Damage Mechanism of Drift Ice Impact

Li Gong,Zhonghui Wang,Yaxian Li,Chunling Jin,Jing Wang 한국정보처리학회 2019 Journal of information processing systems Vol.15 No.6

The ice damage occurs frequently in cold and dry region of western China in winter ice period and spring thawperiod. In the drift ice condition, it is easy to form different extrusion force or impact force to damage tunnellining, causing project failure. The failure project could not arrive the original planning and construction goal,giving rise to the water allocation pressure which influences diversion irrigation and farming production inspring. This study conducts the theoretical study on contact-impact algorithm of drift ices crashing diversiontunnel based on the symmetric penalty function in finite element theory. ANSYS/LS-DYNA is adopted as theplatform to establish tunnel model and drift ice model. LS-DYNA SOLVER is used as the solver and LSPREPOSTis used to do post-processing, analyzing the damage degrees of drift ices on tunnel. Constructingphysical model in the experiment to verify and reveal the impact damage mechanism of drift ices on diversiontunnel. The software simulation results and the experiment results show that tunnel lining surface will formvarying degree deformation and failure when drift ices crash tunnel lining on different velocity, different plansize and different thickness of drift ice. The researches also show that there are damages of drift ice impact forceon tunnel lining in the thawing period in cold and dry region. By long time water scouring, the tunnel liningsurfaces are broken and falling off which breaks the strength and stability of the structure.

alpha-MSH prevents impairment in renal function and dysregulation of AQPs and Na-K-ATPase in rats with bilateral ureteral obstruction.

Li, Chunling,Shi, Yimin,Wang, Weidong,Sardeli, Chrysanthi,Kwon, Tae-Hwan,Thomsen, Klaus,Jonassen, Thomas,Djurhuus, Jens Christian,Knepper, Mark A,Nielsen, Soren,Frokiaer, Jorgen American Physiological Society 2006 American Journal of Physiology Vol.290 No.2

<P>The purpose of this study was to evaluate the effects of the anti-inflammatory hormone alpha-melanocyte-stimulating hormone (alpha-MSH) treatment on renal function and expression of aquaporins (AQPs) and Na-K-ATPase in the kidney in response to 24 h of bilateral ureteral obstruction (BUO) or release of BUO (BUO-R). In rats with 24-h BUO, immunoblotting revealed that downregulation of AQP2 and AQP3 was attenuated (AQP2: 38 +/- 5 vs. 13 +/- 4%; AQP3: 44 +/- 3 vs. 19 +/- 4% of sham levels; P < 0.05), whereas downregulation of Na-K-ATPase was prevented by alpha-MSH treatment (Na-K-ATPase: 94 +/- 7 vs. 35 +/- 5% of sham levels; P < 0.05). Immunocytochemistry confirmed the changes in AQP1 and Na-K-ATPase expression. Renal tubular cell apoptosis was confirmed in BUO kidneys, and alpha-MSH treatment virtually completely abolished apoptosis. Furthermore, we measured glomerular filtration rate (GFR) and effective renal plasma flow (ERPF), respectively. Forty-eight hours after BUO-R demonstrated that alpha-MSH treatment almost completely prevented the decrease in GFR (nontreated: 271 +/- 50; alpha-MSH: 706 +/- 85; sham: 841 +/- 105 microl x min(-1).100 g body wt(-1), P < 0.05) and ERPF (nontreated: 1,139 +/- 217; alpha-MSH: 2,598 +/- 129; sham: 2,633 +/- 457 microl x min(-1).100 g body wt(-1), P < 0.05). alpha-MSH treatment also partly prevented the downregulation of AQP1 and Na-K-ATPase expression in rats after BUO-R for 48 h. In conclusion, alpha-MSH treatment significantly prevents impairment in renal function and also prevents downregulation of AQP2, AQP3, and Na-K-ATPase during BUO or AQP1 and Na-K-ATPase after BUO-R, demonstrating a marked renoprotective effect of alpha-MSH treatment in conditions with urinary tract obstruction.</P>

Damage Mechanism of Drift Ice Impact

Gong, Li,Wang, Zhonghui,Li, Yaxian,Jin, Chunling,Wang, Jing Korea Information Processing Society 2019 Journal of information processing systems Vol.15 No.6

The ice damage occurs frequently in cold and dry region of western China in winter ice period and spring thaw period. In the drift ice condition, it is easy to form different extrusion force or impact force to damage tunnel lining, causing project failure. The failure project could not arrive the original planning and construction goal, giving rise to the water allocation pressure which influences diversion irrigation and farming production in spring. This study conducts the theoretical study on contact-impact algorithm of drift ices crashing diversion tunnel based on the symmetric penalty function in finite element theory. ANSYS/LS-DYNA is adopted as the platform to establish tunnel model and drift ice model. LS-DYNA SOLVER is used as the solver and LS-PREPOST is used to do post-processing, analyzing the damage degrees of drift ices on tunnel. Constructing physical model in the experiment to verify and reveal the impact damage mechanism of drift ices on diversion tunnel. The software simulation results and the experiment results show that tunnel lining surface will form varying degree deformation and failure when drift ices crash tunnel lining on different velocity, different plan size and different thickness of drift ice. The researches also show that there are damages of drift ice impact force on tunnel lining in the thawing period in cold and dry region. By long time water scouring, the tunnel lining surfaces are broken and falling off which breaks the strength and stability of the structure.

Type Drive Analysis of Urban Water Security Factors

Li Gong,Hong Wang,Chunling Jin,Lili Lu,Menghan Ma 한국정보처리학회 2020 Journal of information processing systems Vol.16 No.4

In order to effectively evaluate the urban water security, the study investigates a novel system to assess factors that impact urban water security and builds an urban water poverty evaluation index system. Based on the contribution rates of Resource, Access, Capacity, Use, and Environment, the study adopts the Water Poverty Index (WPI) model to evaluate the water poverty levels of 14 cities in Gansu during 2011–2018 and uses the least variance method to evaluate water poverty space drive types. The case study results show that the water poverty space drive types of 14 cites fall into four categories. The first category is the dual factor dominant type driven by environment and resources, which includes Lanzhou, Qingyang, Jiuquan, and Jiayuguan. The secondcategory is the threefactor dominant type driven by Access, Use, and Capability, which includes Longnan, Linxia, and Gannan. The third category is the fourfactor dominant type driven by Resource, Access, Capability, and Environment, which includes Jinchang, Pingliang, Wuwei, Baiyin, and Zhangye. The fourth category is the fivefactor dominant type, which includes Tianshui and Dingxi. The driven types impacting theurban water security factors reflected by the WPI and its model are clear and accurate. The divisions of the urban water security level supply a reliable theoretical and numerical basis for an urban water security early warning mechanism.

Long supplementary light duration under same daily light integral provided by white plus blue light-emitting diodes improves quality of greenhouse-grown tomato seedlings

Li Xin,Wang Chunling,Liu Jiawei,Guo Yu,Cheng Fei,Yang Yanjie,Yan Zhengnan 한국원예학회 2023 Horticulture, Environment, and Biotechnology Vol.64 No.6

Optimizing light conditions is critical for plant growth and seedling production in greenhouse, especially in seasons when the sunlight is insuffi cient. In the present study, tomato ( Solanum Lycopersicum cv. Fenbeibei) seedlings were grown at two levels of supplementary light duration of 6 h·d − 1 (short) (07:00–10:00 and 15:00–18:00) or 12 h·d − 1 (long) (04:00–10:00 and 15:00–21:00) provided by white light-emitting diodes (LEDs, white long and white short, WL and WS) or combinations of white and blue LEDs (white & blue long and white & blue short, WBL and WBS) with same supplementary daily light integral (at 5.5 mol m − 2 d − 1 ) after sowing, and tomato seedlings grown under sunlight only were set as the control. The per centages of light intensity provided by white and blue LEDs under the WB treatments were 85% and 15%, respectively. The results indicated that tomato seedlings grown under WBL treatment exhibited the lowest plant height and shortest hypocotyl length, which was decreased by 34.6% and 41.8%, respectively, compared with the control. The chlorophyll a, chlorophyll b, and total chlorophyll contents of tomato seedlings grown under WBL were signifi cant higher than those under other treat ments. The root fresh weight, root dry weight, and the stem fi rmness of the greenhouse-grown tomato seedlings under WBL were increased by 21.5%, 41.2%, and 38.6%, compared with those grown under WBS, respectively. The sucrose content of tomato seedlings under WBL was increased by 60.4%, compared with that under WL. In conclusion, 12 h·d − 1 supplementary light provided by combinations of white and blue LEDs led to compact morphological characteristics, superior mechanical properties, and satisfactory growth performance. Our fi ndings provide an available lighting strategy to obtain the desired morphological and quality properties of tomato seedlings grown in the seasons with insuffi cient light.

Connection the Rhizomicrobiome and Plant MAPK Gene Expression Response to Pathogenic Fusarium oxysporum in Wild and Cultivated Soybean

Chunling Chang,Shangqi Xu,Lei Tian,Shaohua Shi,Fahad Nasir,Deguo Chen,Xiujun Li,Chunjie Tian 한국식물병리학회 2019 Plant Pathology Journal Vol.35 No.6

Little known the connections between soybeans mitogen- activated protein kinase (MAPK) gene expression and the rhizomicrobiome upon invasion of the root pathogen Fusarium oxysporum. To address this lack of knowledge, we assessed the rhizomicrobiome and root transcriptome sequencing of wild and cultivated soybean during the invasion of F. oxysporum. Results indicated F. oxysporum infection enriched Bradyrhizobium spp. and Glomus spp. and induced the expression of more MAPKs in the wild soybean than cultivated soybean. MAPK gene expression was positively correlated with Pseudomonadaceae but negatively correlated with Sphingomonadaceae and Glomeraceae in both cultivated and wild soybean. Specifically, correlation profiles revealed that Pseudomonadaceae was especially correlated with the induced expression of GmMAKKK13-2 (Glyma.14G195300) and GmMAPK3-2 (Glyma.12G073000) in wild and cultivated soybean during F. oxysporum invasion. Main fungal group Glomeraceae was positively correlated with GmMAPKKK14-1 (Glyma.18G060900) and negatively correlated with GmRaf6-4 (Glyma.02G215300) in the wild soybean response to pathogen infection; while there were positive correlations between Hypocreaceae and GmMAPK3-2 (Glyma.12G073000) and between Glomeraceae and GmRaf49-3 (Glyma.06G055300) in the wild soybean response, these correlations were strongly negative in the response of cultivated soybean to F. oxysporum. Taken together, MAPKs correlated with different rhizomicrobiomes indicating the host plant modulated by the host self-immune systems in response to F. oxysporum.

Croton Tiglium Extract Induces Apoptosis via Bax/Bcl-2 Pathways in Human Lung Cancer A549 Cells

Li, Changyou,Wu, Xiao,Sun, Rongli,Zhao, Peng,Liu, Fengjuan,Zhang, Chunling Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.11

Objective: To investigate the impact of a Croton tiglium extract on cellular proliferation and apoptosis in a non-small cell lung cancer cell line (A549) in vitro. Methods: A Croton tiglium seed methanol extract was prepare and assessed for effects on A549 cells regarding cellular proliferation, apoptotic rates, and expression of apoptosis related genes and proteins using real-time PCR and immunofluorescence. Results: The tested Croton tiglium extract inhibited A549 cell proliferation in a dose- and time-dependent manner, with significant elevation of apoptotic indexes at various concentrations after 24 h. In addition, rates in both early and late stages were higher in treated than untreated groups, the $100{\mu}g/ml$ dose causing the highest levels of apoptosis. RT-PCR showed that A549 cells treated with $100{\mu}g/ml$ Croton tiglium extract for 24 h has markedly higher Bax mRNA expression levels and obviously lower Bcl-2 expression levels than controls, equivalent results being observed for proteins by immunofluorescence. However, the mRNA expression levels of Fas and caspase-8 were not significantly altered. Conclusion: A Croton tiglium extract can inhibit proliferation of A549 cells and promote apoptosis though Bax/Bcl-2 pathways.

An Evaluative Study of the Operational Safety of High-Speed Railway Stations Based on IEM-Fuzzy Comprehensive Assessment Theory

Li Wang,Chunling Jin,Chongqi Xu 한국정보처리학회 2020 Journal of information processing systems Vol.16 No.5

The general situation of system composition and safety management of high-speed railway terminal isinvestigated and a comprehensive evaluation index system of operational security is established on the basis ofrailway laws and regulations and previous research results to evaluate the operational security management ofthe high-speed railway terminal objectively and scientifically. Index weight is determined by introducinginterval eigenvalue method (IEM), which aims to reduce the dependence of judgment matrix on consistencytest and improve judgment accuracy. Operational security status of a high-speed railway terminal in northwestChina is analyzed using the traditional model of fuzzy comprehensive evaluation, and a general technique ideaand references for the operational security evaluation of the high-speed railway terminal are provided. IEM isintroduced to determine the weight of each index, overcomes shortcomings of traditional analytic hierarchyprocess (AHP) method, and improves the accuracy and scientificity of the comprehensive evaluation. Riskfactors, such as terrorist attacks, bad weather, and building fires, are intentionally avoided in the selection ofevaluation indicators due to the complexity of risk factors in the operation of high-speed railway passengerstations and limitation of the length of the paper. However, such risk factors should be considered in the followupstudies.

Connection the Rhizomicrobiome and Plant MAPK Gene Expression Response to Pathogenic Fusarium oxysporum in Wild and Cultivated Soybean

Chang, Chunling,Xu, Shangqi,Tian, Lei,Shi, Shaohua,Nasir, Fahad,Chen, Deguo,Li, Xiujun,Tian, Chunjie The Korean Society of Plant Pathology 2019 Plant Pathology Journal Vol.35 No.6

Little known the connections between soybeans mitogen-activated protein kinase (MAPK) gene expression and the rhizomicrobiome upon invasion of the root pathogen Fusarium oxysporum. To address this lack of knowledge, we assessed the rhizomicrobiome and root transcriptome sequencing of wild and cultivated soybean during the invasion of F. oxysporum. Results indicated F. oxysporum infection enriched Bradyrhizobium spp. and Glomus spp. and induced the expression of more MAPKs in the wild soybean than cultivated soybean. MAPK gene expression was positively correlated with Pseudomonadaceae but negatively correlated with Sphingomonadaceae and Glomeraceae in both cultivated and wild soybean. Specifically, correlation profiles revealed that Pseudomonadaceae was especially correlated with the induced expression of GmMAKKK13-2 (Glyma.14G195300) and GmMAPK3-2 (Glyma.12G073000) in wild and cultivated soybean during F. oxysporum invasion. Main fungal group Glomeraceae was positively correlated with GmMAPKKK14-1 (Glyma.18G060900) and negatively correlated with GmRaf6-4 (Glyma.02G215300) in the wild soybean response to pathogen infection; while there were positive correlations between Hypocreaceae and GmMAPK3-2 (Glyma.12G073000) and between Glomeraceae and GmRaf49-3 (Glyma.06G055300) in the wild soybean response, these correlations were strongly negative in the response of cultivated soybean to F. oxysporum. Taken together, MAPKs correlated with different rhizomicrobiomes indicating the host plant modulated by the host self-immune systems in response to F. oxysporum.

Therapeutic inhibition of SGK1 suppresses colorectal cancer

Xuchun Liang,Chunling Lan,Guanming Jiao,Wencheng Fu,Xuesha Long,Yu An,Kejin Wang,Jinzhe Zhou,Ting Chen,Yongqin Li,Jiahong Xu,Qi Huang,Bin Xu,Junjie Xiao 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-

Colorectal cancer (CRC) is one of the leading causes of death worldwide. Thus, the development of new therapeutic targets for CRC treatment is urgently needed. SGK1 is involved in various cellular activities, and its dysregulation can result in multiple cancers. However, little is known about its roles and associated molecular mechanisms in CRC. In present study, we found that SGK1 was highly expressed in tumor tissues compared with peri-tumor samples from CRC patients. In vitro experiments revealed that SGK1 overexpression promoted colonic tumor cell proliferation and migration and inhibited cell apoptosis induced by 5-fluorouracil (5-FU), while SGK1 shRNA and inhibitors showed the inverse effects. Using CRC xenograft mice models, we demonstrated that knockdown or therapeutic inhibition of SGK1 repressed tumor cell proliferation and tumor growth. Moreover, SGK1 inhibitors increased p27 expression and promoted p27 nuclear accumulation in colorectal cancer cells, and p27 siRNAs could attenuate the repression of CRC cell proliferation induced by SGK1 inhibitors. Collectively, SGK1 promotes colorectal cancer development via regulation