<i>CYP2C19</i> haplotypes in Koreans as a marker of enzyme activity evaluated with omeprazole

Jin, S. K.,Kang, T. S.,Eom, S. O.,Kim, J.-I.,Lee, H. J.,Roh, J. Blackwell Publishing Ltd 2009 Journal of clinical pharmacy and therapeutics Vol.34 No.4

<P>Summary</P><P>Background and objective: </P><P>CYP2C19 is clinically important in Korea because of the relatively high incidence of poor metabolizers in the population. To fully understand the genetic mechanism of the <I>CYP2C19</I> defect in poor metabolizers, all variants need to be studied simultaneously. The aim of this study was to investigate the usefulness of <I>CYP2C19</I> haplotypes as a marker of CYP2C19 enzyme activity in Koreans.</P><P>Methods: </P><P>We analysed the single nucleotide polymorphisms and haplotypes of the <I>CYP2C19</I> gene in 150 healthy Koreans and found three major (frequency > 0·1) haplotypes (H1, H2 and H3). One oral dose of 40 mg omeprazole (Losec<SUP>®</SUP>) was administered to 30 subjects grouped as H1/H1, H2/H2, H1/H2, H1/H3 and H2/H3. The pharmacokinetics of omeprazole and its metabolites, 5-hydroxyomeprazole and omeprazole sulphone, in those groups was analysed.</P><P>Results and discussion: </P><P>The area under the plasma concentration–time curve (AUC<SUB>0→∞</SUB>) and elimination half-life (<I>T</I><SUB>1/2</SUB>) of omeprazole were significantly greater in the H2/H2 and H2/H3 groups than in the H1/H1 group (<I>P </I><<I> </I>0·05), whereas the metabolic ratios of omeprazole to 5-hydroxyomeprazole were also markedly higher.</P><P>Conclusion: </P><P>Although a specific SNP of <I>CYP2C19</I> may be predictive of enzyme activity, haplotyping is more reliable for identifying poor metabolizers in populations with variant alleles other than <I>CYP2C19*2</I> and <I>*3</I> alleles.</P>

Interface sulfur passivation using H₂S annealing for atomic-layer-deposited Al₂O₃ films on an ultrathin-body In_0.53Ga_0.47As-on-insulator

Jin, H.S.,Cho, Y.J.,Lee, S.M.,Kim, D.H.,Kim, D.W.,Lee, D.,Park, J.B.,Won, J.Y.,Lee, M.J.,Cho, S.H.,Hwang, C.S.,Park, T.J. New York] ; North-Holland 2014 APPLIED SURFACE SCIENCE - Vol.315 No.-

Atomic-layer-deposited Al<SUB>2</SUB>O<SUB>3</SUB> films were grown on ultrathin-body In<SUB>0.53</SUB>Ga<SUB>0.47</SUB>As substrates for III-V compound-semiconductor-based devices. Interface sulfur (S) passivation was performed with wet processing using ammonium sulfide ((NH<SUB>4</SUB>)<SUB>2</SUB>S) solution, and dry processing using post-deposition annealing (PDA) under a H<SUB>2</SUB>S atmosphere. The PDA under the H<SUB>2</SUB>S atmosphere resulted in a lower S concentration at the interface and a thicker interfacial layer than the case with (NH<SUB>4</SUB>)<SUB>2</SUB>S wet-treatment. The electrical properties of the device, including the interface property estimated through frequency dispersion in capacitance, were better for (NH<SUB>4</SUB>)<SUB>2</SUB>S wet-treatment than the PDA under a H<SUB>2</SUB>S atmosphere. They might be improved, however, by optimizing the process conditions of PDA. The PDA under a H<SUB>2</SUB>S atmosphere following (NH<SUB>4</SUB>)<SUB>2</SUB>S wet-treatment resulted in an increased S concentration at the interface, which improved the electrical properties of the devices.

Cryptotanshinone, a lipophilic compound of Salvia miltiorrriza root, inhibits TNF-α-induced expression of adhesion molecules in HUVEC and attenuates rat myocardial ischemia/reperfusion injury in vivo

Jin, Y.C.,Kim, C.W.,Kim, Y.M.,Nizamutdinova, I.T.,Ha, Y.M.,Kim, H.J.,Seo, H.G.,Son, K.H.,Jeon, S.J.,Kang, S.S.,Kim, Y.S.,Kam, S.C.,Lee, J.H.,Chang, K.C. North-Holland ; Elsevier Science Ltd 2009 european journal of pharmacology Vol.614 No.1

The aim of the present study was to evaluate the protective effect of cryptotanshinone (CTS), one of active ingredients of Salvia miltiorrhiza root, on myocardial ischemia-reperfusion injury in rat due to inhibition of some inflammatory events that occur by NF-kB-activation during ischemia and reperfusion. Myocardial ischemia and reperfusion injury was induced by occluding the left anterior descending coronary artery for 30 min followed by either 2 h (biochemical analysis) or 24 h (myocardial function and infarct size measurement) reperfusion. CTS injected (i.v.) 10 min before ischemia and reperfusion insult. CTS significantly reduced the infarct size and improved ischemia and reperfusion-induced myocardial contractile dysfunction. Furthermore, CTS inhibited NF-kB translocation, expression of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), neutrophil infiltration and MPO activity in ischemic myocardial tissues. CTS also significantly reduced plasma levels of TNF-α, IL-1β due to ischemia and reperfusion. Interestingly, H<SUB>2</SUB>O<SUB>2</SUB>-stimulated NF-kB-luciferase activity and TNF-α-induced expression of vascular cell adhesion molecule-1 (VCAM-1) and intracellular adhesion molecule-1 (ICAM-1) expressions in human umbilical vein endothelial cells (HUVEC) were significantly inhibited by CTS. Taken together, it is concluded that CTS may attenuate ischemia and reperfusion-induced microcirculatory disturbances by inhibition of proinflammatory cytokine production, reduction of neutrophil infiltration and possibly inhibition of adhesion molecules through inhibition of NF-kB-activation during ischemia and reperfusion.

UV Laser Rayleigh Scattering을 이용한 가스 성분의 농도 및 온도 분포 계측에 관한 실험적 연구

진성호(S.H.Jin),남기중(G.J.Nam),김희산(H.S.Kim),장래각(N.K.Chang),박승한(S.H.Park),깅웅(U.Kim),박경석(K.S.Park),김경수(G.S.Kim) 한국자동차공학회 1995 한국자동차공학회 춘 추계 학술대회 논문집 Vol.1995 No.11_2

Rayleigh Scattering Cross Sections(σ_i) of various gases and the temperature distributions of premixed Propane/O₂ flame are measured by high power KrF(248nm) Excimer laser and ICCD camera. Results show that σ_i of O₂ and Propane(C₃H_8) gases agree well in the 5% error range. but σ_i of H₂ has the more or less difference from the calculated value by other groups. This is attributed to the low RS signal of H₂ to noise level(SIN ratio).<br/> The temperature distributions of flame range out between 300K in the air and about 2000K in the burned area. In this temperature range, our system has the about 250K temperature resolution. Because low RS signals in the reaction area with high temperature are affected highly by noises, temperature uncertainty of this area is relatively high to another part of flame.<br/> Experimental results show that UV Rayleigh Scattering can be used for the measurement of mixing ratios of mixed gases and the temperature distributions of flame. Especially. this technique can be applied for the measurement of the mixing ratios of air/fuel before the ignition and the flame structure after the ignition inside the Engine.<br/>

Distinct clinical courses according to presenting phenotypes and their correlations to <i>ATP7B</i> mutations in a large Wilson's disease cohort

Lee, Beom H.,Kim, Joo H.,Lee, Sun Y.,Jin, Hye Y.,Kim, Kwi‐,Joo,Lee, Jin‐,Joo,Park, Jung‐,Young,Kim, Gu‐,Hwan,Choi, Jin‐,Ho,Kim, Kyung M.,Yoo, Han‐,Wook Blackwell Publishing Ltd 2011 Liver International Vol.31 No.6

<P><B>Abstract</B></P><P><B>Introduction and aims: </B> Wide phenotypic and genotypic heterogeneities in Wilson's disease (WD) have been reported, hampering the study of their correlations. The goal of this study was to identify the factors related to these diversities.</P><P><B>Methods: </B> Clinical courses and molecular genetic characteristics were analysed in 237 unrelated Korean WD families. The average follow‐up period was 8.2 ± 5.8 years.</P><P><B>Results: </B> Presenting phenotypes were classified as H1 (12.2%), H2 (42.4%), N1 (21.6%), N2 (0.4%), NX (0.4%), presymptomatic (22.4%) and other (0.4%), modifying the guidelines by Ferenci and colleagues. Age at presentation was youngest and cirrhosis was rarest in the presymptomatic group. Decompensated cirrhosis was the highest in the H1 group. Favourable outcome was rarest in the N1 group. Forty‐seven (11 novel) <I>ATP7B</I> mutations were identified in 85% of the 474 alleles. Multiplex ligation‐dependent probe amplification assays in <I>ATP7B</I> and analyses of <I>ATOX1</I> and <I>COMMD1</I> genes identified no additional mutations. Yeast complementation assays demonstrated functional perturbation of the seven novel missense mutants. Five major mutations, p.Arg778Leu, p.Ala874Val, p.Asn1270Ser, p.Lys838SerfsX35 and p.Leu1083Phe, accounted for 63% of the alleles. H1 was more common, age at presentation was younger and N1+N2+NX tended to be less common in patients with nonsense, frame shifting or splicing mutations than in those with missense mutations alone. Patients with both mutations in the transduction (Td) or the ATP hinge domain showed presymptomatic or hepatic manifestations but no neurological manifestation.</P><P><B>Conclusions: </B> The presenting phenotype strongly affects the clinical outcome of WD, and is related to the <I>ATP7B</I> mutation type and location, providing an evidence for genotype–phenotype correlations in WD.</P>

Effects of Fertilization Time and Culture Medium of Pig Oocytes Matured In Vitro by Liquid Boar Sperm Stored at 4℃

Park, C.S.,Yi, Y.J.,Kim, M.Y.,Chang, Y.J.,Lee, S.H.,Jin, D.I 충남대학교 형질전환복제돼지연구센터 2004 논문집 Vol. No.8

This study was to investigate the effects of fertilization time and culture medium of pig oocytes matured in-vitro by liquid boar sperm. The sperm rich fraction (30∼60 ml) was slowly cooled to room temperature (20∼23℃) by 2 h after collection. Semen was transferred into 15 ml tubes, centrifuged at room temperature for 10 min 800×g, and the supernatant solution was poured off. The concentrated sperm was resuspended with 5 ml of the LEN diluent to provide 1.0×10^(9) sperm/ml at room temperature. The resuspended semen was cooled in a refrigerator to 4℃. The medium used for oocyte maturation was TCM-199 supplemented with 26.19 mM sodium bicarbonate, 0.9 mM sodium pyruvate, 10μg/ml insulin, 2μg/ml vitamin B_(12), 25 mM HEPES, 10μg/ml bovine apotransferrin, 150μM cysteamine, 10IU/ml PMSG, 10 IU/ml hCG, 10 ng/ml EGF, 0.4% BSA, 75μg/ml sodium penicillin G, 50μg/ml streptomycin sulfate and 10% pFF. After about 22 h of culture, oocytes were cultured without cysteamine and hormones for 22 h at 38.5℃, 5% CO₂in air. Oocytes were inseminated with liquid boar sperm stored at 4℃ for 2 days after collection. Oocytes were coincubated for 1, 3, 6 and 9 h in 500 μl mTBM fertilization media with 1.0×10^(6) sperm/ml concentration, respectively. Thereafter, oocytes were transferred into 500 μl NCSU-23, HEPES buffered NCSU-23, PZM-3 and PZM-4 culture media, respectively, for further culture of 6, 48 and 144 h. The rates of sperm penetration and male pronuclear formation were higher in the fertilization times for 6 and 9 h than in those for 1 and 3 h. The rates of cleaved oocytes were higher in the fertilization times for 6 and 9 h (85.0 and 84.6%) than in those for 1 and 3 h (61.1 and 76.8%). The percentage of blastocyst formation from the cleaved oocytes was highest in the fertilization time for 6 h (33.6%) than in that for 1, 3 and 9 h (11.4, 23.0 and 29.6%). Mean cell numbers per blastocyst were 32.9, 27.6, 26.3 and 24.4 in the fertilization times for 6, 9, 3 and 1 h, respectively. The rate of blastocyst from the cleaved oocytes and the number of cells per blastocyst were higher in HEPES buffered NCSU-23 culture medium than in NCSU-23, PZM-3 and PZM-4 culture media. In conclusion, we found out that liquid boar sperm stored at 4℃ could be used for in-vitro fertilization of pig oocytes matured in-vitro. Also, we recommend the coincubation time of 6 h in 500 μl TBM fertilization medium with 1×10^(6) sperm/ml concentration and the HEPES buffered NCSU-23 culture medium for in-vitro fertilization of pig oocytes matured in-vitro.

이성교제 중인 대학생의 사이버섹스 중독, 외로움 및 이성관계 만족에 관한 연구

김윤정,박지야,변수영,이겨레,이아름,이지영,임재희,조혜림,진진화,최재의,박효정,이혜림 이화여자대학교 간호과학대학 2016 이화간호학회지 Vol.- No.50

Objectives: The purpose of the study was to identify the relationships between cybersex addition, loneliness and relationship satisfaction among Korean college students. Methods: 293 students were conveniently selected. The instruments used in this study were cyber-sexual addiction index, loneliness scale and relationship assesment scale. ANOVA, t-test and Pearson correlation coefficient with SPSS 22.0 Version were used to analyze the data. Results: 8.9% of the participants had moderate or severe addiction to cybersex. The level of cybersex addiction is significantly influenced by gender, period of relationship, time taking to sexual relations, contact number of cyber pornography and contact time of cyber pornography. Significant association was observed between cybersex addition, loneliness and relationship satisfaction. Conclusions: The factors mentioned in this study as well as loneliness and relationship satisfaction were important factors. Therefore, future studies on this topics need to develope interventions to reflect cultural and physical character of college students.

Trifolin induces apoptosis via extrinsic and intrinsic pathways in the NCI-H460 human non-small cell lung-cancer cell line

Kim, M.J.,Kwon, S.B.,Kim, M.S.,Jin, S.W.,Ryu, H.W.,Oh, S.R.,Yoon, D.Y. G. Fischer 2016 Phytomedicine Vol.23 No.10

<P>Background: Trifolin (kaempferol-3-O-galactoside), which is a galactose-conjugated flavonol, exhibits antifungal and anticancer effects. However, the mechanisms underlying its anticancer activities have not yet been examined. Purpose: In this study, the anticancer effects of trifolin were examined in human lung cancer cells. Methods: Cytotoxicity was determined by evaluating cell viability. Apoptosis was analyzed through flow cytometry and western blotting analysis. Death receptors and inhibitors of apoptosis were evaluated through RT-PCR. Results: Trifolin induced apoptosis in NCI-H460 human non-small cell lung cancer (NSCLC) cells by inhibiting the survival pathway and inducing the intrinsic and extrinsic apoptosis pathways. Trifolin decreased levels of Akt/p-Akt, whereas levels of expression of phosphatidylinositide 3-kinase (PI3K), cyclin D1, cyclin E, and cyclin A were not altered. Trifolin initiated cytochrome c release by inducing mitochondrial outer membrane permeabilization (MOMP). Trifolin increased Bcl-2-associated X protein (Bax) levels and decreased b-cell lymphoma 2 (Bcl-2) levels, while the levels of Bcl-xL were not altered. In addition, trifolin increased the levels of the death receptor involving the Fas/Fas ligand (FasL) and Fas-associated protein with the death domain (FADD), which consequently activated caspase-8, caspase-9, caspase-3, and the proteolytic cleavage of poly (ADP-ribose) polymerase (PARP). Conclusion: These results suggested that trifolin induced apoptosis via death receptor-dependent and mitochondria-dependent pathways and that trifolin can be used as a therapeutic agent in human lung cancer. (C) 2016 Published by Elsevier GmbH.</P>

소음성 청력장애에 관한 조사연구

김준언,이채언,문덕환,김진옥,하회영,윤병용,배기택,김용완,전종휘 인제대학교 1982 仁濟醫學 Vol.3 No.2

부산지역 일부 섬유, 고무 및 제강 산업장의 소음 작업부서 종사 근로자들중 6분법에 의한 청력장애(21dB이상)자는 조사대상의 6.30%이었다. Authors surveyed on the status of prevalence and several characters related with the occurrence of noise induced hearing impairment on 2,963 numbers of workers engaged in noisy department of twelve industries which were Textile, Rubber and Steel-Wire in Busan from July 1st, 1980 to September 30th, 1981. The obtained results were as follows: 1.About 90 percent of workers in noisy department engaged in the place of noise level less than 100dB(A). 2.The prevalence of occupational healing loss of workers engaged in noisy department by each level of hearing loss was respectively 5.43 percent at 21dB and above. 2.53 percent at 31dB and above, 0.80 percent at 41dB and above. 3.Workers of noise induced hearing impairment showed typical Cs-dip in Audiogram. 4.About 80 percent and above of workers of noise induced hearing impairment determined hearing loss of 40dB and below. 5.Hearing loss of workers of noise induced hearing impairment increased with aging. 6.Hearing loss of workers of noise induced hearing impairment was in accordance with the duration of employment in 7 years and below. 7.The occurred rate of noise induced hearing impairment using ELI(Early Loss Index) was about 92 percent(D-E scale).

농가생산 원유의 유질 및 위생진단에 관한 연구

진신흠,고문석,이종언,이현종,김은주 한국동물자원과학회 2002 한국축산학회지 Vol.44 No.1

본 시험은 제주도내 92개 낙농가중 3지역 8개 낙농가 착유우 262두에서 생산되는 우유의 품질을 분석하고, 고품질 우유생산에 저해 요인이 되는 유방염 원인균을 분석 처방하여 체세포 수가 낮은 신선한 고품질의 우유를 생산코자 시험을 수행하였다. 6∼10월 중 일일 1두당 평균 산유량은 22.4㎏, 평균 유지율은 3.69%였다. 우유내 평균 세균수는 15천/㎖로 대체로 양호 하였으며, 체세포 수는 농가 평균 254.6천/㎖ 였고, 착유우 742분방에 대한 6∼10월간 체세포수의 분포 비율은 우유 ㎖당 20만 이하 68.8%, 20∼50만 18.8%, 50만 이상 12.4%였다. 유방염 원인균별 발생분포는 포도구균성이 47분방으로 가장 많았으며, 연쇄상 구균성 17분방, 바실러스성 12분방 등으로 분석되었다. 산유량, 유지율, 세균 수, 체세포 수를 적용한 농가별 월간 조수입은 두당 평균 407천원이였으며, E와 A 농가에서는 456∼445천원으로 높은 반면, H농가는 314천원으로 낮아 농가간에 큰 소득 차이가 있었다. 결론적으로 낙농소득 향상을 위해서는 사양체계 개선에 의한 산유량 및 유지 율을 높이고, 위생적인 사양관리로 세균 수를 낮추며, 유방염의 예방 및 정기 검진에 의한 적정 처방으로 체세포 수를 경감시켜야 할 것으로 사료된다. This study was conducted to investigate the milk quality produced from dairy farms in Jeju province and to analyze the variety of pathogenes and the number of somatic cells in the milk. Data were collected from 262 lactating cows from 8 farms of three regions and the results obtained are summarized as follows, 1. The average daily milk yields and milk fat contents from June and October were 22.3 ㎏ and 3.7%, respectively. 2. The average number of bacterial counts in raw milk was 1.5×10^4/㎖, but that in farm A was over 3.0×10^4/㎖. 3. The somatic cell counts were 2.5×10^5/㎖ in average and those in farm G and H were higher than those in other farms. Their distribution in 262 lactating cows from June to October was as follows; less than 2.0×10^4 /㎖ in 68.8% of cows, 2.0-5.0×10^4/㎖ in 18.8% of cosws and more than 5.0×10^4/㎖ in 12.4% of cows. 4. Of the 113 isolates (compartments of cow udder) from mastitic milk, Staphylococcus aureus was found in 47, Streptococcus ogalatiae in 17 and Bacillus in 12. 5. The average monthly income of the farmers was 407 thousand won/head, and that in farm A or E was higher than that of farm H (456-475 thousand won vs. 314 thousand won) In conclusion, to improve income dairy farmers should reduce the somatic cell counts in the milk and mastitis infection through regular disinfection and inspection