transformed <I>Drosophila</I> S2 cells reduced the expression of angiopoietin‐1 in hypoxia mimetic agent, CoCl<SUB>2</SUB>‐treated CT‐26 cells. Recombinant canstatin inhibited proliferation, tube formation and migration of human angiopoietin‐1 (rhAngpt‐1)‐treated human umbilical vein endothelial cells (HUVEC) and lymphatic endothelial cells (LEC). Recombinant canstatin suppressed the expression of Tie‐2 and vascular endothelial growth factor‐3 (VEGFR‐3) transcripts in rhAngpt‐1‐treated HUVEC and LEC, respectively. The inhibitory effect of recombinant canstatin on tumor growth was also investigated using a heterotopic CT‐26 colon carcinoma animal (BALB/c mice) model. Recombinant canstatin reduced the final volume and weight of tumors, and blood and lymphatic vessel densities of tumors, which were evaluated by CD‐31 and LYVE‐1 immunostaining. Immunohistochemical analysis showed that recombinant canstatin dramatically reduced the expression of angiopoietin‐1 in CT‐26 colon carcinoma‐induced tumor, but not the expression of VEGF‐C. Tie‐2 and VEGFR‐3 expressions were also reduced in recombinant canstatin‐treated tumors. These results indicate that recombinant canstatin has anti‐tumoral activities against CT‐26 colon carcinoma cells. Recombinant canstatin reduces the expression of angiopoietin‐1 in hypoxia‐induced CT‐26 cells and inhibits the angiogenic and lymphangiogenic signaling induced by angiopoietin‐1. Recombinant canstatin probably inhibits angiogenesis and lymphangiogenesis <I>via</I> suppression of the integrin‐dependent FAK signaling induced by angiopoietin‐1/Tie‐2 and/or VEGFR‐3.</P>

형제 참여 언어중재가 언어장애 아동의 언어능력에 미치는 효과

황보명 한국음성과학회 2003 음성과학 Vol.10 No.3

This study was aimed at clarifying the effects of sibling-involved language intervention in children with language disorders. Ten subjects were selected an assigned into two groups (language intervention group with sibling-involvement, a language intervention without sibling-involvement). Group 1 consisted of 5 children with language disorders. Group 2 consisted of 5 children with language disorders without sibling-involvement. To investigate the language and communication ability, Picture Vocabulary est. and the subjects's spontaneous speech sample were analyzed. To compare pre-treatment with post-treatment between two the groups, repeated-measures ANOVA and t-test were used. The results were as follows; First, the language intervention with sibling-involvement was not effective compared to without sibling-involvement in improving the picture vocabulary ability of children with language disorders. There was a significant difference between pre-treatment and post-treatment in the picture vocabulary ability of the two groups. However, there was no significant difference in the post-trearment between the two groups. Second, the language intervention with sibling involvement was more effective compared to without sibling-involvement in improving the semantic aspects of language of children with language disorders. There was a significant difference between per-treatment and post-treatment in the number of semantic elations between the two groups in that group 1 showed a higher improvement than group 2. Third, the language intervention with sibling involvement was more effective in improving pragmatic aspects of language of children with language disorders. There was a significant difference between pre-treatment and post-treatment in the number of communicative intentions of the two groups. And there was a significant difference in the post-treatment between the groups in that group 1 showed a higher improvement than group 2.

연폭로 근로자들에서 빈혈증 관련 인자에 관한 연구

황보영,김용배,김화성,이성수,안규동,이병국 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

Objectives : This study intended to obtain the interrelationship of lead exposure indices and anemia related factors for the better health management of lead exposed workers. Methods : 502 male workers in storage battery industries as lead exposed group and 106 male office workers as contorl group were selected for this study. The examined variables wer age, work duration, smoking and alcohol crinking habit, blood zinc protoporphyin concentraion (ZPP), blood lead concentration (PbB), urinary δ-aminoevulinic acid (ALAU), hemoglobin (Hb), hematocrit (Hct), serum calcium (S-Ca), serum iron (S-Fe), total iron binding capacity (TIBC), and percentage trasferrin saturation (PTS). Results : The means of ZPP, PbB ALAU in lead exposed proup were 58.4±35.7 ㎍/㎗, 27.6±10.9 ㎍/㎗, and 1.61±2.00 mg/ℓ, respectively. These three values in lead exposed group were significantly higher than control group (P<.05). The means of Hb, Hct, S-Ca, S-Fe, TIBC, PTS in lead exposed group were 15.3±1.1 g/㎗, 47.2±4.5 %, 9.4±0.4 ㎍/㎗, 124.0±67.5 ㎍/㎗, 317.1±68.5 ㎍/㎗, and 38.7±15.2 %, respectively. Hb, Hct, S-Ca, S-Fe, and TIBC in lead exposed group were significantly lower than contrl group (P<.05). PTS was no significant difference in lead exposed group and control group (P> .05). Hct, S-Ca, S-Fe, PTS were significant positive correlation with Hb and ZPP, corrected PbB, AlAU were significant negaitive correlation with Hb in lead exposed group (P< .05). On multiple regression using ZPP as a dependent variable and PbB, S-Ca and S-Fe as independent variables after adjustment for possible confounder such as age, smoking and drinking habit, Pbb, S-Ca, S-Fe and age contributed to ZPP. Conclusions : As the result of this study, it was suggested that inclusion of S-Ca, S-Fe, PTS measurement as a anemia screening test for lead workers was highly recommended for the effective prevention and early diagnosis of anemia.

펄스정전응력법을 이용한 고분자 재료에서 공간전하분표 측정에 대한 이론적 고찰

황보승 호남대학교 산업기술연구소 1998 산업기술연구논문집 Vol.6 No.-

본 논문에서는 최근 국 ·내외적으로 활발하게 연구되고 있는 고체 고분자 재료 내의 공간전하 측정법과 관련하여, 공간전하의 비파괴 측정법 중의 하나인 펄스정전응력법(PEA 법)에 대한 이론적 고찰을 수행하였다. 또한, 두꺼운 압전소자를 이용해서 PEA 법의 공간분해능을 향상시킬 수 있는 새로운 방법을 제안하였다. In this paper, we have evaluated theoretically the PEA method which is one of the most popular methods for the nondestructive measurement of space charge distribution in polymers. On the basis of this theoretical evaluation, we also suggest a new measuring method that uses a thick piexo-material for improving the space resolution of PEA method.

놀이중심의 집단 언어중재 프로그램이 언어장애 아동의 언어능력에 미치는 영향

황보명 한국동서정신과학회 2003 동서정신과학 Vol.6 No.2

본 연구는 놀이중심의 집단 언어중재 프로그램이 언어장애 아동의 언어능력에 어떤 영향을 미치는지에 대해서 알아보고자 하는 것이다. 본 연구자가 정한 기준에 따라 5명의 언어장애 아동을 연구대상으로 선정하였다. 연구는 사전 단계, 중재 단계, 사후 단계의 세 단계로 나누어 실시하였다. 사전 단계에서는 각 연구대상 아동들의 언어능력 평가를 실시하였는데, 부모와의 인터뷰 실시, 관찰 실시, 언어샘플 수집 등의 하위 단계로 나누어 실시하였다. 중재 단계에서는 놀이중심의 집단 언어중재 프로그램을 연구 대상 아동들에게 주 2회기씩, 총 30회기 동안 제공하였다. 사후 단계에서는 사전 단계와 동일한 언어검사 실시 및 언어샘플을 수집하였다. 본 연구에서 사용한 언어장애 아동의 물리적 환경 및 교육적 환경을 고려하여 본 연구자가 고안한 언어중재 프로그램이다. 이 프로그램에서는 총 10가지의 놀이 활동을 제시하였는데, 이것을 다시 회기마다 포함되는 공통 활동 2가지와 회기마다 변화되는 활동 8가지로 구분하였다. 회기마다 포함되는 공통 활동으로는 자유놀이와 집단 시간이 있고, 회기마다 변화되는 활동들로는 수수께끼, 움직이기, 만들기/그리고, 운동게임, 역할놀이, 끝말잇기, 요리하기, 보물찾기가 있다. 연구 대상에 대한 사전-사후 검사 결과 의사소통 의도 다양성, 의미관계 다양성, 수용언어능력, 표현언어능력, 문장이해능력, 언어이해·인지능력, 그럼어휘능력은 유의미한 차이가 있는 것으로 나타났다. 그러나, 어휘다양도에서는 유의미한 차이가 발견되지 않았다. 따라서 본 연구 결과, 놀이중심의 집단 언어중재 프로그램이 언어장애 아동에게 언어능력 중 화용론 측면과 의미론적 측면뿐만 아니라 전반적인 언어능력을 높이는 데 효과가 있음이 증명되었다. The study was aimed at clarifying the effects of a Group Language Intervention in children with language disorders. Five subjects were selected and two speech-language pathologists engaged in the group language intervention. In the groupnal language intervention, activities consisted of group time, playing riddles time, following directions time, drawing time, cooking time, word-chain game time, role-playing time, searching for objects based on directions, and recreation game time. To compare pre-treatment with post-treatment in the children with language disorders, repeated-measures ANOVA was used. The results were as follows; First, the Functional Language Intervention was effective in improving the number of communicative intentions of children with language disorders. There was a significant difference between pre-treatment and post-treatment in the number of communicative intentions. Second, the Functional Language Intervention was effective in improving the number of semantic relations of children with language disorders. There was a significant difference between pre-treatment and post-treatment in the number of semantic relations. Third, the Functional Language Intervention was effective in improving the overall language abilities of children with language disorders. There was a significant difference between pre-treatment and post-treatment in the overall language abilities.

경제적 형광등 교체를 위한 표시장치 개발에 대한 연구

황보승 호남대학교 산업기술연구소 1999 산업기술연구논문집 Vol.7 No.-

본 논문에서는 현재까지 광원의 주류를 이루고 있는 형광등에 의한 불필요한 전력소모를 억제하고 전기요금을 절약하기 위한 최적의 형광등 교체주기 표시에 대한 연구를 수행하였다. 이를 위하여 본 연구에서는 시간경과에 따른 형광등 노화에 의한 소모전력의 증가와 그에 따른 경제성을 분석하였으며, 이를 바탕으로 최적의 교환시기가 되면 자동으로 적색 LED가 소등(또는 점등)되는 장치를 설계 및 개발하였다. 또한 P-spice를 통한 회로 해석과 실험을 통하여 개발된 장치의 정상적인 동작을 확인하였다. In this paper, in order to restrict the power consumption and save electric charges due to the fluorescent lamp which is still main lighting source, we have carried out the studies on the indicating the optimal replacement time of it. For these purposes, we have evaluated the increment of power consumption of the fluorescent lamp and its economical efficiency due to degradation according to elapsed time. On the basis of the evaluation, we have designed and developed the system that turned off(or on) the red LED at the optimal replacement time. We also verified correct operations of the developed system through the circuit simulation by P-spice and experiments.

대구 유니버시아드대회 자원봉사활동에 대한 만족도 연구

황성동,성희자,고강호,전보경,권현수 慶北大學校 社會科學大學 2003 社會科學 Vol.15 No.-

佛語의 强勢後置傾向 硏究

皇甫淳 慶北大學校 1983 論文集 Vol.35 No.-

영어 교과서에 기술된 문법구조 유형 비교 연구 : 중학교 3학년 교과서를 중심으로

황보근,김정연 大邱大學校 師範大學 附設 敎育硏究所 2010 學校敎育硏究 Vol.5 No.2

The purpose of this study is to analyse the grammatical contents in middle school English textbooks for finding an effective way of grammar instruction to improve communicative ability. For this purpose, five sets of middle school English textbooks, which are authorized by the Ministry of Education according to the 7th Curriculum, were analysed. The results of this analysis are as follows : First, grammar items were presented in separate parts in three sets of textbooks and in the other textbooks, they were presented in various activities to improve communicative competence such as reading, writing, speaking, and listening skill. Second, three sets of textbooks, which grammar items were presented systematically, did not show them in situations or contexts but in simple pattern practices. So, this may lead the learners to concentrate upon mechanical learning of grammatical rules. In two sets of textbooks, grammar items were not presented systematically but, grammar learning can be made meaningful by language form being embedded in contexts. Third, the presentational method of these textbook's language forms is deductive. Currently, the inductive method, which gives students a chance to discover within a context, is preferred, and has proven to be more successful. The fundamental purpose of language is communication and, English education also aims to this. If what we said is ungrammatical, though we can speak English fluently, it's not sufficient to accurate communication. Communicative competence means the ability to speak in target language both fluently and accurately. Grammar is important, if not absolutely essential for improving communicative competence. Therefore, as one competent of communicative competence, grammar is no longer taught in isolation but in relation to reading, listening, writing in one lesson. Communicative activities suitable for a Korean classroom environment should be developed and teachers should provide students with an effective and meaningful grammar instruction. 진정한 의미의 의사소통 능력이란 정확성과 유창성을 겸하는 것이어야 한다. 따라서 언어 교육은 유 창성과 정확성 중 어느 하나만을 선택하여 획득하는 것이 아니라 둘 다를 목표로 하여야 한다. 유창성 과 정확성을 동시에 겸한 진정한 의미의 의사소통 능력을 위해서 문법 능력은 필수라는 것이 많은 연구를 통해 증명되면서 의사소통 능력 향상을 위한 효과적인 문법 교육이 영어 교육의 중요한 이슈가 되었다. 이제는 문법을 가르쳐야 하는가 아닌가 하는 문제가 아니라 의사소통 중심 영어 수업에서 문법 의 역할은 무엇인가, 좀 더 구체적으로 우리나라 교육 현실에 맞추어 어떻게 무엇을 얼마나 가르쳐야 하는가가 논의의 대상이 된 것이다. 이에 본 연구는 의사소통 능력을 향상시키기 위한 효과적인 문법 교육의 방향을 알아보기 위하여 영어 교육의 가장 기본적인 언어 자료인 영어 교과서에서 문법이 차지하는 비중과 문법 내용, 그리고 문법내용의 제시 방법에 대해 연구하고자 한다.

인터넷 기반의 전자공간을 활용한 과학기술혁신 모델

황보열,김소용 동양대학교 1999 동양대학교 논문집 Vol.5 No.1

인터넷 사용자의 폭발적인 증가와 함께 이를 기반으로 한 전자공간은 새로운 혁신을 지향하고 있다. 본 연구에서는 과학기술 혁신을 위한 전자공간 활용에 초점을 두고 국가 혁신의 새로운 모델을 도출하는 것을 목표로 하였다. 과거에는 혁신을 위한 과학기술 정보의 유통모델은 선형적인 특징을 갖고 있지만 새로운 모델은 한 곳으로 모이는 수렴(Convergence) 모델로 요약될 수 있다. 즉 새로운 과학기술 혁신 모델에서는 과학기술 정보의 공급자와 수요자가 함께 전자공간에 모여 공급과 수요가 동시에 발생하는 것이 특징이다. 결국 공급자인 동시에 수요자이고 수요자인 동시에 공급자이다. 본 연구에서는 새로운 모델을 구체화시키기 위해 정보기술을 적용한 과학기술 혁신을 위한 수요공급 아키텍쳐를 제시함과 동시에 전자공간 활용을 위한 주요 과제를 도출하였다.