Overexpression of the potato StEPF2 gene confers enhanced drought tolerance in Arabidopsis

Yanli Wang,Tian Xie,Chunli Zhang,Juanjuan Li,Zhi Wang,Hongbing Li,Xiping Liu,Li-Na Yin,Shi-Wen Wang,Sui-Qi Zhang,Xiping Deng,Qingbo Ke 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.4

Epidermal patterning factor 2 (EPF2) is a negative regulator of stomatal development, and is essential for plant growth, development, and environmental stress responses. However, the role of EPF2 in potato (Solanum tuberosum) has not been investigated to date. Here, we cloned and characterized the potato EPF2-like gene (StEPF2). StEPF2 is predominantly intercellular space localized and its transcripts were rhythmically expressed, and showed the highest expression in apical unexpanded leaves. Expression of StEPF2 was markedly down-regulated in response to abscisic acid and sodium chloride treatments; however, upon the application of polyethylene glycol, the expression of StEPF2 peaked at 4 h and then decreased gradually. Overexpression of StEPF2 in Arabidopsis (OE) substantially reduced stomatal density and photosynthetic rate, but had little effects on plant growth. Under drought stress, OE lines maintained higher photosynthetic rates, photosystem II efficiency, and instantaneous water use efficiency than wild-type (WT) plants. Moreover, OE lines showed less water loss and hydrogen peroxide accumulation in detached leaves compared with WT plants. Thus, our results suggest that StEPF2 acts as a negative regulator of stomatal development in potato, indicating that the role of EPF2 is conserved across plant species. Overall, StEPF2 represents an important target for the development of drought-tolerant potato cultivars via genetic engineering.

CO2-responsive Pickering emulsion stablized by modified silica nanoparticles: A dissipative particle dynamics simulation study

Yan Wang,Hongbing Wang,Chunling Li,Shuangqing Sun,Songqing Hu 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.97 No.-

Dissipative particle dynamics (DPD) simulations were applied to investigate the effect of SiO2nanoparticles (SNPs) modified with different tertiary amine chain length and concentration on O/Wemulsion properties, the influence mechanism was also revealed from the molecular level. On this basis,the influence of the modified SNPs volume fraction in the emulsion system was studied. The resultsshowed that with the increase number of tertiary amine chain alkyl carbon, the stability of the originalemulsion system wasfirst enhanced and then weakened while the responsiveness of the protonatedemulsion system wasfirst weakened and then slightly enhanced, the number of tertiary amine chainsmodified on SNPs has the same influence rule on emulsion performance, which was determined by thehydrophobicity of modified SNPs. In addition, with the increase of the modified SNPs volume fraction inthe emulsion system, the stability of the emulsion is gradually enhanced. However, when the volumefraction of SiO2 exceeds 3%, the amplitude of stability enhancement decreases. The increase in theinteraction energy between oil droplets in the equilibrium process is caused by emulsion fusion, which inturn is affected by SNPs. The repulsive force between oil and water is greater than that between two oildrops, which is the reason for the oil drop fusion. The presence of SNPs can reduce the repulsive forcebetween oil and water to different degrees, thus slowing down the fusion of emulsion.

Tunable stability of oil-containing foam systems with different concentrations of SDS and hydrophobic silica nanoparticles

Shuangqing Sun,Yan Wang,Congtai Yuan,Hongbing Wang,Wendong Wang,Jianhui Luo,Chunling Li,Songqing Hu 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.82 No.-

Experiment and molecular dynamics simulation were carried out to study the tunable stability of oilcontainingSDS-stabilized Nitrogen-in-water foam. The experimental results show that the foam stabilitycould be tuned by the concentrations of SDS and modified SiO2 nanoparticles. In the foam systems with alow SDS concentration (0.2 wt.%), the foams show poor stability and the foam stability was almost notaffected by the addition of modified SiO2 nanoparticles. The foam stability was greatly improved atmoderate SDS concentration (0.5 wt.%), it enhancedfirst and then weakened with the increase ofmodified SiO2 concentration, and the half-life time achieved a maximum value of 1292 s at 0.05 wt.%modified SiO2. However, at high SDS concentration (0.8 wt.%), the foam stability was pretty good exceptfor when the modified SiO2 concentration is too high (>0.2 wt.%). The microscopic mechanism wasobtained by investigating the structural and dynamic properties of the foamfilm. The simulation workshowed consistent results of foam stability with the experimental results. Moreover, it also revealed thatthe concentration and configuration of SDS will affect its interaction with SiO2 and oil molecule, which iscritical to foam stability.

Fabrication of high dense and ultra-narrow nanogaps array for flexible SERS substrate

Hongbing CAI,Yukun WU,Nan PAN,Yangchao TIAN,Yi LUO,Xiaoping WANG 한국진공학회 2016 한국진공학회 학술발표회초록집 Vol.2016 No.8

Efficient and Secure Certificateless Proxy Re-Encryption

( Ya Liu ),( Hongbing Wang ),( Chunlu Wang ) 한국인터넷정보학회 2017 KSII Transactions on Internet and Information Syst Vol.11 No.4

In this paper, we present an IND-CCA2 secure certificateless proxy re-encryption scheme in the random oracle model. A certificateless public key cryptography simplifies the certificate management in a traditional public key infrastructure and the built-in key escrow feature in an identity-based public key cryptography. Our scheme shares the merits of certificateless public key encryption cryptosystems and proxy re-encryption cryptosystems. Our certificateless proxy re-encryption scheme has several practical and useful properties - namely, multi-use, unidirectionality, non-interactivity, non-transitivity and so on. The security of our scheme bases on the standard bilinear Diffie-Hellman and the decisional Bilinear Diffie-Hellman assumptions.

Identification of Steel Pipe Defect Based on Virtual Instrument

Yin Ming,Ye Xiaohui,Wang Hongxia,Pan Hongbing 보안공학연구지원센터 2015 International Journal of Signal Processing, Image Vol.8 No.6

Aiming at the problem of traditional nondestructive testing steel pipe defect, Ultrasonic testing system based on Virtual Instrument was designed. The hardware platform is the signal source which is based on micro-controller, and the HS4 data acquisition card by USB bus. The software platform adopts the LabVIEW to program by using the multithread to acquire all kinds of signal by real-time. The nondestructive feature was found by using the filter, STFT analysis and joint time domain analysis. The designed system has been used to the pipe defect inspection of some company, which is stable and can achieve an anticipant effect.

Effects of dietary mulberry leaves on growth, production performance, gut microbiota, and immunological parameters in poultry and livestock: a systematic review and meta-analysis

Bing Geng,Jinbo Gao,Hongbing Cheng,Guang Guo,Zhaohong Wang Asian Australasian Association of Animal Productio 2024 Animal Bioscience Vol.37 No.6

Objective: This study aimed to assess the effects of dietary mulberry leaves on the growth, production performance, gut microbiota, and immunological parameters of poultry and livestock. Methods: The PubMed, Embase, and Scopus databases were systematically analyzed to identify pertinent studies up to December 2022. The effects of mulberry leaf diet was assessed using the weighted mean difference, and the 95% confidence interval was calculated using a random-effects model. Results: In total, 18 studies that sampled 2,335 poultry and livestock were selected for analysis. Mulberry leaves improved the average daily gain and reduced the feed/meat ratio in finishing pigs, and the average daily gain and average daily feed intake in chicken. In production performance, mulberry leaves lowered the half carcass weight, slaughter rate, and loin eye area in pigs, and the slaughter rate in chickens. Regarding meat quality in pigs, mulberry leaves reduced the cooked meat percentage, shear force, crude protein, and crude ash, and increased the 24 h pH and water content. In chickens, it increased the drip loss, shear force, 45 min and 24 h pH, crude protein, and crude ash. Mulberry leaves also affect the abundances of gut microbiota, including Bacteroides, Prevotella, Megamonas, Escherichia-Shigella, Butyricicoccus, unclassified Ruminococcaceae, Bifidobacterium, Lactobacillus, and Escherichia coli in poultry and livestock. Mulberry leaves at different doses were associated with changes in antioxidant capacity in chickens, and immune organ indexes in pigs. With respect to egg quality, mulberry leaves at different doses improved the shell strength, yolk color, eggshell thickness, and eggshell weight. However, moderate doses diminished the egg yolk ratio and the egg yolk moisture content. Conclusion: In general, dietary mulberry leaves improved the growth, production performance, and immunological parameters in poultry and livestock, although the effects varied at different doses.

circRNA circSnx12 confers Cisplatin chemoresistance to ovarian cancer by inhibiting ferroptosis through a miR-194-5p/SLC7A11 axis

Kaiyun Qin,Fenghua Zhang,Hongxia Wang,Na Wang,Hongbing Qiu,Xinzhuan Jia,Shan Gong,Zhengmao Zhang 생화학분자생물학회 2023 BMB Reports Vol.56 No.3

Ovarian cancer (OC) is the most common gynecological malignancyworldwide, and chemoresistance occurs in most patients,resulting in treatment failure. A better understanding of themolecular processes underlying drug resistance is crucial fordevelopment of efficient therapies to improve OC patient outcomes. Circular RNAs (circRNAs) and ferroptosis play crucialroles in tumorigenesis and resistance to chemotherapy. However,little is known about the role(s) of circRNAs in regulatingferroptosis in OC. To gain insights into cisplatin resistance inOC, we studied the ferroptosis-associated circRNA circSnx12. We evaluated circSnx12 expression in OC cell lines and tissuesthat were susceptible or resistant to cisplatin using quantitativereal-time PCR. We also conducted in vitro and in vivoassays examining the function and mechanism of lnc-LBCSs. Knockdown of circSnx12 rendered cisplatin-resistant OC cellsmore sensitive to cisplatin in vitro and in vivo by activatingferroptosis, which was at least partially abolished by downregulationof miR-194-5p. Molecular mechanics studies indicatethat circSnx12 can be a molecular sponge of miR-194-5p, whichtargets SLC7A11. According to our findings, circSnx12 amelioratescisplatin resistance by blocking ferroptosis via a miR-194-5p/SLC7A11 pathway. CircARNT2 may thus serve as an effectivetherapeutic target for overcoming cisplatin resistance in OC.

Changes in plant anthocyanin levels in response to abiotic stresses: a meta-analysis

Yan Wenjie,Li Juanjuan,Lin Xinyue,Wang Lina,Yang Xiaoxiao,Xia Xiangyu,Zhang Yuxin,Yang Shaoyu,Li Hongbing,Deng Xiping,Ke Qingbo 한국식물생명공학회 2022 Plant biotechnology reports Vol.16 No.5

Anthocyanins are small molecule antioxidants that play important roles in plant response and resistance to abiotic stresses. Their levels increase when plants are exposed to abiotic stress. However, the general response patterns and magnitude of anthocyanin increase, and how they confer resistance to abiotic stresses, are difficult to evaluate because of the influence of experimental variables. In this study, changes in plant anthocyanin content under different abiotic stresses and the effect of anthocyanin overproduction on various physiological indicators were investigated through meta-analysis derived from 1039 datasets of 102 studies. Results showed that among the different stress types, heavy metals, especially copper (Cu) and mercury (Hg), induced the most significant synthesis of anthocyanins. Among the different types of drought treatments, mannitol caused anthocyanin content in plants to increase by 2.4-fold, which was more significant than that of polyethylene glycol and natural drought. Furthermore, UV stress led plant anthocyanins to increase 1.3-fold in C4 plants, which was higher than that in C3 plants. In addition, anthocyanins increased the most when the applied stresses were low and moderate, and of short duration. Moreover, plants overexpressing genes encoding MYB transcription factors increased anthocyanin content by 5.8-fold and significantly improved plant stress tolerance. Among the 21 physiological indicators, glutathione and proline levels increased the most in transgenic plants overproducing anthocyanins when exposed to abiotic stress. Taken together, this meta-analysis indicates that anthocyanins enhance stress tolerance by improving the antioxidant, metal-chelating, and osmoregulatory abilities of the plant. The results presented here can help guide future applications of anthocyanins as plant growth regulators in dryland agriculture and breeding for plant stress tolerance.

A Novel Passivity-Based Resonant Instability Suppression Method for Grid-Connected VSC

Jiancheng Zhao,Kai Li,Xiaodong Wang,Chuan Xie,Hongbing Xu 대한전기학회 2021 Journal of Electrical Engineering & Technology Vol.16 No.1

The interaction between the three-phase voltage source converters (VSCs) and the power grid may cause high-frequency resonant instable problems. Studies have indicated that the non-passivity of VSCs is the main reason that leads to the resonances. The above-mentioned passive means that the equivalent output admittance of the VSC has a positive real part. In this paper, a novel damped Smith predictor is proposed to suppress the resonant instability problem that caused by time delay. The research proved that the proposed Smith predictor can signifi cantly improve the passivity of the VSC by reducing the width of the negative real part at the high-frequencies. Simulation and experimental results demonstrate that the proposed Smith predictor can distinctly improve the robustness of the VSC.