20(S)-ginsenoside Rg3 exerts anti-fi brotic effect after myocardial infarction by alleviation of fi broblasts proliferation and collagen deposition through TGFBR1 signaling pathways

Honglin Xu,Haifeng Miao,Guanghong Chen,Guoyong Zhang,Yue Hua,Yuting Wu,Tong Xu,Changlei Hu,Mingjie Pang,Leyi Tan,Xin Han,Bin Liu,Yingchun Zhou 고려인삼학회 2023 Journal of Ginseng Research Vol.47 No.6

Background: Myocardial fibrosis post-myocardial infarction (MI) can induce maladaptive cardiacremodeling as well as heart failure. Although 20(S)-ginsenoside Rg3 (Rg3) has been applied to cardiovasculardiseases, its efficacy and specific molecular mechanism in myocardial fibrosis are largely unknown. Herein, we aimed to explore whether TGFBR1 signaling was involved in Rg3's anti-fibrotic effectpost-MI. Methods: Left anterior descending (LAD) coronary artery ligation-induced MI mice and TGF-b1-stimulated primary cardiac fibroblasts (CFs) were adopted. Echocardiography, hematoxlin-eosin andMasson staining, Western-blot and immunohistochemistry, CCK8 and Edu were used to study the effectsof Rg3 on myocardial fibrosis and TGFBR1 signaling. The combination mechanism of Rg3 and TGFBR1 wasexplored by surface plasmon resonance imaging (SPRi). Moreover, myocardial Tgfbr1-deficient mice andTGFBR1 adenovirus were adopted to confirm the pharmacological mechanism of Rg3. Results: In vivo experiments, Rg3 ameliorated myocardial fibrosis and hypertrophy and enhanced cardiacfunction. Rg3-TGFBR1 had the 1.78 10 7 M equilibrium dissociation constant based on SPRi analysis,and Rg3 inhibited the activation of TGFBR1/Smads signaling dose-dependently. Cardiac-specific Tgfbr1knockdown abolished Rg3's protection against myocardial fibrosis post-MI. In addition, Rg3 downregulatedthe TGF-b1-mediated CFs growth together with collagen production in vitro through TGFBR1signaling. Moreover, TGFBR1 adenovirus partially blocked the inhibitory effect of Rg3. Conclusion: Rg3 improves myocardial fibrosis and cardiac function through suppressing CFs proliferationalong with collagen deposition by inactivation of TGFBR1 pathway.

Efficient Method to Include Joint Zones of Chord Members in Finite Element Model of Tubular Transmission Tower at Linear Elastic Stage

Haifeng Liu,Junke Han,Zhenmao Sun,Jingbo Yang,Fengli Yang,Xiyu Xu 한국강구조학회 2015 International Journal of Steel Structures Vol.15 No.4

To include the effects of the joint zones efficiently, firstly a chord member considering the joint zones is seen as a combination of three beams with different sections and its element stiffness matrix is derived; Secondly, based on the statistically geometrical properties of the joint zones and chord members in service, the coefficients in the element stiffness matrix of the varied section beam element related to the moments and shear forces greatly increase more than 70%, while the coefficients related to axial forces only increase less than 2%. Therefore, along with the other two inherent mechanical properties of member structures, it is essential to include the effects of the joint zones. Finally, the results by the finite element models where the chord members and the joint zones are simulated by four methods are compared, which proves the efficiency and the accuracy of the varied section beam element.

Strategy for Fabricating Wafer-Scale Platinum Disulfide

Xu, Hongjun,Huang, Hsin-Pan,Fei, HaiFeng,Feng, Jiafeng,Fuh, Huei-Ru,Cho, Jiung,Choi, Miri,Chen, Yanhui,Zhang, Lei,Chen, Dengyun,Zhang, Duan,Coileá,in, Cormac Ó,Han, Xiufeng,Chang, Ching-Ra American Chemical Society 2019 ACS APPLIED MATERIALS & INTERFACES Vol.11 No.8

<P>PtS<SUB>2</SUB> is a newly developed group 10 2D layered material with high carrier mobility, wide band gap tunability, strongly bound excitons, symmetrical metallic and magnetic edge states, and ambient stability, making it attractive in nanoelectronic, optoelectronic, and spintronic fields. To the aim of application, a large-scale synthesis is necessary. For transition-metal dichalcogenide (TMD) compounds, a thermally assisted conversion method has been widely used to fabricate wafer-scale thin films. However, PtS<SUB>2</SUB> cannot be easily synthesized using the method, as the tetragonal PtS phase is more stable. Here, we use a specified quartz part to locally increase the vapor pressure of sulfur in a chemical vapor deposition furnace and successfully extend this method for the synthesis of PtS<SUB>2</SUB> thin films in a scalable and controllable manner. Moreover, the PtS and PtS<SUB>2</SUB> phases can be interchangeably converted through a proposed strategy. Field-effect transistor characterization and photocurrent measurements suggest that PtS<SUB>2</SUB> is an ambipolar semiconductor with a narrow band gap. Moreover, PtS<SUB>2</SUB> also shows excellent gas-sensing performance with a detection limit of ∼0.4 ppb for NO<SUB>2</SUB>. Our work presents a relatively simple way of synthesizing PtS<SUB>2</SUB> thin films and demonstrates their promise for high-performance ultrasensitive gas sensing, broadband optoelectronics, and nanoelectronics in a scalable manner. Furthermore, the proposed strategy is applicable for making other PtX<SUB>2</SUB> compounds and TMDs which are compatible with modern silicon technologies.</P> [FIG OMISSION]</BR>

Saliency-Assisted Collaborative Learning Network for Road Scene Semantic Segmentation

Haifeng Sima,Yushuang Xu,Minmin Du,Meng Gao,Jing Wang 한국인터넷정보학회 2023 KSII Transactions on Internet and Information Syst Vol.17 No.3

Semantic segmentation of road scene is the key technology of autonomous driving, and the improvement of convolutional neural network architecture promotes the improvement of model segmentation performance. The existing convolutional neural network has the simplification of learning knowledge and the complexity of the model. To address this issue, we proposed a road scene semantic segmentation algorithm based on multi-task collaborative learning. Firstly, a depthwise separable convolution atrous spatial pyramid pooling is proposed to reduce model complexity. Secondly, a collaborative learning framework is proposed involved with saliency detection, and the joint loss function is defined using homoscedastic uncertainty to meet the new learning model. Experiments are conducted on the road and nature scenes datasets. The proposed method achieves 70.94% and 64.90% mIoU on Cityscapes and PASCAL VOC 2012 datasets, respectively. Qualitatively, Compared to methods with excellent performance, the method proposed in this paper has significant advantages in the segmentation of fine targets and boundaries.

Research on Magnetic Actuation Mechanism and Kinematics of Soft Inspection Robot for HVDC Transmission Line

Xianjin Xu,Yanhao Huang,Lanlan Liu,Yu Yan,Haifeng Yan,Yuhang Yang 한국자기학회 2023 Journal of Magnetics Vol.28 No.2

Currently, most of the inspection robots for high-voltage transmission lines, both at home and abroad, utilize a multi-cantilever rigid structure. However, the inefficiency and poor safety of these robots when it comes to crossing obstacles make them impractical. To address this issue, a magnetically actuated soft inspection robot has been developed. This robot uses the amperage force applied to the current-carrying coil in a HVDC toroidal magnetic field to efficiently and flexibly cross multiple obstacles in an inchworm-like motion. The focus of this paper is on the design and theoretical calculation of the magnetically actuated model, specifically the magnetic linear traction force and magnetic adsorption force (diastolic force), required to enable the soft robot to crawl. Through simulation and kinematic analysis, the results show that the magnetically actuated soft robot design proposed in this paper is theoretically feasible, providing a foundation for future developments in magnetically actuated soft robots.

Quadratic‐wavelet‐transform‐based fault detection approach for temperature sensor

Han, Xiaojia,Xu, Aidong,Wang, Kai,Guo, Haifeng,Zhang, Ning,Liu, Yang,Hong, Seung Ho Wiley Blackwell (John Wiley Sons) 2019 IEEJ transactions on electrical and electronic eng Vol.14 No.1

Application of copper sulfate based fenton-like catalyst in degradation of quinoline

Zhaojie Jiao,Haifeng Gong,Ye Peng,Guilin Zhou,Xianming Zhang,Xu Gao,Yunqi Liu 대한환경공학회 2022 Environmental Engineering Research Vol.27 No.5

In the practical application of water treatment, the Fenton reaction usually works at a lower pH. To overcome the above shortcomings, a Fenton-like reaction system with copper sulfate as a catalyst was proposed. In this paper, quinoline was used as the target pollutant and the effects of catalyst dosage, H₂O₂ dosage, reaction temperature, and initial concentration of quinoline and pH on the removal effect were investigated, and the evolution in pH and hydroxyl radical concentration during reaction, as well as the possible catalytic mechanism and degradation pathway were clarified. The results show that under a catalyst dosage of 0.4 g・L<SUP>-1</SUP>, a H₂O₂ dosage of 196 mmol・L<SUP>-1</SUP>, a quinoline concentration of 100 mg・L<SUP>-1</SUP> and a temperature of 75°C, the removal of quinoline and total organic carbon (TOC) reaches 99.5% and 87.2% in 65 min, respectively. Furthermore, the copper sulfate-driven homogeneous Fenton system exhibits a superior adaptability to pH in the range of 3.8 to 8.8. In the degradation of quinoline, ·OH radicals may attack the nitrogen ring and the benzene ring in sequence. The work provides a technical support for the treatment of organic wastewater, and shows promising in practical applications.

Ginsenoside Rb1 ameliorates cisplatin-induced learning and memory impairments

Chen Chen,Haifeng Zhang,Hongliang Xu,Yake Zheng,Tianwen Wu 고려인삼학회 2019 Journal of Ginseng Research Vol.43 No.4

Background: Ginsenoside Rb1 (Rb1), a dominant component from the extract of Panax ginseng root,exhibits neuroprotective functions in many neurological diseases. This study was intended to investigatewhether Rb1 can attenuate cisplatin-induced memory impairments and explore the potentialmechanisms. Methods: Cisplatin was injected intraperitoneally with a dose of 5 mg/kg/wk, and Rb1 was administeredin drinking water at the dose of 2 mg/kg/d to rats for 5 consecutive wk. The novel objects recognition taskand Morris water maze were used to detect the memory of rats. Nissl staining was used to examine theneuron numbers in the hippocampus. The activities of superoxide dismutase, glutathione peroxidase,cholineacetyltransferase, acetylcholinesterase, and the levels of malondialdehyde, reactive oxygen species,acetylcholine, tumor necrosis factor-a, interleukin-1b, and interleukin-10 were measured by ELISAto assay the oxidative stress, cholinergic function, and neuroinflammation in the hippocampus. Results: Rb1 administration effectively ameliorates the memory impairments caused by cisplatin in bothnovel objects recognition task and Morris water maze task. Rb1 also attenuates the neuronal loss inducedby cisplatin in the different regions (CA1, CA3, and dentate gyrus) of the hippocampus. Meanwhile, Rb1 isable to rescue the cholinergic neuron function, inhibit the oxidative stress and neuroinflammation incisplatin-induced rat brain. Conclusion: Rb1 rescues the cisplatin-induced memory impairment via restoring the neuronal loss byreducing oxidative stress and neuroinflammation and recovering the cholinergic neuron functions.

Triptolide downregulates Rac1 and the JAK/STAT3 pathway and inhibits colitis-related colon cancer progression

Zhipeng Wang,Haifeng Jin,Ruodan Xu,Qibing Mei,Daiming Fan 생화학분자생물학회 2009 Experimental and molecular medicine Vol.41 No.10

Triptolide, a diterpenoid triepoxide from the traditional Chinese medicinal herb Tripterygium wilfordii Hook. f., is a potential treatment for autoimmune diseases as well a possible anti-tumor agent. It inhibits proliferation of coloretal cancer cells in vitro and in vivo. In this study, its ability to block progress of colitis to colon cancer, and its molecular mechanism of action are investigated. A mouse model for colitis-induced colorectal cancer was used to test the effect of triptolide on cancer progression. Treatment of mice with triptolide decreased the incidence of colon cancer formation, and increased survival rate. Moreover, triptolide decreased the incidence of tumors in nude mice inoculated with cultured colon cancer cells dose-dependently. In vitro, triptolide inhibited the proliferation, migration and colony formation of colon cancer cells. Secretion of IL6 and levels of JAK1, IL6R and phosphorylated STAT3 were all reduced by triptolide treatment. Triptolide prohibited Rac1 activity and blocked cyclin D1 and CDK4 expression, leading to G1 arrest. Triptolide interrupted the IL6R-JAK/STAT pathway that is crucial for cell proliferation, survival, and inflammation. This suggests that triptolide might be a candidate for prevention of colitis induced colon cancer because it reduces inflammation and prevents tumor formation and development. Triptolide, a diterpenoid triepoxide from the traditional Chinese medicinal herb Tripterygium wilfordii Hook. f., is a potential treatment for autoimmune diseases as well a possible anti-tumor agent. It inhibits proliferation of coloretal cancer cells in vitro and in vivo. In this study, its ability to block progress of colitis to colon cancer, and its molecular mechanism of action are investigated. A mouse model for colitis-induced colorectal cancer was used to test the effect of triptolide on cancer progression. Treatment of mice with triptolide decreased the incidence of colon cancer formation, and increased survival rate. Moreover, triptolide decreased the incidence of tumors in nude mice inoculated with cultured colon cancer cells dose-dependently. In vitro, triptolide inhibited the proliferation, migration and colony formation of colon cancer cells. Secretion of IL6 and levels of JAK1, IL6R and phosphorylated STAT3 were all reduced by triptolide treatment. Triptolide prohibited Rac1 activity and blocked cyclin D1 and CDK4 expression, leading to G1 arrest. Triptolide interrupted the IL6R-JAK/STAT pathway that is crucial for cell proliferation, survival, and inflammation. This suggests that triptolide might be a candidate for prevention of colitis induced colon cancer because it reduces inflammation and prevents tumor formation and development.

CuO-doped Ce for catalytic wet peroxide oxidation degradation of quinoline wastewater under wide pH conditions

Zhaojie Jiao,Xianming Zhang,Haifeng Gong,Donglin He,Hong Yin,Yunqi Liu,Xu Gao 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.105 No.-

This study aimed to treat toxic and refractory organic wastewater, and to address the limitations ofFenton method in treating organic wastewater, that the active components are easy to lose and usuallyoperate at the pH value of 2 3. In the present study, CuCe oxide catalyst was produced through citricacid-assisted complexation. Besides, the structural stability and catalytic wet peroxide oxidation (CWPO)performance of the catalyst after 5 times of application were delved into by characterization. The effectsof catalyst dosage and pH on quinoline removal were ascertained, while the reusability of the catalystwas explored. Moreover, the variations of pH, hydroxyl radical concentration and UV–Vis spectra inthe reaction system were analyzed. The possible degradation pathway and the catalytic mechanism werealso discussed. As suggested from the results, the catalyst exhibited high catalytic activity, structural stabilityand pH adaptability; it also had high CWPO performance for quinoline at the pH from 3.8 to 10.5,and the pH value need not be regulated. Under optimal conditions, 98.1% of quinoline and 86.1% of totalorganic carbon (TOC) were removed. In the CWPO process of quinoline, the OH attacked the nitrogen ringand the benzene ring in sequence.