담낭종양에서 CD24의 역할

임성철,이용,김동출,강길,변익건,박정희,최석민 朝鮮大學校 附設 醫學硏究所 2004 The Medical Journal of Chosun University Vol.29 No.2

CD24 is a small heavily glycosylated glycosylphosphatidylinosito-linked cell surface protein, which is expressed in hematologic malignancies as well as in a large variety of solid tumors, It appears to function as a ligand of PUselectin, an adhesion molecule that is present in activated platelets and endothelial cells. The authors aimed to evaluate CD24 protein expression in adenoma and adenocarcinoma of gallbladder to correlate to clinicopathologic data. The staining- was evaluated as stainability (negative, weak-, moderate-, strong-positive) and staining' patterns (membranous vs. intracytoplasmic) for statistical analysis. A highly significant association of cytoplasmic CD24 expression with adenocarcinoma compared to the adenoma of gallbladder was demonstrated. The present study demonstrates that CD24 was abundantly expressed on adenocarcinoma rather than on adenoma, but it was not statistically significant, Interrelation between the stainability or intracytoplasmic staining of CD24 and lymph node metastasis was not statistically significant. The authors conclude that CD24 is involved in tumorigenesis of gallbladder mucosa and intracytoplasmic expression of CD24 is an important molecular marker for malignant transformation.

가토에서 일측 요관 부분 및 완전폐색시 신장과 요관의 조직학적 변화

김상현,김용웅,육승모,한동석,노안식,임재성,김홍식,나용길,설종구 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

This study was designed to clarify histologic changes in the experimental rabbit kidney and ureter after unilateral partial or complete ureteral obstruction. A total of 20 adult white rabbits were divided into 4 subgroups in the partial obstruction followed by 2 weeks, the partial obstruction followed by 4 weeks, the complete obstruction followed by 2 weeks, and the complete obstruction followed by 4 weeks. The normal control group was consisted of 5 adult white rabbits. The kidneys and ureters for light microscopy were fixed in 10% neutral formalin, embedded in paraffin and stained with hematoxylin and eosin. Abnormal histologic fingings showed in ipsilateral kidney and ureter of all subgroups(such as dilatation of uriniferous tubule, focal necrosis and desquamation of epithelium, interstitial fibrosis, glomerular congestion, widening of Bowman's space, dilatation of ureter, epithelial cell atrophy or loss, interstitial fibrosis and interstitial inflammation). These findings were most severe in the group of the complete-obstructed ureter for 4 weeks. No significant changes showed in contralateral ureter of all subgroups. The degree of obstruction was severe and period of obstruction was long, the histological changes in ipsilateral side were severe. Despite of changes in ipsilateral side no abnormal findings showed in contralateral side. We suggest that long term experimental investigation of histologic changes in ipsilateral side and compensatory changes in contralateral side have to be studied.

復合材料 連結 部位를 갖는 엔진 마운트 設計에 關한 硏究

최원종,김권진,오인택,임동준,홍영길,황명신 한국항공대학교 1993 論文集 Vol.31 No.-

본 논문에서는 초경량 항공기의 엔진 마운트 구조물에 대한 유한요소 해석을 수행하였으며, 구조의 안전성 확보에 중요한 영향을 주는 부분인 복합재료 동체와 엔진 마운트 간의 연결 부위에 대한 해석을 위해 유한요소 프로그램을 작성하였다. 본 연구에 의해 작성된 유한요소 프로그램의 신뢰성을 확인하기 위해 실제로 시편 제작 실험을 실시하여 실험치와 해석치를 비교 검토하였다. The finite element analysis was performed on the ULM's engine mount to verify the structural safety Result showed that proposed engine mount structure has a large margine of safety. Strains and stresses in the vicinity of the joint between a composite fuselage and a metallic engine mount were calculated by the finite element analysis program. Numerical results from the model were compared with the test results. Good agreement was found between experiment and model results.

Effect of Manufacturing Method and Acidifier on the Dissolution Rate of Carvedilol from Solid Dispersion Formulations

Lim, Dong-Kyun,Bae, Jeong-Woo,Song, Byung-Joo,Jo, Han-Su,Kim, Hyoung-Eun,Lee, Dong-Won,Khang, Gil-Son The Korean Society of Pharmaceutical Sciences and 2011 Journal of Pharmaceutical Investigation Vol.41 No.6

In this study, we demonstrated the release behavior of carvedilol with the content of polyvinylpyrrolidone K-30 (PVP K-30) and the effect of citric acid and fumaric acid as acidifiers on the release behavior of drug. In addition, it tries to inquire into the release behavior difference of the carvedilol according to the manufacturing method. The release behavior of the tablets was compared with Dilatrand$^{(R)}$ in the simulated gastric fluid (pH1.2). Differential scanning calorimeter (DSC), X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FT-IR) were characterized for the physicochemical properties of the tablets. In case of mixing the carvedilol and PVP K-30, in case the ratio of the carvedilol and PVP K-30 was 1:5, the release behavior was the highest among. As well as the dissolution rate of tablets manufactured by lyophilization and rotary evaporator was higher than physical mixture. The dissolution rate of containing acidifiers was more improved. But, rather the excessive amount of the acidifier addition reduced the dissolution rate.

ELSEVIER : Ubiquitination-dependent CARM1 degradation facilitates Notch1-mediated podocyte apoptosis in diabetic nephropathy

( Dong Gil Kim ),( Seulki Lim ),( Min Jung Park ),( Joo Hee Choi ),( Jong Choon Kim ),( Ho Jae Han ),( Kyung Chul Yoon ),( Kwon Seop Kim ),( Jae Hyang Lim ),( Soo Hyun Park ) 전남대학교 약품개발연구소 2014 약품개발연구지 Vol.23 No.-

Podocyte apoptosis induced by hyperglycemia is considered a critical factor in the development of diabetic nephropathy. Recent studies have implicated Notch signaling in podocyte apoptosis; however, its regulatory mechanisms are not fully understood. In this study. we found that high-glucose treatment increased Notchl and jagged-l expression, the transcriptional activity of Hes. and podocyte apoptosis, and decreased the expression of coactivator-associated arginine methyltransferase 1 (CARMI) in rat podocytes. Transient transfection of CARMI reversed high-glucose-induced Notchl expression. the transcriptional activity of Hes, and podocyte apoptosis. Moreover. the silendng of CARMI using siRNA increased Notchl expression. the transctiptional activity of Hes, and podocyte apoptosis. However, the Glu266-mediated enzymatic activity of CARMI was not necessary for Notch signaling activation and podocyte apoptosis. Here, we demonstrate that AMP-activated protein kinase alpha (AMPKa) and cannabinoid receptor 1 (CB,R) are regulated by CARMI and that high-glucose-induced podocyte apoptosis is mediated by a CARMl-AMPKa-Notchl-CB,R signaling axis. We also show that highglucose-induced CARMI downregulation is due to ubiquitination-dependent CARMI degradation. Finally, we demonstrate that CARMI expression in podocytes was diminished in rats with streptozotocin-induced diabetes compared to vehicle-treated rats. Together, our data provide evidence that ubiquitination-dependent CARMI degradation in podocytes in diabetes promotes podocyte apoptosis via Notch 1 activation. Strategies to preserve CARMI expression or reduce the enzymatic activity of a ubiquitin ligase specific for CARMI could be used to prevent podocyte loss in diabetic nephropathy. ⓒ2014 Published by Elsevier lnc.

Potential hybrids of Miscanthus sinensis x M. sacchariflorus revealed by morphological traits analysis

Soo-Hyun Lim,Hae-Rim Park,Dong-Gil Kim,DoKyoung Lee,Gyoungju Nah,Do-Soon Kim 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

More than 300 Miscanthus accessions as a potential bioenergy crop were collected in Korea and their morphological traits were investigated at various growth stages. Among morphological traits, stem growth habit, the presence of awn in spikelet, and autumn new shoot are the most important key traits enabling to cluster Miscanthus accessions into M. sinensis and M. sacchariflorus groups. Miscanthus sinensis has bunch stem growth habit and awn in spikelet, and produces autumn new shoot, while M. sacchariflorus has scattering stem growth habit with no awn in spikelet and does not produce autumn new shoot. Interestingly, we found several Miscanthus accessions showing intermediate morphological traits. 7 M. sinensis accessions showed morphological traits similar to M. sacchariflorus and 17 M. sacchariflorus accessions showed morphological traits similar to M. sinensis. Flow cytometry and chromosome counting finally revealed 5 Miscanthus hybrids, suggesting that they are resulted from natural hybridization between M. sinensis and M. sacchariflorus. Therefore, these Miscanthus hybrids can be used to understand genetic recombination between these two Miscanthus species and our understanding may support future efforts for breeding new Miscanthus variety with high biomass productivity and environmental adaptability.

Vein of Galen 기형 환아에서 중재적 방사선하 혈관내 색전술시의 마취관리 경험

박성식,임동건,한규석,홍정길,김용선 대한마취과학회 2001 Korean Journal of Anesthesiology Vol.41 No.3

Anesthetic Management during Interventional Neuroradiologic Embolization of a Vein of Galen Malformation - A case report - Kyu Seok Han, M.D., Sung Sik Park, M.D., Dong Gun Lim, M.D. Yong Sun Kim, M.D.^*, and Jung Gil Hong, M.D. Department of Anesthesiology and ^*Diagnostic Radiology, School of Medicine, Kyungpook National University, Daegu, Korea The vein of Galen malformation is a midline intracranial arteriovenous fistula with aneurysmal dilatation of the vein of Galen. This abnormality, though rare, is associated with a bleak prognosis and significant mortality. However, significant advancements have been made in understanding the path-ophysiology of this abnormality, and improvements in both interventional neuroradiologic embolization and microneurosurgical techniques have contributed to a better outcome. In neuroradiologic embolization, children are likely to become agitated and confused and thereby jeopardize the entire procedure. For this reason, this procedure is performed with the children under general anesthesia. We experienced a case of neuroradiologic embolization under general anesthesia with propofol. The patient was 9-year-old boy who weighed 29 kg. He was healthy and had no other abnormalities in our preoperative evaluation. Throughout the entire procedure, especially before and after closure of the fistula, hemo-dynamic changes and unexpected events did not appear. (Korean J Anesthesiol 2001; 41: 388~392)

The Safety Evaluation of a Potent Angiogenic Activator, Synthetic Peptide (SFKLRY-NH₂) for the Skin Application

Dong Ha Kim,Yun Young Lim,Hyeong Mi Kim,So Young Kim,Beom Joon Kim,Sung-Gil Park,Taehoon Lee,Soo-Muk Cho 한국독성학회 2012 Toxicological Research Vol.28 No.1

A novel synthetic hexapeptide (SFKLRY-NH₂) that displays angiogenic activity has been identified by positional scanning of a synthetic peptide combinatorial library (PS-SPCL). This study was carried out to investigate the irritation of the SFKLRY-NH₂ on the skin. The tests were performed on the basis of Korea Food and Drug Administration (KFDA) guidelines. In results, cell toxicity is not appeared for SFKLRYNH₂ in HaCaT cells and B16F10 cells. SFKLRY-NH₂ induced no skin irritation at low concentration (10 μM), mild irritation at high concentration (10mM). We consider that this result is helpful for saying about the safety of SFKLRY-NH₂ in clinical use.

Parametric study of propeller boss cap fins for container ships

Lim, Sang-Seop,Kim, Tae-Won,Lee, Dong-Myung,Kang, Chung-Gil,Kim, Soo-Young The Society of Naval Architects of Korea 2014 International Journal of Naval Architecture and Oc Vol.6 No.2

The global price of oil, which is both finite and limited in quantity, has been rising steadily because of the increasing requirements for energy in both developing and developed countries. Furthermore, regulations have been strengthened across all industries to address global warming. Many studies of hull resistance, propulsion and operation of ships have been performed to reduce fuel consumption and emissions. This study examined the design parameters of the propeller boss cap fin (PBCF) and hub cap for 6,000TEU container ships to improve the propulsion efficiency. The design parameters of PBCF have been selected based on the geometrical shape. Computational fluid dynamics (CFD) analysis with a propeller open water (POW) test was performed to check the validity of CFD analysis. The design of experiment (DOE) case was selected as a full factorial design, and the experiment was analyzed by POW and CFD analysis. Analysis of variance (ANOVA) was performed to determine the correlation among design parameters. Four design alternatives of PBCF were selected from the DOE. The shape of a propeller hub cap was selected as a divergent shape, and the divergent angle was determined by the DOE. Four design alternatives of PBCF were attached to the divergent hub cap, and the POW was estimated by CFD. As a result, the divergent hub cap with PBCF has a negative effect on the POW, which is induced by an increase in torque coefficient. A POW test and cavitation test were performed with a divergent hub cap with PBCF to verify the CFD result. The POW test result showed that the open water efficiency was increased approximately 2% with a divergent hub cap compared to a normal cap. The POW test result was similar to the CFD result, and the divergent hub cap with the PBCF models showed lower open water efficiency. This was attributed to an increase in the torque coefficient just like the CFD results. A cavitation test was performed using the 2 models selected. The test result showed that the hub vortex is increased downstream of the propeller.

RELIABILITY ANALYSIS OF GRAVITY-BASED OFFSHORE WIND TURBINE FOUNDATION CONSIDERING LOAD UNCERTAINTY

Dong Hyawn Kim,Sang Geun Lee,Gil Lim Yoon 한국신재생에너지학회 2014 AFORE Vol.2014 No.-