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The Development Liquid Crystal Calibration Technique Using Neural Networks with Median Filtering
Lee, Dae-Hee,Boo, Kwang-Suk,Chung, Jae-Hun,Won, Se-Youl,Kim, Yun-Taek 인제대학교 1999 仁濟論叢 Vol.14 No.3
본 연구는 Neural networks와 median filtering을 사용함으로써 새로운 액정 교정기술을 개발하였고, 열전달 측정에 본 액정교정 기술을 응용하였다. 새로운 액정측정 기술의 확실성을 입증하기 위하여, 평판에 축대칭 충돌제트를 충돌시킬때 국소 Nusselt 수를 측정하였고, 같은 조건하에서 Hue 온도교정 기술에 의한 결과와 비교하였다. Neural networks는 온도와 R, G, B 값들 간에 비선형 관계를 예측할 수 있기 때문에 Neural network와 median filtering 교장기술은 실험에서 Hue 온도교정 기술보다 더 넓은 칼라구간을 사용할 수 있으며 실험시간면에서 현저한 감소의 결과를 가져올 수 있다. This study has developed a new liquid crystal calibration technique using Neural networks and median filtering and applied this technique to heat transfer measurements. To verify the validity of this new measurement technique, the local Nusselt numbers on a flat plate surface subjected to an axisymmetric impinging jet were measured and compared to the results by the conventional Hue-temperature calibration technique under the same conditions. Because Neural networks predict the non-linear relations between temperatures and corresponding R, G, B values, Neural networks-median filtering calibration technique can utilize a much wider color band in the experiment than the Hue-temperature calibration technique, resulting in the significant reduction in the experimental time.
Park, Chung Youl,Baek, Da Some,Oh, Jonghee,Choi, Jong-Yoon,Bae, Dae Hyeon,Kim, Jeong-Seon,Jang, Gil-Hun,Lee, Su-Heon The Korean Society of Plant Pathology 2016 식물병연구 Vol.22 No.2
Cymbidium mosaic virus (CymMV) is a major virus infecting orchid plants and causing economic loss. In this study, the incidence of viral infection in Calanthe spp. at the Korean Institute of Calanthe was investigated using reverse transcription polymerase chain reaction. The CymMV infection rate was 42%, and the two viruses Odontoglossum ringspot virus and Cucumber mosaic virus had frequencies of 8% and 2%, respectively. Additionally, we characterized an isolate of CymMV, CymMV-GW, using biological tests and examined the nucleotide sequence properties of its complete genome. CymMV-GW induced chlorotic ringspots and chlorotic spot symptoms in inoculated leaves of Chenopodium amaranticolor and Nicotiana benthamiana, respectively. In this study, we have for the first complete genome sequence of CymMV-GW in Korea. The CymMV-GW genome was 6,225 nucleotides in length, excluding the poly-(A) tail, and showed whole-genome nucleotide and amino acid sequence identities of 97.7% and 100%, respectively, with the NJ-1 isolate of CymMV. Here, we report the complete genome sequence of the CymMV-GW isolate and viral infection rates for Calanthe spp. in Korea.
팔라듐 표면처리를 통한 Massive Spalling 현상의 억제
이대현 ( Dae Hyun Lee ),정보묵 ( Bo Mook Chung ),허주열 ( Joo Youl Huh ) 대한금속재료학회 ( 구 대한금속학회 ) 2010 대한금속·재료학회지 Vol.48 No.11
The reactions between a Sn-3.0Ag-0.5Cu solder alloy and electroless Ni/electroless Pd/immersion Au (ENEPIG) surface finishes with various Pd layer thicknesses (0, 0.05, 0.1, 0.2, 0.4 μm) were examined for the effect of the Pd layer on the massive spalling of the (Cu,Ni)6Sn5 layer during reflow at 235℃. The thin layer deposition of an electroless Pd (EP) between the electroless Ni (7 μm) and immersion Au (0.06 μm) plating on the Cu substrate significantly retarded the massive spalling of the (Cu,Ni)6Sn5 layer during reflow. Its retarding effect increased with an increasing EP layer thickness. When the EP layer was thin (≤0.1 μm), the retardation of the massive spalling was attributed to a reduced growth rate of the (Cu,Ni)6Sn5 layer and thus to a lowered consumption rate of Cu in the bulk solder during reflow. However, when the EP layer was thick (≥0.2 μm), the initially dissolved Pd atoms in the molten solder resettled as (Pd,Ni)Sn4 precipitates near the solder/(Cu,Ni)6Sn5 interface with an increasing reflow time. Since the Pd resettlement requires a continuous Ni supply across the (Cu,Ni)6Sn5 layer from the Ni(P) substrate, it suppressed the formation of (Ni,Cu)3Sn4 at the (Cu,Ni)6Sn5/Ni(P) interface and retarded the massive spalling of the (Cu,Ni)6Sn5 layer.
A New Liquid Crystal Color Calibration Technique Using Neural Networks and Median Filtering
Lee, Dae-Hee,Chung, Jae-Hun,Won, Se-Youl,Kim, Yun-Taek,Boo, Kwang-Suk The Korean Society of Mechanical Engineers 2000 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.14 No.1
This study has developed a new liquid crystal calibration technique using Neural networks with median filtering and applied this technique to heat transfer measurements. To verify the validity of this new measurement technique, the local Nusselt numbers on a flat plate surface subjected to an axisymmetric impinging jet were measured and compared with the results by the conventional Hue-temperature calibration technique under the same conditions. Because the Neural networks predict the non-linear relations between temperatures and corresponding R, G, B values, Neural networks-median filtering calibration technique can utilize a much wider color band in the experiment than the Hue-temperature calibration technique, resulting in a significant reduction in the experimental time.
녹색 형광단백 유전자를 함유한 아데노바이러스 주입시 마우스 생체내 발현양상
진종률(Jong Youl Jin),송치원(Chi Won Song),김진아(Jea Na Kim),이희진(Hee Jin Lee),김태규(Tai Gyu Kim),한치화(Chi Wha Han),엄현석(Hyun Seok Eom),박수정(Soo Jeong Park),정대철(Dae Chul Jeong),정낙균(Nak Gyun Chung),김소연(Soh Yeon Kim) 대한내과학회 2001 대한내과학회지 Vol.61 No.5
N/A Background : The green fluorescent protein (GFP) from jelly fish, A equorea victoria, has become a versatile reporter for monitoring gene expression in a variety of cells and organisms . Using GFP as a marker protein we studied whether there are any differencies in the expression patterns among organs in mouse after intravenous injection of adenovirus vectors with GFP gene. Methods : Recombinant E1, E3- defective type 5 adenovirus vectors (2×10(8)/mouse) with CMV promoter and GFP gene were injected into mice via tail vein. On 3, 6, 9, 14, 21, 28 days after gene transfer, 5 mice per experiment s were sacrificed by cervical dislocation and obtained liver , lung, heart , kidney, spleen, small intestine and bone. Half of them were examined by optical microscope after H-E stain. Another half were examined by fluorescent microscope after frozen section. Western blotting were done for each samples with anti- GFP monoclonal antibody and obtained GFP bands were quantitatively compared using Gel-Doc (Bio- Rad, USA) image analyzer. Results : In all organs that we obtained, expression of GFPs are noticed 3 days after gene transfer and reached a maximum around 9th to 14th days, after then the intensities are slightly decreased but maintained until 28th days as determined by Western blotting. On fluorescent microscopic examination, GFPs are well and most frequently expressed on lung among all the examined organs. There are little expression of GFPs on liver parenchymal area around the sinusoids and central veins, although patchy expression of GFPs are observed along the liver capsules. GFPs are highly expressed around the splenic trabecula area but splenic pulp area, it is very spar sely expressed. GFPs are more frequently and highly expressed around the renal tubular area than gromerular area in kidneys. In small intestine, GFPs are expressed on mid portion of microvilli. GFPs are not expressed on myocardium except scanty expression on endocardium. Bone marrow showed GFPs but precise localization is difficult because bony spicules mashed bone marrow during the preparation of frozen section. No specific pathologic lesions possibly related with adenovirus administration are observed on microscopic examination of H-E stained specimens. Conclusions: GFPs can be detected in cells without the fixing and staining and a good marker to studying the kinetics and persistence of adenovirus mediated gene therapy. And there are different GFP expression patterns according to the organs after intravenous injection of adenovirus vectors with GFP gene in mouse.(Korean J Med 61:537- 545, 2001)