Comparative profiling of plasma proteome from breast cancer patients reveals thrombospondin-1 and BRWD3 as serological biomarkers

Eui Jin Suh,Mohammad Humayun Kabir,Un-Beom Kang,Jong Won Lee,Jonghan Yu,노동영,이철주 생화학분자생물학회 2012 Experimental and molecular medicine Vol.44 No.1

Breast cancer is the most common cancer in women worldwide. It is necessary to identify biomarkers for early detection, to make accurate prognoses, and to monitor for any recurrence of the cancer. In order to identify potential breast cancer biomarkers, we analyzed the plasma samples of women diagnosed with breast cancer and age-matched normal healthy women by mTRAQ-based stable isotope-labeling mass spectrometry. We identified and quantified 204 proteins including thrombospondin-1 (THBS1) and bromodomain and WD repeat-containing protein 3(BRWD3) which were increased by more than 5-fold in breast cancer plasma. The plasma levels of the two proteins were evaluated by Western blot assay to confirm for their diagnostic value as serum markers. A 1.8-fold increase in BRWD3 was observed while comparing the plasma levels of breast cancer patients (n = 54) with age-matched normal healthy controls (n = 30), and the area under the receiver operating characteristic curve (AUC) was 0.917. THBS1 was detected in pooled breast cancer plasma at the ratio similar to mTRAQ ratio (>5-fold). The AUC value for THBS1 was 0.875. The increase of THBS1 was more prominent in estrogen receptor negative and progesterone receptor negative patients than receptor-positive patients. Our results are evidence of the diagnostic value of THBS1 in detecting breast cancer. Based on our findings, we suggest a proteomic method for protein identification and quantification lead to effective biomarker discovery.

Comparative profiling of plasma proteome from breast cancer patients reveals thrombospondin-1 and BRWD3 as serological biomarkers

Suh, Eui Jin,Kabir, Mohammad Humayun,Kang, Un-Beom,Lee, Jong Won,Yu, Jonghan,Noh, Dong-Young,Lee, Cheolju Korean Society for Biochemistry and Molecular Bion 2012 Experimental and molecular medicine Vol.44 No.1

Breast cancer is the most common cancer in women worldwide. It is necessary to identify biomarkers for early detection, to make accurate prognoses, and to monitor for any recurrence of the cancer. In order to identify potential breast cancer biomarkers, we analyzed the plasma samples of women diagnosed with breast cancer and age-matched normal healthy women by mTRAQ-based stable isotope-labeling mass spectrometry. We identified and quantified 204 proteins including thrombospondin-1 (THBS1) and bromodomain and WD repeat-containing protein 3 (BRWD3) which were increased by more than 5-fold in breast cancer plasma. The plasma levels of the two proteins were evaluated by Western blot assay to confirm for their diagnostic value as serum markers. A 1.8-fold increase in BRWD3 was observed while comparing the plasma levels of breast cancer patients (n = 54) with age-matched normal healthy controls (n = 30), and the area under the receiver operating characteristic curve (AUC) was 0.917. THBS1 was detected in pooled breast cancer plasma at the ratio similar to mTRAQ ratio (>5-fold). The AUC value for THBS1 was 0.875. The increase of THBS1 was more prominent in estrogen receptor negative and progesterone receptor negative patients than receptor-positive patients. Our results are evidence of the diagnostic value of THBS1 in detecting breast cancer. Based on our findings, we suggest a proteomic method for protein identification and quantification lead to effective biomarker discovery.

Genetic Distribution of Carbapenem-Resistant Enterobacteriaceae in Seoul Korea, 2018~2020

Sang-Hun Park,So-Hyun Park,Jin-Seok Kim,Jin-Kyung Yu,Jin-Kyoung Kim,Hyo-Sun Suh,Eun Young Kwon,Kyoung Ae Park,Eui Kyung Cha,Jae Min Shin,Hyo-Won Jeoung,Su-Jin Jeon,Young-Ok Hwang,Jib-Ho Lee,Yong-Seoun 대한바이러스학회 2022 Journal of Bacteriology and Virology Vol.52 No.1

Doping of donor-acceptor polymers with long side chains via solution mixing for advancing thermoelectric properties

Suh, Eui Hyun,Jeong, Yong Jin,Oh, Jong Gyu,Lee, Kyumin,Jung, Jaemin,Kang, Yong Soo,Jang, Jaeyoung Elsevier 2019 Nano energy Vol.58 No.-

<P><B>Abstract</B></P> <P>One-step doping of conjugated polymers by solution mixing is typically performed instead of sequential doping because of its simplicity. However, doped polymer solutions often exhibit poor solubility, and the presence of dopants in the produced films can disturb the molecular ordering of polymer structures. In this work, effective pairs of two donor-acceptor (D-A) type polymers and a molecular dopant characterized by high solution stability and good thermoelectric properties of the prepared thin films have been reported. The presence of long side chains in the polymer structures preserves their original solubilities and crystallinity in the solution and thin-film states, respectively, even at large amounts of added dopant (up to 38 mol%). Furthermore, the relatively shallow levels of the highest occupied molecular orbitals of the selected D-A polymers enable efficient charge transfer from the dopant species. Owing to their good charge transport properties, the doped D-A polymers exhibit outstanding thermoelectric properties with a maximum power factor of 31.5 μW m<SUP>−1</SUP> K<SUP>−2</SUP>, which is more than an order of magnitude higher than those of the control samples prepared from donor-only poly(3-hexylthiophene).</P> <P><B>Highlights</B></P> <P> <UL> <LI> Doping of two donor-acceptor polymers (PCDTFBT and PCDTPT) is performed by simple one-step solution mixing. </LI> <LI> The long side chains of the polymers allow high solution stabilities of the polymer-dopant mixtures. </LI> <LI> The polymer films retain structural coherence even after doping with high dopant ratios up to 38 mol%. </LI> <LI> Owing to their good charge transport properties, the doped polymers exhibit high thermoelectric power factors up to 31.5 μW m<SUP>-1</SUP> K<SUP>−2</SUP>. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>Outstanding thermoelectric properties of two D-A polymers are demonstrated by molecular doping with F<SUB>4</SUB>TCNQ via a simple one-step solution mixing process. The long side chains preserve good solubilities of these materials in solution and preferential molecular ordering in thin films at dopant contents of up to 38 mol%. Efficient doping is ensured by their relatively shallow HOMO levels.</P> <P>[DISPLAY OMISSION]</P>

Performance Comparison of Tracking Filters in Terms of Predicted Hitting Point

Suh, Tae-Il,Kim, Eui-Jin,Lee, Bum-Jik,Ryu, Jon-Ha,Lee, Sung-Eun 제어로봇시스템학회 2010 Transaction on control, automation and systems eng Vol.12 No.1

This article describes the performance comparison of several tracking filters from the viewpoint of gun fire control. It is common that filter performance can be represented by the RMSE of the current state. However, for the design of a gun fire control system, the performance of the tracking filter in terms of prediction is more important since the current state proceeds with a designed dynamic model to a certain future which is used for the final gun order. It is shown that the predicted results amplify the current state RMSE and can be a good measure.

A Collaboration Model in Inter-Organizations using CoP

Jin Young Jung,Dae Geun Hong,Eui Ho Suh 한국경영학회 2009 한국경영학회 통합학술발표논문집 Vol.2009 No.08

The effects of <i>Chamaecyparis obtusa</i> essential oil on pain-related behavior and expression of pro-inflammatory cytokines in carrageenan-induced arthritis in rats

Suh, Hye Rim,Chung, Hyun Joo,Park, Eui Ho,Moon, Sun Wook,Park, Su Jin,Park, Chan Woo,Kim, Yang In,Han, Hee Chul Informa UK (TaylorFrancis) 2016 Bioscience, Biotechnology, and Biochemistry Vol.80 No.1

<P>Chamaecyparis obtusa essential oil (COE) has been widely used to treat allergic diseases and was suggested to exert anti-inflammatory, antioxidant, and antimicrobial effects. This study evaluated the effects of COE on pain-related behavior and pro-inflammatory cytokines in rats with carrageenan (CGN)-induced arthritis. Reduced dynamic weight load on inflamed joint in voluntarily walking rats was used as the behavior test for arthritic pain; 10% COE-treated group was significantly attenuated pain (6-8h post-CGN injection) compared to VEH (mineral oil)-treated group. In addition, the protein levels of interleukin (IL)-1, tumor necrosis factor-, IL-6 (6-8h), and cyclooxygenase (COX)-2 (8h) within the synovial membrane, as well as IL-1, COX-2 (6-8h), and IL-6 (5-7h) within the meniscus, of 10% COE-treated group were significantly reduced. The current results implicate that COE has anti-inflammatory and anti-nociceptive effects on arthritis in rats.</P>

강원도 국민학생의 베타용혈성 연쇄구균 보균율에 관한 연구

서진태,김성권,차성호,박지영,김의종,김선주 대한감염학회 1992 감염 Vol.24 No.3

Group A β-hemolytic streptococcal pharyngitis has been studied very widely in area pediatrics, because of its severe sequelae, that is, acute rheumatic fever and acute glomerulonephritis. Throat culture is gold standard to confirm group A streptococcal (GAS) pharyngitis. Antistreptolysin-O (ASO) test is also performed to investigate the immune state of GAS pharyngitis. In this study, the carrier rate of β-hemolytic streptococci (BHS) was evaluated on 798 children of three elementary schools located in Kangweondo on March and April, 1992. The carriers of BHS were 108 (13.5%) of which male were 63 (14.1%), female 45 (12.8%) respectively. The carrier rate of BHS was high in 12 (20.3%) and 11 (19.4%) year old children, compared to 2.4% in 13 year old. The carrier rate was ranged from 13.0% to 15.8% in three schools. Of 108 strains, 79 (73.2%) were identified as group A, 14 (13.0%) as group G, 6 (5.6%) as group B, 3 (2.9%) as group C, and 6 as non-group A,B,C,G respectively. The demography of positive rate of-GAS showed similar pattern to the one of BHS. The carriers of GAS were 79 (9.9%) of which male were 49 (11.1%), female 30 (8.5%) respectively. ASO of 85 children haboring BHS were screened by Rapidia-ASO(Fujirebio, Japan). The positive rate of ASO was 79.4% in GAS cases, 65% in non-GAS cases respectively. This result means that ASO is not sufficient to differentiate true infection from carrier and can be raised in non-GAS cases. This study will help clinician know present bacteriologic and epidemiologic knowledge of BHS in Korean children.

Unasyn에 대한 임상검체에서 분리한 세균의 감수성

서진태,석종성,이삼열,정윤섭,김의종,김재식 대한감염학회 1988 감염 Vol.20 No.3

Recent clinical isolates of bacteria are frequently resistant to various antimicrobial agents including β-lactam antibiotics. Sulbactam is an β-lactamase inhibitor which can restore the activity of the β-lactam antibiotics when combined. Clinical isolates of bacteria from three university hospitals in Seoul and one in Taegu were compared for their susceptibility to sulbactam plus ampicillin (Unasyn), by the disk diffusion method. Among the penicillin G-resistant Staphylococcus aureus isolated at Kyungpuk University Hospital in Taegu and Kyung Hee University Hospital in Seoul 80.0% and 92.9% respectively were susceptible to Unasyn. Among the strains from Seoul National University Hospital, 90.7% of the penicillin G-resistant and methicillin-susceptible S. aureus and 34.3% of methicillin-resistant S. aureus were susceptible to Unasys. Among the ampicillin-resistant gram-negative bacilli isolated at Kyung Hee University Hospital 75.0% were susceptible to Unasys, while 53.6% of gentamicin-susceptible ampicillin-resistant gram-negative bacilli, and 42.5% of gentamicin-and-ampicillin resistant gram-negative bacilli were susceptible to Unasyn. Unasyn was more active against Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis and Acinetobacter anitratus than to other speices.

Forecast Driven Simulation Model for Service Quality Improvement of the Emergency Department in the Moses H. Cone Memorial Hospital

Eui H. Park,Jin Suh Park,Celestine Ntuen,Dae Beom Kim,Kendall Johnson 한국품질경영학회 2008 The Asian Journal on Quality Vol.9 No.3

Patient satisfaction with the Emergency Department(ED) in a hospital is related to the length of stay, and especially to the amount of waiting time for medical treatments. ED over-crowding decreases quality and efficiency, therefore affecting hospitals` profitability. This paper presents a forecasting and simulation model for resource management of the ED at Moses H. Cone Memorial Hospital. A linear regression forecasting model is proposed to predict the number of ED patient arrivals, and then a simulation model is provided to estimate the length of stay of ED patients, system throughput, and the utilization of resources such as triage nurses, patient beds, registered nurses, and medical doctors. The near future load level of each resource is presented using the proposed models.