Enhancing Removal of Acid Orange II by Heterogeneous Catalytic Ozonation Using ZnO Nanoparticles-Modified Fly Ash Composite

Hoang Van Hung,Chu Thi Hong Huyen,Nguyen Thi Dong,Nguyen Thi Hong Vien,Nguyen Thu Huyen,Luu Thi Cuc,Van Huu Tap,Nguyen Hoang,Duong Dinh Tuan,Nguyen Van Huu Luong 한국화학공학회 2024 Korean Journal of Chemical Engineering Vol.41 No.5

In this study, heterogeneous catalytic ozonation using fl y ash (FA) modifi ed ZnO nanoparticles to the decolorization of acid orange II (AOII) was studied. The combination of ZnO and fl y ash via an incipient wetness impregnation method to form ZOFA composites created a synergistic eff ect that led to effi cient catalytic ozonation activity towards AOII degradation and decolorization compared with individual ozonation processes. From the experimental results, ZOFA composite with the optimal weight ratio between ZnO NPs and FA was 20 wt% (denoted as ZOFA-20) that exhibited outstanding catalytic ozonation activity. Moreover, through other investigations including initial pH conditions and catalyst dosage, AOII could be eff ectively and optimally eliminated by 0.5 g of ZOFA-20 with an initial pH value of 11. The corresponding k value was measured as 0.248 min −1 with a maximum TOC removal content of 77.27%. Besides, ZOFA-20 could show high catalytic ozonation activity even at highly concentrated AOII concentrations while it could also retain its excellent stability and reusability over 5 continuous AOII removal cycles. From the experimental and electron paramagnetic resonance (EPR) results, hydroxyl radical ( · OH) was the main active species during the catalytic ozonation of AOII. The present study provides a promising approach to prepare novel composite-based fl y ash that would be applicable for removing azo dye and other dye pollutants in water via a catalytic ozonation strategy.

Effects of Media on the Callogenensis and Cell Mass Production in Cell Cultures of Panax vietnamensis

Luong, Hoang Van,Long, Nguyen Van,Duong, Vu Binh,Toan, Nguyen Linh,Minh, Nguyen Van,Quang, Le Bach,Kim, Nam-Hyuck,Byun, Sang-Yo The Korean Society for Biotechnology and Bioengine 2009 KSBB Journal Vol.7 No.4

This study was initiated to investigate the impacts of media types and other components on the callogenensis and cell mass production of Panax vietnamensis in the first step of the cell biomass procedure. Four media were checked: Murashige-Skoog (MS), White, Gamborg and Nitch-AII. All the four media were shown potential media for Panax vietnamensis callogenensis and cell mass production, in which the MS medium showed the best results: the successful callogenensis ratio and cell mass formation were 30% and 62,93 ${\pm}$ 3,63 mg (DW) respectively, the Nitch medium showed the lowest results: the successful callogenensis ratio and cell mass formation were 15% and 27,10 ${\pm}$ 2,24 mg (DW) respectively. The results showed that the MS medium is the most suitable medium for Panax vietnamensis callogenensis and cell mass production.

Effects of Media on the Callogenensis and Cell Mass Production in Cell Cultures of Panax vietnamensis

Hoang Van Luong,Nguyen Van Long,Vu Binh Duong,Nguyen Linh Toan,Nguyen Van Minh,Le Bach Quang,Nam Hyuck Kim,Sang Yo Byun 한국생물공학회 2009 KSBB Journal Vol.24 No.2

This study was initiated to investigate the impacts of media types and other components on the callogenensis and cell mass production of Panax vietnamensis in the first step of the cell biomass procedure. Four media were checked: Murashige-Skoog (MS), White, Gamborg and Nitch-All. All the four media were shown potential media for Panax vietnamensis callogenensis and cell mass production, in which the MS medium showed the best results: the successful callogenensis ratio and cell mass formation were 30% and 62,93 ± 3,63 mg (DW) respectively, the Nitch medium showed the lowest results: the successful callogenensis ratio and cell mass formation were 15% and 27,10 ± 2,24 mg (DW) respectively. The results showed that the MS medium is the most suitable medium for Panax vietnamensis callogenensis and cell mass production.

Access to Anti-smoking Information among School Children and its Potential Impact on Preventing Smoking Initiation: Results from the Global Youth Tobacco Use Survey (GYTS) 2014 in Viet Nam

Hoang, Van Minh,Kim, Bao Giang,Phan, Thi Hai,Trinh, Dinh Hoang,Doan, Thu Huyen,Luong, Ngoc Khue,Nguyen, Thuy Linh,Nguyen, Tuan Lam,Pham, Thi Quynh Nga Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.no.sup1

Scientific evidence on all aspects of smoking amongst youth is very important for designing appropriate interventions to reduce smoking among this vulnerable population. This paper describes current access to anti-smoking information among school children aged 13 to 15 years in Vietnam in 2014 and examines its potential impact on preventing smoking initiation. The data used in this paper were obtained from the 2014 Global Youth Tobacco Survey (GYTS) in Vietnam. Students were asked questions about their level of awareness of anti-smoking information from various sources in the past 30 days and about lessons in school regarding the dangers of tobacco use during the last 12 months. Those who have never smoked were asked "whether or not they thought about avoiding cigarettes because of health warnings on cigarette packages" and answers were analyzed in combination with data on access to anti-smoking information from other sources. The prevalence of exposure to antismoking campaigns was high among school children in Viet Nam: 55.3% of current smokers reported thoughts of smoking cessation because of health warnings on cigarette packages; 60.5% of never smokers avoided initiating smoking because of the same health warnings. The potential impact of graphic health warnings to prevent school-aged children from smoking initiation would be stronger if there was concurrent access to anti-smoking programs on the dangers of tobacco use in schools. However, school education for tobacco prevention and control has not been as strong as expected. A more comprehensive school curriculum on tobacco prevention and control is recommended to reinforce antismoking messages among school children.

Multiple Component Quantitative Analysis for the Pattern Recognition and Quality Evaluation of Kalopanacis Cortex Using HPLC

Chu Van Men,강종성,장유선,Kwan Jun Lee,이제현,TRANHONG QUANG,Nguyen Van Long,Hoang Van Luong,김영호 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.12

A quantitative and pattern recognition analyses were conducted for quality evaluation of Kalopanacis Cortex (KC) using HPLC. For quantitative analysis, four bioactive compounds, liriodendrin, pinoresinol O-β-D-glucopyranoside, acanthoside B and kalopanaxin B, were determined. The analysis method was optimized and validated using ODS column with mobile phase of methanol and aqueous phosphoric acid. The validation gave acceptable linearities (r > 0.9995), recoveries (98.4% to 101.9%) and precisions (RSD < 2.20). The limit of detection of compounds ranged from 0.4 to 0.9 μg/mL. Among the four compounds, liriodendrin was recommended as a marker compound for the quality control of KC. The pattern analysis was successfully carried out by analyzing thirty two samples from four species, and the authentic KC samples were completely discriminated from other inauthentic species by linear discriminant analysis. The results indicated that the method was suitable for the quantitative analysis of liriodendrin and the quality evaluation of KC.

Anti-inflammatory compounds from Ampelopsis cantoniensis.

Van Thu, Nguyen,Cuong, To Dao,Hung, Tran Manh,Van Luong, Hoang,Woo, Mi Hee,Choi, Jae Su,Lee, Jeong-Hyung,Kim, Jeong Ah,Min, Byung Sun Natural Product Communications 2015 Natural product communications Vol.10 No.3

<P>Many natural products have been shown to have an inhibitory effect on nitric oxide (NO), and are used as chemotherapy agents for inflammation disease. The current study was designed to evaluate the anti-inflammatory activity of chemical components from the leaves of Ampelopsis cantoniensis. Sixteen compounds (1-16) were isolated and identified. Phloretin (5) and 5,7,3',5'-tetrahydroxyflavanone (16) inhibited nitric oxide (NO) production with IC50 values of 5.2, and 18.5 μM, respectively. The inhibitory effect of compounds 5 and 16 were accompanied by dose-dependent decreases in LPS-induced nitric oxide synthase (iNOS) in RAW 264.7 cells, respectively. This study investigated the significant anti-inflammatory properties of isolated compounds from the leaves of A. cantoniensis for the first time. The findings demonstrate that A. cantoniensis could be used beneficially in the treatment of inflammation disease.</P>

Applications of Magnetite Nanoparticles for Water Treatment and for DNA and Cell Separation

Nguyen Hoang Hai,Nguyen Chau,Nguyen Hoang Luong,Nguyen Thi Van Anh,Phan Tuan Nghia 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.53 No.3

Magnetic nanoparticles with a diameter of 15 nm prepared by using the coprecipitation method have been applied to enforce the sedimentation of the solid waste, to adsorb the arsenic ions in water, to increase the DNA concentration by using a magnetic eld for the electrochemical DNA sensor and to separate the helper CD4+ T cells to determine the number of the cells in blood. A combination of magnetic nanoparticles and alum makes the solid waste in water under a magnetic field aggregate a dozen times faster than under the gravity alone. A concentration of 0.1 mg/l of arsenic in water was reduced to a value lower than the permissible concentration of 0.01 mg/l after few minutes of stirring. The particles functionalized with 3-aminopropyl triethoxysilane were used to enrich the DNA of the Herpes virus, which extended the sensitivity of an electrochemical sensor down to a concentration lower than nM/l. The particles coated with fluorescent-labeled antiCD4 antibody were used to count the helper CD4+ T cells. The fluorescence signals of the particle/cell system were two times stronger than those of the uorescence antiCD4 cell system. This can be used for the treatment of an HIV-infected patient with a simple fluorescent microscope. Magnetic nanoparticles with a diameter of 15 nm prepared by using the coprecipitation method have been applied to enforce the sedimentation of the solid waste, to adsorb the arsenic ions in water, to increase the DNA concentration by using a magnetic eld for the electrochemical DNA sensor and to separate the helper CD4+ T cells to determine the number of the cells in blood. A combination of magnetic nanoparticles and alum makes the solid waste in water under a magnetic field aggregate a dozen times faster than under the gravity alone. A concentration of 0.1 mg/l of arsenic in water was reduced to a value lower than the permissible concentration of 0.01 mg/l after few minutes of stirring. The particles functionalized with 3-aminopropyl triethoxysilane were used to enrich the DNA of the Herpes virus, which extended the sensitivity of an electrochemical sensor down to a concentration lower than nM/l. The particles coated with fluorescent-labeled antiCD4 antibody were used to count the helper CD4+ T cells. The fluorescence signals of the particle/cell system were two times stronger than those of the uorescence antiCD4 cell system. This can be used for the treatment of an HIV-infected patient with a simple fluorescent microscope.

Eudesmols Induce Apoptosis through Release of Cytochrome c in HL-60 Cells

Duc Manh Hoang,Trinh Nam Trung,Long He,Do Thi Ha,이명숙,김보연,Hoang Van Luong,안종석,배기환 한국생약학회 2010 Natural Product Sciences Vol.16 No.2

We verified that the apoptosis activities were examined by DNA fragmentation, flow cytometric analysis with annexin V staining, activation of caspase-3, and cytochrome c release. In the result, a- and b-eudesmol induced DNA fragmentation in HL-60 cells at a concentration of 80 mM, respectively. Additionally, pro-apoptotic cells sorted by flow cytometry analysis were detected in HL-60 cells to 31.77 and 29.67% with a- and b-eudesmol of 80 mM. Thus, both a- and b-eudesmol exerted caspase-3 activation and cytochrome c release at 80 mM in HL-60 cells. These results are firstly reported that the sesquiterpenes, a- and b-eudesmol are apoptosis inducers through mitochondria-dependent caspase cascade in HL-60 cells.

Screening of Protein Tyrosine Phosphatase 1B Inhibitory Activity from Some Vietnamese Medicinal Plants

Duc Manh Hoang,Trinh Nam Trung,Phan Thi Thu Hien,Do Thi Ha,Hoang Van Luong,이명숙,배기환 한국생약학회 2010 Natural Product Sciences Vol.16 No.4

Protein tyrosine phosphatase 1B (PTP1B), a negative regulator of insulin signaling, has served as a potential drug target for the treatment of type 2 diabetes. The MeOH extracts of twenty-nine medicinal plants, traditionally used in Vietnam as anti-diabetes agents, were investigated for PTP1B inhibitory activity in vitro. The results indicated that, most materials showed moderate to strong inhibitory activity with IC50 values ranging from 3.4 mg/mL to 35.1 mg/mL; meanwhile, eleven extracts (37.9%) could demonstrate PTP1B activity with IC50 values less than 15.5 mg/mL; sixteen extracts (55.2%) could demonstrate PTP1B activity with IC50 values ranging from 15.5 mg/mL to 35.1 mg/mL. The study may provide a proof, at least in a part, for the ethno-medical use in diabetes disease of these plants.

Taxus wallichiana Zucc. 현탁세포에서 Jasmonic Acid-Cellulase 복합 Elicitor가 Paclitaxel 생합성에 미치는 영향

Nguyen Ngoc Hoi,Hoang Van Luong,Nguyen Van Long,Vu Binh Duong,변상요(Sang Yo Byun) 한국생물공학회 2010 KSBB Journal Vol.25 No.2

Taxus wallichiana Zucc. 현탁세포배양에서 항암제 paclitaxel 생합성 연구를 수행하였다. 현탁세포배양에서 exponential phase의 최고 생장 속도는 0.14 day-1이었으며 배가시간이 4.96 day이었다. Paclitaxel은 배양초기에 생성되지 않고 exponential phase 말기부터 서서히 생성되어 stationary phase에서도 이어지며 declined phase에서 최고치를 나타내었다. Paclitaxel의 생산을 촉진하기 위하여 elicitor를 사용하였다. 여러 elicitor 중 jasmonic acid와 cellulase가 paclitaxel의 생성을 증가시켰다. Elicitor는 접종과 동시에 투여했을 때 가장 효과가 좋았으며, jasmonic acid와 cellulase의 combination으로 효과가 더욱 증진했는데 각각 투여할 때보다 1.8배 및 3.1배 paclitaxel 생성이 증가되었다. Cell cultures of Taxus wallichiana Zucc. were made to enhance the production of anticancer agent paclitaxel. In suspension cultures, the maximum cell growth rate in exponential growth phase was 0.14 day-1 which was correlated to 4.96 days of cell doubling time. The production of paclitaxel was non-growth associated. The paclitaxel production was started after the exponential growth phase and increased to declined phase where the maximum concentration was observed. Various elicitors were tested to enhance the production of paclitaxel. The combination of two elicitors of jasmonic acid and cellulase increased the production of paclitaxel 1.8 and 3.1 times compared to paclitaxel production by individual elicitor respectively.