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최순규,엄기수,성현순,김윤영,이시우,정대일,이용균 동아대학교 환경문제연구소 1994 硏究報告 Vol.17 No.2
The absorption ability of low density heavy metal of pine needles and cones is better than one of existing absorption materials in treatment ability and experimental condition. The elimination ability of chlorine of pine needles and cones is better than one of active carbon. In elimination of trihalomethane, the effect of treatment of pine needles is lower than one of pine cones. And surface of pine needles and cones after absorption of heavy metal ion was observed by Scanning Electron Microscope(SEM). Application and availability of pine needles and cones as absorption material need lots of experiments. The experiment on Gal-Bi(fallen leaves) is proceeding.
Bing Chun Yan,In Hye Kim,Joon Ha Park,Ji Hyeon Ahn,Jeong-Hwi Cho,Bai Hui Chen,Jae-Chul Lee,Jung Hoon Choi,Ki-Yeon Yoo,Choong Hyun Lee,Jun Hwi Cho,Jong-Dai Kim,Moo-Ho Won 한국실험동물학회 2013 Laboratory Animal Research Vol.29 No.3
In the present study, we investigated the effect of Tetaus toxin (TeT) on cell proliferation and neuroblast differentiation using specific markers: 5-bromo-2-deoxyuridine (BrdU) as an exogenous marker for cell proliferation, Ki-67 as an endogenous marker for cell proliferation and doublecortin (DCX) as a marker for neuroblasts in the mouse hippocampal dentate gyrus (DG) after TeT treatment. Mice were intraperitoneally administered 2.5 and 10 ng/kg TeT and sacrificed 15 days after the treatment. In both the TeT-treated groups, no neuronal death occurred in any layers of the DG using neuronal nuclei (NeuN, a neuron nuclei maker) and Fluoro-Jade B (F-J B, a high-affinity fluorescent marker for the localization of neuronal degeneration). In addition, no significant change in glial activation in both the 2.5 and 10 ng/kg TeT-treated-groups was found by GFAP (a marker for astrocytes) and Iba-1 (a marker for microglia) immunohistochemistry. However, in the 2.5 ng/kg TeT-treated-group, the mean number of BrdU, Ki-67 and DCX immunoreactive cells, respectively, were apparently decreased compared to the control group, and the mean number of each in the 10 ng/kg TeT-treated-group was much more decreased. In addition, processes of DCX-immunoreactive cells, which projected into the molecular layer, were short compared to those in the control group. In brief, our present results show that low dosage (10 ng/kg) TeT treatment apparently decreased cell proliferation and neuroblast differentiation in the mouse hippocampal DG without distinct gliosis as well as any loss of adult neurons.
Single and Competitive Isotherms of Phenol and o - Cresol by Pulsed - Input Method
Choi, Yong Seok,Lee, Ju Weon,Koo, Yoon Mo,Row, Kyung Ho,Choi, Dai Ki 한국화학공학회 2000 Korean Journal of Chemical Engineering Vol.17 No.6
There is a considerable industrial interest in both the scale-up and optimization of chromatographic operations used in the purification of fine chemicals and biomolecules. One of the major factors affecting the adsorption operation is the adsorption isotherm. Reversed-phase high-performance liquid chromatography (RP-HPLC) was used to measure the adsorption isotherm of phenol and o-cresol. From the experimental results, the retention times were decreased with increasing sample sizes, and the front of the peak was very stiff, so Langmuir adsorption isotherm was applied. Also Early-eluting component, phenol, in a mixture of the two components elutes faster than that in a pure component. Pulsed-injection method (PIM) was used to determine the two parameters of the Langmuir isotherm. The resulting parameters, a and b, were used to calculate the elution profiles of phenol and o-cresol in pure and mixed state. The agreement between the experimental data and calculated elution profiles was fairly good in a mixture as well as a pure component.
The Efficacy and Safety of Clopidogrel Resinate as A Novel Polymeric Salt Form of Clopidogrel
Ki, Min-Hyo,Choi, Mee-Hwa,Ahn, Kwang-Bok,Kim, Byoung-Su,Im, Dai-Sig,Ahn, Soon-Kil,Shin, Hee-jong 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.2
A novel polymeric salt of clopidogrel, clopidogrel resinate, was prepared as a anticoagulant drug. To prove the feasibility as a new active substance, clopidogrel resinate was evaluated for its efficacy and safety. In accelerated stability tests, the clopidogrel resinate tablet $(Pregrel^{(R)})$ showed less brown discoloration and fewer impurities than the clopidogrel bisulfate tablets under open and closed conditions. In toxicity tests, no deaths occurred after a single dose of up to 2,000 mg/kg/day and 13-week repeated doses of up to 625 mg/kg/day in rats without abnormal symptoms compared to clopidogrel bisulfate. When clopidogrel resinate was treated onto Caco-2 cell monolayers, clopidogrel, but not the resin, permeated across the cells with a hight permeation coefficient $(P_{app})$ of $13.5{\pm}1.13{\times}10^{-6}$ cm/sec. Clopidogrel resinate and clopidogrel bisulfate showed similar pharmacokinetics following oral administration to beagle dogs. A single oral administration of clopidogrel resinate dose-dependently inhibited ADP-induced ex vivo aggregation up to 30 mg/kg in rats. In conclusion, clopidogrel resinate was proved to be an efficient and safe polymeric salt as a candidate for a new clopidogrel salt.
Choi, Yun-Hyuk,Yang, Ki Dong,Kim, Dai-Hong,Nam, Ki Tae,Hong, Seong-Hyeon Elsevier 2017 Materials letters Vol.188 No.-
<P><B>Abstract</B></P> <P>The flux-mediated one-pot solution process based on the metal-organic decomposition (MOD) has been developed for the preparation of p-type complex oxide semiconductor CuBi<SUB>2</SUB>O<SUB>4</SUB> thin films. The precursor solutions with the various concentrations (0.5, 2, 5, and 10mol% vs. CuBi<SUB>2</SUB>O<SUB>4</SUB>) of aliovalent Li(I) flux produced the continuous and dense CuBi<SUB>2</SUB>O<SUB>4</SUB> thin films with stoichiometric composition by facilitating the crystallization from the liquid precursor solution. These films showed the high hole density and charge transfer characteristics, which lead to the high photoelectrochemical activity for water reduction.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The flux-mediated one-pot solution process for CuBi<SUB>2</SUB>O<SUB>4</SUB> thin films is developed. </LI> <LI> The dense and continuous CuBi<SUB>2</SUB>O<SUB>4</SUB> film is obtained with the aliovalent Li(I) flux. </LI> <LI> CuBi<SUB>2</SUB>O<SUB>4</SUB> films exhibit high photoelectrochemical activity for water reduction. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Ki-Yeon Yoo,Hwa Li,Ok Kyu Park,Choong Hyun Lee,Jung Hoon Choi,Moo-Ho Won,Tongkun Pai,In Koo Hwang,Jong Dai Kim 한국실험동물학회 2009 Laboratory Animal Research Vol.25 No.2
Diospyros kaki (Persimmon) fruits and leaves have been used as a traditional medicine and include a number of therapeutic components. The present study investigated neuroprotective effect of the extract of Diospyros kaki stem (EDKS) against ischemic damage in the gerbil hippocampal CA1 region after 5 min of transient cerebral ischemia. To elucidate the neuroprotective effect of EDKS on ischemic damage, 50 mg/kg EDKS was administered orally to gerbils for 7 days before the ischemic surgery, and we used cresyl violet and Fluoro-Jade C (F-J C) staining. In the EDKS-treated group, many survived pyramidal neurons in the CA1 region was stained with cresyl violet and a few degenerative pyramidal neurons was stained with F-J C. The survival rate of the CA1 pyramidal neurons was about 62% compared to that in the vehicletreated group. In the EDKS-treated group, isolectin B4 (a marker for activated microglia) immunoreactivity was significantly lower than that in the vehicle-treated group. These results suggest that repeated supplement of EKDS can protect neurons from ischemic damage.
배양 흰쥐 인슐린종세포에서 싸이클로스포린에 의한 세포독성에 미치는 글루타치온의 영향
최동헌,이병래,장대용,김종오,김병수,정기영,임태영,신병철,배학연 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.26 No.1
연구배경:Cycloporine A는 장기이식 환자에게 면역거부 반응을 억제할 목적으로 사용되고 있는데 이는 당뇨병, 고혈압, 신장세포 손상 등 여러 가지 부작용을 초래한다. Cyclosporine A의 이런 부작용에 대한 독성 기전은 아직 명확치 않으나 산화적 세포손상이 한 요인으로 생각되고 있다. 본 실험에서는 Cyclosprine A에 의한 췌장 베타세포의 독성이 미치는 GSH의 영향을 알아 보았다. 방법:흰쥐 인슐린종(RIN5mF)세포를 배양한 후 배양액에 글루타치온 합성효소 억제제인 buthione sulfoximine(BSO)과 cyclosporine A(CsA)및 GSH을 첨가하여 배양한 후 생존한 세포를 MTT법으로 측정하였다. 결과:CsA 용량이 증가함에 따라서 흰쥐 인슐린종 세포의 생존율은 감소되었다. BSO+CsA 투여군의 MTT 환원율은 CsA 단독 투여군보다 감소되었다. GSH+CsA 투여군의 MTT 환원율은 CsA 단독 투여군보다 증가되고, GSH+CsA+BSO 투여군의 MTT 환원율은 CsA+BSO 투여군보다 증가되었다. 결론:CsA는 췌장 베타세포에 직접적인 손상을 유발할 수 있으며, GSH은 CsA에 의한 베타손상방지 기능이 있는 것으로 추측된다. Background : Cyclosporin A (CsA) is a immunosuppressive agent that is most widely used in organ transplanted patients to prevent immunorejection, However, it has some side effects, including diabetes mellitus, nephrotoxicity and hypertension. The mechanism of CsA cytotoxicity is unclear but it has been suggested that reactive oxygen species are involved in the cytotoxic reactions. The purpose of this study was to determine the effects of glutathione, as a physiological antioxidant on CsA induced β-cell toxicity. Methods : Rat insulinoma (RINm5F) cells were incubated with culture media (RPMI1640) in the presence of CsA and/or buthionine sulfoximine (BSO), which is an inhibitor of r-glutamyl cysteine synthetase, and reduced glutathione. The viable cells were examined using 3-〔4,5-dimethylthiazol-2-yl〕-2,5-diphenyl tetrazolium bromide (MTT) and was determined by a spectrophotometer at a wavelength of 570 nm. Results : Incubating the RINm5F cells with CsA resulted in a decrease in cell viability with increasing dose. This deceased cells viability, induced by CsA was potentiated by BSO treatment. The CsA and BSO induced cells toxicity was reduced significantly by the reduced glutathione. Conclusion : The results suggest that pancreatic β-cell may be injured by CsA and glutathione may have some role in cytotoxicity (J Kor Diabetes Asso 26:57~63, 2002).