Opaque의 두께가 전장용 레진의 색채에 미치는 영향

박상원,조신석,양홍서 대한치과기재학회 1999 대한치과재료학회지 Vol.26 No.3

The purpose of this study was to evaluate the color difference of specimens with the variation of metal type and opaque thickness. Resin veneered specimens with the same color were fabricated and evaluated by the CIE L*a*b* system. Specimens were divided into 4 groups as follows ; Group P1; Resin with precious metal alloy, 0.1 mm opaque layer Group P2; Resin with precious metal alloy, 0.5 mm opaque layer Group B1; Resin with nonprecious metal alloy, 0.1 mm opaque layer Group B2; Resin with nonprecious metal alloy, 0.5 mm opaque layer The results obtained were as follows ; 1. L* value of group P1 was lower than that of group P2. There was no significant differences in a* values among tested group. 2. Opaque thickness did not produce any significant differences of L*, a*, b* value in the resin veneer groups on the base metal. 3. The ΔE*ab value between P1 and P2 was 2.228 which is noticeable, while the color difference between B1 and B2 was slight.

전장용 레진의 색안정성에 대한 연구

박상원,조신석,양홍서 대한치과기재학회 1999 대한치과재료학회지 Vol.26 No.3

In this study, metal specimens were made of semiprecious metal alloy and veneered with composite resin(Dentacolar, Kulzer). Germany, Color stability of each specimen was evaluated by the CIE L*a*b* system. Specimens were divided into 3 groups as follows ; Group 1; Resin with 0.1 mm opaque layer Group 2; Resin with 0.25 mm opaque layer Group 3; Resin with 0.5 mm opaque layer The purpose of this study was to evaluate the color difference and color stability of specimens, following to the opaque thickness. The results obtained were as follows ; 1. The group 1 had best color stability after thermocycling. 2. After thermocycling, the specimens with more than 0.25 mm opaque thickness showed detectable color change and ΔE*ab values were more than 3.3 level. 3. After thermocycling, L* value was decreased and a* value was increased in group 2 and 3.

백금-코발트 합금박막을 이용한 측온저항체 온도센서의 제작

정귀상,홍석우,박정도 東西大學校 1998 동서논문집 Vol.4 No.-

Platinum-Cobalt alloy thin films were deposited on Al₂O₃substrates by r.f. cosputtering for RTD temperature sensors. We made Pt-Co alloy resistance patterms on the Al₂O₃substrates by lift-off method and investigated the physical and electrical characteristics of these films under various conditions (the input power, working vacuum, annealing temperature, thickness of thin films) and also after annealing these films. At input power of Pt: 44 W/㎠, Co: 6.91 W/㎠, working vacuum of 10 mTorr and annealing conditions of 1000℃ and 60 min, the resistivity and sheet resistivity of Pt-Co thin films was 15 μΩ·cm and 0.5 Ω/□, respectively. The TCR value of Pt-Co alloy thin films was measured with various thickness of thin films and annealing conditions. The optimum TCR value is gained under conditions 3000 A of thin films thickness and 1000℃ of annealing temperature. These results indecate that Pt-Co alloy thin films have potentiality for the high resolution RTD temperature sensors.

Ring-on-ring 강도시험법을 이용한 200mm 반도체 실리콘 웨이퍼의 파괴강도 분석

정성민,박성은,오한석,이홍림 연세대학교 산업기술연구소 2000 논문집 Vol.32 No.2

Biaxial fracture strength of the commercial 200mm Czochralski single crystal silicon wafer was measured by the ring-on-ring technique and was compared with theoretical strength of Si single crystal. The ring-on-ring apparatus was newly designed and manufactured after analyzing stress distribution in ring-on-ring test with FEM(Finite Element Method) computerized simulation. Weibull modulus of the silicon wafer was also calculated from the measured fracture strength.

양다래 과실의 아미노산조성과 유리당의 분석

李明烈,朴貞淑,李柱烈,金忠模,洪石淳 조선대학교 기초과학연구소 1992 自然科學硏究 Vol.15 No.1

Actindia chinensis의 一般成分, 遊離糖 및 amino acid 組成狀態를 알아보기 위하여 HPLC 등으로 분석, 定量한 結果는 다음과 같다. 1. 粗蜜白質量은 2.77%, 炭水化物量은 11.97%이었다. 2. 遊離糖으로는 glucose, fructose, sucrose 및 maltose가 檢出되었으며 과육부에는 fructose, 껍질부에는 sucrose 및 maltose가 많이 함유되었다. 3. 과육질 및 껍질부에서 17種의 總 및 유리 amino acid이 검출되었는데 과육부에는 isoleucine 191.52mg/100g, glutamic acid 160.93mg/100g, 껍질부에는 g1utamic acid 203.36mg/100g, aspartic acid 168.46mg/100g으로 가장 많이 함유되어 있으며, 必須 아미노산 量은 껍질부가 528.23mg/100g으로 과육부 (350.90mg/loog)보다 약 1.5배 정도 많았다. 4. 遊離 아미노산 量은 과육부 (136.89mg/loog)가 껍질부 (120.55mg/100g)에 비해 다소 많았으며 他 과일에 비하여 histidine, arginine量이 많았다. Approximate compositions, and contents of free sugars and amino acids of edible and nonedidle portion in Kiwi fruit 〔Actindia chinensis planch〕were analyzed by HPLC. The results were as follows; The contents of protein and sugars in kiwi fruit were 2.77% and 11.97%, respectively. Glucose, fructose, sucrose and maltose were detected and fructose was rich in edible portion, and sucrose and maltose in nonedible portion. 17 kinds of total amino acids were detected in edible and nonedible portion. The contents of isoleucine(191.52mg/100g) and glutamic acid (160.93mg/100g) in edible portion, and glutamic acid (203.36mg/100g) and asparatic acid (168.48mg/100g) in nonedible portion were high. Total amounts of essential amino acids of nonedidle portion (523.23mg/100g) were rich than that of edible portion (350.90mg/100g). Free amino acids were contained 136.89mg/100g in edible portion and 120.55mg/100g in nonedible portion. Histidine and arginine were found in abundance, compared to other fruits (orange and strawbeery).

韓國 노랑 초파리의 Heterochromatin 多型現象에 관한 硏究

성기창,박흥석 성균관대학교 기초과학연구소 1987 論文集 Vol.38 No.1

Heterochromatin polymorphism in the mitotic chromosomes from Taejeon and Kang-wha of Drosophila melanogaster has been examined by using Q-banding methods. The significant difference in fluorescence banding patterns was observed only in Y-chromosome, which was arbitrarily classified into Y-type S and Y-type P. The frequency of Y-type S and Y-type P for Taejeon population was found to be 80.26% and 19.74%, while it was 88.57% and 11.43% for Kangwha population, respectively. However, no difference was observed in the heterochromatin condensing patterens at interphase. The fluorecence banding patteren observed in the 2nd, 3d, 4th, and X-chromosome from flies of both population were similar to that reported previously. The genetic and evolutionary significance of the Y-type S, and Y-type P has been discussed.

폐모세포종 : 1례보고 A Case Report

최영호,서홍주,임영혁,김정중,박성강,이석기,임진수,김은규 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.2

Pulmonary blastoma is a very rare primary neoplasm of the lung. We experienced a case of pulmonary blastoma in a 40 years old man. The mediobasal segmentectomy of the right lower lobe was performed. The patient was discharge on the postoperative days 6. There is no evidence of recurrence or metastasis on followup study during 7 months period after operation.

Calcium phosphate 결정 성장 최적화를 위한 Ti 표면의 BaTiO_3 박막 형성에 관한 연구

조진우,양홍서,박영준,황규석,송종은,이용렬 전남대학교 치과대학 2001 구강과학 Vol.13 No.2

This study was purposed to establish an optimized manufacturing process for a negatively charged ferroelectric thin flim on titanium substrate in order to develop a new implant system having the mechanical strength and bone forming ability. Ferroelectric materials show an alignment of positive and negative charges by poling treatment. Based on this property, this study was performed with the assumption that the Ca^2+ ions would be easily attracted on negatively charged surface and the attracted cation might behave as nuclei for bone-like crystal grownth in biological solutions. In order to have an osteoinducibility by the ferroelectric thin film coating, continuous and flawless crystalline thin film should be formed with ferroelectricity, and finally should be adequately poling treated. In this study, BaTiO_3 (BTO) was selected as a ferroelectric material. Before thin film coating process, most favorable poling condition was investiged with the evaluation of the difference in calcium phosphate (Ca-P) formation in simulated body fluid (SBF). Moreover, the parameters to form an optimum thin film were studied. Dense bulk BTO disks were fabricate using cold isostatic pressing(CIP) and sintered in air at 1300℃ for 2 hours. Sintered BTO disk was polished, and he crystallinity, microstructure, and ferroelectric characteristics were evaluated using an X-ray diffraction analysis (XRD), a field emission scanning electron microscope (FE-SEM), and an Impedance analyzer. To find the optimum poling condition for the calcium phosphate crystal formation, sintered bulk BTO specimens were treated with either of following poling conditions; 1) room temperature poling treatment [ polarizing field (Ep) = 25kV/cm, at 25℃ for 2 hours], 2) under Tc medium-temperaturepoling treatment (Ep=20kV/cm, from 85˚C to 25˚C for 2 hour), and 3) above Tc high-temperature poling were immesed in simulated body fluid (SBF) for 30 days, and the formation of calcium phosphate (Ca-P) layer n the surface was evaluated. The crystallinity, microstructure, and ferroelectric characteristic of BTO thin film were evaluated withthe variation of final heat treatment. It was performed after establishing the starting substance-solvent mixing ratio, coating times, and pre-heating temperature optimal to form continuous and flawless BTO thin film adequate for having ferroelectricity. Perparation of BTO thin film was made by dipping-pyrolysis technique using metal naphthenates as starting substances. BTO thin films was spin-coated on Ti substrate by dipping-pyrolysis technique using sol obtained by mixing Ba-naphthenate and Ti-naphthenate as starting substances. Pre-firing temperature of BTO thin film was performed at 450℃ because organic-solvent ws completely volatilized without any crystal growth, and the final heat treatment was performed at 600, 700 or 800℃, respectively. The results are as follows; 1. BTO disk sintered at 1300℃ for 2 hours showed average grain size of about 1㎛ and the relative dielectric constant at 1㎑ was abut 3000∼3500. 2. After immersing the sintered bulk BTO poled at respective conditions in SBF for 30 days, Ca-P layers were present on the negatively charged surfaces. In contrary, positively charged Bto surface did not show any noticeable charge of the surface microstructure after SBF immersion. 3. In he case of poling condition 1 and 2 treated with below Tc, Ca/P ratio showed a relatively low value of 1,.2∼1.5. While, in the case of poling condition 3 treated with above Tc, Ca/P ratio was 1.5∼1.67, which is similar to that of biological apatite. This phenomenon demonstrates that poling the BTO above the temperature of Tc is preferable for the Ca-P formation. 4. For thin film BTO coastin, the starting substance was prepared by an equimolar mixing of the Ba and Ti-naphthenate. Dilution of the starting substance with toluene was most appropriate at sol : toluene ratio of 5.2:4.8 Too thin sol produced porosity in film, and too thick sol resulted cracks in the film. By repeating the coating and pre-firing procedure 15 times, homogeneous film of 0.5㎛ thickness could be fabricated, and perfomed well during the poling treatment without breakdown. 5. The heat-treatmen of the BTO thin film at 700℃ produced a homogeneous crystalline structure without heterogeneous TiO2 formation. The BTO thin film heat-treated at 600℃ showed non-crystallinity and no ferroelectricity. Treatment at 800℃ produced heterogeneous TiO2 phase in the film, which is unfavorabe for having ferroelectricity by the distortion of the perovskite structure. The relative dielectic constant at 1 ㎑ was 152 and 112 by heat-treatment at 700 and 800℃, respectively. In summary, these results demonstrated that poling the ferroelectric B_aT_iO_3 surface negatively is effective for the formation of Ca-P layer in simulated body fluids. Poling condition at a temperature cooling from above Tc down to room temperature is most effective. By optimizing the method of BTO thin film coating on metal substrate for the formation of Ca-P layer, the data of this study might be applied for the manufacture of new bioactive implant system.

김치유래 젖산균의 균체지방산 분석을 이용한 분류학적 연구

이정숙,정민철,김우식,이근철,김홍중,박찬선,이헌주,주윤정,이근종,안종석,박완,박용하,민태익 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

표준균주를 포함한 230여개의 김치유래 젖산균에 대한 균체지방산(FAMEs)을 분석하였다. FAMEs profiles는 Euclidian Distance 17.5에 의해 7개의 Major Cluster와 1개의 Single Cluster로 나뉘어졌다. 이중 A, B, C 및 Cluster는 Leuconostoc속으로 분석되어졌고, F는 Lactobacillus속으로 분석되어졌다. 그리고 E와 G cluster는 두개의 Genus가 혼재되어 나타났으며 보충적인 연구가 필요하다. 앞으로 김치유래 젖산균의 균체지방산 분석결과를 기반으로 한 데이타베이스에 95가지 탄소원을 이용하는 수치분류학적 접근방법 및 Pyrolysis Mass Spectrometry 등의 화학적 분석 방법과 분자친화적 연구를 통한 종합적 분류정보 체계가 갖추어지면 젖산균의 신속, 정확한 동정 및 연구에 활발히 이용되어질 것이다. Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were sued for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined a Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacterial from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.

말초혈액으로부터 Clini-MACS®를 이용한 CD34 양성세포 분리의 안정성과 효율성

박성규,이남수,원종호,박희숙,김원석,박찬형,김현수,김효철,김형준,홍영선,김춘추,홍대식 대한조혈모세포이식학회 2002 대한조혈모세포이식학회지 Vol.7 No.1

연구배경: 최근 자가 또는 동종조혈모세포이식 분야에서 말초혈액에서 채취된 단핵구 중 CD34 양성 세포만을 선택적으로 분리하여 조혈모세포이식에 응용되고 있는데 일부 악성 림프종, 유방암, 다발성 골수종 환자에서 종양세포의 오염 가능성을 감소시키고 자가면역질환이나 동종 조혈모세포이식 중 병적으로 활성화된 T 림프구를 제거할 목적으로 사용되어 치료성적 향상에 도움이 될 것으로 기대된다. 방법: 저자들은 고용량항암요법의 적응이 되는 예후가 불량한 악성 림프종(n=14), 유방암(n=5), 다발성 골수종(n=1)을 앓고 있는 20명의 환자들을 대상으로 말초혈액으로부터 Clini-MACS®을 사용하여 선택적으로 분리한 CD34 양성세포만을 이용한 조혈모세포이식의 안정성과 효율성을 분석하고자 하였다. 분리방법의 효율성을 확인하기 위하여 CD34 양성세포 분리 전후에 단핵구 수, CD34 양성률 및 세포수를 분석하고 CD34 양성세포의 순도 및 획득률을 계산하였으며 CFU-GM 집락 배양검사와 같은 질적 분석도 병행하였다. 실제 이식이 진행된 경우는 15명이었으며 조혈모세포이식 이후 골수기능의 회복 정도를 분석하여 안정성을 확인하였다. 결과: 분리 전의 CD34 양성세포 수는 1.30×10^(8) (range 0.30~29.0)이었으며 분리 후의 CD34 양성세포 수는 0.93×10^(8) (range 0.11~10.80)이었고 CD34 양성세포의 순도는 96.0% (range 35.7~99.9%)이었으며 획득률은 70.5% (range 34.5~94.4%)이었다. 말초혈액 조혈모세포가 채취되었던 20명 중 15명이 고용량항암요법 후 조혈모세포이식이 이루어졌으며 CD34 양성세포의 수는 2.12×10^(6)/kg (range 0.44~18.1)이었다. 조혈모세포이식 후 골수기능 회복에 대한 분석에서 호중구 500/μL 이상으로 회복된 시기는 이식 후 12일째 (range 7~36일)이었고 혈소판 수가 20,000/μL 이상 유지된 시기는 15일째 (10~43일)이었다. 2명의 환자에서 3주 내에 골수정착이 이루어지지 않아 추가적인 조혈모세포 투입이 이루어졌고 그 이후 성공적인 정착이 이루어졌으며 특히, 2.0×10^(6)/kg 이상의 CD34 양성세포를 확보한 경우에서 보다 빠른 백혈구 및 혈소판 회복을 보였다(ANC>500/μL; 9 days vs 13 days, platelet>20,000/μL; 8 days vs 16 days). 320일간 추적관찰을 통하여 9명이 생존 중이며 이중 1명이 재발하여 동종조혈모세포이식을 시행하였으며 사망한 6명 교통사고로 사망한 1명 제외한 5명은 재발 후 병의 진행으로 사망하였다. 전체 생존율은 56.9%이었으며 무병생존율은 50.9%이었고 중앙 생존기간은 아직 도달하지 않았다. 결론: 말초혈액 CD34 양성세포을 이용한 조혈모세포이식은 안정적으로 이루어질 수 있으며 특히 조기 골수기능회복을 위해서는 2.0×10^(6)/kg 이상의 CD34 양성세포를 확보하여야 할 것으로 생각된다. 또한, Clini-MACS®을 이용한 CD34 양성세포의 선택적 분리는 효과적으로 이루어졌으나 향후 광범위한 연구가 필요할 것으로 생각된다. Background: The leukapheresis products of patients with breast cancer, multiple myeloma, and low grade lymphoma contain significant numbers of malignant cells. Results of previous studies suggested that graft-contaminating tumor cells can have an impact on clinical outcome. Recently, a number of devices have been developed for the positive selection of CD34+ peripheral blood progenitor cells for clinical use in autologous or allogeneic transplantation. The rationale for CD34+ selection is based on clinical studies showing a two to five log reduction of contaminating tumor cells in patients with breast cancer, multiple myeloma and low grade lymphoma. Methods: We have investigated the safety and efficacy of the CD34+ selection of peripheral blood progenitor cells for auto-transplantation. Twenty patients (14 malignant lymphomas, 5 breast cancers, 1 multiple myeloma) were mobilized using chemotherapy plus G- or GM-CSF. Results: The leukapheresis products from twenty patients with a median of 1.30×10 exp (8) (range 0.30~29.0) CD34+ cells were collected during mobilization. After Clini-MACS® procedure, the median number of CD34+ selected cells was 0.93×10 exp (8) (range 0.11~10.80) with a median recovery of 70.5% (range 34.5~94.4%) and a median purity of 96.0% (range 35.7~99.9%). Fifteen patients have been infused with CD34+ selected grafts after myeloablative preparation. The median number of reinfused CD34+ cells was 2.12×10 exp (6)/kg (range 0.44~18.1). The median time to reach an absolute neutrophil count of 500/μL and an unsupported platelet count of 20,000/μL were 12 days (range 7~36) and 15 days (range 10~43), respectively. But two patients were not reconstituted bone marrow function until 3 weeks after CD34+ cell infusion. They received un-manipulated PBPC in addition to selected CD34+ cells and then all had successive engraftment. Patients who received more than 2.0×10 exp (6) CD34+ cells/kg showed a significantly faster neutrophil and platelet recovery than patients who received less than 2.0×10 exp (6) CD34+ cells/kg (log-rank test, p<0.05). With a median follow-up of 320 days, 9 patients are alive without disease recurrence and 5 patients died of relapse. Conclusion: The selection of CD34+ cells using Clini-MACS® yield highly purified autografts, and also the engraftment ability of the progenitor and stem cells is fully retained. And auto-transplantation of CD34+ PBPC results in a rapid and stable neutrophil and platelet engraftment in patients who received an infused dose of at least 2.0×10 exp (6) CD34+ cells/kg. Further additional randomized phase III trials are required to determine whether tumor cell purging by CD34+ cell selection will have a significant impact on progression-free and overall survival in autologous transplantation.